Biology, Medicine, & Natural Product Chemistry最新文献_第7页

Cover, Editorial Board, Guidance for Authors, & Table of Contents 封面，编辑委员会，作者指南，目录

Biology, Medicine, & Natural Product Chemistry Pub Date : 2019-10-31 DOI: 10.14421/BIOMEDICH.2019.82.%P

Muhammad Ja’far Luthfi

引用次数: 0

Liver Profile of Atazanavir/Ritonavir in Pregnant Albino Rats 阿扎那韦/利托那韦对妊娠白化大鼠肝脏的影响

Biology, Medicine, & Natural Product Chemistry Pub Date : 2019-10-31 DOI: 10.14421/biomedich.2019.82.47-52

Elias Adikwu, J. Kemelayefa, Winifred Ocheiga

{"title":"Liver Profile of Atazanavir/Ritonavir in Pregnant Albino Rats","authors":"Elias Adikwu, J. Kemelayefa, Winifred Ocheiga","doi":"10.14421/biomedich.2019.82.47-52","DOIUrl":"https://doi.org/10.14421/biomedich.2019.82.47-52","url":null,"abstract":"Medication use during pregnancy is challenging due to the occurrence of maternal or fetal toxicities. Atazanavir/ritonavir (ATV/r) has hepatotoxic potential hence; use in pregnant patients living with human immunodeficiency virus may cause maternal hepatotoxicity. This study assessed the liver profile of ATV/r in pregnant albino rats. Thirty pregnant albino rats randomized into groups were orally treated daily with ATV/r (4.28/1.43 mg/kg-34.3/11.4 mg/kg) for 16 days. After treatment, the rats were weighed and sacrificed. Blood samples were collected and examined for serum biochemical parameters. Liver samples were weighed and assessed for biochemical and histological changes. Body and liver weights were normal (p>0.05) in ATV/r-treated pregnant rats when compared to control. Serum total cholesterol, triglyceride, low density lipoprotein cholesterol and blood glucose levels were significantly (p<0.01) elevated whereas high density lipoprotein cholesterol level was significantly (p<0.01) decreased in rats treated with ATV/r (34.3/11.4 mg/kg) when compared to control. Liver and serum aminotransferases, alkaline phosphatase, gamma-glutamyl transferase, lactate dehydrogenase, total bilirubin, and conjugated bilirubin levels were significantly increased in a dose-dependent fashion in rats treated with ATV/r; 8.57/2.86 mg/kg (p<0.05), 17.1/5.72 mg/kg (p<0.01) and 34.3/11.4 mg/kg (p<0.001) when compared to control. Liver superoxide dismutase, catalase, glutathione and glutathione peroxidase levels were significantly decreased whereas malondialdehyde levels were significantly increased in a dose-dependent fashion in rats treated with ATV/r; 8.57/2.86 mg/kg (p<0.05), 17.1/5.72 mg/kg (p<0.01) and 34.3/11.4 mg/kg (p<0.001) when compared to control. Necrotic hepatocytes were observed at higher doses of ATV/r. ATV/r may not be hepatotoxic in pregnant women living with HIV at the clinical dose.","PeriodicalId":8882,"journal":{"name":"Biology, Medicine, & Natural Product Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77672013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anatomical and Histological Study of Shark (Carcharhinus sorrah) Kidney 鲨鱼肾脏的解剖组织学研究

Biology, Medicine, & Natural Product Chemistry Pub Date : 2019-10-31 DOI: 10.14421/biomedich.2019.82.37-40

Hikmah Supriyati, Rakhmiyati Rakhmiyati, Muhammad Ja’far Luthfi

引用次数: 0

The Effects of Wet Cupping Therapy in Blood Pressure, Glucose, Uric Acid and Total Cholesterol Levels 湿罐疗法对血压、血糖、尿酸和总胆固醇水平的影响

