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Insecticidal potential of organic extracts of Calotropis procera to Spodoptera frugiperda 纹夜蛾有机提取物对草地贪夜蛾的杀虫潜力
IF 0.5 4区 农林科学
Bioscience Journal Pub Date : 2023-04-06 DOI: 10.14393/bj-v39n0a2023-63699
{"title":"Insecticidal potential of organic extracts of Calotropis procera to Spodoptera frugiperda","authors":"","doi":"10.14393/bj-v39n0a2023-63699","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-63699","url":null,"abstract":"This study evaluated the toxic effects of organic extracts of Calotropis procera leaves on the survival, development, and reproduction of Spodoptera frugiperda. Solutions of crude methanol extract and hexane and methanol fractions of C. procera leaves were added at 1.15% and 2.14% concentrations to the artificial diet of S. frugiperda. The mortality and duration of larval and pupal phases, weights of female and male pupae, deformations of pupae and adults, the reduction of adults able to reproduce, pre-oviposition and oviposition periods, the number of postures per female, and the fecundity and fertility of S. frugiperda females were also evaluated. The extracts harmed the survival, development, and reproduction of S. frugiperda. The ingestion of extracts and fractions by caterpillars affected adults by decreasing the oviposition period, the number of postures, fecundity, and fertility. The crude MeOH extract at a 2.14% concentration harmed the evaluated parameters of the insect, except for pupal mortality, female pupae weight, and pre-oviposition period. The MeOH fraction at 2.14% caused a 50.0% mortality of caterpillars and 16.0% deformation in pupae and 33.0% in adults, reducing by 72.0% the population able to reproduce. The MeOH fraction at the 2.14% concentration caused 25.0% and 38.0% of pupal mortality and deformation, respectively. Calotropis procera has promising insecticidal properties for a biological insecticide, a convenient and sustainable strategy for protecting plants against S. frugiperda.\u0000 ","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41591834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bioclimatic zoning for quails in the rain season in the state of Paraíba, Brazil 巴西帕拉伊巴州雨季鹌鹑的生物气候区划
IF 0.5 4区 农林科学
Bioscience Journal Pub Date : 2023-04-06 DOI: 10.14393/bj-v39n0a2023-66613
V. D. Silva, R. S. Nascimento, J. P. L. Neto, J. Miranda, F. F. M. Lopes, D. A. Furtado
{"title":"Bioclimatic zoning for quails in the rain season in the state of Paraíba, Brazil","authors":"V. D. Silva, R. S. Nascimento, J. P. L. Neto, J. Miranda, F. F. M. Lopes, D. A. Furtado","doi":"10.14393/bj-v39n0a2023-66613","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-66613","url":null,"abstract":"This work aimed to perform bioclimatic zoning based on the temperature and humidity index (THI) for quails from the 3rd to the 5th week of life during the rainy season in the state of Paraíba, Brazil, in order to identify the most suitable mesoregions among the Zona da Mata, Agreste, Borborema and Sertão for the breeding of these birds and to propose mitigating measures for the thermal comfort of the animals. The monthly air temperature and relative humidity data for calculating the THI were obtained from conventional meteorological stations of the National Meteorological Institute of the Brazilian federal government from 1961 to 2015, in turn making maps with the spatial distribution of the index. The index ranged from 71 to 76 in the various mesoregions of Paraíba, with Agreste being the best mesoregion for breeding quails from the 3rd to the 5th week of life, thus providing a thermal comfort zone for birds and only requiring a few corrective measures in their facilities, when necessary, followed by the Sertão, Zona da Mata and Borborema mesoregions.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49579930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Physiological and morphological responses of two beans common genotype to water stress at different phenological stages 两种普通基因型大豆不同物候期对水分胁迫的生理形态响应
IF 0.5 4区 农林科学
Bioscience Journal Pub Date : 2023-04-06 DOI: 10.14393/bj-v39n0a2023-59855
A. Fogaça, A. D. Castro, E. A. Barbosa
