Bioscience Journal最新文献_第5页

Potential of rust-resistant arabica coffee cultivars for specialty coffee production 抗锈蚀阿拉比卡咖啡品种在特种咖啡生产中的潜力

IF 0.5 4区农林科学

Bioscience Journal Pub Date : 2023-03-31 DOI: 10.14393/bj-v39n0a2023-66103

Tharyn Reichel, M. L. V. Resende, D. H. S. Nadaleti, Fábio de Oliveira Santos, C. E. Botelho

{"title":"Potential of rust-resistant arabica coffee cultivars for specialty coffee production","authors":"Tharyn Reichel, M. L. V. Resende, D. H. S. Nadaleti, Fábio de Oliveira Santos, C. E. Botelho","doi":"10.14393/bj-v39n0a2023-66103","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-66103","url":null,"abstract":"Rust is the main disease affecting Coffea arabica, the most economically important coffee species. The objective of this study was to analyze C. arabica cultivars with different levels of rust resistance, including bean size, raw bean appearance, final sensory scores (FSS), and aroma and taste nuances of the coffee cup. The experiment was designed in randomized blocks (RBD) with three replications and 20 treatments (cultivars), totaling 60 experimental plots. The rust-susceptible cultivars IPR 100, Rubi MG 1192, and Topázio MG 1190 were compared with 17 rust-resistant cultivars. Cultivars IPR 103, MGS Aranãs, and Saíra II presented the highest percentages of high sieves, highest scores of raw bean appearance, and low percentages of mocha-type beans. All cultivars had FSS above 82 and were classified as specialty coffees. The cultivars with the highest FSS (Arara and Catiguá MG2) showed a greater diversity of coffee cup aroma and flavor nuances. Rust-resistant Arabica coffee cultivars are promising for the physical quality of beans and have potential for the specialty coffee market.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43369068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Productivity and mortality, of Fusarium oxysporum f. sp. passiflorae in passionfruit vines brs rubi do cerrado, grafted onto different rootstocks 将尖孢镰刀菌（Fusarium oxysporum f.sp.passiflorae）嫁接到不同砧木上的百香果藤（brs rubi do cerrado）的生产力和死亡率

IF 0.5 4区农林科学

Bioscience Journal Pub Date : 2023-03-31 DOI: 10.14393/bj-v39n0a2023-61288

Luciano Gomes Ferreira, F. C. Juliatti, M. F. Moraes, L. Kobayasti, G. Roncatto, B. Juliatti, Risely Ferraz de Almeida, Carlos Antônio Távora Araújo

{"title":"Productivity and mortality, of Fusarium oxysporum f. sp. passiflorae in passionfruit vines brs rubi do cerrado, grafted onto different rootstocks","authors":"Luciano Gomes Ferreira, F. C. Juliatti, M. F. Moraes, L. Kobayasti, G. Roncatto, B. Juliatti, Risely Ferraz de Almeida, Carlos Antônio Távora Araújo","doi":"10.14393/bj-v39n0a2023-61288","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-61288","url":null,"abstract":"The use of scions grafted onto passionfruit vines has become an alternative to their cultivation in areas with a history of fusariosis. However, the combinations between the graft and rootstock can influence on the productivity and longevity of the passionfruit farm. The objective was to evaluate the productivity and mortality of the passionfruit cv BRS Rubi do Cerrado grafted onto three species of rootstock in Terra Nova do Norte-MT. The design was a randomized block with 4 treatments BRS Rubi do Cerrado (BRS-RC) seedling plant along with those grafted onto three species of rootstock: Passiflora gibertii, Passiflora alata; Passiflora nitida. The scions were grafted through a cleft graft and the planting performed after 70 days. The evaluation was made of the total number and weight of the fruits, along with productivity and mortality of plants. Results showed that the BRS-RC, grafted onto P. gibertii and P. nitida, presented the best performance for weight of fruits, number of fruits and productivity. The cultivar that was grafted onto different rootstocks presented higher productivity in regards to the seedling plant. The BRS-RC, grafted onto P. nitida, presented a zero-mortality rate over the 16.5 months of cultivation. The rootstocks P. nitida and P. gibertii were superior to those of P. alata in reducing mortality in the passionfruit vine by Fusarium oxysporum f. sp. passiflorae. The confirmation was made of the pathogen Fusarium oxysporum f. passiflorae isolated in the experimental area in scions of the BRS-RC inoculated at 70 days of age.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46139113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Does the mycotoxin ingestion by beef heifers on feedlot change the productive parameters? 肉牛在饲养场摄入霉菌毒素是否会改变生产参数?

