Bioscience Journal最新文献

{"title":"Molecular analysis of the prevalence of Acinetobacter baumannii in hospitals and the surrounding environments: a cross-sectional study","authors":"Kerolly T. P. B. Silva, C. B. Miguel, Rony Rocha de Oliveira Júnior, M. C. Araújo, F. Agostinho, C. Ueira-Vieira, J. E. Lazo-Chica, W. Rodrigues","doi":"10.14393/bj-v39n0a2023-63071","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-63071","url":null,"abstract":"Acinetobacter baumannii is widely recognized in clinical environments due to its infectious capacity, antimicrobial adaptability, and lethality. Analyzing the prevalence of this agent in intra- and extra-hospital environments may reveal target indicators for appropriate management interventions. In this observational cross-sectional study, we evaluated the prevalence of A. baumannii within hospitals with intensive care units and in their external surroundings in a macro-health region of Brazil. Samples of Columba livia (pigeon) droppings from the external environment of four hospitals (n = 40), from floor surfaces (n = 20), and door handles (n = 20) of different hospital wards were collected based on random sampling, all of which were evaluated for the presence of A. baumannii using polymerase chain reactions (PCR). The sensitivity and specificity of the technique was verified after the collected samples were contaminated with clinical samples positive for A. baumannii. We detected a significantly higher A. baumannii prevalence (87.50%, CI = 71.29–100.00) in samples collected within the hospital environment compared with those obtained from the external environment (12.50%, CI = 0.00–28. 71) (p = 0.003). In addition, samples collected from floor surfaces contained bacterial densities (181.3 ± 11.58) that exceeded those in environmental (93.32 ± 1.56) and door handle (142.70 ± 17.14) samples by 94% and 78.71%, respectively. The findings of this study will enhance our understanding of the spatial distribution of A. baumannii and additionally, validate the efficiency of PCR for diagnosis of this infectious agent.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47052555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of lysine production by Escherichia coli and Klebsiella isolates at different carbon sources","authors":"N. Gilani, Tahira Maqbool, Muhammad Mohibullah, Ume Habiba, T. Anwar, Huma Qureshi","doi":"10.14393/bj-v39n0a2023-60568","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-60568","url":null,"abstract":"Lysine is an essential amino acid that is not biologically manufactured in the body. Different chemical methods for lysine production are expensive and give low yields. The present study was conducted with the purpose to evaluate the biochemical production of lysine by different carbon sources using bacterial isolates. Three carbon sources namely glucose, sucrose, and fructose were used to evaluate the biochemical production of lysine by Escherichia coli and Klebsiella spp. isolates. Optimum incubation periods were between 48-96 hours. An extensive amount of lysine was produced by all of these isolates in L6 fermentation medium. Maximum lysine was produced by Klebsiella isolate K1 6.48 g/L after 96 hours of incubation by using glucose as carbon source followed by 6.0 g/L by Klebsiella isolates K3 after 72 hours of incubation when sucrose was used as a carbon source at 37 °C. Highest amount of lysine was produced at 96 hours by Klebsiella isolates in addition to E. coli. From all three carbon sources using Klebsiella isolates and E. coli, glucose showed better lysine production.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48855409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Considering the role of the assassin snail Anentome helena as a biological control of Bithynia siamensis goniomphalos, the first intermediate host of Opisthorchis viverrini","authors":"Surat Haruay, S. Piratae","doi":"10.14393/bj-v39n0a2023-62710","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-62710","url":null,"abstract":"This study aims to investigate whether the assassin snail Anentome helena may serve as a biological control agent of Bithynia siamensis goniomphalos, the 1st intermediate host of Opisthorchis viverrini. Experiments were carried out in the laboratory, and the results found that A. helena showed the variation and selection of mollusc prey. A. helena can consume B. siamensis goniomphalos, which is remarkable because this snail can compete with other snails and could be used as a biological control. The consumption rate of the predator was compared, and it was found that A. helena prefers to consume Indoplanorbis exustus, followed by Pomacea canaliculata, Melanoides tuberculata, Filopaludina sumatrensis speciosa, Lymnaea sp., and B. siamensis goniomphalos. This is the first report of an experimental study controlling B. siamensis goniomphalos using A. helena. Our data imply that A. helena can control the B. siamensis goniomphalos population with good results, especially in the absence of other snail species.