Biomedical Journal最新文献_第3页

Pseudogene: Relevant or Irrelevant? 伪基因：相关还是不相关？

IF 4.1 3区医学

Biomedical Journal Pub Date : 2024-09-19 DOI: 10.1016/j.bj.2024.100790

Yang-Hsiang Lin, Chau-Ting Yeh, Cheng-Yi Chen, Kwang-Huei Lin

引用次数: 0

Biological functions of extracellular vesicles from mammalian cells 哺乳动物细胞外囊泡的生物功能

IF 4.1 3区医学

Biomedical Journal Pub Date : 2024-09-12 DOI: 10.1016/j.bj.2024.100788

Jean M. Kanellopoulos, Frédéric Rieux-Laucat, David M. Ojcius

{"title":"Biological functions of extracellular vesicles from mammalian cells","authors":"Jean M. Kanellopoulos, Frédéric Rieux-Laucat, David M. Ojcius","doi":"10.1016/j.bj.2024.100788","DOIUrl":"10.1016/j.bj.2024.100788","url":null,"abstract":"<div><p>Extracellular vesicles (EVs) are enclosed by a phospholipid bilayer and can be secreted by most types of cells. EVs deliver cargo from the secreting cell into the cytoplasm of recipient cells, influencing the function of the recipient cells. EVs are attracting increasing attention from a broad range of clinicians and scientists due to their ability to promote or inhibit various physiological pathways or pathological conditions. This special issue of <em>Biomedical Journal</em> contains articles describing the biogenesis and biodistribution of EVs and their role in the intercellular transfer of various molecules or viruses to target cells, in rejecting allogeneic transplants and maintaining immune tolerance of the allogeneic fetus, and in modulating innate and adaptive immunity. Characterization of the role of EVs in various pathological conditions and our ability to engineer modified EVs may lead to discovery of novel biomarkers and development of therapeutic strategies for treatment of disease.</p></div>","PeriodicalId":8934,"journal":{"name":"Biomedical Journal","volume":"47 5","pages":"Article 100788"},"PeriodicalIF":4.1,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S231941702400091X/pdfft?md5=a66d3b73e737d5696a9075543f2fd341&pid=1-s2.0-S231941702400091X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142242049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of recombinant SARS-CoV-2 spike protein variants on red blood cells parameters and red blood cell distribution width 重组 SARS-CoV-2 穗状病毒蛋白变体对红细胞参数和红细胞分布宽度的影响

IF 4.1 3区医学

Biomedical Journal Pub Date : 2024-09-07 DOI: 10.1016/j.bj.2024.100787

Francesco Dima, Gian Luca Salvagno, Giuseppe Lippi

引用次数: 0

Advancements in Chondrocyte 3-Dimensional Embedded Culture: Implications for Tissue Engineering and Regenerative Medicine. 软骨细胞三维嵌入培养的进展：组织工程和再生医学的意义》。

