The open pathology journal最新文献_第5页

Recent Thymic Emigrants Do Not Account for the Increased Number of TCells Seen in the Lungs of Stable Chronic Obstructive Pulmonary Disease 近期的胸腺移植物不能解释稳定型慢性阻塞性肺疾病患者肺中t细胞数量增加的原因

The open pathology journal Pub Date : 2008-09-18 DOI: 10.2174/1874375700802010102

G. Caramori, Kazuhiro Ito, E. Jazrawi, P. Casolari, K. Chung, P. Barnes, I. Adcock, A. Papi

引用次数: 0

Correlation Between mRNA Detected by Microarrays and qRT-PCR and Protein Detected by Immunohistochemistry of Cyclins in Tumour Tissue from Colonic Adenocarcinomas 微阵列和qRT-PCR检测细胞周期蛋白mRNA与免疫组化检测蛋白的相关性

The open pathology journal Pub Date : 2008-09-18 DOI: 10.2174/1874375700802010096

K. Jonsdottir, R. Størkson, A. Krog, I. Bukholm

{"title":"Correlation Between mRNA Detected by Microarrays and qRT-PCR and Protein Detected by Immunohistochemistry of Cyclins in Tumour Tissue from Colonic Adenocarcinomas","authors":"K. Jonsdottir, R. Størkson, A. Krog, I. Bukholm","doi":"10.2174/1874375700802010096","DOIUrl":"https://doi.org/10.2174/1874375700802010096","url":null,"abstract":"There are several studies evaluating the prognostic value of overexpression of cyclin proteins, detected by im- munohistochemistry. Overexpression detected by microarray and real-time PCR techniques is now applied in analyzing the prognostic value of different genes on patient outcome. In this study we wanted to evaluate the correlation between these three methods for detection of cyclins (A2, B1, D1, D3, E and H) overexpression, as well as correlation between overexpression and gene amplification of the cyclin genes. We used both normal mucosa and tumour tissue obtained from 22 patients at the time of surgery. None of the cyclins were defined to be amplified at the gene level. qRT-PCR and cDNA microarray results are comparable, but no correlation between gene expression and protein expression, measured by im- munohistochemistry was observed. This may indicate that immunohistochemistry detects some other parameters than gene expression analysis measured by cDNA microarray and qRT-PCR. It is of interest to evaluate which of the three methods may give the most important information about patient prognosis.","PeriodicalId":89507,"journal":{"name":"The open pathology journal","volume":"8 1","pages":"96-101"},"PeriodicalIF":0.0,"publicationDate":"2008-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79601719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Type 2 Diabetes: Local Inflammation and Direct Effect of Bacterial Toxic Components 2型糖尿病:局部炎症和细菌毒性成分的直接作用

The open pathology journal Pub Date : 2008-09-12 DOI: 10.2174/1874375700802010086

J. Miklossy, R. Martins, N. Darbinian, K. Khalili, P. Mcgeer

{"title":"Type 2 Diabetes: Local Inflammation and Direct Effect of Bacterial Toxic Components","authors":"J. Miklossy, R. Martins, N. Darbinian, K. Khalili, P. Mcgeer","doi":"10.2174/1874375700802010086","DOIUrl":"https://doi.org/10.2174/1874375700802010086","url":null,"abstract":"Objectives: It has been known for almost a century that amyloidosis is frequently associated with chronic bacte- rial infection. Islet amyloid deposit is characteristic of type 2 diabetes. Periodontal disease, which is predominantly caused by several Gram negative bacteria, is a risk factor for type 2 diabetes. The goal of the study was to explore whether bacte- ria or their toxic components may play a role in type 2 diabetes. Material & Methods: The pancreas in 22 autopsy cases was analyzed for the presence of lipopolysaccharide (LPS), bacte- rial peptidoglycan (BPG) and local inflammatory processes. Ten of the cases had clinically diagnosed type 2 diabetes, and 12 were age matched controls. Results: The results of an immunohistochemical analysis showed the presence of LPS and BPG in association with islet amyloid deposits in all the 10 diabetic cases as well as in 3 controls with clinically silent amyloid deposits. Chlamydia pneumoniae and Helicobacter pylori specific antigens were detected in the affected islets in a subset of diabetic patients. Clumps of HLA-DR positive activated macrophages, abundant immunoreactivity to the activated complement compo- nents C3d, C4d and C5b-9, the terminal attack complex, and a moderate numbers of T4 and particularly of T8 lympho- cytes were present in the pancreas of all diabetic cases. Conclusions: These results suggest that bacteria or their slowly degradable remnants may initiate and sustain chronic in- flammation in the pancreas and therefore play a role in the pathogenesis of type 2 diabetes. They also indicate that local immune responses, including activation of the classical complement pathway are important in the pathogenesis of type 2 diabetes. There may also be some involvement of the adaptive immune system. Further investigations are essential since a parallel use of antibacterial and anti-inflammatory drugs may prevent or slow down the disease progression.","PeriodicalId":89507,"journal":{"name":"The open pathology journal","volume":"18 1","pages":"86-95"},"PeriodicalIF":0.0,"publicationDate":"2008-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81299340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Quantitative Flow Cytometry Immunophenotypic Data in Myelodysplastic Syndromes (MDS) 骨髓增生异常综合征(MDS)的定量流式细胞术免疫表型数据

