Neuroembryology and aging最新文献

Subject Index Vol. 5, 2008 主题索引，2008年第5卷

Neuroembryology and aging Pub Date : 2010-02-01 DOI: 10.1159/000284354

Jinping Liu, Xiulai Gao, Y. Wu, Song-fang Chen, Jingru Hu, Hong-yu Gu, Wen-long Ding, Shu-juan Yuan, Mei-fang Zhong, Yong Li, Lin Zhou, Li Liu, Juan Bao, Hong-bo Luo, W. Chan, C. Xia, D. Yew, Hui Zhao, S. Mok, D.T.S. Fang, Zhenze Liu, Zhuo Yang, Xiao-tong Wang, Kwok-hang Chung, E. Cho, Sheng-min Shao, C. Lai, K. Ng, K. Yung, D. Shum, Y. Chan, M. Wai, Lihong Zhang, D. Yew, Qi-dong Yang

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Author Index Vol. 5, 2008 作者索引2008年第5卷

Neuroembryology and aging Pub Date : 2010-02-01 DOI: 10.1159/000284353

引用次数: 0

Contents Vol. 5, 2008 目录2008年第5卷

Neuroembryology and aging Pub Date : 2010-02-01 DOI: 10.1159/000284370

Jinping Liu, Xiulai Gao, Y. Wu, Song-fang Chen, Jingru Hu, Hong-yu Gu, Wen-long Ding, Shu-juan Yuan, Mei-fang Zhong, Yong Li, Lin Zhou, Li Liu, Juan Bao, Hong-bo Luo, W. Chan, C. Xia, D. Yew, Hui Zhao, S. Mok, D.T.S. Fang, Zhenze Liu, Zhuo Yang, Xiao-tong Wang, Kwok-hang Chung, E. Cho, Sheng-min Shao, C. Lai, K. Ng, K. Yung, D. Shum, Y. Chan, M. Wai, Lihong Zhang, D. Yew, Qi-dong Yang

引用次数: 0

Study of Neuronal Apoptosis and Changes in Expression of the Proteins Related to the Mitochondrial Pathway after Aβ1–42 Injection in the Rat Hippocampus Aβ1-42注射后大鼠海马神经元凋亡及线粒体通路相关蛋白表达变化的研究

Neuroembryology and aging Pub Date : 2009-12-29 DOI: 10.1159/000270553

Juan Bao, Qi-dong Yang, Lin Zhou, Hong-bo Luo

{"title":"Study of Neuronal Apoptosis and Changes in Expression of the Proteins Related to the Mitochondrial Pathway after Aβ1–42 Injection in the Rat Hippocampus","authors":"Juan Bao, Qi-dong Yang, Lin Zhou, Hong-bo Luo","doi":"10.1159/000270553","DOIUrl":"https://doi.org/10.1159/000270553","url":null,"abstract":"The objective was to study neuronal apoptosis and changes in the proteins related to the mitochondrial pathway in the hippocampus of a rat model of Alzheimer’s disease (AD) and discuss its significance in AD. Thirty-six Sprague-Dawley rats were divided into 3 groups: a normal group, a control group and an AD group. AD was established by injecting β-amyloid1–42 (Aβ1–42) into the hippocampus. Learning and memory ability was estimated with a Y maze at different times. The apoptotic neurons were detected by the TdT-mediated X-dUTP nick end labeling (TUNEL) method. Cytochrome C and caspase-9 protein expression in the hippocampus was detected by Western blot. The results showed that there was obvious impairment of learning and memory in rats of the AD group after Aβ1–42 injection. The apoptotic neurons in the hippocampus of the rats in the AD group were significantly increased compared with the normal group and the control group (p < 0.01), and the expression of cytochrome C and caspase-9 in hippocampus in AD group was significantly increased too (p < 0.01). The results suggest that the mitochondrial pathway is one of the important neuronic apoptotic pathways induced by Aβ1–42 in the hippocampus, and may play an important role in the pathogenesis of AD.","PeriodicalId":89607,"journal":{"name":"Neuroembryology and aging","volume":"5 1","pages":"156 - 160"},"PeriodicalIF":0.0,"publicationDate":"2009-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000270553","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64963275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Chronic Hypoxic Hypercapnia on the Expression of Bcl-2 and Bax Genes and Apoptosis in Rat Hippocampal Neurons 慢性低氧高碳酸血症对大鼠海马神经元Bcl-2、Bax基因表达及凋亡的影响

