{"title":"Significance of Stem Cells in Forensic Dentistry","authors":"B. Karthika, S. Nisa","doi":"10.18311/jfds/13/1/2021.656","DOIUrl":"https://doi.org/10.18311/jfds/13/1/2021.656","url":null,"abstract":"In forensic point of view and for genetic study, biological samples collected at a crime scene serve as a significant tool, in order to resolve crimes by identifying the person. In some scenarios, individual identification gets masked by chimera persons, where the chimera person cells will have different DNA. The use of biological traces which are recorded by a person’s touch while handling items raises the chance of forensic study system. Therefore, DNA profiling can be obtained from items that were touched, which inturn becomes an useful means for forensic mode of investigation. Chimerism investigations are recognized processes to examine the condition of Hematopoietic Stem Cell Transplantation (HSCT) to analyze peripheral blood and recipient’s bone marrow samples for non-malignant and malignant hematologic diseases. In adults, ectomesenchymal cells identified in oral and maxillofacial tissues are promising for future dental stem cell therapies, because the oral tissues area rich source for stem cells. Dental stem cells have various expressive profiles and exist in specific niches. Apart from these applications, this review article highlights dental stem cells significances in forensic dental investigations.","PeriodicalId":89172,"journal":{"name":"Journal of forensic dental sciences","volume":"211 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80652736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparison and Association of Dermatoglyphics and Dental Anomalies in Three Different Regional Populations of India: An Original Study","authors":"S. Kour, N. Grover, N. Singh","doi":"10.18311/jfds/13/1/2021.703","DOIUrl":"https://doi.org/10.18311/jfds/13/1/2021.703","url":null,"abstract":"Aim: The purpose of our study was to investigate and find the association of dental anomalies with the dermatoglyphic pattern within three regional populations of India. Methods: The present study included 600 subjects. 200 from each population were selected randomly and examined for dental anomalies and their dermatoglyphic patterns were recorded. Statistical Analysis Used: The study was analyzed by a chi-square test. Results: One dental anomaly was consistently noted in all three populations. Out of 600 subjects, 40.8% had positional anomalies. Shoveling, congenitally missing teeth, and winging were seen significantly in North-Eastern (23.0%), Jammu & Kashmir (5%), and Western Uttar Pradesh (6%) population groups in order. The most common dermatoglyphic pattern seen in all three populations was an ulnar loop. Significant relation between shoveling and whorl pattern was seen in the North-Eastern population. The presence of rotation was significantly more among subjects with a whorl pattern. Conclusion: The study concluded that dermatoglyphics can be used as an indicator of few dental anomalies. It can help in identifying the gender, race of an unidentified person, and various diseases which are correlated with dental anomalies that can be detected earlier.","PeriodicalId":89172,"journal":{"name":"Journal of forensic dental sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87943444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nadeem Jeddy, T Radhika, L J Sai Lakshmi, Rachel J Khodabux, R Srilekha, G Sruthi
{"title":"Cheiloscopy and dermatoglyphics as screening tools for type 2 diabetes mellitus.","authors":"Nadeem Jeddy, T Radhika, L J Sai Lakshmi, Rachel J Khodabux, R Srilekha, G Sruthi","doi":"10.4103/jfo.jfds_87_19","DOIUrl":"https://doi.org/10.4103/jfo.jfds_87_19","url":null,"abstract":"<p><strong>Aim: </strong>The aim of this study is to evaluate the efficacy of cheiloscopy (the study of lip prints) and dermatoglyphics (the study of fingerprints) in screening diabetic patients.</p><p><strong>Materials and methods: </strong>The study sample comprised 100 individuals in the age group of 17-60 years, of which fifty were diabetics and fifty controls who reported to the Department of Oral Medicine, Thai Moogambigai Dental College and Hospital. Lip prints were collected and categorized based on the Suzuki and Tsuchihashi system. Fingerprint patterns were obtained and classified according to the Henry's system of classification.</p><p><strong>Results: </strong>Type II and IV lip print patterns were predominant in diabetic patients and Type I lip print patterns in controls. The difference was statistically significant. There was no significant difference in fingerprint patterns between the study groups. Gender-wise analysis for lip print and fingerprint patterns did not yield significant results.