IF 4.1 3区医学

Biomedical Journal Pub Date : 2024-09-03 DOI: 10.1016/j.bj.2024.100786

Yu-Ying Chu, Atsuhiko Hikita, Yukiyo Asawa, Kazuto Hoshi

{"title":"Advancements in Chondrocyte 3-Dimensional Embedded Culture: Implications for Tissue Engineering and Regenerative Medicine.","authors":"Yu-Ying Chu, Atsuhiko Hikita, Yukiyo Asawa, Kazuto Hoshi","doi":"10.1016/j.bj.2024.100786","DOIUrl":"https://doi.org/10.1016/j.bj.2024.100786","url":null,"abstract":"<p><p>Cartilage repair necessitates regenerative medicine because of the unreliable healing mechanism of cartilage. To yield a sufficient number of cells for transplantation, chondrocytes must be expanded in culture. However, in 2D culture, chondrocytes tend to lose their distinctive phenotypes and functionalities after serial passage, thereby limiting their efficacy for tissue engineering purposes. The mechanism of dedifferentiation in 2D culture can be attributed to various factors, including abnormal nuclear strength, stress-induced mitochondrial impairment, chromatin remodeling, ERK-1/2 and the p38/mitogen-activated protein kinase (MAPK) signaling pathway. These mechanisms collectively contribute to the loss of chondrocyte phenotype and reduced production of cartilage-specific extracellular matrix (ECM) components. Chondrocyte 3D culture methods have emerged as promising solutions to prevent dedifferentiation. Techniques, such as scaffold-based culture and scaffold-free approaches, provide chondrocytes with a more physiologically relevant environment, promoting their differentiation and matrix synthesis. These methods have been used in cartilage tissue engineering to create engineered cartilage constructs for transplantation and joint repair. However, chondrocyte 3D culture still has limitations, such as low viability and proliferation rate, and also difficulties in passage under 3D condition. These indicate challenges of obtaining a sufficient number of chondrocytes for large-scale tissue production. To address these issues, ongoing studies of many research groups have been focusing on refining culture conditions, optimizing scaffold materials, and exploring novel cell sources such as stem cells to enhance the quality and quantity of engineered cartilage tissues. Although obstacles remain, continuous endeavors to enhance culture techniques and overcome limitations offer a promising outlook for the advancement of more efficient strategies for cartilage regeneration.</p>","PeriodicalId":8934,"journal":{"name":"Biomedical Journal","volume":" ","pages":"100786"},"PeriodicalIF":4.1,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142139218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular vesicles in fetal-maternal immune tolerance "胎儿和母体免疫耐受中的细胞外囊泡

IF 4.1 3区医学

Biomedical Journal Pub Date : 2024-08-29 DOI: 10.1016/j.bj.2024.100785

William J. Burlingham

引用次数: 0

miRNA-mediated regulation of clock gene expression in men and women with colorectal cancer and possible consequences for disease management. miRNA 介导的男性和女性结直肠癌患者时钟基因表达调控及对疾病管理的可能影响。

IF 4.1 3区医学

Biomedical Journal Pub Date : 2024-08-10 DOI: 10.1016/j.bj.2024.100784

Iveta Herichová

{"title":"miRNA-mediated regulation of clock gene expression in men and women with colorectal cancer and possible consequences for disease management.","authors":"Iveta Herichová","doi":"10.1016/j.bj.2024.100784","DOIUrl":"https://doi.org/10.1016/j.bj.2024.100784","url":null,"abstract":"<p><strong>Background: </strong>The incidence and mortality of colorectal cancer (CRC) are persistently higher in men than in women. CRC malignancy is strongly influenced by small non-coding RNAs (miRNAs). Moreover, deregulation of the circadian molecular oscillator has been associated with CRC facilitation. To analyse possible cumulative effects of the above-mentioned factors on CRC progression, we focused on functions of sex-biased miRNAs associated with the clock genes per2 and/or cry2, which are involved in the cell cycle control and DNA damage response.</p><p><strong>Major findings: </strong>We identified miR-24, miR-92a, miR-181a, and miR-21 associated with per2 that are up-regulated in transformed colon tissue of men. miR-93, miR-17, miR-20a, and miR-24 with higher expression in males compared to females were linked to cry2. All these miRNAs possess oncogenic potential and exert their effects mainly via inhibition of the tumour suppressors phosphatase and tensin homolog (PTEN) and/or p53. Down-regulation of PTEN and p53 in men was further strengthened by inhibition of tumour suppressor per2. Oncogenic up-regulated miRNAs associated with per2 or cry2 in the transformed colon tissue of women were not detected.</p><p><strong>Conclusion: </strong>We conclude that the cancer-promoting, sex-biased miRNAs miR-24, miR-92a, miR-181a, miR-93, miR-17, miR-20a, and miR-21 associated with clock genes per2 and/or cry2 can contribute to the sex-dependent development of CRC via inhibition of the PTEN and p53 pathways.</p>","PeriodicalId":8934,"journal":{"name":"Biomedical Journal","volume":" ","pages":"100784"},"PeriodicalIF":4.1,"publicationDate":"2024-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141970576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Prognosis of Patients with Myocardial Infarction after Light Therapy: A Preliminary Study. 光疗后心肌梗死患者的预后：初步研究。