{"title":"Physiological and morphological responses of two beans common genotype to water stress at different phenological stages","authors":"A. Fogaça, A. D. Castro, E. A. Barbosa","doi":"10.14393/bj-v39n0a2023-59855","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-59855","url":null,"abstract":"Comprehension of the bean responses of beans common under to water deficit is an important tool in agricultural planning, like sowing time, and deficit irrigation management strategies. The study aimed to understand the morpho-physiological responses and yield attributes of two common bean genotypes submitted to water stress at different phenological stages. The study was carried out in a greenhouse, in randomized block scheme with five repetitions. To achieve the objectives deficit irrigation of 25% of crop evapotranspiration was practiced during vegetative (DI-V), flowering (DI-F), and pod filling (DI-PF) stages. A non-deficit irrigated (NDI) and deficit irrigated through vegetative to pod filling stages (DI-VP) treatments were added for comparison. The following morphophysiological responses and yield attributes were evaluated: net assimilation of CO2, stomatal conductance, and leaf transpiration, chlorophyll index, number of trifoliate leaves, chlorophyll index, leaf area, number of grains per plant, number of grains per pod, number of pods per plant, the mass of thousand grains, harvest index, and water use efficiency. The beans genotype under DI-V exhibited acclimation, observed by the relative increment with NDI of 195%, 759%, and 231% of net assimilation of CO2, stomatal conductance, and leaf transpiration, respectively. Plants under treatment DI-PF experienced dis-stress and plastic responses as leaf losses and exhaustion of gas exchanges. Treatment DI-V received 11% less water than NDI and exhibited equal yield, resulting in higher water use efficiency. Yield attributes correlations indicated that yield penalty might be related to pods abortion, which not occurred to plants under DI-V.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42686706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lawsuits after dermal filler injection registered in the state Court of São Paulo 在圣保罗州法院登记的真皮填充物注射后的诉讼
IF 0.5 4区 农林科学
Bioscience Journal Pub Date : 2023-04-06 DOI: 10.14393/bj-v39n0a2023-67949
{"title":"Lawsuits after dermal filler injection registered in the state Court of São Paulo","authors":"","doi":"10.14393/bj-v39n0a2023-67949","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-67949","url":null,"abstract":"The search for aesthetic procedures in the health sector have increased in the last years. The increase in the number of procedures brought together a higher number of lawsuits, especially when it comes to the injection of dermal fillers. This study aimed to revisit the jurisprudence of the State Court of São Paulo, Brazil, to investigate the risk factors behind the lawsuits and convictions of health care professionals after the injection of dermal fillers. The sample consisted of 46 lawsuits published between 2000 and 2022 in the State Court of São Paulo, Brazil. Data extracted from each lawsuit were: the year of publication, the reason behind the lawsuit, the defendant (individual or legal entity), sex of the plaintiff, type of dermal filler, whether there was a technical legal/forensic examination of the case and conviction. Most of the lawsuits were published between 2020 and 2022 (n = 26, 56.52%). The presence of intercurrences was the most common reason behind the lawsuits (n = 27, 58.69%). The defendants were mostly health care providers (n = 16, 34.78%), individually, and their combination with legal entities (n = 16, 34.78%), separately (the rest was legal entities alone). Female plaintiffs were predominant (n = 36, 78.26%), as well as the use of hyaluronic acid as dermal filler (n = 35, 76.08%). Technical legal/forensic examination was registered in 34 lawsuits (73.91%). Most of the defendants were convicted (n = 25, 54.34%). The chance of conviction was 3,9 times higher if the case was judged between 2017-2019 compared to 2020-2022 (p = 0.05); and 3,4 times higher if the case was based on intercurrences (p = 0.05). It is estimated that convictions in lawsuits after dermal filler injections might decrease in more recent cases. However, higher chances of conviction might be expected in the presence of intercurrences (e.g. accidents or complications) in the State São Paulo, Brazil.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49288089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Epidemiological analysis of Paramphistomum species in Ruminants in selected Districts of Khyber Pakhtunkhwa (KPK), Pakistan 巴基斯坦开伯尔-普赫图赫瓦(KPK)部分地区反刍动物副钩体的流行病学分析
