GM cropsPub Date : 2022-10-21DOI: 10.3390/crops2040029
R. Geneve, Evan W. Janes, S. Kester, D. Hildebrand, Derrick Davis
{"title":"Temperature Limits for Seed Germination in Industrial Hemp (Cannabis sativa L.)","authors":"R. Geneve, Evan W. Janes, S. Kester, D. Hildebrand, Derrick Davis","doi":"10.3390/crops2040029","DOIUrl":"https://doi.org/10.3390/crops2040029","url":null,"abstract":"Industrial hemp (Cannabis sativa L.) as a grain and fiber crop is experiencing a resurgence in North America. Due to governmental prohibition, there has been limited information on regional agronomic production systems including basic information on seed germination. This study was initiated to provide basic information on the relationship between temperature and germination in hemp seed. Germination was measured at constant temperatures ranging from 3 to 42 °C. Cardinal temperatures were determined for two industrial oil crop hemp cultivars (‘Georgina’ and ‘Victoria’). The optimal germination temperature indicated by a high mean germination percentage and rate was between 19 and 30 °C. Optimal (29.6 °C), base (3.4 °C) and ceiling (42.6 °C) temperatures were calculated from a linear regression of the germination rates to reach 50% germination for each temperature. The thermal time for ‘Georgina’ and ‘Victoria’ to reach 50% germination at suboptimal temperatures was 694 and 714 °C h, respectively. The osmotic and solid matrix-primed hemp seeds germinated faster than the untreated seeds, but the final germination percentages were not different. The primed seeds germinated faster at supraoptimal temperatures but did not impact final germination percentages in the thermally inhibited seeds.","PeriodicalId":89376,"journal":{"name":"GM crops","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88134201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
GM cropsPub Date : 2022-10-21DOI: 10.3390/crops2040028
Jiahou Tan, S. Kester, Kai Su, D. Hildebrand, R. Geneve
{"title":"Seed Priming and Pericarp Removal Improve Germination in Low-Germinating Seed Lots of Industrial Hemp","authors":"Jiahou Tan, S. Kester, Kai Su, D. Hildebrand, R. Geneve","doi":"10.3390/crops2040028","DOIUrl":"https://doi.org/10.3390/crops2040028","url":null,"abstract":"Industrial hemp (Cannabis sativa L.) is experiencing a resurgence in North America as an agricultural commodity. Germplasm improvement for locally adapted and stable cannabinoid production is an industry priority. This study used seed priming and pericarp removal to recover seedlings in low-germinating seed lots that could prove important for plant breeding and germplasm conservation. Both seed priming and pericarp removal improved early seed germination, but pericarp removal was more effective in improving overall final germination percentages. On average, pericarp removal improved final germination in low-germinating seed lots by approximately 38% compared to intact seed germination. In seeds with the pericarp removed, the initial germination substrate had an impact on normal seedling development following transplanting: those germinated for 2 to 3 days on rolled towels produced more normal seedlings compared to those started in Petri dishes. There was a dramatic increase in abnormal seedlings produced in the low-germinating seed lot initially germinated in a Petri dish wetted with 6 mL water where nearly 80% never transitioned to normal actively growing seedlings.","PeriodicalId":89376,"journal":{"name":"GM crops","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80267535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
GM cropsPub Date : 2022-10-20DOI: 10.3390/crops2040027
Yasuhito Sakuraba, N. Paek
{"title":"Overexpression of ONAC054 Improves Drought Stress Tolerance and Grain Yield in Rice","authors":"Yasuhito Sakuraba, N. Paek","doi":"10.3390/crops2040027","DOIUrl":"https://doi.org/10.3390/crops2040027","url":null,"abstract":"Drought stress negatively affects plant growth and development, thus reducing plant productivity. Therefore, understanding the molecular mechanisms underlying drought stress responses is essential for crop improvement. The plant-specific NAM/ATAF1,2/CUC2 (NAC) transcription factors play important roles in the drought stress response. Here, we show that rice (Oryza sativa) ONAC054, a membrane-bound NAC transcription factor, is involved in the drought stress response. We found that onac054 mutants were sensitive, whereas ONAC054-overexpressing (ONAC054-OX) plants were tolerant to drought stress. Under drought stress conditions, several genes associated with abscisic acid (ABA) synthesis and signaling were downregulated in onac054 mutants but upregulated in ONAC054-OX plants. Among these genes, the TRANSCRIPTION FACTOR RESPONSIBLE FOR ABA REGULATION 1 (TRAB1), which encodes an ABA-inducible bZIP transcription factor, was directly activated by ONAC054. On the other hand, the expression of ONAC054 was directly activated by several ABA-responsive elements (ABRE)-binding factors (ABFs) in an ABA-dependent manner, indicating that ONAC054 acts as an enhancer of ABA-induced drought stress tolerance. Additionally, the overexpression of ONAC054 in rice greatly improved grain yield under drought stress conditions, indicating that the overexpression of ONAC054 could facilitate the improvement of drought stress tolerance in rice and other crops.","PeriodicalId":89376,"journal":{"name":"GM crops","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80351634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