Neuroembryology and aging Pub Date : 2009-12-29 DOI: 10.1159/000270902

Sheng-min Shao, Song-fang Chen, Yong Li, Xiao-tong Wang

{"title":"Effect of Chronic Hypoxic Hypercapnia on the Expression of Bcl-2 and Bax Genes and Apoptosis in Rat Hippocampal Neurons","authors":"Sheng-min Shao, Song-fang Chen, Yong Li, Xiao-tong Wang","doi":"10.1159/000270902","DOIUrl":"https://doi.org/10.1159/000270902","url":null,"abstract":"The objective of this paper is to explore the effect of chronic hypoxic hypercapnia on the apoptosis of rat neural cells in the hippocampus, as well as the relationship between apoptosis and Bcl-2 and Bax gene expression. In this study, 50 male Sprague-Dawley rats were randomly divided into 5 groups: a normal control group, and 4 hypoxic hypercapnia groups which received hypoxic hypercapnia treatment for 1, 2 , 3 and 4 weeks. The in situ apoptosis in the hippocampus was detected by TUNEL staining. The expression of bcl-2 and bax mRNA in the hippocampus was examined by RT-PCR. Results showed that the apoptosis index (AI) and expression of Bax mRNA increased with the duration of hypoxic hypercapnia, and that the expression of Bcl-2 mRNA increased at the beginning but then declined. There was a significant positive correlation between AI and the expression of Bax mRNA (r = 0.814, p < 0.01), and there was a significant negative correlation between AI and the ratio of Bcl-2 mRNA/Bax mRNA (r = –0.405, p < 0.01). The results suggest that apoptosis can be induced by chronic hypoxic hypercapnia in the rat hippocampus. The ratio of Bcl-2/Bax declined gradually, which accelerates the occurrence of apoptosis.","PeriodicalId":89607,"journal":{"name":"Neuroembryology and aging","volume":"5 1","pages":"161 - 165"},"PeriodicalIF":0.0,"publicationDate":"2009-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000270902","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64964308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Correlation between nNOS Expression and Erectile Dysfunction in Experimental Diabetes Mellitus Rats of Different Ages 不同年龄实验性糖尿病大鼠nNOS表达与勃起功能障碍的关系

Neuroembryology and aging Pub Date : 2009-04-29 DOI: 10.1159/000214864

Jin-jia Hu, Hong-yu Gu, Wen-long Ding, Shu-juan Yuan, Mei-fang Zhong, Li Liu

{"title":"Correlation between nNOS Expression and Erectile Dysfunction in Experimental Diabetes Mellitus Rats of Different Ages","authors":"Jin-jia Hu, Hong-yu Gu, Wen-long Ding, Shu-juan Yuan, Mei-fang Zhong, Li Liu","doi":"10.1159/000214864","DOIUrl":"https://doi.org/10.1159/000214864","url":null,"abstract":"Objective: To assess the correlation between neuronal nitric oxide synthase (nNOS) expression in the brain, lumbosacral spinal cord, sympathetic trunk, prostate and penis, and erectile dysfunction (ED) associated with diabetes mellitus (DM). Methods: DM was induced in adult male Sprague-Dawley rats by injecting streptozotocin. After 1, 2, 3 and 4 months, apomorphine was injected to test the penile reflex. The animals were then sacrificed, and the corpora cavernosa were isolated for a diastolic function test. In addition, the cortex of the brain, lumbosacral spinal cord, thoracic and lumbar sympathetic trunk, and root of the penis and prostate of each animal were subjected to a nNOS catalytic activity assay. Results: The times of penile reflex decreased gradually with the prolongation of DM. Acetylcholine-induced corpus cavernosum relaxation also markedly decreased in DM rats. On the other hand, in DM rats nNOS expression was elevated in the temporal cortex, hippocampus and hypothalamus, but was reduced in the spinal cord, sympathetic trunk and prostate. Statistics showed that nNOS expression in the brain correlated negatively with penis reflex and corpus cavernosum relaxation, while nNOS expression in the spinal cord, sympathetic trunk, penis and prostate had a positive correlation with penis reflex and corpus cavernosum relaxation. Conclusion: The expression of nNOS in the central and peripheral nervous systems correlated with diabetic ED and was a cause of diabetic ED.","PeriodicalId":89607,"journal":{"name":"Neuroembryology and aging","volume":"5 1","pages":"144 - 150"},"PeriodicalIF":0.0,"publicationDate":"2009-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000214864","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64338691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Coexpression of GABABR1 and GABABR2 Receptor Subunits in the Rat Vestibular Nucleus GABABR1和GABABR2受体亚基在大鼠前庭核中的共表达

