Biomatter最新文献_第10页

Synthesis and characterization of biocompatible-nanohydroxyapatite crystals obtained by a modified sol-gel processing. 改性溶胶-凝胶法制备生物相容性纳米羟基磷灰石晶体的合成与表征。

Biomatter Pub Date : 2012-04-01 DOI: 10.4161/biom.20379

Ignacio A Figueroa, Omar Novelo-Peralta, Carlos Flores-Morales, Rodrigo González-Tenorio, M Cristina Piña-Barba

引用次数: 7

In vitro degradation and release characteristics of spin coated thin films of PLGA with a "breath figure" morphology. 具有 "呼吸图 "形态的聚乳酸（PLGA）旋涂薄膜的体外降解和释放特性。

Biomatter Pub Date : 2012-04-01 DOI: 10.4161/biom.20390

Thiruselvam Ponnusamy, Louise B Lawson, Lucy C Freytag, Diane A Blake, Ramesh S Ayyala, Vijay T John

{"title":"In vitro degradation and release characteristics of spin coated thin films of PLGA with a \"breath figure\" morphology.","authors":"Thiruselvam Ponnusamy, Louise B Lawson, Lucy C Freytag, Diane A Blake, Ramesh S Ayyala, Vijay T John","doi":"10.4161/biom.20390","DOIUrl":"10.4161/biom.20390","url":null,"abstract":"Poly (lactic-co-glycolic acid) (PLGA) coatings on implant materials are widely used in controlled drug delivery applications. Typically, such coatings are made with non-porous films. Here, we have synthesized a thin PLGA film coating with a highly ordered microporous structure using a simple and inexpensive water templating \"breath figure\" technique. A single stage process combining spin coating and breath figure process was used to obtain drug incorporated porous thin films. The films were characterized by scanning electron microscope (SEM) to observe the surface and bulk features of porosity and also, degradation pattern of the films. Moreover, the effect of addition of small amount of poly (ethylene glycol) (PEG) into PLGA was characterized. SEM analysis revealed an ordered array of ~2 µm sized pores on the surface with the average film thickness measured to be 20 µm. The incorporation of hydrophilic poly (ethylene glycol) (PEG) enhances pore structure uniformity and facilitates ingress of water into the structure. A five week in vitro degradation study showed a gradual deterioration of the breath figure pores. During the course of degradation, the surface pore structure deteriorates to initially flatten the surface. This is followed by the formation of new pinprick pores that eventually grow into a macroporous film prior to film breakup. Salicylic acid (highly water soluble) and Ibuprofen (sparingly water soluble) were chosen as model drug compounds to characterize release rates, which are higher in films of the breath figure morphology rather than in non-porous films. The results are of significance in the design of biodegradable films used as coatings to modulate delivery.","PeriodicalId":8891,"journal":{"name":"Biomatter","volume":"2 2","pages":"77-86"},"PeriodicalIF":0.0,"publicationDate":"2012-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/22/92/biom-2-77.PMC3549860.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31319241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Realization of a poro-elastic ultrasound replica of pulmonary tissue. 实现肺组织的孔弹性超声波复制品。

Biomatter Pub Date : 2012-01-01 DOI: 10.4161/biom.19835

Andrea Spinelli, Bruna Vinci, Annalisa Tirella, Marco Matteucci, Luna Gargani, Arti Ahluwalia, Claudio Domenici, Eugenio Picano, Piero Chiarelli

引用次数: 0

Maintenance of α(1)-antitrypsin activity by means of co-application of hypochlorous acid-scavengers in vitro and in the supernatant of polymorphonuclear leukocytes: as a basis for a new drug delivery approach. 通过在体外和多形核白细胞上清中共同应用次氯酸清除剂维持α(1)-抗胰蛋白酶活性:作为一种新的药物递送方法的基础。