GM cropsPub Date : 2022-10-06DOI: 10.3390/crops2040026
Matthias Stegner, O. Buchner, Tanja Schäfernolte, Andreas Holzinger, G. Neuner
{"title":"Responses to Ice Formation and Reasons of Frost Injury in Potato Leaves","authors":"Matthias Stegner, O. Buchner, Tanja Schäfernolte, Andreas Holzinger, G. Neuner","doi":"10.3390/crops2040026","DOIUrl":"https://doi.org/10.3390/crops2040026","url":null,"abstract":"Potato leaves are ice-tolerant but are frost-damaged at −3 °C. Freezing occurs in two steps, a first non-destructive freezing event and a second independent lethal event. Localization of ice, and whether cells freeze-dehydrate after the first freezing event remains unknown. The cause of frost damage during the second freezing event lacks experimental evidence. Cytological responses of mesophyll cells were examined during ice formation using cryo-microscopic techniques after high-pressure freeze-fixation and freeze-substitution. CO2 gas exchange on frozen leaves revealed functional responses, but also frost damage. After the first freezing event, gas exchange was uninterrupted. Consequently, intercellular spaces are free of ice, and ice may be restricted to xylem vessels. The cellular shape of the mesophyll cells was unchanged, cells did not freeze-dehydrate but were supercooled. When thawed after the first freezing event, leaves were initially photoinhibited but regained photosynthesis. During the second freezing event, cells froze intracellularly, and some palisade parenchyma cells remained intact for a prolonged time. Intracellular ice caused complete destruction of cells, and chloroplasts became invisible at the light microscopic level. When thawed after the second freezing, leaves were unable to regain photosynthesis. Consequently, freezing avoidance is the only viable strategy for potatoes to survive frost.","PeriodicalId":89376,"journal":{"name":"GM crops","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84313715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
GM cropsPub Date : 2022-09-30DOI: 10.3390/crops2040025
M. Nunes da Silva, M. Santos, M. Vasconcelos, S. Carvalho
{"title":"Mitigation of Emergent Bacterial Pathogens Using Pseudomonas syringae pv. actinidiae as a Case Study—From Orchard to Gene and Everything in Between","authors":"M. Nunes da Silva, M. Santos, M. Vasconcelos, S. Carvalho","doi":"10.3390/crops2040025","DOIUrl":"https://doi.org/10.3390/crops2040025","url":null,"abstract":"Globalization propelled human migration and commercial exchanges at the global level, but woefully led to the introduction of non-indigenous organisms into several agroecological systems. These include pathogenic bacteria with devastating consequences for numerous crops of agronomical importance for food production worldwide. In the last decade, research efforts have focused on these noxious organisms, aiming to understand their evolutionary processes, degree of pathogenicity, and mitigation strategies, which have allowed stakeholders and policymakers to develop evidence-based regulatory norms to improve management practices and minimize production losses. One of these cases is the bacterium Pseudomonas syringae pv. actinidiae (Psa), the causal agent of the kiwifruit bacterial canker, which has been causing drastic production losses and added costs related to orchard management in the kiwifruit industry. Although Psa is presently considered a pandemic pathogen and far from being eradicated, the implementation of strict regulatory norms and the efforts employed by the scientific community allowed the mitigation, to some extent, of its negative impacts through an integrated pest management approach. This included implementing directive guidelines, modifying cultural practices, and searching for sources of plant resistance. However, bacterial pathogens often have high spatial and temporal variability, with new strains constantly arising through mutation, recombination, and gene flow, posing constant pressure to agroecosystems. This review aims to critically appraise the efforts developed to mitigate bacterial pathogens of agronomical impact, from orchard management to genome analysis, using Psa as a case study, which could allow a prompter response against emerging pathogens in agroecosystems worldwide.","PeriodicalId":89376,"journal":{"name":"GM crops","volume":"94 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87255821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
GM cropsPub Date : 2022-09-22DOI: 10.3390/crops2040024
Chelsea Maier, Zhongwen Chen, C. Cazzonelli, D. Tissue, O. Ghannoum