IF 4.1 3区医学

Biomedical Journal Pub Date : 2024-08-07 DOI: 10.1016/j.bj.2024.100783

Wei-Chih Chin, Yu-Shu Huang, Lung-Sheng Wu, Kuang-Tso Lee, Chien-Te Ho, Chen Lin, Wei-Sheng Yang, I-Hang Chung, Pao-Hsien Chu

{"title":"The Prognosis of Patients with Myocardial Infarction after Light Therapy: A Preliminary Study.","authors":"Wei-Chih Chin, Yu-Shu Huang, Lung-Sheng Wu, Kuang-Tso Lee, Chien-Te Ho, Chen Lin, Wei-Sheng Yang, I-Hang Chung, Pao-Hsien Chu","doi":"10.1016/j.bj.2024.100783","DOIUrl":"https://doi.org/10.1016/j.bj.2024.100783","url":null,"abstract":"<p><strong>Background: </strong>Patients with myocardial infarction (MI) can have disturbed sleep, but little is known about the efficacy of light therapy on sleep and prognosis of patients with MI. We conducted a randomized controlled study to investigate its efficacy.</p><p><strong>Material and methods: </strong>This preliminary study included 34 patients with MI. They were randomized into the blue light and the white light groups during their stay in intensive care unit. 17 age and gender matched healthy controls were also enrolled. Actigraphy was used to evaluate objective sleep since enrollment. Delirium scales were used to screen delirium. Lab work-up including vitamin D level was performed at the baseline and discharge. We used Mann-Whitney U test or Wilcoxon signed-rank test to compare the difference between the MI group and the healthy control group, and the group difference after receiving light therapy.</p><p><strong>Results: </strong>Patients with MI had significantly lower vitamin D level than healthy controls (p<0.001). They also had significantly poorer sleep, as indicated by actigraphy parameters including sleep onset latency (p=0.01), sleep efficiency (p=0.002), wake after sleep onset (p<0.001) and awake times (p=0.002). No significant group difference was found by actigraphy after light therapy except a non-significant higher relative amplitude of the blue light group (p=0.061). Besides, vitamin D level of the blue light group increased significantly (p1=0.047, p2=0.045).</p><p><strong>Conclusions: </strong>Patients with MI had poorer sleep, highlighting the needs to develop interventions. Significantly increased vitamin D level and a non-significant better rest-active rhythm after light therapy suggest its potential with sleep and prognosis which warrants further investigation.</p>","PeriodicalId":8934,"journal":{"name":"Biomedical Journal","volume":" ","pages":"100783"},"PeriodicalIF":4.1,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141911577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ancient wisdom and modern innovations: Methods of administering healing 古代智慧与现代创新：治疗方法。

IF 4.1 3区医学

Biomedical Journal Pub Date : 2024-08-01 DOI: 10.1016/j.bj.2024.100773

Aila Akosua Kattner

引用次数: 0

Inhibition of infantile hemangioma growth and promotion of apoptosis via VEGF/PI3K/Akt axis by 755-nm long-pulse alexandrite laser 755nm长脉冲亚历山大宝石激光通过VEGF/PI3K/Akt轴抑制婴儿血管瘤生长和促进细胞凋亡。

