Asynchronous changes of hydrogen sulfide and its generating enzymes in most tissues with the aging process.

IF 3.8 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioscience Reports Pub Date : 2024-10-30 DOI:10.1042/BSR20240320

Kaichuan He, Bo Tan, Ao Lu, Lu Bai, Chengqing Song, Yuxin Miao, Biyu Liu, Qian Chen, Xu Teng, Jing Dai, Yuming Wu

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引用次数: 0

Abstract

Aging is an inevitable and irreversible biological process that gradually heightens the risks of various diseases and death. As a newly discovered endogenous gasotransmitter, hydrogen sulfide (H2S) has been identified to exert multiple beneficial impacts on the regulation of aging and age-related pathologies. This study was aimed at systematically exploring the relationship between asynchronous aging processes and H2S concentrations in various tissues of aging mice. Samples of plasma and 13 tissues were collected from four cross-sectional age groups (3, 6, 12 and 18 months of age) covering the lifespan of male C57BL/6J mice. The H2S concentration was quantified by a reported liquid chromatography-tandem mass spectrometry (LC-MS/MS) method with monobromobimane derivatization. Additionally, the expressions of cystathionine γ-lyase (CSE), cystathionine β-synthase and 3-mercaptopyruvate sulfurtransferase, in those tissues were analyzed by Western blotting. We discovered that the H2S concentrations decreased asynchronously with the aging process in plasma, heart, liver, kidney, spleen, subcutaneous fat and brown fat and increased in brain and lung. At least one of the three H2S-generating enzymes expressions was compensatorily up-regulated with the aging process in most tissues, among which the up-regulation of CSE was the most prominent.

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硫化氢及其生成酶在大多数组织中的变化与衰老过程不同步。

衰老是一个不可避免、不可逆转的生物过程，会逐渐增加各种疾病和死亡的风险。作为一种新发现的内源性气体递质，硫化氢（H2S）已被确认对衰老和年龄相关病症的调控具有多种有益影响。本研究旨在系统探讨非同步衰老过程与衰老小鼠不同组织中 H2S 浓度之间的关系。研究人员从雄性 C57BL/6J 小鼠的四个横断年龄组（3、6、12 和 18 月龄）采集了血浆和十三个组织样本，涵盖了小鼠的整个生命周期。采用已报道的液相色谱-串联质谱（LC-MS/MS）方法和单溴联苯胺（MBB）衍生化技术对 H2S 浓度进行了定量。此外，我们还用 Western 印迹法分析了这些组织中 CSE、CBS 和 3-MST 的表达情况。我们发现，血浆、心脏、肝脏、肾脏、脾脏、皮下脂肪和棕色脂肪中的 H2S 浓度随衰老过程同步降低，而脑和肺中的 H2S 浓度则同步升高。在大多数组织中，三种 H2S 生成酶中至少有一种酶的表达随衰老过程代偿性上调，其中 CSE 的上调最为显著。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioscience Reports 生物-细胞生物学

CiteScore

8.50

自引率

0.00%

发文量

380

审稿时长

6-12 weeks

期刊介绍： Bioscience Reports provides a home for sound scientific research in all areas of cell biology and molecular life sciences. Since 2012, Bioscience Reports has been fully Open Access and publishes all papers under the liberal CC BY licence, giving the life science community quality research to share and discuss.Content before 2012 is subscription-only, and is accessible via archive purchase. Articles are assessed on soundness, providing a home for valid findings and data. We welcome papers that span disciplines (e.g. chemistry, medicine), including papers describing: -new methodologies -tools and reagents to probe biological questions -mechanistic details -disease mechanisms -metabolic processes and their regulation -structure and function -bioenergetics