Bioresource Technology Reports最新文献_第3页

Increased activity under acidic conditions and improved thermostability of Aspergillus niger phytase A 提高了黑曲霉植酸酶A在酸性条件下的活性和热稳定性

Bioresource Technology Reports Pub Date : 2025-06-01 DOI: 10.1016/j.biteb.2025.102161

Anna Dotsenko , Aleksandra Rozhkova , Ivan Zorov , Pavel Volkov , Arkady Sinitsyn

{"title":"Increased activity under acidic conditions and improved thermostability of Aspergillus niger phytase A","authors":"Anna Dotsenko , Aleksandra Rozhkova , Ivan Zorov , Pavel Volkov , Arkady Sinitsyn","doi":"10.1016/j.biteb.2025.102161","DOIUrl":"10.1016/j.biteb.2025.102161","url":null,"abstract":"<div><div>Phytases are high-demand enzymes in the production of feed additives for broilers. Both high enzyme activity under acidic pH and high thermostability are required. The medium in the crop and proventriculus/gizzard of broilers is acidic, while the feed pelleting process includes heat treatment. Improvement in both activity and thermostability is a complicated task because an increase in the rigidity of the protein molecule that accompanies the increase in the thermostability generally decreases the activity, and vice versa. In the study, both activity and thermostability of <em>Aspergillus niger</em> phytase A were improved through rational design and site-directed mutagenesis. The activity at pH 2.5 and thermostability at 90 °C were increased by 1.6 and 1.4 times, respectively. Molecular dynamics modeling of the enzyme-substrate complex showed better binding of the substrate that correlated with a 2.3-fold improvement in <em>K</em><sub><em>m</em></sub>, while a reduction in fluctuation at 90 °C correlated with the improved thermostability.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"30 ","pages":"Article 102161"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144184634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of lime impregnation coupled with twin-screw extrusion pretreatment to improve biomethane production from corn stover 优化石灰浸渍-双螺杆挤压预处理提高玉米秸秆生物甲烷产量

Bioresource Technology Reports Pub Date : 2025-05-26 DOI: 10.1016/j.biteb.2025.102158

Arthur Chevalier , Philippe Evon , Florian Monlau , Virginie Vandenbossche , Cecilia Sambusiti

{"title":"Optimization of lime impregnation coupled with twin-screw extrusion pretreatment to improve biomethane production from corn stover","authors":"Arthur Chevalier , Philippe Evon , Florian Monlau , Virginie Vandenbossche , Cecilia Sambusiti","doi":"10.1016/j.biteb.2025.102158","DOIUrl":"10.1016/j.biteb.2025.102158","url":null,"abstract":"<div><div>This study investigated the impact of lime addition coupled with twin-screw extrusion pretreatment to enhance biomethane production from corn stover at laboratory-scale (<em>i.e.</em>, inlet flows of 5 kg FM/h for extrusion). A dry matter optimization phase was firstly conducted to identify the ideal solid loading for lime impregnation within the extruder technical limits. It resulted in an optimal dry matter content of 30gDM/100gFM for a biochemical methane potential (BMP) increase of +18 % compared to raw CS. Then, a comparison between lime impregnation upstream or downstream extrusion was investigated. No statistical differences between lime impregnation upstream or downstream extrusion was evidenced for biochemical methane potential with +22 % and +27 % BMP increases, respectively. However, higher lignocellulosic depolymerization in the case of lime impregnation downstream extrusion (up to 17 %) was obtained and resulted in a higher increase of the specific biomethane production rate (up to +93 %). Consequently, both methods are equivalent for incrementing BMP, but downstream impregnation is superior for improving the production rate. Ultimately, scale-up semi-continuous AD trials should be investigated to provide a more realistic assessment of industrial biomethane production.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"30 ","pages":"Article 102158"},"PeriodicalIF":0.0,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144169755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Patching-up of major influencing factors of Rhizophagus irregularis cultivation for industrial production of consistent spore density through cascade statistical optimization 用级联统计优化方法补齐不规则根噬菌工业化生产中孢子密度一致栽培的主要影响因素

Bioresource Technology Reports Pub Date : 2025-05-26 DOI: 10.1016/j.biteb.2025.102160