</p><p><strong>Conclusion: </strong>Cheiloscopy is a potential screening tool for type 2 diabetes mellitus. Dermatoglyphics cannot be used as a screening tool in type 2 diabetes mellitus.</p>","PeriodicalId":89172,"journal":{"name":"Journal of forensic dental sciences","volume":"11 3","pages":"163-166"},"PeriodicalIF":0.0,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/1b/cf/JFDS-11-163.PMC7398358.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38277940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Reflections on setting up forensic odontology department, its activities, and faculty.","authors":"Ashith B Acharya","doi":"10.4103/0975-1475.285791","DOIUrl":"https://doi.org/10.4103/0975-1475.285791","url":null,"abstract":"","PeriodicalId":89172,"journal":{"name":"Journal of forensic dental sciences","volume":"11 3","pages":"167-168"},"PeriodicalIF":0.0,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/5b/fa/JFDS-11-167.PMC7398360.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38278411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A comparative study of sagittal dental relationship using digital method of bite mark evaluation.","authors":"Pallavi Raina, Narayan Kulkarni, Romil Shah","doi":"10.4103/jfo.jfds_80_19","DOIUrl":"https://doi.org/10.4103/jfo.jfds_80_19","url":null,"abstract":"<p><strong>Introduction: </strong>Intercuspation of teeth depends on position of teeth and jaws. Bite mark is the resultant of the intercuspation of teeth produced by an individual.</p><p><strong>Aims and objective: </strong>Comparative evaluation of bite marks in Angle's Class I, II, and III sagittal occlusion.</p><p><strong>Materials and methods: </strong>Three groups as per Angle's classification-Class I, Class II, and Class III relation were taken. Each group comprised 30 samples each. The dental casts were scanned to create digital images. \"IC Measure\" software was used to determine the angular and linear measurements on scanned images of study model after calibration. Internal angles of odontometric triangle, intercanine width, shape of the arch, size, and shape of the individual teeth was recorded and subjected to the statistical analysis.</p><p><strong>Results: </strong>All incisors had rectangular and canine had triangular shape. Bilateral maxillary lateral incisors and mandibular left central incisor were significantly small in size for Class III. Square arch form was found more commonly in Class III and ovoid arch form in Class I and Class II occlusion. Intercanine width was insignificant among all occlusions. All the angles of the odontometric triangle in the maxillary and mandibular arches were significant for Class III.</p><p><strong>Conclusion: </strong>Class III occlusion individuals were distinct for shape of the arch and angles of maxillary and mandibular odontometric triangle. The quantified values of odontometric triangle can be utilized for the identification of Class III individuals.</p>","PeriodicalId":89172,"journal":{"name":"Journal of forensic dental sciences","volume":"11 3","pages":"125-132"},"PeriodicalIF":0.0,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ad/b9/JFDS-11-125.PMC7398363.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38278526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nawal Khan, Radhika M Bavle, Soumya Makarla, Paremala Konda, S R Amulya, Sreenitha S Hosthor
{"title":"Determination of epigenetic age through DNA methylation of NPTX2 gene using buccal scrapes: A pilot study.","authors":"Nawal Khan, Radhika M Bavle, Soumya Makarla, Paremala Konda, S R Amulya, Sreenitha S Hosthor","doi":"10.4103/jfo.jfds_29_19","DOIUrl":"https://doi.org/10.4103/jfo.jfds_29_19","url":null,"abstract":"<p><strong>Context: </strong>DNA methylation (DNAm) age can be used to evaluate the chronological age of individuals often called \"epigenetic age.\" In this study, buccal scrape samples were used for the determination of epigenetic age.</p><p><strong>Aims: </strong>To examine if epigenetic age could be determined using neuronal pentraxin 2 (NPTX2) gene in buccal cells.</p><p><strong>Setting and design: </strong>This cohort study was designed to validate the use of buccal cells for epigenetic age estimation. Sanger sequencing was used to determine the genetic sequence of the gene of interest postamplification. Nucleotide base sequence for NPTX2 gene was obtained for each case using this protocol.</p><p><strong>Subjects and methods: </strong>The study was conducted on buccal scrapes obtained from 26 subjects of both genders, whose age varied from 1 to 65 years. The samples, collected by wooden spatulas, were placed in cell suspension buffer and stored at 4°C until transported to the laboratory.</p><p><strong>Results: </strong>Methylation levels of 5'-C-phosphate-G-3' located in the gene NPTX2 of 26 subjects were studied and analyzed by bisulfate sequencing. The percentage of methylation in this study falls in the range between 15% and 51%.