Biology, Medicine, & Natural Product Chemistry Pub Date : 2019-10-31 DOI: 10.14421/biomedich.2019.82.33-36

Sutriyono Sutriyono, Muhammad Rodham Robbina, M. Z. Ndii

{"title":"The Effects of Wet Cupping Therapy in Blood Pressure, Glucose, Uric Acid and Total Cholesterol Levels","authors":"Sutriyono Sutriyono, Muhammad Rodham Robbina, M. Z. Ndii","doi":"10.14421/biomedich.2019.82.33-36","DOIUrl":"https://doi.org/10.14421/biomedich.2019.82.33-36","url":null,"abstract":"Wet cupping therapy is a simple, cheap, and effective alternative medical treatment. In China wet cupping therapy become a formal treatment in the hospital. 14 centuries ago Prophet Muhammad implemented wet cupping therapy or hejamah as a curing treatment and become sunnah for muslim. Today in a modern world, many studies proved the advantages of wet cupping therapy, which is a sunnah since 14 centuries ago. The aims of this study were to measure the effects of wet cupping therapy treatment on blood pressure, blood glucose, uric acid, and total cholesterol level. 21 participants were treated with hejamah/wet cupping, blood pressure and blood samples were collected from all the participants one week before and one week after hejamah. Blood pressure, either systolic or diastolic was significantly decreased following therapy (p|<|0.05), blood glucose had a slight decreased but not significant (p|>|0.05). uric acid and total cholesterol level was also significantly decreased following therapy (p|<|0.05). With only just one time treatment, hejamah had a significant effects on blood pressure, uric acid and total cholesterol level, that is proved the advantages of hejamah.","PeriodicalId":8882,"journal":{"name":"Biology, Medicine, & Natural Product Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90337000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Hepatotoxic Assessment of Tramadol-Diclofenac Use: A Study in a Rat Model 曲马多-双氯芬酸肝毒性评价:大鼠模型研究

Biology, Medicine, & Natural Product Chemistry Pub Date : 2019-10-31 DOI: 10.14421/biomedich.2019.82.41-45

Elias Adikwu, E. Nelson

{"title":"Hepatotoxic Assessment of Tramadol-Diclofenac Use: A Study in a Rat Model","authors":"Elias Adikwu, E. Nelson","doi":"10.14421/biomedich.2019.82.41-45","DOIUrl":"https://doi.org/10.14421/biomedich.2019.82.41-45","url":null,"abstract":"The concurrent use of tramadol and diclofenac may increase hepatotoxic risk due to their individual hepatotoxic effects. This study assessed the hepatotoxic effect of tramadol-diclofenac administration in albino rats. Twenty-four adult male albino rats (200-220g) randomized into four groups were orally administered with tramadol (12mg/kg/day), diclofenac (6mg/kg/day) and tramadol-diclofenac for 14 days respectively. The rats were anesthetized, blood samples were collected and evaluated for serum liver function and lipid parameters. Liver samples were weighed and evaluated for biochemical parameters and histology. The effects of tramadol-diclofenac on the body and liver weights did not differ significantly (p>0.05) when compared to control. Also, effects were not significant (p>0.05) on blood glucose, and serum cholesterol, triglyceride, low and high density lipoprotein cholesterol levels when compared to control. Liver and serum levels of aminotransferases, alkaline phosphatase, lactate dehydrogenase, gamma–glutamyl transferase, conjugated bilirubin and total bilirubin increased significantly in rats treated with tramadol (p<0.05), diclofenac (p<0.01) and tramadol-diclofenac (p<0.001) when compared to control. Furthermore, significant decreases in liver catalase, glutathione, superoxide dismutase, glutathione peroxidase levels with significant increases in malondialdehyde levels occurred in rats treated with tramadol (p<0.05), diclofenac (p<0.01) and tramadol-diclofenac (p<0.001) when compared to control. Hepatocyte necrosis was observed in rats treated with tramadol-diclofenac. Tramadol-diclofenac may increase hepatotoxic risk at doses used for this study.","PeriodicalId":8882,"journal":{"name":"Biology, Medicine, & Natural Product Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82479647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Approaches for Detection Fluorescent-Labeled by Cellvizio Lab System on Hippocampal CA1 Region Cellvizio实验室系统在海马CA1区荧光标记检测的新方法

