Bioscience Reports最新文献

{"title":"The cytoskeleton of pathogenic protists.","authors":"Wanderley de Souza, Ana Paula Gadelha, Marlene Benchimol","doi":"10.1042/BSR20254047","DOIUrl":"10.1042/BSR20254047","url":null,"abstract":"<p><p>The cytoskeleton is a key component of eukaryotic cells, including protists. It is composed of microtubules, microfilaments, and intermediate filaments. Here, we review the available information on the cytoskeleton of several relevant pathogenic protists, including Trypanosomatidae, Apicomplexa, Trichomonadidae, Giardia intestinalis, and Entamoeba histolytica. In protists, the first two components, made of tubulins and actin, predominate. Usually, they associate with each other and with other components to form complex structures. Emphasis is given to the following structures: flagellum, flagellar-cell body adhesion zone, paraflagellar rod, sub-pellicular microtubules, cytostome, conoid, adhesive disc, funis, median body, costa, axostyle, parabasal filaments, and clockwise filaments. On the other hand, filamentous structures made of not yet completely characterized proteins form structures such as the costa. Each structure is analyzed using morphological information obtained through modern microscopy techniques and biochemical data.</p>","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":"46 4","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13071381/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147688081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering extracellular vesicles for targeted therapeutic delivery in the heart.","authors":"Iqra Anwar, Xinghua Wang, Saverio Parlongo, Sonalí Harris, Richard E Pratt, Victor J Dzau, Conrad P Hodgkinson","doi":"10.1042/BSR20254075","DOIUrl":"10.1042/BSR20254075","url":null,"abstract":"<p><p>Heart failure is a leading cause of morbidity and mortality, highlighting the need for improved therapeutic strategies. Critical to the success of therapies is efficient and targeted delivery systems. Extracellular vesicle-based delivery systems have emerged as promising candidates due to their biocompatibility and low immunogenicity. While extracellular vesicles from a wide variety of cells have been used, they have demonstrated divergent effects on the heart. The present review first summarizes the current sources of extracellular vesicles employed in heart failure therapy and their contrasting outcomes. The review then examines the view that these contrasting outcomes arise from limited cell specificity, inefficient delivery, and suboptimal cargo loading. Finally, the review discusses how these problems are being dealt with by recent advances, including genetic modification, chemical functionalization, and enhanced loading strategies. Together, these approaches highlight the potential of extracellular vesicle-based systems as precision therapeutics in cardiovascular medicine.</p>","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":"46 4","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13136563/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147761264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction: Ivermectin induces autophagy-mediated cell death through the AKT/mTOR signaling pathway in glioma cells.","authors":"Aili Gao, Jingjing Liu, Hongsheng Liang, Chen Chen, Xiaoxing Wang, Faling Qu, Haiyang Wang, Kongbin Yang, Qing Wang, Ning Zhao, Jing Meng","doi":"10.1042/BSR20192489_RET","DOIUrl":"10.1042/BSR20192489_RET","url":null,"abstract":"","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":"46 4","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13089784/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147509411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Poly r(C) binding protein (PCBP) 1 expression is regulated by the E3 ligase UBE4A in thyroid carcinoma.","authors":"Ming-Peng Zhang, Zaozhi Song, Wei-San Zhang, Jin Tan, Ming-Hui Zhao, Lin-Juan Lian, Jie Cai","doi":"10.1042/BSR20170114_RET","DOIUrl":"10.1042/BSR20170114_RET","url":null,"abstract":"","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":"46 4","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13089783/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147643945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Observation of aldicarb hydrolysis by a cocaine hydrolase.","authors":"Johnathan E LeSaint, Daniel J Peter, Huimei Wei, Shawn Park, Chang-Guo Zhan, Fang Zheng","doi":"10.1042/BSR20254091","DOIUrl":"10.1042/BSR20254091","url":null,"abstract":"<p><p>Aldicarb is a carbamate pesticide used for pest control in agriculture. As a fast-acting acetylcholinesterase inhibitor, aldicarb interferes with the nervous system by preventing the breakdown of acetylcholine. Aldicarb could be used as a chemical-warfare agent to cause mass casualty incidents. There is no specific FDA-approved medication for aldicarb