Pathology research international最新文献_第7页

Plasmin Activation of Glial Cells through Protease-Activated Receptor 1. 通过蛋白酶激活受体1激活神经胶质细胞的纤溶蛋白。

Pathology research international Pub Date : 2013-01-01 Epub Date: 2013-01-28 DOI: 10.1155/2013/314709

André R Greenidge, Kiana R Hall, Ian R Hambleton, Richelle Thomas, Dougald M Monroe, R Clive Landis

{"title":"Plasmin Activation of Glial Cells through Protease-Activated Receptor 1.","authors":"André R Greenidge, Kiana R Hall, Ian R Hambleton, Richelle Thomas, Dougald M Monroe, R Clive Landis","doi":"10.1155/2013/314709","DOIUrl":"https://doi.org/10.1155/2013/314709","url":null,"abstract":"The objective of this study was to determine whether plasmin could induce morphological changes in human glial cells via PAR1. Human glioblastoma A172 cells were cultured in the presence of plasmin or the PAR1 specific activating hexapeptide, SFLLRN. Cells were monitored by flow cytometry to detect proteolytic activation of PAR1 receptor. Morphological changes were recorded by photomicroscopy and apoptosis was measured by annexinV staining. Plasmin cleaved the PAR1 receptor on glial cells at 5 minutes (P = 0.02). After 30 minutes, cellular processes had begun to retract from the basal substratum and by 4 hours glial cells had become detached. Similar results were obtained by generating plasmin de novo from plasminogen. Morphological transformation was blocked by plasmin inhibitors aprotinin or epsilon-aminocaproic acid (P = 0.03). Cell viability was unimpaired during early morphological changes, but by 24 hours following plasmin treatment 22% of glial cells were apoptotic. PAR1 activating peptide SFLLRN (but not inactive isomer FSLLRN) promoted analogous glial cell detachment (P = 0.03), proving the role for PAR1 in this process. This study has identified a plasmin/PAR1 axis of glial cell activation, linked to changes in glial cell morophology. This adds to our understanding of pathophysiological disease mechanisms of plasmin and the plasminogen system in neuroinjury.","PeriodicalId":89212,"journal":{"name":"Pathology research international","volume":"2013 ","pages":"314709"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/314709","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31256874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Upregulation of serum vascular endothelial growth factor in patients with salivary gland tumor. 唾液腺肿瘤患者血清血管内皮生长因子的升高。

Pathology research international Pub Date : 2013-01-01 Epub Date: 2013-07-24 DOI: 10.1155/2013/740582

Azadeh Andisheh Tadbir, Bijan Khademi, Mahyar Malekzadeh, Maryam Mardani, Bahar Khademi

{"title":"Upregulation of serum vascular endothelial growth factor in patients with salivary gland tumor.","authors":"Azadeh Andisheh Tadbir, Bijan Khademi, Mahyar Malekzadeh, Maryam Mardani, Bahar Khademi","doi":"10.1155/2013/740582","DOIUrl":"https://doi.org/10.1155/2013/740582","url":null,"abstract":"Neoangiogenesis is essential for tumor development, invasion, and dissemination. The most potent of the cytokines associated with angiogenesis is vascular endothelial growth factor (VEGF). The aim of the present study was to determine VEGF serum level in patients with salivary gland tumor. Using an ELISA kit, the circulating levels of VEGF in sera from 58 patients with salivary gland tumor and 30 healthy controls were assessed. Mean VEGF levels in sera of patients with salivary gland tumors (574.9 ± 414.3) were significantly higher than those in controls (263.9 ± 310.0) (P = 0.009). Within the salivary gland tumor group, mean serum VEGF concentration in malignant tumors (n = 27) was 727.3 ± 441.8 pg/mL, and that in benign tumors (n = 31) was 442.2 ± 343.3 pg/mL. Mean serum VEGF concentration was significantly higher in malignant tumors than in benign tumors (P = 0.008) and was higher in benign tumors than in controls (P = 0.03). The data in the present study clearly show that VEGF level was consistently upregulated in benign and malignant tumors in comparison to healthy controls. However, the role of VEGF as a prognostic factor in salivary gland tumor and its application in antiangiogenic therapy require further clinical research. ","PeriodicalId":89212,"journal":{"name":"Pathology research international","volume":"2013 ","pages":"740582"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/740582","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31689547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Utility of tissue transglutaminase immunohistochemistry in pediatric duodenal biopsies: patterns of expression and role in celiac disease-a clinicopathologic review. 组织转谷氨酰胺酶免疫组织化学在小儿十二指肠活检中的应用:表达模式及其在乳糜泻中的作用——临床病理综述