IF 0.5 4区 农林科学
Bioscience Journal Pub Date : 2023-04-06 DOI: 10.14393/bj-v39n0a2023-66497
N. Rafiq, S. Ayaz, S. Niaz, M. Kamal, Luqman Khan
{"title":"Epidemiological analysis of Paramphistomum species in Ruminants in selected Districts of Khyber Pakhtunkhwa (KPK), Pakistan","authors":"N. Rafiq, S. Ayaz, S. Niaz, M. Kamal, Luqman Khan","doi":"10.14393/bj-v39n0a2023-66497","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-66497","url":null,"abstract":"This epidemiological study was conducted to compare the climatic conditions of four different districts of Khyber Pakhtunkhwa, Pakistan, in terms of the prevalence of parasitic infection Paramphistomum in cows and buffaloes. A total of 2400/cows and buffaloes (200/per month and 50/per district) fecal samples were collected. The samples were examined for Paramphistomum egg contamination by direct microscopic examination and sedimentation methods. The infectivity ratio was 17.5% in cows and 17% in buffaloes; the higher rate of infection was recorded in autumn with 41% in cows and 36% in buffaloes. Whereas the lowest ratio was 6.5% in cows and 7% in buffaloes recorded during spring. It was also noted that a high rate of prevalence was recorded in older animals (higher than 2 years of age) than younger (less than 2 years of age), while gender-wise, it was observed that male animals were more affected than females. Prevalence was higher in cows in Mardan district (19.30%) followed by Nowshera (19.10%) and Swabi (17.80%) districts while lowest in Charsadda district (13.80%) where in buffaloes the higher prevalence was recorded in Swabi (18.80%) followed by Mardan (18.60%) then Charsadda (16.80%) while lowest in Nowshera (13.80%).","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41949145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Anti-tyrosinase, anti-skin pathogenic bacterial, and antioxidant activities and phytochemical constituents of Dracaena cochinchinensis (Lour.) S.C. Chen extract 龙血树提取物的抗酪氨酸酶、抗皮肤致病菌、抗氧化活性和植物化学成分
IF 0.5 4区 农林科学
Bioscience Journal Pub Date : 2023-03-31 DOI: 10.14393/bj-v39n0a2023-66896
Tanyapohn Soingam, Moltira Srithaworn
{"title":"Anti-tyrosinase, anti-skin pathogenic bacterial, and antioxidant activities and phytochemical constituents of Dracaena cochinchinensis (Lour.) S.C. Chen extract","authors":"Tanyapohn Soingam, Moltira Srithaworn","doi":"10.14393/bj-v39n0a2023-66896","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-66896","url":null,"abstract":"Dracaena cochinchinensis (Lour.) S.C. Chen (Chandaeng) is an important traditional medicinal plant used in ancient Thai household remedies. This research focused on investigating the biological properties, including the antibacterial, anti-tyrosinase, antioxidant activities, and phytochemical characteristics of crude Chandaeng extracts. Dried Chandaeng heartwood powder was extracted using ethanol, methanol, and deionized water. The antibacterial activities of the extracts were then tested against skin pathogens, including Cutibacterium acnes (DMST14916), Staphylococcus epidermidis (TISTR518), and Staphylococcus aureus (TISTR321). The ethanolic extract showed antibacterial activity. In a time-kill assay, all bacteria were completely killed after being exposed to it, while the cell membranes were found to have leaked when viewed under a scanning electron microscope. Antioxidant potential was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2¢-azino-bis -3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assays. According to the findings, the crude ethanolic extract of Chandaeng showed the highest level of antioxidant activity. Furthermore, the potential of the extract to treat skin hyperpigmentation by inhibiting tyrosinase, an important melanin synthesis enzyme, was determined and the ethanolic extract was found to be an anti-tyrosinase agent. Finally, the crude ethanolic extract showed the highest total phenolic compound and flavonoid content. In conclusion, crude Chandaeng extract showed significant potential in activity against skin pathogenic bacteria, antioxidant activity, and tyrosinase inhibition. These properties of the extract could be applied to skincare cosmetics.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48542936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Application of the multivariate and univariate analyses to estimate the feed efficiency in beef cattle 多变量和单变量分析在肉牛饲料效率评价中的应用