IF 0.5 4区农林科学

Bioscience Journal Pub Date : 2023-03-31 DOI: 10.14393/bj-v39n0a2023-64329

Danielle de Oliveira Pião, M. Mello, M. M. D. Barbero, A. L. Silva, A. M. Moura, R. P. Barbero

{"title":"Does the mycotoxin ingestion by beef heifers on feedlot change the productive parameters?","authors":"Danielle de Oliveira Pião, M. Mello, M. M. D. Barbero, A. L. Silva, A. M. Moura, R. P. Barbero","doi":"10.14393/bj-v39n0a2023-64329","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-64329","url":null,"abstract":"In intensive beef cattle production systems, silage, corn, soy bean, and their coproducts are commonly used as feed. However, these ingredients are highly susceptible to contamination by fungi and mycotoxins, which may lead to immunological challenges and reduce animal production. The aim of the present study was to evaluate the effects of mycotoxin contamination of diet on intake, digestibility, and performance of heifers. Twenty non-pregnant (Nellore) heifers (age, >18 months; initial body weight, 348±30 kg) were used and randomly distributed in two treatments: (1) control (non-contaminated diet) and (2) zearalenone-contaminated diet (300 ppb). The diet comprised 70% corn silage and 30% concentrate. Individual dry matter intake and digestibility were estimated using external and internal markers. Heifer body weight was evaluated every week without fasting to calculate performance. The experimental design was completely randomized. Each animal was considered one experimental unit. Assumptions were tested for variance analyses (error normality, independence of errors, and homogeneity of variances) (p<0.05). There were no differences in dry matter intake (p=0.96) and digestibility (p=0.62). Performance (kg/day) did not vary as a function of zearalenone ingestion (p=0.68). Therefore, contamination of diet with 300 ppb zearalenone did not affect the intake, digestibility, and performance of feedlot-finished heifers.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49047543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial activity of Mangifera indica seed extracts combined with common antibiotics against multidrug-resistant Pseudomonas aeruginosa and Acinetobacter baumannii isolates 芒果籽提取物联合常用抗生素对多重耐药铜绿假单胞菌和鲍曼不动杆菌的抑菌活性

IF 0.5 4区农林科学

Bioscience Journal Pub Date : 2023-03-31 DOI: 10.14393/bj-v39n0a2023-66760

Shadi Zeraatkar, Maedeh Tahan, Omefarveh Rostami, Alireza Neshani, Hadi Farsiani, Arezou Shahsavari, H. Safdari, Mahdi Hosseini Bafghi

{"title":"Antibacterial activity of Mangifera indica seed extracts combined with common antibiotics against multidrug-resistant Pseudomonas aeruginosa and Acinetobacter baumannii isolates","authors":"Shadi Zeraatkar, Maedeh Tahan, Omefarveh Rostami, Alireza Neshani, Hadi Farsiani, Arezou Shahsavari, H. Safdari, Mahdi Hosseini Bafghi","doi":"10.14393/bj-v39n0a2023-66760","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-66760","url":null,"abstract":"In this project, we employed ethanolic (EMI) and aqueous (AMI) extracts of mango (Mangifera indica L., Anacardiaceae) fruit seeds as a modulator of antibiotic resistance against multidrug-resistant (MDR) Pseudomonas aeruginosa and Acinetobacter baumannii to evaluate natural compounds isolated from by-products or waste of edible plants. We also investigated the effect of these extracts alone and in combination with standard classes of antibiotics in the desired strains. M. indica seeds were processed and exploited using ethanol and water. The minimum inhibitory concentrations (MICs) of clinical isolates were examined against EMI and AMI extracts, followed by seven antibiotics of ceftazidime, ciprofloxacin, penicillin, amikacin, meropenem, ampicillin, and colistin. The checkerboard method evaluated the synergistic action between mango kernel extract (EMI) and seven antibiotics. EMI extract significantly revealed antimicrobial properties against MDR A. baumannii and P. aeruginosa with synergistic effects with the applied antibiotics. The considerable antibacterial efficacy of ethanolic extract of M. indica seeds can have great curative value as antibacterial drugs against infections caused by MDR P.aeruginosa and A. baumannii.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41952313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quality of raw bulk-tank milk produced in northeast Brazil 巴西东北部生产的原料散装罐牛奶的质量