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41793954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effect of sorafenib with Platycladus orientalis (L) leaf extract on cervical cancer","authors":"Elvis Samuel, D. Wilson, Catherene Tomy, D. J. I. Nallakannu, Siddikuzaman, Aavany Balasubramanian, V. M. Berlin Grace","doi":"10.14393/bj-v39n0a2023-62558","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-62558","url":null,"abstract":"The extracts of Platycladus orientalis (L.) Franco leaves have shown promising anti-cancer, anti-oxidant and anti-inflammatory potency with the traditional knowledge of healing HPV associated warts. The purpose of this research is to assess the synergistic activity of sorafenib and Platycladus orientalis (L) leaf extraction on cervical cancer cells. The cytotoxicity efficiency of different concentrations of Sorafenib and ethanol extract of Platycladus orientalis (L.) leaves were tested on HeLa cells by MTT and Trypan blue assays. The synergistic effect of the IC50 concentrations of Sorafenib and Platycladus orientalis (L.) on HeLa cell by MTT assay, and mRNA expression levels of tumor suppressor tazarotene-induced gene 3 (TIG3), proliferating cell nuclear antigen (PCNA) gene and apoptosis modulator (Bcl-2) gene by RT-PCR were evaluated with individual treatments. Combination treatment showed a relatively more expression of TIG3 and less expression of Bcl-2 and PCNA was observed. Growth factor-induced MAPKP activation was arrested by compound combination treatment, which and suppression of proliferation-induced apoptosis of cervical cancer cells. Based on the our results, the combination of sorafenib and crude leaf extract from Platycladus orientalis (L.) can effectively suppress cervical cancer cell growth, thereby providing an interesting rationale for further clinical trials and in-vivo studies.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44750538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chronic kidney disease intervention in primary health care: integrative review","authors":"Andyara Thalissa Forin Paes, A. Barbieri, Andréia Insabralde de Queiroz Cardoso, M. A. Ferreira Júnior","doi":"10.14393/bj-v39n0a2023-62191","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-62191","url":null,"abstract":"To analyze the knowledge and safety of primary health care professionals in the management of chronic kidney disease in its early stages. Integrative literature review carried out in four steps. The search took place in the following databases: MEDLINE via PubMed, Web of Science, Scopus and BVS. The descriptors used were “Primary Health Care” AND “Kidney Diseases” AND “Health Knowledge, Attitudes, Practice” and the quality of the analysis was checked by means of the Hierarchy of Evidence for Intervention Studies”. Fifteen articles were found, with a predominance of qualitative and descriptive method; the results showed insufficient knowledge of health professionals and interest in the best approach in the initial stage of the disease. Thus, it is inferred that the knowledge and security of primary health care professionals depend on individual factors and institutional initiative for the adoption of clinical guidelines and training. Therefore, prepared professionals and organized care, using protocols, would bring benefits both to patients as to the evolution and outcome of the disease and  savings to health services.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42590929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of oral injuries and salivary changes in patients with chronic renal failure on hemodialysis: systematic review and meta-analysis","authors":"Vanessa Cardoso Dos Santos, D. J. Gambin, Keli Adriana Silvestre Casanova, F. C. Vitali, Letícia Copatti Dogenski, T. M. Duque, M. Trentin, João Paulo De Carli","doi":"10.14393/bj-v39n0a2023-63061","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-63061","url":null,"abstract":"The objective of this study was to evaluate whether individuals with chronic renal failure (CRF) undergoing hemodialysis treatment have more salivary and oral mucosa alterations when compared to healthy individuals, through a systematic review followed by meta-analysis. A systematic literature review was performed, evaluating randomized clinical trials found in the Proquest, Embase, Scopus, Cochrane