IF 0.5 4区 农林科学
Bioscience Journal Pub Date : 2023-03-31 DOI: 10.14393/bj-v39n0a2023-67310
T. S. Oliveira, I. N. Silva, E. S. Aniceto, José Ribeiro Meirelles Júnior
{"title":"Application of the multivariate and univariate analyses to estimate the feed efficiency in beef cattle","authors":"T. S. Oliveira, I. N. Silva, E. S. Aniceto, José Ribeiro Meirelles Júnior","doi":"10.14393/bj-v39n0a2023-67310","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-67310","url":null,"abstract":"This study aimed to compare the univariate analyses (relationship between dry matter intake (DMI) and average daily gain (ADG), Kleiber ratio, and residual feed intake) and multivariate analysis (bionutritional index [BNI]) to determine feed efficiency. Were used a total of 160 cattle (individual data) and the analyzed variables were dry matter intake, weight gain, and body weight of the animals. We used five methods to evaluate feed efficiency, the BNI, FE, corrected feeding efficiency (corFE), Kleiber ratio (KR), and residual feed intake (RFI).  Study 1 demonstrated that only the FE (p=0.0472) was significant, although the FE after the transformation of Box-Cox (corFE) (p=0.0642) showed a statistical trend. In studies 2, 3, and 5, we observed that BNI was the best biological efficiency indicator. In the study 4, we observed that the best indicators were FE (0.110; p=0.0281), corFE (0.380; p=0.0429), and RFI (0.465; p=0.0340) for the genders. However, corFE decreased the coefficient of variation in all studies. In conclusion, the use of the Box-Cox transformation is as efficient as the multivariate analysis in discriminating experimental groups (genetic groups, different levels of concentrate in the diet, and genders) concerning the other univariate analyzes.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49314919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Relationships between body mass index, ultrasound measurements and, internal body fat depots in pelibuey sheep 佩利布伊羊体重指数、超声测量与体内脂肪库的关系
IF 0.5 4区 农林科学
Bioscience Journal Pub Date : 2023-03-31 DOI: 10.14393/bj-v39n0a2023-67626
R. Salazar-Cuytun, J. Herrera-Camacho, R. García-Herrera, D. Pozo-Leyva, A. Gurgel, L. Ítavo, G. T. Santos, A. Chay-Canul
{"title":"Relationships between body mass index, ultrasound measurements and, internal body fat depots in pelibuey sheep","authors":"R. Salazar-Cuytun, J. Herrera-Camacho, R. García-Herrera, D. Pozo-Leyva, A. Gurgel, L. Ítavo, G. T. Santos, A. Chay-Canul","doi":"10.14393/bj-v39n0a2023-67626","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-67626","url":null,"abstract":"The objective of this study was to evaluate the relationship between body mass index (BMI), ultrasound measurements (USM), and internal fat (IF) in hair sheep. The BMI and USM were determined 24 hours before laughter in 35 animals finished in intensive fattening. At slaughter, the IF (inner adipose tissue) was dissected, weighed, and grouped as mesenteric, omental, or perirenal fat; these three depots were summed to obtain their total weight. The relationship of the variables was evaluated through Pearson's correlation coefficient (r) using the CORR procedure and by regression models using the REG procedure of SAS ver. 9.3. The IF and BMI presented r= 0.75 (P <.0001), while the IF and USM presented an r that varied from 0.70 to 0.80 (P <.0001). Among the internal fat deposits, the perirenal was the one that presented minor correlation coefficients with BMI (r = 0.55), subcutaneous fat thickness (0.55), and thoracic Longissimus dorsi area) (r = 0.33), and for this, the last correlation was not significant (P>0.05). The regression equation between IF and BMI had a coefficient of determination (r2) of 0.67 (P <.0001). The inclusion of the subcutaneous fat thickness together with the BMI improved the r2 with a range of 0.77. The BMI and the subcutaneous fat thickness could be used to predict the weight of the internal fat in hair sheep.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44405437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Application of limestone-molasses blends in Nile tilapia rearing tanks 石灰石-糖蜜混合物在尼罗罗非鱼养殖池中的应用
IF 0.5 4区 农林科学
Bioscience Journal Pub Date : 2023-03-31 DOI: 10.14393/bj-v39n0a2023-63393