detoxification. Our previous study revealed that an Fc-fused butyrylcholinesterase (BChE) mutant, known as CocH3-Fc(M3), can be inhibited rapidly by aldicarb and that the aldicarb-inhibited enzyme CocH3-Fc(M3) can be reactivated spontaneously, suggesting that CocH3-Fc(M3) may hydrolyze aldicarb. However, the suggested CocH3-Fc(M3)-catalyzed hydrolysis of aldicarb was not confirmed experimentally in the previous study. In the present study, by developing an LC-MS/MS method to detect and quantify aldicarb and aldicarb oxime concentrations, we were able to directly observe the CocH3-Fc(M3)-catalyzed aldicarb hydrolysis for the first time, confirming that CocH3-Fc(M3) indeed has the desirable catalytic activity for aldicarb hydrolysis and may be considered as the first aldicarb hydrolase identified so far. Further, we carried out Michaelis-Menten kinetic analysis on the CocH3-Fc(M3)-catalyzed aldicarb hydrolysis and determined the catalytic parameters (kcat = 0.060 min-1, KM = 2.5 μM, and kcat/KM = 2.4 × 104 min-1 M-1) at 37°C. The obtained kinetic parameters at 37°C will be valuable for further in vivo studies and translational research using CocH3-Fc(M3) and for designing more potent enzymes to hydrolyze aldicarb in the future. Additionally, the LC-MS/MS method developed in this study may serve as a valuable tool to accurately detect aldicarb and its reaction products in future food and environmental safety control efforts and aldicarb-related toxicology studies.</p>","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13136561/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146225163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction: miR-384 induces apoptosis and autophagy of non-small cell lung cancer (NSCLC) cells through the negative regulation of Collagen alpha-1(X) chain (COL10A1) gene.","authors":"Min Zheng, Qingkui Guo, Ye Xu, Ning Wang, Wen Zhao","doi":"10.1042/BSR20181523_RET","DOIUrl":"10.1042/BSR20181523_RET","url":null,"abstract":"","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":"46 4","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13089781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147509425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Translational control of Toxoplasma gondii differentiation.","authors":"Fengrong Wang","doi":"10.1042/BSR20253672","DOIUrl":"10.1042/BSR20253672","url":null,"abstract":"<p><p>Toxoplasma gondii is a globally prevalent protozoan parasite capable of establishing lifelong infections in its host. While acute infection is often asymptomatic, reactivation of latent bradyzoites can cause severe disease, particularly in immunocompromised individuals. Current therapies are ineffective against chronic infection, underscoring critical gaps in our understanding of bradyzoite biology and the molecular mechanisms governing stage conversion. Recent studies have identified translational control as a central regulator of T. gondii differentiation. This review highlights the roles of canonical translation initiation factors (eIF2α, eIF1.2, and eIF4E1), RNA-binding proteins (RBPs; BFD2/ROCY1, Alba1, and Alba2), and RNA modifications (with pseudouridylation representing the best-characterized modification currently linked to differentiation), as well as alternative splicing and non-coding RNAs in shaping stage-specific translational programs. This review further discusses underexplored mechanisms, including non-canonical initiation pathways, upstream open reading frames, transcript-level RNA modifications, ribosome heterogeneity and rRNA modifications, elongation and termination control, uncharacterized RBPs, and post-translational modifications of translation factors, that may coordinate proteome remodeling during differentiation. Together, established translational regulators and these emerging pathways highlight translational control as a central driver of parasite persistence and a promising therapeutic target for chronic toxoplasmosis.</p>","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":"46 4","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13071378/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147589834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Renal function dynamics in COVID-19: exploring biomarker interactions with D-dimer and C-reactive proteins.","authors":"Ishita Saha, Anirban Sinha, Anup Kumar Sadhu, Rabindra Nath Das, Satadru Ghosh, Priyajit Banerjee, Oly Banerjee, Shampa Sarkar Biswas, Sandip Mukherjee, Palash Kumar Pal","doi":"10.1042/BSR20254002","DOIUrl":"10.1042/BSR20254002","url":null,"abstract":"<p><p>COVID-19, caused by the SARS-CoV-2 virus, is mainly recognized for its respiratory manifestations. However, growing evidence regarding the widespread expression of ACE2 and TMPRSS2 receptors on diverse extrapulmonary sites, particularly in renal tubular epithelial cells, suggests susceptibility