Neuroembryology and aging Pub Date : 2009-01-20 DOI: 10.1159/000193850

C. Lai, K. Ng, Ken K.L. Yung, D. Shum, Ying-Shing Chan

{"title":"Coexpression of GABABR1 and GABABR2 Receptor Subunits in the Rat Vestibular Nucleus","authors":"C. Lai, K. Ng, Ken K.L. Yung, D. Shum, Ying-Shing Chan","doi":"10.1159/000193850","DOIUrl":"https://doi.org/10.1159/000193850","url":null,"abstract":"The expression patterns of the two subunits of the metabotropic GABAB receptor, GABABR1 and GABABR2, vary among various neural regions and their relative expression in the rat central vestibular system remains unknown. To identify the expression patterns of these two subunits of the metabotropic GABAB receptor in the vestibular nucleus of adult rats, double immunofluorescence experiments were performed. GABABR1 or GABABR2 immunoreactivity was observed in neurons and neuropilar elements of the vestibular nuclear complex and its subgroups x and y. These neurons were oval, multipolar, fusiform or triangular in shape. In contrast to other brain regions, individual neurons or neuropil within the various vestibular subnuclei and subgroups displayed comparable levels of GABABR1 and GABABR2 immunoreactivity. Double immunofluorescence experiments further demonstrated the coexpression of GABABR1 and GABABR2 receptor subunits in individual central vestibular neurons. The present findings provide histological evidence of the heterodimeric nature of GABAB receptors that mediate inhibitory neurotransmission in the rat vestibular nuclei.","PeriodicalId":89607,"journal":{"name":"Neuroembryology and aging","volume":"5 1","pages":"127 - 133"},"PeriodicalIF":0.0,"publicationDate":"2009-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000193850","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65297020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

N-Methyl-D-Aspartate Receptors Mediate Excitotoxicity in Amyloid Beta-Induced Synaptic Pathology of Alzheimer’s Disease n -甲基- d -天冬氨酸受体介导淀粉样蛋白β诱导的阿尔茨海默病突触病理的兴奋毒性

Neuroembryology and aging Pub Date : 2009-01-20 DOI: 10.1159/000193851

Jinping Liu, Xiulai Gao, Yan Wu

引用次数: 2

Ganglionic Eminence Revisited: A Review Embracing Observations from Our Laboratory 重新审视神经节隆起:回顾我们实验室的观察结果

Neuroembryology and aging Pub Date : 2009-01-20 DOI: 10.1159/000193226

M. Wai, Lihong Zhang, D. Yew

引用次数: 1

Effects of NMDA Receptors in Synapses and Extrasynapses of Rat Hippocampal Neurons on Amyloid-Beta-Induced Alterations in PSD-95 Expression 大鼠海马神经元突触和突触外NMDA受体对淀粉样蛋白诱导的PSD-95表达改变的影响

Neuroembryology and aging Pub Date : 2008-12-01 DOI: 10.1159/000183034

Jinping Liu, Yan Wu, Lirong Chang, Jing-ting Peng, Yizhi Song, Tao Lu, Xiulai Gao

{"title":"Effects of NMDA Receptors in Synapses and Extrasynapses of Rat Hippocampal Neurons on Amyloid-Beta-Induced Alterations in PSD-95 Expression","authors":"Jinping Liu, Yan Wu, Lirong Chang, Jing-ting Peng, Yizhi Song, Tao Lu, Xiulai Gao","doi":"10.1159/000183034","DOIUrl":"https://doi.org/10.1159/000183034","url":null,"abstract":"The objective of this study was to investigate the mechanism of the soluble Aβ oligomer-induced alteration in synaptic proteins. Therefore, an immunofluorescence technique was applied to investigate the changes in the expression of postsynaptic density-95 (PSD-95) protein in primary hippocampal neurons, which was exposed to Aβ25–35 after N-methyl-D-aspartate receptor (NMDAR) antagonist or agonist treatment. Results showed that Aβ25–35 downregulated PSD-95 protein in a dose- and time-dependent manner. Treatment of cells with MK-801 (a general NMDA receptor antagonist) prevented Aβ-induced PSD-95 degradation. Moreover, when extrasynaptic NMDA receptors were blocked by ifenprodil (a specific antagonist of the NR2B subunit), the Aβ-induced downregulation of PSD-95 was significantly attenuated. However, when synaptic NMDA receptors were blocked by bicuculline (a GABA receptor antagonist) in combination with MK-801, the PSD-95 degradation did not change significantly. The results suggest that Aβ-induced downregulation of PSD-95 depends on NMDAR activity, and extrasynaptic NMDA receptors may be involved in Aβ-induced synaptic protein degradation.","PeriodicalId":89607,"journal":{"name":"Neuroembryology and aging","volume":"5 1","pages":"116 - 120"},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000183034","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65143292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2