Jubair Al Rashid , Md Abuhena , Jingjing Wang , Md. Faisal Azim , Zhiyong Huang

{"title":"Patching-up of major influencing factors of Rhizophagus irregularis cultivation for industrial production of consistent spore density through cascade statistical optimization","authors":"Jubair Al Rashid , Md Abuhena , Jingjing Wang , Md. Faisal Azim , Zhiyong Huang","doi":"10.1016/j.biteb.2025.102160","DOIUrl":"10.1016/j.biteb.2025.102160","url":null,"abstract":"<div><div>Arbuscular mycorrhizal fungi (AMF) play a crucial role as a climate-change solution, aiding nutrient uptake, interplant communication, and stress mitigation. However, its industrial-scale production remains limited. This study aimed to optimize production of <em>Rhizophagus irregularis</em> (Apex-MYK-01) in carrot root organ cultures using a cascading approach to achieve consistent spore yields. The first phase identified ideal conditions: 2 × 2 cm inoculum size, 100 mL media, and using 90-day-old inoculum in box-shaped vessels. The second phase revealed four critical factors (p < 0.05) influencing output. Final optimization of those four parameters via central composite design pinpointed optimal values: 80-day incubation, 1.25 cm media height, 0.875 % sugar, and 2.75 mg KH₂PO₄. When tested at industrial scale, these conditions boosted spore production by 16.8 % (2213 ± 11 vs. lab-scale 1895 ± 15 spores/g) while maintaining high root colonization rates (82 ± 11 %). This cascading strategy demonstrates a viable path to standardized, large-scale AMF production.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"30 ","pages":"Article 102160"},"PeriodicalIF":0.0,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144169765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic effect of Trichoderma viride enriched rotary drum compost and vermicompost extracts against fungal phytopathogens 富含绿木霉的转鼓堆肥和蚯蚓堆肥提取物对真菌植物病原体的协同作用

Bioresource Technology Reports Pub Date : 2025-05-22 DOI: 10.1016/j.biteb.2025.102156

Jyoti, Meena Khwairakpam

{"title":"Synergistic effect of Trichoderma viride enriched rotary drum compost and vermicompost extracts against fungal phytopathogens","authors":"Jyoti, Meena Khwairakpam","doi":"10.1016/j.biteb.2025.102156","DOIUrl":"10.1016/j.biteb.2025.102156","url":null,"abstract":"<div><div>Compost extracts offer sustainable alternatives to agrochemicals for plant disease management. This study assessed the bioefficacy of rotary drum compost (RDC) and vermicompost (VC) extracts, with and without enrichment by <em>Trichoderma viride</em>, against fungal phytopathogens. Molecular identification of fungal phytopathogens confirmed the isolates belongs to <em>Aspergillus</em> spp. Compost extracts were prepared using three different extraction treatments. In vitro dual culture assays revealed highest mycelial inhibition (up to 90.52 %) in enriched compost extracts. Enrichment with <em>T.viride</em> significantly increased mycelial inhibition, with compost type and extraction method having a significant effect than pathogen species<em>.</em> In vivo assays in <em>Vigna radiata</em> seedlings further confirmed enhanced disease suppression and seedling growth promotion. The enriched vermicompost extracts exhibited the highest root and shoot lengths, germination index (98 %), and lowest disease incidence (≤10 %). These findings demonstrate the potential of <em>T. viride</em>-enriched compost extracts as effective, eco-friendly bioproducts for integrated disease management and plant growth promotion.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"30 ","pages":"Article 102156"},"PeriodicalIF":0.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144169756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review on algal oils as a sustainable source of essential omega-3 fatty acids 藻类油作为必需omega-3脂肪酸的可持续来源的综述

Bioresource Technology Reports Pub Date : 2025-05-20 DOI: 10.1016/j.biteb.2025.102154