IF 0.5 4区农林科学

Bioscience Journal Pub Date : 2023-03-31 DOI: 10.14393/bj-v39n0a2023-59825

V. M. D. Araújo, S. Barbosa, A. H. Rangel, Luís H F Borba, Juliana Oliveira, Â. Batista

{"title":"Quality of raw bulk-tank milk produced in northeast Brazil","authors":"V. M. D. Araújo, S. Barbosa, A. H. Rangel, Luís H F Borba, Juliana Oliveira, Â. Batista","doi":"10.14393/bj-v39n0a2023-59825","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-59825","url":null,"abstract":"The objective of this study was to evaluate the seasonal influence on the chemical composition, somatic cell count (SCC), and total bacterial count (TBC) of raw bulk-tank milk in northeastern Brazilian states. Data were obtained from milk samples from tanks collected monthly by industries registered with the Federal Inspection Service. According to normative instruction #62 (IN-62), two validity periods were considered. The highest recorded averages for chemical composition were between May and July. The mean fat content varied from 3.51% to 3.69%, and the protein content ranged from 3.07% to 3.17%. The averages of SCC ranged from 4.66 to 4.90 × 1,000 cells/ml, with the highest being recorded in July. At the same time, the TBC ranged from 2.34 to 2.53 cfu/ml. The highest TBC was recorded in March. The mean values of fat, protein, defatted dry extract, SCCs, and TBC were influenced by the months of the year. The means for these variables decreased in periods when Brazilian legislation was more severe. However, the SCC and TBC averages found in this study were still high, considering the quality of raw milk production. SCC and TBC presence still did not comply with the limits established by the legislation.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42226920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tifton 85 grass performance following chemical and organic fertilizers 施用化学和有机肥料后的蒂夫顿85草的生长性能

IF 0.5 4区农林科学

Bioscience Journal Pub Date : 2023-03-31 DOI: 10.14393/bj-v39n0a2023-63730

Naiara Taís Alves da Silva, A. Teixeira, L. Gonçalves, D. Ottoni, L. Cavalcanti, Thaís Figueiredo Pereira, Flávia Cristina de Oliveira e Silva, D. Jayme

{"title":"Tifton 85 grass performance following chemical and organic fertilizers","authors":"Naiara Taís Alves da Silva, A. Teixeira, L. Gonçalves, D. Ottoni, L. Cavalcanti, Thaís Figueiredo Pereira, Flávia Cristina de Oliveira e Silva, D. Jayme","doi":"10.14393/bj-v39n0a2023-63730","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-63730","url":null,"abstract":"The Tifton 85 grass offers high yield and nutritional value. It is demanding in soil fertility and highly responsive to fertilization. However, the response to fertilization can be affected by the source, dose, and installment of fertilizers, as well as environmental conditions. Evaluations of the joint application of chemical and organic fertilizers are scarce. Therefore, this study aimed to evaluate the effect of chemical and organic fertilization on agronomic characteristics, mineral use efficiencies, and bromatological composition of Tifton 85 grass, and determine fertilization strategies to optimize the yield and nutritional value of Tifton 85 grass. The findings were influenced by grass cuttings. The highest values for height and accumulated dry matter productivity were obtained with the highest fertilizer doses. The leaf/stem ratio was not affected by fertilization. The highest mineral use efficiencies were obtained with 200 and 400 kg of N/ha/year of NPK 20-00-20 formulation and up to 10 t/ha/year of poultry litter (PL), and these values decreased when applying high PL doses. The crude protein content increased, while the contents of neutral detergent fiber and acid detergent fiber decreased. The protein fractions and in vitro dry matter digestibility were not affected by fertilization. The potassium content increased with high fertilizer doses. Applying 350 to 400 kg of N/ha/year of NPK 20-00-20 formulation and 25 to 30 t/ha/year of PL promoted the highest accumulated dry matter productivity. Applying 200 to 400 kg of N/ha/year of NPK 20-00-20 formulation and 10/ha/year of PL is recommended for optimizing mineral use efficiencies. As for bromatological composition, increasing the doses of both fertilizers was beneficial in reducing fiber contents, but only organic fertilization increased crude protein content.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42270755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation and characterization of steam and CO2 activated carbon from Brazil nut shell 巴西坚果壳蒸汽活性炭和CO2活性炭的制备及表征