</p><p><strong>Conclusion: </strong>In this study, a sufficient amount of gDNA was retrieved from the buccal cells, thus confirming that buccal scrape was a feasible technique to obtain ample DNA. This study also showed that DNAm-polymerase chain reaction method was a feasible method for the evaluation of methylation pattern of NPTX2 gene.</p>","PeriodicalId":89172,"journal":{"name":"Journal of forensic dental sciences","volume":"11 3","pages":"147-152"},"PeriodicalIF":0.0,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/32/89/JFDS-11-147.PMC7398359.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38278530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Age determination in children by orthopantomograph and lateral cephalogram: A comparative digital study.","authors":"Anand J Patel, Jigna S Shah","doi":"10.4103/jfo.jfds_61_19","DOIUrl":"10.4103/jfo.jfds_61_19","url":null,"abstract":"<p><strong>Background and aims: </strong>The assessment of age is useful in forensic medicine and forensic odontology and in treatment planning in various branch of dentistry. The aim of study is comparative evaluation and assessment of applicability of Demirjian's method, Willem's method of dental age (DA) estimation, and Maria de Paula Caldas's method of skeletal age estimation for children aged 9-16 years.</p><p><strong>Materials and methods: </strong>A total of 140 individuals (70 females and 70 males) between the age group of 9-16 years were enrolled. These individuals were grouped by a difference of 1 year into 7 groups (each group comprising of 20 individuals: 10 males and 10 females). Dental age estimation was performed from orthopantomograph images of mandibular teeth of left quadrant by both Demirjian's and Willem's methods. Skeletal age estimation was done from Lateral Cephalogram by Caldas Digital Method. The differences between the chronological age and the estimated dental and skeletal ages were statistically tested using paired <i>t</i>-test.</p><p><strong>Results: </strong>Demirjian's DA estimation overestimated males (0.4040 years) and females (0.1316 years). Willem's DA estimation method underestimated males (0.1386 years) and females (0.4210 years) and Caldas skeletal age estimation overestimated males (0.2982 years) and females (0.4259 years).</p><p><strong>Conclusion: </strong>The study concluded Willem's DA estimation method was the most accurate for male and Demirjian's method for female for Gujarati Population. Caldas's new computer-assisted method for skeletal age estimation used in the present study is easy to perform and less time-consuming and objective method and can be applied for Gujarati population.</p>","PeriodicalId":89172,"journal":{"name":"Journal of forensic dental sciences","volume":"11 3","pages":"118-124"},"PeriodicalIF":0.0,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/4e/5b/JFDS-11-118.PMC7398366.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38269840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tanya Khaitan, Anjani Kumar Shukla, Prashant Gupta, Shantala R Naik
{"title":"Is panoramic mandibular index a reliable marker in the evaluation of bone mineral density and sexual dimorphism??","authors":"Tanya Khaitan, Anjani Kumar Shukla, Prashant Gupta, Shantala R Naik","doi":"10.4103/jfo.jfds_92_19","DOIUrl":"https://doi.org/10.4103/jfo.jfds_92_19","url":null,"abstract":"<p><strong>Introduction: </strong>Osteoporosis is a silent, progressive, and chronic disease affecting bones of the adults, especially postmenopausal women. Its effect on mandibular bone quality has also been described by some authors in men inferring that gender and age are factors that may influence bone mineral density (BMD) and prognosis. The panoramic radiograph is used widely for the early detection of osteoporosis. The present study was undertaken to evaluate whether the panoramic mandibular index (PMI) is useful for BMD and sexual dimorphism.</p><p><strong>Materials and methods: </strong>A total of 60 patients (30 males and 30 females) in the age group of 25-40 years were selected for the study. Orthopantomograph was taken and PMI determined with the help of Sidexis next-generation software. All the measurements were performed by four observers and data subjected to the statistical analysis.</p><p><strong>Results: </strong>The mean superior PMI was 0.22-0.27, and the inferior PMI was 0.28-0.34. The mean superior and inferior linear measurements from the mental foramen were higher in males than females and statistically significant. The mean mandibular cortical width was 3.8-4.7 mm but did not show much gender difference.</p><p><strong>Conclusion: </strong>PMI is a reliable indicator for determining BMD but is not much influenced by gender variation.