{"title":"Precise Phenotyping for Improved Crop Quality and Management in Protected Cropping: A Review","authors":"Chelsea Maier, Zhongwen Chen, C. Cazzonelli, D. Tissue, O. Ghannoum","doi":"10.3390/crops2040024","DOIUrl":"https://doi.org/10.3390/crops2040024","url":null,"abstract":"Protected cropping produces more food per land area than field-grown crops. Protected cropping includes low-tech polytunnels utilizing protective coverings, medium-tech facilities with some environmental control, and high-tech facilities such as fully automated glasshouses and indoor vertical farms. High crop productivity and quality are maintained by using environmental control systems and advanced precision phenotyping sensor technologies that were first developed for broadacre agricultural and can now be utilized for protected-cropping applications. This paper reviews the state of the global protected-cropping industry and current precision phenotyping methodology and technology that is used or can be used to advance crop productivity and quality in a protected growth environment. This review assesses various sensor technologies that can monitor and maintain microclimate parameters, as well as be used to assess plant productivity and produce quality. The adoption of precision phenotyping technologies is required for sustaining future food security and enhancing nutritional quality.","PeriodicalId":89376,"journal":{"name":"GM crops","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88754602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
GM cropsPub Date : 2022-09-14DOI: 10.3390/crops2030023
T. Watabe, Y. Nakano, D. Ahn
{"title":"The Effect of Electrical Conductivity on Fruit Growth Pattern in Hydroponically Grown Tomatoes","authors":"T. Watabe, Y. Nakano, D. Ahn","doi":"10.3390/crops2030023","DOIUrl":"https://doi.org/10.3390/crops2030023","url":null,"abstract":"Osmotic stress enhances fruit quality, including the dry matter content, in tomatoes (Solanum lycopersicum L.). This study aimed at providing further insight into the precision control of fruit yield and quality on the long-term moderate osmotic stress conditions in tomato fruit production. We compared the growth pattern between fruits of two cultivars, typical Japanese and Dutch cultivars, under two different nutrient concentrations (2.3 and 5.0 dS·m−1) to understand the effect of electrical conductivity (EC) on dry mass and water content of fruits. The experiment was performed with a rockwool bag culture system in a controlled greenhouse. Increasing EC resulted in an approximately 20% decrease in fruit yield and a 0.5–1% increase in fruit dry matter content in both cultivars. This yield reduction was not caused by the fruit number, but by an approximately 25% decrease in individual fresh fruit weight. Non-linear models were used to describe the changes in dry matter content, water content, and dry weight of tomato fruit as a function of cumulative temperature. The decay rate of dry matter content in the fruit decreased with high EC treatments in the Japanese cultivar. The points at which the rates of changes in water and dry weight increased the in fruit were around 585 and 480 °C-days after anthesis, respectively, under the low EC condition. Rates of water increase in the fruit were changed by high EC treatment, while the shifts were opposite with respect to the cultivars. Dry weight increase in the fruit was not affected by EC treatment. Collectively, our findings clarify the effect of EC on the fruit growth characteristics of Japanese and Dutch tomato cultivars, and provide new insights into the yield of high-Brix tomato cultivation.","PeriodicalId":89376,"journal":{"name":"GM crops","volume":"102 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85823997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
GM cropsPub Date : 2022-08-29DOI: 10.3390/crops2030022
C. E. da Silva Oliveira, T. Zoz, A. Jalal, Eduardo Pradi Vendruscolo, T. Nogueira, A. Jani, M. C. T. Teixeira Filho
{"title":"Tolerance and Adaptability of Tomato Genotypes to Saline Irrigation","authors":"C. E. da Silva Oliveira, T. Zoz, A. Jalal, Eduardo Pradi Vendruscolo, T. Nogueira, A. Jani, M. C. T. Teixeira Filho","doi":"10.3390/crops2030022","DOIUrl":"https://doi.org/10.3390/crops2030022","url":null,"abstract":"High salt concentration in irrigation water is often a limiting factor to tomato production in Brazil. However, there is limited information available regarding the tolerance of tomato genotypes to saline irrigation. An experiment was conducted in a protected environment using a randomized block design with four replications. Treatments consisted of 12 tomato genotypes cultivated in an environment with varying levels of salt stress. Moderate and severe salt stress affected plant height, transversal and longitudinal diameter of fruit, fresh mass, yield, and number of tomato fruit per plant. Cluster analysis, stability, and adaptability provided the best estimates to identify the most adaptable genotype to saline stress, with the genotypes Maestrina, Onix, Pizzadoro, and Shanty being the best adapted to moderate and severe saline stress conditions. The genotypes Maestrina, Onix, Pizzadoro, and Shanty were identified as most adaptable to and stable under salt stress. Sodium absorption increased as irrigation salinity increased. In addition, P, K, and Ca concentration decreased under salt stress, which caused damage to all yield components and plant nutrition. The genotype Onix was more tolerant to the effects of moderate saline irrigation, while the genotypes Sheena, Sperare, Santa Clara, IPA 6, and Dominador had lower losses under severe salt stress conditions.","PeriodicalId":89376,"journal":{"name":"GM crops","volume":"78 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77192531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