The open pathology journal Pub Date : 2008-07-09 DOI: 10.2174/1874375700802010078

H. Nishino, April A. Ewton, A. Euton, Y. Zu, A.Ponce de León, K. Baker, G. Carrum, S. Chakraborty, L. Rice, C. Chang

{"title":"Quantitative Flow Cytometry Immunophenotypic Data in Myelodysplastic Syndromes (MDS)","authors":"H. Nishino, April A. Ewton, A. Euton, Y. Zu, A.Ponce de León, K. Baker, G. Carrum, S. Chakraborty, L. Rice, C. Chang","doi":"10.2174/1874375700802010078","DOIUrl":"https://doi.org/10.2174/1874375700802010078","url":null,"abstract":"Background: Recent studies using qualitative analysis of flow cytometry data have demonstrated various im- munophenotypic abnormalities associated with myelodysplastic syndromes (MDS). However, there are limited reports as- sessing the ability of quantitative immunophenotypic analysis to discriminate MDS from other cytopenic conditions. Design: Using flow cytometry, we studied 37 bone marrow specimens from 23 patients with MDS and 14 cytopenic pa- tients with non-clonal hematologic disorders. Samples were analyzed quantitatively for percentages of T-cells, B-cells, NK cells, granulocytes, monocytes, blasts, erythroid precursors, and plasma cells; CD4:CD8 ratio; % granulocyte subsets; % CD56+ monocytes; and % erythroid precursor subsets. Results: Quantitative analysis of immunophenotypic data in MDS patients compared to controls showed decreases in total granulocytes (p=0.037) and more mature subsets of CD11b + CD16 bright granulocytes (p=0.0046) and CD10 + granulocytes (p=0.022). MDS patients also showed a trending increase in subset percentage of CD56+monocytes (p=0.056). Using re- ceiver operating characteristic (ROC) analysis, cut-off values for these parameters favoring a diagnosis of MDS were identified as follows: total granulocytes 10%. Subsequently, a scoring system was proposed whereby a score of one was assigned for the presence of each quantitative abnormality. Using this system on the original study population, the pres- ence of at least two abnormalities (score￻ 2) revealed optimal sensitivity (69.6%) and specificity (71.4%) for a diagnosis of MDS. Conclusion: These findings suggest that quantitative analysis of immunophenotypic data can be complementary to quali- tative interpretation. These data may be useful for distinguishing MDS from non-clonal cytopenic disorders and warrant prospective study in additional MDS patients.","PeriodicalId":89507,"journal":{"name":"The open pathology journal","volume":"14 1","pages":"78-85"},"PeriodicalIF":0.0,"publicationDate":"2008-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77102913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microsurgical Extrahepatic Cholestasis in the Rat: A Histopathological Liver Study 大鼠肝外胆汁淤积的显微外科研究:肝脏组织病理学研究

The open pathology journal Pub Date : 2008-07-08 DOI: 10.2174/1874375700802010071

M. Aller, Luis Ortega, F. Sánchez-Patán, R. Anchuelo, Arturo Cruz, M. Losada, J. Arias

{"title":"Microsurgical Extrahepatic Cholestasis in the Rat: A Histopathological Liver Study","authors":"M. Aller, Luis Ortega, F. Sánchez-Patán, R. Anchuelo, Arturo Cruz, M. Losada, J. Arias","doi":"10.2174/1874375700802010071","DOIUrl":"https://doi.org/10.2174/1874375700802010071","url":null,"abstract":"Fibrosis, bile duct proliferation and mast cell hyperplasia characterize extrahepatic cholestasis. We studied these liver alterations in a new microsurgical model of extrahepatic cholestasis in the rat. Male Wistar rats: Sham-operated (n=9) and Microsurgical Cholestasis (n=10). After 4 weeks, a liver morphometric study was carried out using an image analysis system to assess bile proliferation and the fibrosis. The liver expression of alpha-smooth muscle actin was assayed by an immunohistochemical technique and mast cells were also counted. Cholestatic-rats presented portal hypertension, portosystemic circulation, and increased (p=0.0001) plasma bilirubin, alkaline phosphatase, Aspartateaminotransferase, Alanine-aminotransferase, and Lactate-dehydrogenase. Plasma levels of Albumin decreased (p=0.001). Cholestatic-rats showed intense biliary duct proliferation (p=0.0001) and fibrosis (p=0.0001). Mast cells accumulate (p=0.0001) around proliferating bile ducts and fibrous septa. The microsurgical cholestasis in the rat induces fibrosis and hyperplasia of bile ducts and mast cells. Mast cell liver infiltration could be an etiopathogenic remodeling factor in experimental cholestasis.","PeriodicalId":89507,"journal":{"name":"The open pathology journal","volume":"28 1","pages":"71-77"},"PeriodicalIF":0.0,"publicationDate":"2008-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73897921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