Pathology research international Pub Date : 2013-01-01 Epub Date: 2013-09-08 DOI: 10.1155/2013/602985

Saeeda Almarzooqi, Ronald H Houston, Vinay Prasad

{"title":"Utility of tissue transglutaminase immunohistochemistry in pediatric duodenal biopsies: patterns of expression and role in celiac disease-a clinicopathologic review.","authors":"Saeeda Almarzooqi, Ronald H Houston, Vinay Prasad","doi":"10.1155/2013/602985","DOIUrl":"https://doi.org/10.1155/2013/602985","url":null,"abstract":"Tissue transglutaminase (tTG) is a ubiquitous multifunctional protein. It has roles in various cellular processes. tTG is a major target of autoantibodies in celiac disease, and its expression by immunohistochemistry in pediatric celiac disease has not been fully examined. We studied tTG expression in 78 pediatric duodenal biopsies by utilizing an antibody to transglutaminase 2. Serum tTG was positive in all celiac cases evaluated. Serum antiserum endomysial antibody (EMA) and tTG were negative in all control subjects and in inflammatory bowel disease and eosinophilic gastroenteritis. There was a statistically significant difference between cases of celiac disease and normal controls in terms of tTG immunohistochemical staining in duodenal biopsies surface epithelium (P value = 0.0012). There was no significant statistical difference in terms of staining of the villous surface or crypt between the cases of celiac disease and cases with IBD (P value = 0.5970 and 0.5227, resp.). There was no detected correlation between serum tTG values and immunohistochemical positivity on duodenal biopsy in cases of celiac disease (P value = 1). There was no relationship between Marsh classification and positivity of villous surface for tTG (P value = 0.4955). We conclude that tTG has limited utility in diagnosis of celiac disease in pediatric duodenal biopsies. ","PeriodicalId":89212,"journal":{"name":"Pathology research international","volume":"2013 ","pages":"602985"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/602985","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31837422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Cytological and Pathological Correlation of FNAC in Assessing Breast Lumps and Axillary Lymph Node Swellings in a Public Sector Hospital in India. FNAC在印度一家公立医院评估乳腺肿块和腋窝淋巴结肿大中的细胞学和病理学相关性

Pathology research international Pub Date : 2013-01-01 Epub Date: 2013-12-17 DOI: 10.1155/2013/695024

Vasu Reddy Challa, Basavanna Goud Yale Guru, Poornima Rangappa, Vijayalakshmi Deshmane, Devi M Gayathri