Library, Web of Science, Lilacs and Pubmed databases, using MeSH terms and other keywords. Initially, 40 articles were included in the study and, after reading the complete articles, only 15 clinical trials that analyzed oral lesions and salivary changes in patients with CRF undergoing hemodialysis treatment were eligible. Most of the clinical studies included were cross-sectional and composed of a study group and a control group. The mean age of participants in the study group was 50.19 years and in the control group, 48.95 years. The most common oral alterations found in the CRF group in relation to the control group were xerostomia, uremic breath, dysgeusia, coated tongue, gingival bleeding and pale mucosa. The salivary flow of patients with CRF was 46.6% lower than the control group. The salivary pH in the study group was also more alkaline when compared to the control group. Greater amounts of urea, phosphate, C-reactive protein and total proteins were found in the saliva of individuals with CRF. Individuals with CRF undergoing hemodialysis are more prone to changes in both the quantity and quality of saliva, as well as having a greater amount of oral changes.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41904428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of low temperature and room temperature on the depth of cure of bulk-fill composite resins","authors":"Anna Flávia Esteves Ferreira, Ingrid Melo Silva, L. Dietrich, Marcelo Dias Moreira de Assis Costa, Cláudia Maria de Oliveira Andrade, Victor da Mota Martins","doi":"10.14393/bj-v39n0a2023-63528","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-63528","url":null,"abstract":"The study analyzed the influence of temperature on the depth of cure of bulk-fill composite resins. Three discs (ISO 4049/2000) from each group were made, and four restorative materials were investigated: Opus Bulk-Fill Flow, Opus Bulk-Fill APS, Filtek™ One Bulk-Fill, and Filtek™ Bulk-Fill Flow. They were light-cured (Gnatus ± 1,200 mW/cm2) for 20 seconds, varying the temperature (23°C and 5°C) and simulating the use of the product both at room temperature and under refrigeration. The materials were inserted in aluminum matrices with 10 mm in depth and 4 mm in diameter. A clear film strip and a glass microscope slide were positioned at the top and bottom surfaces. The material was condensed and light-cured with the tip of the light source in close contact, for 20 seconds on the top surface. Immediately after irradiation, the samples were removed from the mold, and the uncured part was removed with a plastic spatula. The measurement was performed with a micrometer ± 0.1 mm, and the value was divided by 2. The data were analyzed with two-way ANOVA at a significance of 0.05. There was a statistical difference in the temperature between the results obtained in Opus Bulk-Fill APS (p <0.001) and Filtek™ Bulk-Fill Flow (p = 0.018) resins. For the temperature of 5°C, Filtek™ Bulk-Fill Flow showed a statistical difference compared to the other resins, while Opus Bulk-Fill Flow and Opus Bulk-Fill APS did not. For the temperature of 23°C, Filtek™ Bulk-Fill Flow maintained the statistical difference from the others, but Filtek™ One Bulk-Fill and Opus Bulk-Fill APS did not present statistical differences. Storing the Filtek™ Bulk-Fill Flow composite resin in the refrigerator caused a greater depth of cure than the other resins, and the depth of cure decreased at room temperature. As for Opus Bulk-Fill APS, the depth of cure decreased proportionally to the temperature decrease. The temperature may affect the depth of cure of some composite resins.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41353072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Subjective oral health measures in caregivers of patients with autism spectrum disorder and down syndrome: a preliminary study","authors":"Juliana Vianna Pereira, Tamiris Christensen Bueno, F. Herkrath, Mirlena Mansur Dionizio da Silva, Milena Cardia Leme, A. Santos-Silva, M. Lopes","doi":"10.14393/bj-v39n0a2023-62137","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-62137","url":null,"abstract":"This study aimed to address the subjective oral health measures of caregivers of individuals with autism spectrum disorder (ASD) and Down syndrome. This cross-sectional study included 15 caregivers of individuals with ASD (n = 7) and Down syndrome (n = 8). Sociodemographic data, sense of coherence (SOC) (SOC-13 scale), social support (Social Support Scale [MOS-SSS]), oral health-related quality of life (OHRQoL) (OHIP-14), and self-rated oral health assessed on a 5-point Likert scale were collected. A descriptive analysis was