IF 4.1 3区医学

Biomedical Journal Pub Date : 2024-08-01 DOI: 10.1016/j.bj.2023.100675

{"title":"Inhibition of infantile hemangioma growth and promotion of apoptosis via VEGF/PI3K/Akt axis by 755-nm long-pulse alexandrite laser","authors":"","doi":"10.1016/j.bj.2023.100675","DOIUrl":"10.1016/j.bj.2023.100675","url":null,"abstract":"<div><h3>Background</h3><p>Infantile hemangioma (IH) is a common vascular tumor in female infants, which can lead to aesthetic issues and facial scarring. This study aimed to investigate the inhibitory effects and underlying mechanisms of 755 nm long-pulsed alexandrite laser on IH.</p></div><div><h3>Methods</h3><p>Hemangioma endothelial cells (HemECs) were exposed to 755 nm long-pulsed alexandrite laser to evaluate its impact on cell proliferation and apoptosis. A patient-derived xenograft model was established to assess the inhibitory effects of laser treatment on IH <em>in vivo</em>.</p></div><div><h3>Results</h3><p><em>In vitro</em>, 755 nm long-pulsed alexandrite laser effectively suppressed the proliferation of HemECs and induced cell apoptosis. Laser treatment significantly inhibited the volume and weight of tumors, accompanied by significant downregulation of vascular endothelial growth factor A (VEGFA), vascular endothelial growth factor receptor 2 (VEGFR2), phosphatidylinositol 3-kinase (PI3K), and protein kinase B (Akt) expression levels in both hemangioma cells and tumors. Additionally, laser treatment resulted in the conversion of VEGFA<sub>165a</sub> to VEGFA<sub>165b</sub>. TUNEL staining demonstrated increased apoptosis in tumor cells after laser treatment, along with upregulation of cleaved caspase-3 and Bax, and downregulation of Bcl-2.</p></div><div><h3>Conclusion</h3><p>In addition to the principle of selective photothermal decomposition, modulation of the VEGF/PI3K/Akt axis may serve as a potential mechanism for IH treatment using a long pulse-width 755 nm laser. This sheds valuable light on the molecular mechanisms underlying IH pathogenesis and potential therapeutic targets while providing a theoretical basis for the safe and efficient management of proliferative IH using laser therapy.</p></div>","PeriodicalId":8934,"journal":{"name":"Biomedical Journal","volume":"47 4","pages":"Article 100675"},"PeriodicalIF":4.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2319417023001129/pdfft?md5=dc4d9893747f22df17b764f630012336&pid=1-s2.0-S2319417023001129-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72013337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Msi2 enhances muscle dysfunction in a myotonic dystrophy type 1 mouse model Msi2增强1型肌营养不良小鼠模型中的肌肉功能障碍。

IF 4.1 3区医学

Biomedical Journal Pub Date : 2024-08-01 DOI: 10.1016/j.bj.2023.100667

{"title":"Msi2 enhances muscle dysfunction in a myotonic dystrophy type 1 mouse model","authors":"","doi":"10.1016/j.bj.2023.100667","DOIUrl":"10.1016/j.bj.2023.100667","url":null,"abstract":"<div><h3>Background</h3><p>Myotonic dystrophy type 1 (DM1) is a rare neuromuscular disease caused by a CTG repeat expansion in the 3′ untranslated region of the <em>DM1 protein kinase</em> gene. Characteristic degenerative muscle symptoms include myotonia, atrophy, and weakness. We previously proposed an Musashi homolog 2 (MSI2)>miR-7>autophagy axis whereby MSI2 overexpression repressed miR-7 biogenesis that subsequently de-repressed muscle catabolism through excessive autophagy. Because the DM1 HSA<sup>LR</sup> mouse model expressing expanded CUG repeats shows weak muscle-wasting phenotypes, we hypothesized that MSI2 overexpression was sufficient to promote muscle dysfunction <em>in vivo.</em></p></div><div><h3>Methods</h3><p>By means of recombinant AAV murine MSI2 was overexpressed in neonates HSA<sup>LR</sup> mice skeletal muscle to induce DM1-like phenotypes.</p></div><div><h3>Results</h3><p>Sustained overexpression of the murine MSI2 protein in HSA<sup>LR</sup> neonates induced autophagic flux and expression of critical autophagy proteins, increased central nuclei and reduced myofibers area, and weakened muscle strength. Importantly, these changes were independent of MBNL1, MBNL2, and Celf1 protein levels, which remained unchanged upon Msi2 overexpression.</p></div><div><h3>Conclusions</h3><p>Globally, molecular, histological, and functional data from these experiments in the HSA<sup>LR</sup> mouse model confirms the pathological role of MSI2 expression levels as an atrophy-associated component that impacts the characteristic muscle dysfunction symptoms in DM1 patients.</p></div>","PeriodicalId":8934,"journal":{"name":"Biomedical Journal","volume":"47 4","pages":"Article 100667"},"PeriodicalIF":4.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S231941702300104X/pdfft?md5=1be53c3ab977b1e60a3e8df7bda4edd9&pid=1-s2.0-S231941702300104X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41101531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0