{"title":"Cytological and Pathological Correlation of FNAC in Assessing Breast Lumps and Axillary Lymph Node Swellings in a Public Sector Hospital in India.","authors":"Vasu Reddy Challa, Basavanna Goud Yale Guru, Poornima Rangappa, Vijayalakshmi Deshmane, Devi M Gayathri","doi":"10.1155/2013/695024","DOIUrl":"https://doi.org/10.1155/2013/695024","url":null,"abstract":"Background. Breast lumps have varied pathology, and there are different techniques to prove the diagnosis. The aim of the present study is to analyze the role of fine needle aspiration cytology (FNAC) of the breast lesions at our center. Methods. We had retrospectively analysed 854 patients who underwent FNAC for primary breast lumps and 190 patients who underwent FNAC for an axillary lymph node in the year 2010. Results. Of 854 patients, histological correlation was available in 723 patients. The analysis was done for 812 patients as medical records were not available for 42 patients. FNAC was false negative in seven cases; 2 cases of phyllodes were reported as fibroadenoma, and 5 cases of carcinoma were diagnosed as atypical hyperplasia. The sensitivity, specificity, and false negative value of FNAC in diagnosing breast lumps were 99% (715/723), 100%, and 1%, respectively. Of 190 patients for whom FNAC was performed for axilla, 170 had proven to have axillary lymph node metastases, and the rest had reactive hyperplasia or inflammatory cells. Conclusions. FNAC is rapid, accurate, outpatient based, and less complicated procedure and helps in diagnosis of breast cancer, benign diseases, and axillary involvement in experienced hands with less chance of false results. ","PeriodicalId":89212,"journal":{"name":"Pathology research international","volume":"2013 ","pages":"695024"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/695024","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32054059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Internal Quality Assurance Program for ERBB2 (HER2) Testing Improves the Selection of Breast Cancer Patients for Treatment with Trastuzumab. ERBB2 (HER2)检测的内部质量保证计划改善了曲妥珠单抗治疗乳腺癌患者的选择。

Pathology research international Pub Date : 2012-01-01 Epub Date: 2012-02-14 DOI: 10.1155/2012/261857

Vittoria Martin, Antonella Camponovo, Morena Ghisletta, Massimo Bongiovanni, Luca Mazzucchelli

{"title":"Internal Quality Assurance Program for ERBB2 (HER2) Testing Improves the Selection of Breast Cancer Patients for Treatment with Trastuzumab.","authors":"Vittoria Martin, Antonella Camponovo, Morena Ghisletta, Massimo Bongiovanni, Luca Mazzucchelli","doi":"10.1155/2012/261857","DOIUrl":"https://doi.org/10.1155/2012/261857","url":null,"abstract":"International guidelines for ERBB2 (HER2) testing procedures in breast cancer patients highlight the importance of external quality control. In contrast, internal quality assurance programs have been poorly defined, and their clinical significance has not yet been investigated. We developed a quality assurance scheme by performing HER2 FISH on 724 patients randomly selected out of 1996 patients with breast cancer presenting at our institute. We collected samples monthly for tissue microarray analysis and correlated HER2 gene status with IHC scores. The concordance was excellent (κ = 0.92, P < 0.0001). HER2 amplification characterized 25% of score 2+ but also 13% of score 1+, thus expanding the number of patients eligible for trastuzumab. Based on these findings, the FISH test is now recommended at our institution for score 1+ and 2+ patients. Adherence to internal assurance program improves patient selection and may lead to the definition of in-house tailored diagnostic algorithms different from those proposed in international guidelines.","PeriodicalId":89212,"journal":{"name":"Pathology research international","volume":"2012 ","pages":"261857"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/261857","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30528937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Development of Immunopathogenesis Strategies to Treat Behçet's Disease. 免疫发病机制治疗behaperet病的研究进展。

Pathology research international Pub Date : 2012-01-01 Epub Date: 2012-04-03 DOI: 10.1155/2012/261989

Osman Köse

引用次数: 25

Diagnosis of B-cell non-hodgkin lymphomas with small-/intermediate-sized cells in cytopathology. 小/中细胞b细胞非霍奇金淋巴瘤的病理诊断。

Pathology research international Pub Date : 2012-01-01 Epub Date: 2012-05-27 DOI: 10.1155/2012/164934