The Role of Systems Biology in Predictive Health and Personalized Medicine 系统生物学在预测性健康和个性化医疗中的作用

The open pathology journal Pub Date : 2008-06-26 DOI: 10.2174/1874375700802010068

E. Voit, K. Brigham

引用次数: 24

The Gene Variant MLH1*D132H is Not Associated with an Increased Risk for Colorectal Adenomas in a Suburban United States Jewish Population 基因变异MLH1*D132H与美国郊区犹太人结直肠腺瘤风险增加无关

The open pathology journal Pub Date : 2008-06-05 DOI: 10.2174/1874375700802010063

P. Zauber, M. Sabbath‐Solitare, S. Marotta, W. Foulkes, T. Bishop

引用次数: 0

Senescence as a Target for Cancer Therapy 衰老是癌症治疗的靶点

The open pathology journal Pub Date : 2008-04-29 DOI: 10.2174/1874375700802010057

H. Kondoh, T. Maruyama, M. Lleonart

引用次数: 3

TST-Negative Individuals Do Not Lack Anti-Mycobacterial Responsiveness tst阴性个体不缺乏抗分枝杆菌反应性

The open pathology journal Pub Date : 2008-04-29 DOI: 10.2174/1874375700802010053

A. Godel, P. Peyron, Marie-Pierre Puissegur, Puissegur Botanch, B. Payre, S. Khatibi, P. Massip, B. Marchou, F. Altare

引用次数: 0

Extraction of Phosphorylated Proteins from Formalin-Fixed Cancer Cells and Tissues 福尔马林固定癌细胞和组织中磷酸化蛋白的提取

The open pathology journal Pub Date : 2008-04-21 DOI: 10.2174/1874375700802010046

K. Becker, H. Mack, C. Schott, S. Hipp, A. Rappl, G. Piontek, H. Höfler

{"title":"Extraction of Phosphorylated Proteins from Formalin-Fixed Cancer Cells and Tissues","authors":"K. Becker, H. Mack, C. Schott, S. Hipp, A. Rappl, G. Piontek, H. Höfler","doi":"10.2174/1874375700802010046","DOIUrl":"https://doi.org/10.2174/1874375700802010046","url":null,"abstract":"Deregulated signal pathways are promising targets for cancer therapy as they can drive tumour growth, sur- vival, invasion, and metastasis. In most hospitals around the world, histopathological cancer diagnosis is based upon for- malin-fixed and paraffin-embedded (FFPE) tissues. In this study, we evaluated whether phosphorylated signaling proteins can be extracted from cells and tissues after formalin fixation. Three epidermal growth factor (EGF) receptor-positive cell lines were stimulated with EGF for various time points and then either fixed with formalin or left unfixed before protein extraction. Using Western blot, we were able to detect all phosphorylated proteins analyzed, including activated (phos- phorylated) extracellular signal-regulated kinase (ERK) 1 and 2. There were no differences between formalin-fixed and unfixed cells. Because ERK1/2 kinases are involved in HER2-mediated signal transduction, we applied our methodology to study ERK1/2 and phospho-ERK1/2 in 20 primary breast cancers using protein lysate microarray technology. We found a statistically significant correlation between HER2 status and ERK1/2 expression; however, activation (phosphory- lation) of ERK1/2 was independent of HER2 expression. Our study demonstrates the successful extraction of phosphory- lated proteins from FFPE tissue samples. Monitoring phosphorylated signaling proteins in FFPE tissues, as applied here, may allow us in the near future to infer the activity levels of proteins in particular pathways as starting point for the design of individual combination therapy regimens without changing the routine clinical workflow for tissue analysis.","PeriodicalId":89507,"journal":{"name":"The open pathology journal","volume":"37 1","pages":"46-52"},"PeriodicalIF":0.0,"publicationDate":"2008-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81526959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18