{"title":"Application of limestone-molasses blends in Nile tilapia rearing tanks","authors":"","doi":"10.14393/bj-v39n0a2023-63393","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-63393","url":null,"abstract":"The work aimed at determining the effects of applying different blends between liquid molasses and agricultural limestone on water quality and growth performance of Nile tilapia juveniles, Oreochromis niloticus. The study employed 24 indoor tanks of 100 L and 24 outdoor tanks of 250 L. Four control groups were set up (no product applied, only molasses, only limestone, molasses-limestone blend with 48% limestone but no fish) and two experimental groups (molasses-limestone blends with 32% and 48% molasses), with four replicates each. Over 11 weeks, the water quality was monitored systematically. The 24-h cycle monitoring of temperature, pH, specific conductance, dissolved O2 and total alkalinity were carried out at the 8th week. The blends between molasses and limestone accelerated the dissolution rate of the agricultural limestone in water. The blend containing 48% molasses led to greater increases in water alkalinity and pH in relation to tanks with only limestone applications. However, the molasses-limestone blend applications impaired the Nile tilapia growth performance, especially in the outdoor tanks. While the fish yield was 25.1 g m-3 day-1 in the molasses outdoor tanks, it was equal to 22.8 g m-3 day-1 in the molasses (32%)-limestone units (P<0.05). It has been concluded that the blending between limestone and molasses brings no clear benefits to Nile tilapia’s rearing tanks when compared to the limestone-only tanks.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45089807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Glyphosate-resistant hairy fleabane (Conyza bonariensis) exhibits a larger number of trichomes and altered stomatal density relative to the susceptible counterpart 与易感品种相比,抗草甘膦的多毛白莲(Conyza bonariensis)表现出大量的毛状体和改变的气孔密度
IF 0.5 4区 农林科学
Bioscience Journal Pub Date : 2023-03-31 DOI: 10.14393/bj-v39n0a2023-64343
L. C. Baccin, A. Albrecht, R. M. Pedroso, L. S. Araújo, Mateus Augusto Dotta, R. V. Filho
{"title":"Glyphosate-resistant hairy fleabane (Conyza bonariensis) exhibits a larger number of trichomes and altered stomatal density relative to the susceptible counterpart","authors":"L. C. Baccin, A. Albrecht, R. M. Pedroso, L. S. Araújo, Mateus Augusto Dotta, R. V. Filho","doi":"10.14393/bj-v39n0a2023-64343","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-64343","url":null,"abstract":"Following the adoption of Roundup Ready crops, glyphosate spraying frequency increased, while the use of other herbicide modes of action was neglected. Herbicide-resistant biotypes were reported in three major Conyza species in Brazil, including Conyza bonariensis, increasing growers’ bottom line. Considering that leaf surface structures affect proper herbicide deposition, uptake, and performance, this study aimed to characterize epicuticular surface components in glyphosate-resistant (R) and -susceptible (S) C. bonariensis. Conyza spp. seeds were collected in 36 locations in Brazil, and plants were subjected to resistance screening tests by spraying glyphosate at 720 and 1440 g ae ha-1 (0.5X and 1X the label recommended rate, respectively). For resistance level characterization, C. bonariensis biotypes with contrasting responses were selected for glyphosate dose-response assays. Leaf tissues for epicuticular surface analysis were harvested from newly-obtained R and S biotypes at two growth stages. Histological cuts were made on a leaf area of 25 mm² with a blade. Samples were fixed in Karnowsky solution, gradually changed to 100% ethanol, critical-point dried with CO2, and coated with gold, followed by stomatal and trichome density quantification using scanning electron microscopy. Results indicated a poor control with glyphosate in 33 of 36 Conyza spp. biotypes, and a high (31.5) resistance factor was calculated after dose-response trials. Leaf surface analysis indicated that C. bonariensis leaves are amphistomatic and exhibit tectorial trichomes. A higher number of trichomes and altered stomatal density (number.mm²) were quantified in R compared to the S counterpart, potentially reducing glyphosate uptake and effectiveness.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42825146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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