</p>","PeriodicalId":89172,"journal":{"name":"Journal of forensic dental sciences","volume":"11 3","pages":"133-136"},"PeriodicalIF":0.0,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e5/73/JFDS-11-133.PMC7398367.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38278527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cone-beam computed tomography a reliable tool for morphometric analysis of the foramen magnum and a boon for forensic odontologists.","authors":"Subhasish Mustafi, Rupam Sinha, Debarati Roy, Suman Sen, Subhadeep Maity, Pritha Ghosh","doi":"10.4103/jfo.jfds_42_19","DOIUrl":"https://doi.org/10.4103/jfo.jfds_42_19","url":null,"abstract":"<p><strong>Background/aim: </strong>The aim of this pilot study was to perform morphometric analysis of the foramen magnum (FM) using cone-beam computed tomography (CBCT).</p><p><strong>Materials and methods: </strong>This study included CBCT images of 120 individuals (60 males and 60 females). The sagittal and transverse diameters and circumference of the FM were measured. The data were statistically analyzed with Chi-square and <i>t</i>-tests to assess the level of significance for sex and age.</p><p><strong>Results: </strong>The means of its sagittal and transverse diameters and also circumference were higher in males than in females. Statistically significant differences were found between transverse and circumference in case of males and females for all variables (<i>P</i> < 0.05). No statistically significant difference (<i>P</i> > 0.05) was found in sagittal diameter and age groups for all variables.</p><p><strong>Conclusion: </strong>CBCT images can provide valuable information regarding FM and the measurements of its sagittal and transvers diameters and also its circumference may be reliably used for sexual dimorphism in anthropometric analysis and forensic medicine.</p>","PeriodicalId":89172,"journal":{"name":"Journal of forensic dental sciences","volume":"11 3","pages":"153-157"},"PeriodicalIF":0.0,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c1/c3/JFDS-11-153.PMC7398362.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38278532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Larissa Padovan, Viviane Ulbricht, Francisco Carlos Groppo, João Sarmento Pereira Neto, Vanessa Moreira Andrade, Luiz Francesquini Júnior
{"title":"Sexual dimorphism through the study of atlas vertebra in the Brazilian population.","authors":"Larissa Padovan, Viviane Ulbricht, Francisco Carlos Groppo, João Sarmento Pereira Neto, Vanessa Moreira Andrade, Luiz Francesquini Júnior","doi":"10.4103/jfo.jfds_85_19","DOIUrl":"https://doi.org/10.4103/jfo.jfds_85_19","url":null,"abstract":"<p><strong>Background: </strong>Sex determination by linear measurements of the bones is widely used because of the several kinds of death in which the corpses can be damaged.</p><p><strong>Aim: </strong>The aim of this study was to establish a logit for sexual dimorphism through measurements of the atlas vertebra.</p><p><strong>Settings and design: </strong>The principle sample was composed of 191 skeletons belonging to the Forensic Physical Anthropology Laboratory Prof. Eduardo Daruge. However, first, a calibration with other 25 skeletons was carried out.</p><p><strong>Materials and methods: </strong>Using a digital caliper, linear measurements were made of the anteroposterior diameter of the atlas vertebra (variable A), anteroposterior diameter of the rachidian canal (variable B), transverse diameter of the rachidian canal (variable C), and maximum transverse diameter of the atlas vertebra (variable D).</p><p><strong>Statistical analysis used: </strong>The data were analyzed using IBM<sup>®</sup> SPSS<sup>®</sup> 25 Statistics program.</p><p><strong>Results: </strong>The mean measurements of all four variables for men were higher than that for women, being observed that variable D obtained the major discrepancy between the sexes. Considering both sexes, the variable C obtained the best results of standard deviation, while the variable D achieved the worse results. The <i>t</i>-test observed acceptance about hypothesis that exists differences between the gender and all four measures assessed. The logit developed is sex = -24.970 + 0.183 × A + 0.230 × D, in which \"A\" represents anteroposterior diameter of the atlas and \"D\" represents the maximum transverse diameter of the atlas.</p><p><strong>Conclusion: </strong>This model results in 81.2% accuracy, 85.5% sensitivity, and 75.3% specificity.</p>","PeriodicalId":89172,"journal":{"name":"Journal of forensic dental sciences","volume":"11 3","pages":"158-162"},"PeriodicalIF":0.0,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/85/8f/JFDS-11-158.PMC7398361.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38277939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}