Biomatter Pub Date : 2012-01-01 DOI: 10.4161/biom.19190

Maria Schönberg, Uta Reibetanz, Sophie Rathmann, Jacqueline Lessig

{"title":"Maintenance of α(1)-antitrypsin activity by means of co-application of hypochlorous acid-scavengers in vitro and in the supernatant of polymorphonuclear leukocytes: as a basis for a new drug delivery approach.","authors":"Maria Schönberg, Uta Reibetanz, Sophie Rathmann, Jacqueline Lessig","doi":"10.4161/biom.19190","DOIUrl":"https://doi.org/10.4161/biom.19190","url":null,"abstract":"Tissue destruction, pain and loss of function in chronically inflamed tissues can result from noxious agents released from myeloperoxidase (MPO) and its highly reactive product hypochlorous acid (HOCl) or proteases such as neutrophil elastase (NE). Currently there exists a high demand for medications that provide gentle treatments, free from side effects inherent in those prescribed today. One method to circumvent side effects is through the use of locally applied drug delivery. In contrast to systemic therapy, the main advantages of transport systems are the low dosages of drug with a time-controlled delivery. The aim of this study was to ascertain interactions of NE and its inhibitor α(1)-antitrypsin (AT), the influence of hypochlorous acid (HOCl), as well as its scavengers, in order to define an effective mixture of drugs acting in a synergistic way which can be applied by means of drug delivery systems. These investigations determine the effective amounts of AT/HOCl-scavengers that drug mixtures need for delivery under inflammatory conditions in order to prevent tissue damage. AT was shown to inhibit NE in a dose-dependent manner, whereas a physiological concentration of 1.14 µM AT caused a significant NE inhibition (78%, pH 7.5). The concomitant existence of MPO/HOCl inactivated AT in a dose-dependent manner as well. To regain AT efficacy, HOCl-scavengers, such as L-methionine, α-aminosalicylic acid and cefoperazone were additionally applied. Finally, AT was assembled as surface layer onto layer-by-layer biopolymer-coated microcarriers and carrier phagocytosis by polymorphonuclear leukocytes could be shown.","PeriodicalId":8891,"journal":{"name":"Biomatter","volume":"2 1","pages":"24-36"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/biom.19190","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31318838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Resolving the CaP-bone interface: a review of discoveries with light and electron microscopy. 解析CaP-bone界面:光镜和电子显微镜发现综述。

Biomatter Pub Date : 2012-01-01 DOI: 10.4161/biom.20062

Kathryn Grandfield, Anders Palmquist, Håkan Engqvist, Peter Thomsen

引用次数: 8

Regenerative therapy and tissue engineering for the treatment of end-stage cardiac failure: new developments and challenges. 再生疗法和组织工程治疗终末期心力衰竭:新进展和挑战。

Biomatter Pub Date : 2012-01-01 DOI: 10.4161/biom.19429

G T Finosh, Muthu Jayabalan

引用次数: 44

Implanted neonatal human dermal fibroblasts influence the recruitment of endothelial cells in mice. 植入新生人真皮成纤维细胞对小鼠内皮细胞募集的影响。

Biomatter Pub Date : 2012-01-01 DOI: 10.4161/biom.20063

Susana G Guerreiro, Christoph Brochhausen, Rita Negrão, Mário A Barbosa, Ronald E Unger, C James Kirkpatrick, Raquel Soares, Pedro L Granja