Joerg Schwock, William R Geddie

{"title":"Diagnosis of B-cell non-hodgkin lymphomas with small-/intermediate-sized cells in cytopathology.","authors":"Joerg Schwock, William R Geddie","doi":"10.1155/2012/164934","DOIUrl":"https://doi.org/10.1155/2012/164934","url":null,"abstract":"Fine needle sampling is a fast, safe, and potentially cost-effective method of obtaining tissue for cytomorphologic assessment aimed at both initial triage and, in some cases, complete diagnosis of patients that present clinically with lymphadenopathy. The cytologic diagnosis of B-cell non-Hodgkin lymphomas composed of small-/intermediate-sized cells, however, has been seen as an area of great difficulty even for experienced observers due to the morphologic overlap between lymphoma and reactive lymphadenopathies as well as between the lymphoma entities themselves. Although ancillary testing has improved diagnostic accuracy, the results from these tests must be interpreted within the morphological and clinical context to avoid misinterpretation. Importantly, the recognition of specific cytologic features is crucial in guiding the appropriate selection of ancillary tests which will either confirm or refute a tentative diagnosis. For these reasons, we here review the cytologic characteristics particular to five common B-cell non-Hodgkin lymphomas which typically cause the most diagnostic confusion based on cytological assessment alone: marginal zone lymphoma, follicular lymphoma, mantle cell lymphoma, chronic lymphocytic leukemia/small lymphocytic lymphoma, and lymphoplasmacytic lymphoma. We summarize the most pertinent cytomorphologic features for each entity as well as for reactive lymphoid hyperplasia, contrast them with each other to facilitate their recognition, and highlight common diagnostic pitfalls.","PeriodicalId":89212,"journal":{"name":"Pathology research international","volume":"2012 ","pages":"164934"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/164934","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30687417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Expression of CD44v6 and Its Association with Prognosis in Epithelial Ovarian Carcinomas. CD44v6在上皮性卵巢癌中的表达及其与预后的关系

Pathology research international Pub Date : 2012-01-01 Epub Date: 2012-02-23 DOI: 10.1155/2012/908206

Dang-Xia Zhou, Yun-Xia Liu, Ya-Hong Xue

{"title":"Expression of CD44v6 and Its Association with Prognosis in Epithelial Ovarian Carcinomas.","authors":"Dang-Xia Zhou, Yun-Xia Liu, Ya-Hong Xue","doi":"10.1155/2012/908206","DOIUrl":"https://doi.org/10.1155/2012/908206","url":null,"abstract":"The aim of this study was to evaluate CD44v6 protein expression and its prognostic value of CD44v6 in ovarian carcinoma. The expression of CD44v6 was analyzed in 62 patients with ovarian carcinoma by immunohistochemical method. The data obtained were analyzed by univariate and multivariate analyses. The present study clearly demonstrates that tumor tissues from 41 (66.1%) patients showed positive expression with CD44v6. The expression of CD44v6 was significantly correlated with histological type, FIGO stage and histological grade of ovarian carcinomas. Concerning the prognosis, the survival period of patients with CD44v6 positive was shorter than that of patients with CD44v6 negative (36.6% versus 66.7%, 5-year survival, P < 0.05). Univariate analysis showed that CD44v6 expression, histological type, FIGO stage and histological grade were associated with 5-year survival, and CD44v6 expression was associated with histological type, FIGO stage and histological grade and 5-year survival. In multivariate analysis, using the COX-regression model, CD44v6 expression was important prognostic factor. In conclusion, these results suggest that CD44v6 may be related to histological type, FIGO stage and histological grade of ovarian carcinomas, and CD44v6 may be an important molecular marker for poor prognosis in ovarian carcinomas.","PeriodicalId":89212,"journal":{"name":"Pathology research international","volume":"2012 ","pages":"908206"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/908206","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30558004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Role of Multiparameter Analysis of AgNORs in FNA Smears of Thyroid Swellings in Differentiating Benign and Malignant Lesions. 甲状腺肿FNA涂片AgNORs多参数分析在良恶性鉴别中的作用。