performed in addition to correlation analyses (Spearman correlation coefficient). Most primary caregivers were parents of the individual (86.6%). The age of the caregivers ranged between 40 and 59 years (60%). Most caregivers have had 9 to 11 years of education (53.3%). Monthly family income was less than USD 186,28 for ASD caregivers (57.2%) and between USD 327,56 and USD 931,40 for Down syndrome caregivers (50%). Of the caregivers, 33.3% reported good oral health and 33.3% reported neither good nor bad. The average SOC score, social support and OHRQoL was 48.9, 69.3 and 10.9, respectively. The higher the family income, the better the OHRQoL (rs = -0.62, p = 0.014). SOC was correlated with the score of the emotional support domain (rs = 0.54, p = 0.039). It was concluded that caregivers had a strong SOC and high perceived support. Moreover, caregivers did not report a high impact on OHRQoL A better understanding of the caregivers’ protective and coping factors in caring for individuals with disabilities may better promote their quality of life.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49235716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiline aiming at phenotypic stability and rice blast resistance","authors":"D. G. Castro, A. M. Moura, N. B. Alves, L. M. Tomé, F. B. S. Botelho, A. R. Neto, Douglas Correa de Souza","doi":"10.14393/bj-v38n0a2022-59610","DOIUrl":"https://doi.org/10.14393/bj-v38n0a2022-59610","url":null,"abstract":"This study aimed to verify the efficiency of multilines in reducing blast progress and their potential benefits to phenotypic stability in rice. The experiments were conducted in the 2016/17 and 2017/18 agricultural years. A randomized block design was performed with three replications, evaluating 12 lines and a multiline, which consisted of five lines from the Cultivation and Use Value (CUV) test. The multiline presented an estimated grain yield above the average of experiments of around seven bags ha-1 and superior performance in early flowering, justifying the high phenotypic stability for these characters. In this case, the line selection for composing the multiline was favorable and efficient, highlighted by a higher agronomic performance than most lines of the CUV test. The multiline is an adequate strategy to provide higher phenotypic stability and reduce blast progress in the field.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2022-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43766218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Jinkui Shenqi and Wuzi Yanzong pill on sperm motility and sperm DNA fragmentation rate in asthenospermia","authors":"Liangliang Xu, Chunhong Xiao, H-Y Cao, Jianfen Yuan, Z. Wu","doi":"10.14393/bj-v38n0a2022-54959","DOIUrl":"https://doi.org/10.14393/bj-v38n0a2022-54959","url":null,"abstract":"This study was designed to compare the effects of Jinkui Shenqi and Wuzi Yanzong pill on sperm motility and sperm DNA fragmentation rate in patients with asthenospermia. 130 cases were randomly divided into an observation and control group (n=65). The control group was treated with the Wuzi Yanzong pill while the observation group with the Jinkui Shenqi pill. The sperm motility parameters rate (PR), semen concentration, sperm motility, DFI and α-glucosidase, fructose, seminal plasma zinc (Zn), acid phosphatase (ACP) in seminal plasma biochemistry and other indexes of were observed. The biochemical indexes of seminal plasma of α-glucosidase, fructose, Zn, ACP in two groups were significantly (p<0.05) improved after treatment. Compared with the control group, the indexes of the observation group improved more obviously after treatment. Pearson correlation analysis of DFI and PR indexes in 130 patients before treatment showed that sperm DFI and PR percentage were negatively correlated in asthenospermia patients (r =-0.572, P<0.05). There was no significant difference in DFI, semen concentration, PR, and sperm motility between the two groups before treatment. The DFI, semen concentration, PR and sperm viability of the two groups showed a tendency to improve after treatment, and the effect of the observation group was less significant than that of the control group (p<0.05). Two groups of patients have completed treatment successfully, no adverse events occurred during treatment. Jinkui Shenqi pill can effectively treat asthenospermia, which can effectively improve the effect of sperm motility in patients. It has less adverse reactions, safe and reliable, and is worthy of promotion.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2022-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45623381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}