GM cropsPub Date : 2022-08-29DOI: 10.3390/crops2030021
Madalina Neacsu, Shirley De Lima Sampaio, H. Hayes, G. Duncan, N. Vaughan, W. Russell, Vassilios Raikos
{"title":"Nutritional Content, Phytochemical Profiling, and Physical Properties of Buckwheat (Fagopyrum esculentum) Seeds for Promotion of Dietary and Food Ingredient Biodiversity","authors":"Madalina Neacsu, Shirley De Lima Sampaio, H. Hayes, G. Duncan, N. Vaughan, W. Russell, Vassilios Raikos","doi":"10.3390/crops2030021","DOIUrl":"https://doi.org/10.3390/crops2030021","url":null,"abstract":"The adoption of food crops as a source of dietary macro- and micro-nutrients is a sustainable way to promote diet biodiversity and health while being respectful to the environment. The aim of this work was to comprehensively characterize the nutrient and phytochemical content of buckwheat seeds (Fagopyrum esculentum) and assess their physical properties for the evaluation of their suitability as food ingredients. The buckwheat samples were found to be complete sources of amino acids (UPLC-TUV analysis), with a protein content between 11.71 ± 0.40% and 14.13 ± 0.50% (Vario Max CN analysis), and a source of insoluble fiber with 11.05 ± 0.25 %, in the UK hulled samples (GC analysis). The buckwheat samples were rich in phosphorus, manganese, molybdenum, zinc, magnesium, and selenium (ICP-MS analysis). A total of 196 plant metabolites were detected using HPLC and LCMS analysis, with anthocyanidins (pelargonidin and cyanidin) being the most abundant phenolic molecules that were measured in all the buckwheat samples. Removing the hull was beneficial for increasing the powder bulk density, whereas the hulled buckwheat samples were more easily rehydrated. The implementation of buckwheat as a staple food crop has enormous potential for the food industry, human nutrition, and diet diversification and could contribute towards meeting the daily recommendation for dietary fiber, essential amino acids, and minerals in Western-style diet countries such as the UK.","PeriodicalId":89376,"journal":{"name":"GM crops","volume":"76 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88209971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
GM cropsPub Date : 2022-08-05DOI: 10.3390/crops2030020
C. Bartel, K. Moore, Shui‐zhang Fei, A. Lenssen, R. Hintz, Samantha M. Kling
{"title":"Evaluating Strip and No-Till Maintenance of Perennial Groundcovers for Annual Grain Production","authors":"C. Bartel, K. Moore, Shui‐zhang Fei, A. Lenssen, R. Hintz, Samantha M. Kling","doi":"10.3390/crops2030020","DOIUrl":"https://doi.org/10.3390/crops2030020","url":null,"abstract":"Perennial groundcover (PGC) merges scalable soil and water conservation with high-yielding row crops, enhancing ecosystem services of annual grain crop production. However, ineffective groundcover suppression increases competition between the groundcover and row crop, reducing row crop grain yield. The objective of this study was to assess the effectiveness of three Kentucky bluegrass (KBG) groundcover suppression methods each at narrow and wide widths on maize (Zea mays L.) growth and development in evenly spaced PGC, compared to alternating PGC swards and a no-PGC conventional tillage control. Suppression methods for evenly spaced PGC included two different strip tillage implements, completing either shallow or deep soil fracture, for mechanical suppression and a banded sprayer for no-tillage chemical suppression. We measured weekly for maize plant height, phenological stage of development, reflected red:far-red (R:FR) ratio, early vegetative and final plant density, grain and stover yield, yield components of kernel rows ear−1, kernels row−1, kernels ear−1, ear length, kernel weight, grass frequency, and weed community. In 2020, maize grain yield in the alternating PGC swards (11.38 Mg ha−1) was similar to the control (12.78 Mg ha−1) and greater than in the evenly spaced groundcover (9.62 Mg ha−1). Maize grain yield was similar for systems in 2021 (7.41 Mg ha−1), due to drought and high coefficient of variation. Weed community was similar for systems in both years. A maize competition response was observed for both suppression widths across methods. Groundcover dormancy may be needed in conjunction with effective chemical and/or mechanical groundcover suppression to support maize production in PGC.","PeriodicalId":89376,"journal":{"name":"GM crops","volume":"66 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86832457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}