IF 0.5 4区农林科学

Bioscience Journal Pub Date : 2023-03-31 DOI: 10.14393/bj-v39n0a2023-64438

Pryscila Machado de Castro, Stela Regina Ferrarini, Michelle Ferreira da Silva Rimoli, A. A. Merlo, R. M. Nogueira, E. M. Pires

引用次数: 0

Proposal of a non-linear model to adjust in vitro gas production at different incubation times 提出一种非线性模型来调整不同孵育时间的体外产气量

IF 0.5 4区农林科学

Bioscience Journal Pub Date : 2023-03-31 DOI: 10.14393/bj-v39n0a2023-63017

A. L. B. D. Santos, T. A. Ferreira, C. Brito, G. R. Moreira, Frank Gomes-Silva, J. Jale, R. B. Reis, Leonardo Andrade Leite, P. G. Pimentel

{"title":"Proposal of a non-linear model to adjust in vitro gas production at different incubation times","authors":"A. L. B. D. Santos, T. A. Ferreira, C. Brito, G. R. Moreira, Frank Gomes-Silva, J. Jale, R. B. Reis, Leonardo Andrade Leite, P. G. Pimentel","doi":"10.14393/bj-v39n0a2023-63017","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-63017","url":null,"abstract":"This work aims to propose a new model named Gompertz-Von Bertalanffy bicompartmental (GVB), a combination of the models Gompertz and Von Bertalanffy. The GVB models is applied to fit the kinetic curve of cumulative gas production (CGP) of four foods (SS – sunflower silage; CS – corn silage; and the mixtures 340SS – 660 gkg-1 of corn silage and 340 gkg-1 of sunflower silage; and 660SS – 340 gkg-1 of corn silage and 660 gkg-1 of sunflower silage). The GVB fit is compared to models Logistic-Von Bertalanffy bicompartmental (LVB) and bicompartmental logistic (BL). All the process studied employed the semi-automatic “in vitro” technique of producing gases used in ruminant nutrition. The gas production readout was performed at times 2, 4, 6, 8, 10, 12, 15, 19, 24, 30, 48, 72, and 96 h. The data generated were used to estimate the models’ parameters by the least squared method with the iterative Gauss-Newton process. The data fit quality of the models was verified using the adjusted coefficient of determination criterion (), mean residual square (MRS), Akaike information criterion (AIC), and mean absolute deviation (MAD). Among the analyzed models, the LVB model presented the best quality of fit evaluators for CS. In contrast, the GVB model showed better quality of fit to describe CGP over time for 340SS, 660SS, and SS, presenting the highest values of () and the lowest values of MSR, AIC, and MAD.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44664733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anatomical, morphogenic and structural characteristics of Xaraés palisade grass under grazing 放牧条件下沙拉萨草的解剖、形态及结构特征