Plash Kunj , Sudarshan Sahu , Gursharan Singh , Shailendra Kumar Arya

{"title":"A review on algal oils as a sustainable source of essential omega-3 fatty acids","authors":"Plash Kunj , Sudarshan Sahu , Gursharan Singh , Shailendra Kumar Arya","doi":"10.1016/j.biteb.2025.102154","DOIUrl":"10.1016/j.biteb.2025.102154","url":null,"abstract":"<div><div>Omega-3 fatty acids are vital for human health, yet traditional sources such as fish oils face challenges including overfishing, environmental concerns, and contaminants. This review explores algal oils as a sustainable and efficient alternative for omega-3 production. Microalgae, cultivated under controlled conditions, offer a vegan, eco-friendly solution for producing high-quality DHA and EPA. Advances in biotechnological methods, such as genetic engineering and hydrothermal liquefaction, have optimized algal oil yields, enhancing its scalability and affordability. The review highlights innovative extraction techniques, including ultrasonication and enzymatic methods, which preserve oil quality while minimizing environmental impact. Furthermore, the integration of algal omega-3 s into nutraceuticals, aquaculture, and functional foods is transforming health and environmental sustainability. This work underscores the potential of algal omega-3 s in addressing global dietary needs while promoting sustainable practices. By bridging scientific innovation and environmental awareness, algal oils present a compelling path forward for human health and ecological preservation.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"30 ","pages":"Article 102154"},"PeriodicalIF":0.0,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144105774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioelectrochemical treatment of emerging contaminants and molecular characterization of transformation products in landfill leachate 垃圾渗滤液中新兴污染物的生物电化学处理及转化产物的分子表征

Bioresource Technology Reports Pub Date : 2025-05-19 DOI: 10.1016/j.biteb.2025.102153

Sushma K. Varma , Rajesh Singh

{"title":"Bioelectrochemical treatment of emerging contaminants and molecular characterization of transformation products in landfill leachate","authors":"Sushma K. Varma , Rajesh Singh","doi":"10.1016/j.biteb.2025.102153","DOIUrl":"10.1016/j.biteb.2025.102153","url":null,"abstract":"<div><div>The increasing environmental risk posed by emerging contaminants (ECs) in landfill leachate (LL) necessitates advanced monitoring and treatment strategies. This study aimed to identify and characterize approximately 100 ECs—including pharmaceuticals, pesticides, and industrial chemicals—and their transformation products (TPs) in LL and associated solid waste using suspect and non-target screening via LC-QTOF-MS. The treatment was conducted using a sulfate-reducing bacteria-based bio-electrochemical system (SRB-BES). Results revealed pharmaceuticals and diagnostic agents as the dominant ECs (27.27 %), followed by pesticides and herbicides (16.16 %). The integrated SRB-BES system effectively degraded several toxic compounds into less harmful TPs, such as the conversion of hydrocortisone acetate into cortisone. The study provides a comprehensive workflow for TP identification and demonstrates the SRB-BES system's potential for mitigating the ecological impact of legacy pollutants in mature landfills. These findings support the development of sustainable waste management solutions and improved contaminant surveillance frameworks.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"30 ","pages":"Article 102153"},"PeriodicalIF":0.0,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144123852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of substrate concentration on single-cell protein production from green biorefinery residual juice 底物浓度对绿色生物炼制残汁单细胞蛋白产量的影响

Bioresource Technology Reports Pub Date : 2025-05-18 DOI: 10.1016/j.biteb.2025.102151

Mathias Frydensberg Sørensen, Thalles Allan Andrade, Natália Hachow Motta dos Passos, Morten Ambye-Jensen

{"title":"Influence of substrate concentration on single-cell protein production from green biorefinery residual juice","authors":"Mathias Frydensberg Sørensen, Thalles Allan Andrade, Natália Hachow Motta dos Passos, Morten Ambye-Jensen","doi":"10.1016/j.biteb.2025.102151","DOIUrl":"10.1016/j.biteb.2025.102151","url":null,"abstract":"<div><div>Producing single-cell protein (SCP) using an underutilized side-stream from green biorefineries, brown juice (BJ), addresses global food security and environmental challenges. In this work, BJ from a green biorefinery was investigated as a fermentation medium for cultivating the SCP yeast <em>Cyberlindnera jadinii</em>. Effects of C/N ratio, BJ concentration, and maceration methods on yeast growth and yields were analyzed. Results showed <em>C. jadinii</em> could not utilize pentoses within 24 h, reducing protein yield. Adding nitrogen increased protein and biomass yields, with a maximum protein yield of 0.37 g g<sup>−1</sup> sugar at a C/N ratio of 5.3 g g<sup>−1</sup>. Concentrating BJ extended the lag phase but maintained protein yield, reaching a maximum crude protein concentration of 11.9 g L<sup>−1</sup>. Both one-stage and multi-stage macerated BJ supported growth, with one-stage maceration achieving slightly higher yields. Adjusting the C/N ratio in concentrated BJ enhanced SCP production, demonstrating its potential as a sustainable fermentation medium.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"30 ","pages":"Article 102151"},"PeriodicalIF":0.0,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactic acid fermentation of sugars produced by fast pyrolysis of cellulose and effects of by-products on fermentation 纤维素快速热解产糖的乳酸发酵及副产物对发酵的影响