Pathology research international Pub Date : 2012-01-01 Epub Date: 2012-06-24 DOI: 10.1155/2012/908106

Mohammad Ismail Hossain, Md Quamrul Hassan, Pradip Bhattacharjee, M Shahab Uddin Ahamad, Zillur Rahman

{"title":"Role of Multiparameter Analysis of AgNORs in FNA Smears of Thyroid Swellings in Differentiating Benign and Malignant Lesions.","authors":"Mohammad Ismail Hossain, Md Quamrul Hassan, Pradip Bhattacharjee, M Shahab Uddin Ahamad, Zillur Rahman","doi":"10.1155/2012/908106","DOIUrl":"https://doi.org/10.1155/2012/908106","url":null,"abstract":"Background. The aim of this study is to assess the role of multiparameter analysis of silver (Ag)-stained nucleolar organizer regions (AgNORs) technique on aspiration smears of thyroid swellings to distinguish between benign and malignant lesions. Materials and Methods. Aspiration smears from 166 cases of thyroid swellings were examined. Diagnosis was confirmed by histology in 61 cases. AgNOR staining was done on FNA smears according to silver-staining protocol proposed by the International Committee for AgNOR quantification. Multiparameter analysis of AgNORs such as mAgNOR, pAgNOR, and AgNOR size grade was done on 50-100 cells under oil immersion lens. Results. AgNOR parameter of benign and malignant thyroid lesions was compared and was found to be statistically significant. Out of 157 satisfactory AgNOR stained cases, 148 (94.3%) were benign lesions and 9 (5.7%) cases were malignant lesions. In AgNOR analysis, sensitivity was found to be 83.33%, specificity 100%, PPV 100%, NPV 98.21%, and accuracy was 98.36%. Conclusions. AgNOR analysis in the FNA smears is a simple, sensitive, and cost-effective method for differentiating benign from malignant thyroid swellings.","PeriodicalId":89212,"journal":{"name":"Pathology research international","volume":"2012 ","pages":"908106"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/908106","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30760907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Potential Infectious Etiology of Behçet's Disease. behaperet病的潜在感染病因学。

Pathology research international Pub Date : 2012-01-01 Epub Date: 2011-12-29 DOI: 10.1155/2012/595380

Massimiliano Galeone, Roberta Colucci, Angelo Massimiliano D'Erme, Silvia Moretti, Torello Lotti

{"title":"Potential Infectious Etiology of Behçet's Disease.","authors":"Massimiliano Galeone, Roberta Colucci, Angelo Massimiliano D'Erme, Silvia Moretti, Torello Lotti","doi":"10.1155/2012/595380","DOIUrl":"https://doi.org/10.1155/2012/595380","url":null,"abstract":"Behçet's disease is a multisystem inflammatory disorder characterized by recurrent oral aphthous ulcers, genital ulcers, uveitis, and skin lesions. The cause of Behçet's disease remains unknown, but epidemiologic findings suggest that an autoimmune process is triggered by an environmental agent in a genetically predisposed individual. An infectious agent could operate through molecular mimicry, and subsequently the disease could be perpetuated by an abnormal immune response to an autoantigen in the absence of ongoing infection. Potentia bacterial are Saccharomyces cerevisiae, mycobacteria, Borrelia burgdorferi, Helicobacter pylori, Escherichia coli, Staphylococcus aureus, and Mycoplasma fermentans, but the most commonly investigated microorganism is Streptococcus sanguinis. The relationship between streptococcal infections and Behçet's disease is suggested by clinical observations that an unhygienic oral condition is frequently noted in the oral cavity of Behçet's disease patients. Several viral agents, including herpes simplex virus-1, hepatitis C virus, parvovirus B19, cytomegalovirus, Epstein-Barr virus and varicella zoster virus, may also have some role.","PeriodicalId":89212,"journal":{"name":"Pathology research international","volume":"2012 ","pages":"595380"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/595380","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30393549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 59