IF 0.5 4区农林科学

Bioscience Journal Pub Date : 2023-03-31 DOI: 10.14393/bj-v39n0a2023-60937

Kelen Cristina Basso, L. Galzerano, W. L. Silva, A. Ruggieri, R. Reis

{"title":"Anatomical, morphogenic and structural characteristics of Xaraés palisade grass under grazing","authors":"Kelen Cristina Basso, L. Galzerano, W. L. Silva, A. Ruggieri, R. Reis","doi":"10.14393/bj-v39n0a2023-60937","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-60937","url":null,"abstract":"This study evaluated under grazing intensities and periods of the year: leaf anatomy of Urochloa brizantha cv. Xaraés and its correlation with morphogenetic and structural characteristics, and leaves degradation after in situ incubation. Treatments were four grazing intensities (GI) defined by the pasture residuals leaf area index (rLAI 0.8, 1.3, 1.8, and 2.3) with three replications in a completely randomized design. Cows grazed in a rotational stocking with pastures regrowth period determined by 95% light interception. Leaves showed a higher proportion of sclerenchyma (2.64%) in pastures under lower GI and in the dry season (2.42%). Pastures managed under higher GI showed lower number of expanded leaves (2.58), lower number of lives leaves (3.45), and lower leaf senescence rate (0.05 cm tiller−1 d−1). Positive correlation was observed between leaf elongation rate and adaxial epidermis and vascular tissues. rLAI 1.8 and 2.3 provided greater residues after in situ leaf incubation at times 12, 48, 72, and 96 h compared to rLAI 0.8 and 1.3. rLAI and period of the year had little influence on leaf anatomy of the Xaraés managed under 95% LI, and leaf anatomy is correlated with the morphogenetic and structural pasture characteristics. Pastures managed under lower GI show more residues after leaves incubation in rumen.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41357424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibitory effects of diarsenic trioxide (As2O3) on hepatocellular carcinoma cells exerted by regulation of promyelocytic leukemia protein levels 三氧化二砷通过调节早幼粒细胞白血病蛋白水平对肝癌细胞的抑制作用

IF 0.5 4区农林科学

Bioscience Journal Pub Date : 2023-03-31 DOI: 10.14393/bj-v39n0a2023-63086

Guowu Zhang, Wen Wang, Yukai Jin, Shi Jin, Lei Mi, Xiaowen Song, He Li, J. Liao

{"title":"Inhibitory effects of diarsenic trioxide (As2O3) on hepatocellular carcinoma cells exerted by regulation of promyelocytic leukemia protein levels","authors":"Guowu Zhang, Wen Wang, Yukai Jin, Shi Jin, Lei Mi, Xiaowen Song, He Li, J. Liao","doi":"10.14393/bj-v39n0a2023-63086","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-63086","url":null,"abstract":"Previous Chinese research revealed that diarsenic trioxide (As2O3) inhibits acute promyelocytic leukemia (PML) cell proliferation and initiates apoptosis through degradation of the PML-retinoic acid receptor protein. This study was to analyse whether As2O3 also had an effect on hepatocellular carcinoma (HCC) cells. As2O3 effects on various HCC cell lines and primary HCC cells were investigated in time and dose series, including measurements of cell growth, PML mRNA and protein expression, xenografted tumor formation, and the self-renewal Oct4 and hepatocyte marker expressions in mouse model xenografts or cells treated with PML siRNA. The results were analyzed by immunocytochemistry, quantitative reverse transcription PCR and western blotting as well as indocyanine green and Periodic Acid Schiff staining. As2O3 inhibited HCC cell and HCC cell-derived xenograft tumor formation in a time-dependent manner and reduced PML protein expression in HCC cells, but had limited effects on PML mRNA levels in cell nuclei. The HCC cell line HuH7 treated with As2O3 showed a decreased expression of alpha-fetoprotein and increased expression and transcription of mature hepatocyte markers, indicating differentiation of HCC cells into hepatocytes. Cytokeratin 18 protein and mRNA levels as well as tyrosine aminotransferase and apolipoprotein B mRNA transcriptions were enhanced by As2O3 as were the numbers of indocyanine green and Periodic Acid Schiff stained cells. In addition, As2O3 downregulated the expression of Oct4. In conclusion, since As2O3 inhibited HCC cell proliferation and HCC cell-derived xenograft tumor formation it is suggested that an appropriate concentration of As2O3 might be a promising therapy to treat HCC.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46549975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0