Bioresource Technology Reports Pub Date : 2025-05-18 DOI: 10.1016/j.biteb.2025.102152

Yasuko Maruichi, Takashi Nomura, Eiji Minami, Haruo Kawamoto

{"title":"Lactic acid fermentation of sugars produced by fast pyrolysis of cellulose and effects of by-products on fermentation","authors":"Yasuko Maruichi, Takashi Nomura, Eiji Minami, Haruo Kawamoto","doi":"10.1016/j.biteb.2025.102152","DOIUrl":"10.1016/j.biteb.2025.102152","url":null,"abstract":"<div><div>Saccharification of cellulose is a crucial step in the conversion of biomass into bio-based chemicals and fuels. Among various saccharification methods, fast pyrolysis of cellulose was conducted, resulting in the production of bio-oil containing levoglucosan as the major component, along with various by-products such as glycolaldehyde, glyoxal, methylglyoxal, furfural, 5-hydroxymethylfurfural, acetic acid, formic acid, and formaldehyde. The levoglucosan in the bio-oil was hydrolyzed into glucose and used as a sugar solution for lactic acid fermentation. However, the presence and concentrations of these by-products significantly affected the fermentation process. The inhibitory effects of the by-products were ranked in the order of aldehydes > organic acids > furans, as these compounds interfere with the microbial activity necessary for efficient lactic acid production. To address this issue, an ion-exchange resin containing amino groups was utilized to purify the sugar solution by selectively removing the aldehyde-containing compounds, which comprise the majority of inhibitory substances. This purification enabled successful lactic acid fermentation, even at sugar concentrations that were previously inhibitory. These findings highlight the potential of bio-oil derived from cellulose pyrolysis as a sustainable feedstock for bioprocessing, provided that effective purification strategies are implemented.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"30 ","pages":"Article 102152"},"PeriodicalIF":0.0,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144125370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of operational parameters on the yield of biochar, bio-oil, and pyrolytic gas in lignocellulosic biomass pyrolysis: A systematic review 操作参数对木质纤维素生物质热解中生物炭、生物油和热解气体产量的影响：系统综述

Bioresource Technology Reports Pub Date : 2025-05-17 DOI: 10.1016/j.biteb.2025.102155

Ivaldir José Tamagno Junior , José Luiz Francisco Alves , Claiton Zanini Brusamarello , Michele Di Domenico

{"title":"Impact of operational parameters on the yield of biochar, bio-oil, and pyrolytic gas in lignocellulosic biomass pyrolysis: A systematic review","authors":"Ivaldir José Tamagno Junior , José Luiz Francisco Alves , Claiton Zanini Brusamarello , Michele Di Domenico","doi":"10.1016/j.biteb.2025.102155","DOIUrl":"10.1016/j.biteb.2025.102155","url":null,"abstract":"<div><div>Lignocellulosic biomass represents a promising renewable energy source, particularly when converted <em>via</em> pyrolysis, which produces biochar, bio-oil, and gas. This study consolidates current knowledge through a systematic review analyzing the effects of operating parameters on product yields. The structured methodology included three steps: planning, selection/classification, and summary/reporting. Findings indicate that biochar yield is favored by lower temperatures, slower heating rates, shorter residence times, and larger particle sizes. Gas production increases with higher temperatures, faster heating rates, longer times, and smaller particles. Bio-oil yield exhibits a more complex pattern, with optimal production at temperatures near 500 °C, faster heating rates, longer times, and particles below 2.0 mm. Variables such as carrier gas flow, humidity, and reactor type also influence pyrolysis outcomes. Despite identified trends, inconsistencies in experimental results, often due to feedstock variability, highlight the need for more controlled, comparative studies. Addressing these gaps is essential for optimizing pyrolysis for sustainable energy production.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"30 ","pages":"Article 102155"},"PeriodicalIF":0.0,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144105775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Techno-economic and emission impact evaluation of crop residues-biochar system for carbon sequestration 作物残茬-生物炭固碳系统技术经济及排放影响评价

Bioresource Technology Reports Pub Date : 2025-05-17 DOI: 10.1016/j.biteb.2025.102145

Abhijeet Anand , Vivek Kumar , Priyanka Kaushal

引用次数: 0