{"title":"Implanted neonatal human dermal fibroblasts influence the recruitment of endothelial cells in mice.","authors":"Susana G Guerreiro, Christoph Brochhausen, Rita Negrão, Mário A Barbosa, Ronald E Unger, C James Kirkpatrick, Raquel Soares, Pedro L Granja","doi":"10.4161/biom.20063","DOIUrl":"https://doi.org/10.4161/biom.20063","url":null,"abstract":"The vascularization of new tissue within a reasonable time is a crucial prerequisite for the success of different cell- and material-based strategies. Considering that angiogenesis is a multi-step process involving humoral and cellular regulatory components, only in vivo assays provide the adequate information about vessel formation and the recruitment of endothelial cells. The present study aimed to investigate if neonatal human dermal fibroblasts could influence in vivo neovascularization. Results obtained showed that fibroblasts were able to recruit endothelial cells to vascularize the implanted matrix, which was further colonized by murine functional blood vessels after one week. The vessels exhibited higher levels of hemoglobin, compared with the control matrix, implanted without fibroblasts, in which no vessel formation could be observed. No significant differences were detected in systemic inflammation. The presence of vessels originated from the host vasculature suggested that host vascular response was involved, which constitutes a fundamental aspect in the process of neovascularization. Fibroblasts implanted within matrigel increased the presence of endothelial cells with positive staining for CD31 and for CD34 and the production of collagen influencing the angiogenic process and promoting the formation of microvessels. New strategies in tissue engineering could be delineated with improved angiogenesis using neonatal fibroblasts.","PeriodicalId":8891,"journal":{"name":"Biomatter","volume":"2 1","pages":"43-52"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/biom.20063","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31318238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Development of affinity-based delivery of NGF from a chondroitin sulfate biomaterial. 硫酸软骨素生物材料亲和递送NGF的研究进展。

Biomatter Pub Date : 2011-10-01 DOI: 10.4161/biom.18791

Karen Chao Butterfield, Aaron W Conovaloff, Alyssa Panitch

引用次数: 25

Factorial design-based development of measlamine microspheres for colonic delivery. 基于因子设计的甲胺微球结肠给药研究。

Biomatter Pub Date : 2011-10-01 DOI: 10.4161/biom.18461

Vikas Jain, Dayal Prasad, Deepika Jain, Santosh Kumar Mishra, Ranjit Singh

{"title":"Factorial design-based development of measlamine microspheres for colonic delivery.","authors":"Vikas Jain, Dayal Prasad, Deepika Jain, Santosh Kumar Mishra, Ranjit Singh","doi":"10.4161/biom.18461","DOIUrl":"https://doi.org/10.4161/biom.18461","url":null,"abstract":"For treating colonic diseases, conventional oral drug delivery systems are not effective, as they fail to reach the appropriate site of action. Thus, there is a need to develop effective and safe therapy for the treatment of colonic disorders. The aim of the present study was to design a colon-specific delivery system for an anti-inflammatory drug, mesalamine, with minimal degradation and optimum delivery of the drug with relatively higher local concentration, which may provide more effective therapy for inflammatory bowel disease including Crohn disease and ulcerative colitis. Factorial designs (four factors and two levels) for eudragit S-100 (pH-dependent polymer)-coated, pectin (natural polysaccharides)-based microspheres of mesalamine were constructed and conducted in a fully randomized manner to study all possible combinations. Based on the desirability function formulation, F14 was found to be the best formulation. The overall desirability coefficient of formulation F14 was found to be 0.825. The formulation F14 was subjected to in vitro release studies, and the results were evaluated kinetically and statistically. The microspheres started releasing the drug at the beginning of 7th hour, which corresponds to the arrival time at proximal colon. The cumulative percent drug release for formulation F14 at the end of 16 h was found to be 98%. The release kinetics showed that the release followed the Higuchi model, and the main mechanism of drug release was diffusion. The study presents a new approach for colon-specific drug delivery.","PeriodicalId":8891,"journal":{"name":"Biomatter","volume":"1 2","pages":"182-8"},"PeriodicalIF":0.0,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/biom.18461","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31317447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Designing polysaccharide-based antibacterial biomaterials for wound healing applications. 设计用于伤口愈合的多糖类抗菌生物材料。

Biomatter Pub Date : 2011-10-01 DOI: 10.4161/biom.19005

Amita Chhatri, Jaya Bajpai, A K Bajpai

引用次数: 32