of other organ systems, including the kidneys, to such conditions as acute kidney injury (AKI). In the present retrospective study, we explored the interrelationship between disease severity and renal function abnormalities by analyzing key biochemical parameters: blood urea nitrogen (BUN), serum creatinine (Cr), the BUN/Cr ratio, and estimated glomerular filtration rate (eGFR). Using descriptive statistics and joint generalized linear models, we examined both the mean and variance components of these markers alongside inflammatory indicators such as C-reactive protein (CRP) and D-dimer. Our findings revealed a significant positive correlation between serum urea levels and both CRP and D-dimer concentrations, suggesting that elevated urea may reflect heightened inflammatory activity. Additionally, eGFR showed a positive association with CRP, indicating potential renal involvement in systemic inflammation. Our in silico studies supported such observations, as genes responsible for CRP and D-dimer elevation were found to be common in AKI-associated pathways, particularly IL-6/JAK-STAT, NF-κB, HIF-1, and complement pathways, ultimately causing renal microthrombosis, tubular necrosis, and fibrotic remodeling. Notably, serum Cr revealed no significant association with CRP or D-dimer, possibly due to its lower sensitivity in early renal dysfunction. Although the study is limited by a relatively small sample size and lacks longitudinal data, it underscores the importance of monitoring renal function parameters in COVID-19 patients as potential markers of disease progression.</p>","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13136562/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147637860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction: Associations of MMP-2 and MMP-9 gene polymorphism with ulinastatin efficacy in patients with severe acute pancreatitis.","authors":"Zhen-Fu Li, Guo-Dong Zhen, Lian-Bin Zhao, Shan-Shan Wu, Ming-Yu Chen, Zhen-He Li, Sheng-Zhi Zhou","doi":"10.1042/BSR20160612_RET","DOIUrl":"10.1042/BSR20160612_RET","url":null,"abstract":"","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":"46 4","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13089782/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147509398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taenia solium extracellular vesicles decrease reactive oxygen species production in human neutrophils by inhibiting myeloperoxidase activity.","authors":"César Díaz-Godínez, Diana G Ríos-Valencia, Andrea Toledo, Arturo Calderón-Gallegos, Patricia de la Torre, Karel Estrada, Raúl J Bobes, Julio C Carrero, Agnès Fleury, Juan P Laclette","doi":"10.1042/BSR20250355","DOIUrl":"10.1042/BSR20250355","url":null,"abstract":"<p><p>Taenia solium cysticerci are the etiological agents of neurocysticercosis, a leading cause of acquired epilepsy worldwide and a significant public health problem in endemic regions. The chronicity of the infection reflects a complex host-parasite interaction in which the parasite establishes long-term persistence through immunomodulatory mechanisms. In recent years, extracellular vesicles (EVs) have emerged as important mediators for some of these interactions. EVs are membrane-bound structures released by virtually every organism studied, which carry a diverse set of molecules that mediate intercellular communication. We isolated Taenia solium extracellular vesicles (TsEVs) from racemose cysticerci recovered from a patient, subsequently cultured in vitro, in order to characterize them by transmission electron microscopy, nanoparticle tracking analysis, and mass spectrometry-based proteomics. Effect of TsEVs on the production of reactive oxygen species (ROS) and myeloperoxidase (MPO) activity was evaluated in human neutrophils using fluorometric and colorimetric assays under basal and chemically stimulated conditions. Obtained TsEVs were under 200 nm in diameter. Proteomic analysis of four independent TsEV samples allowed identification of a core set of 336 proteins. Gene Ontology analysis of these vesicles was consistent with exosomes, as a number of exosome-associated marker proteins such as tetraspanins were identified. Proteins linked to immunomodulation and redox metabolism were also identified, suggesting a potential role in interactions with host cells. Finally, the effect of TsEVs on the respiratory burst of human neutrophils was evaluated, revealing a reduction in ROS production, primarily through inhibition of MPO activity, adding up evidence for the role of these vesicles in parasite's survival against the host immune response.</p>","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":"46 4","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13071379/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147571995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}