Biology, Medicine, & Natural Product Chemistry Pub Date : 2019-05-01 DOI: 10.14421/biomedich.2019.81.1-6

Asma Ulhusna Shaimi, W. Aasim, H. Abdullah, T. S. Choon, A. Wei, Z. Ismail

{"title":"Novel Approaches for Detection Fluorescent-Labeled by Cellvizio Lab System on Hippocampal CA1 Region","authors":"Asma Ulhusna Shaimi, W. Aasim, H. Abdullah, T. S. Choon, A. Wei, Z. Ismail","doi":"10.14421/biomedich.2019.81.1-6","DOIUrl":"https://doi.org/10.14421/biomedich.2019.81.1-6","url":null,"abstract":"Neurosteroids have been identified in the 1981. Dehydroepiandrosterone sulphate (DHEAS) is one of the vital neurosteroids that de novo synthesized in the nervous system from cholesterol precursor (Baulieu & Robel, 1998). The aim of the study is to develop a method for fluorescence labelling. Alexa Fluor 488 dye with DHEAS antibody can binds the DHEAS antibody in the rat brain monitored by Cellvizio Lab System. DHEAS antibody (IgG isotype antibodies) was fluorescently conjugated by an amine-reactive compound, Alexa Fluor 5-SDP ester 488 dye. The resultant Alexa Fluor 488-conjugated antibodies were collected and analyzed by UV-Vis spectrophotometer instrument. The absorbance of the protein-dye conjugate at 280 nm and 494 nm were measured. Then, the degree of labeling (DOL) was calculated to achieve the desired results. Fluorescence labelling were carried out into the CA1 region of hippocampus Sprague-Dawley rat. We reported that the conjugation was successful. Optimal labeling depending on degree of labeling (DOL) needs some necessity to achieve and effective binding to the target neurosteroid, DHEAS. Cellvizio Lab system connected with Fiber Fluorescence Microscopy (FFM) probe is presented as a new approach in real-time imaging of DHEAS. In conclusion, we have developed a new method of DHEAS-Alexa Fluor fluorescence labelling to visualize and evaluate the changes of DHEAS fluorescence level in the rat hippocampus. This novel approach as a diagnostic tool and can be used to better understand the mechanisms and functions of DHEAS and other neurosteroids in future research.","PeriodicalId":8882,"journal":{"name":"Biology, Medicine, & Natural Product Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75360688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mathematical Model of Cervical Cancer Treatment Using Chemotherapy Drug 化疗药物治疗宫颈癌的数学模型

Biology, Medicine, & Natural Product Chemistry Pub Date : 2019-05-01 DOI: 10.14421/biomedich.2019.81.11-15

M. Murtono, M. Z. Ndii, S. Sugiyanto

引用次数: 5

Statistical Analysis of Habbatussauda’s Benefits for Health (Blood Pressure, Glucose and Uric Acid) 哈巴杜龙有益健康(血压、血糖和尿酸)的统计分析

Biology, Medicine, & Natural Product Chemistry Pub Date : 2019-05-01 DOI: 10.14421/biomedich.2019.81.7-10

S. Sugiyanto, Luqyana Khalda, M. Z. Ndii

引用次数: 0

A Simple Method for Clearing and Staining Specimens for The Demonstration of Animal Skeleton 动物骨骼展示标本的简单清理染色方法

Biology, Medicine, & Natural Product Chemistry Pub Date : 2019-05-01 DOI: 10.14421/biomedich.2019.81.17-21

Muhammad Ja’far Luthfi, Nyoman Puniawati Soesilo

引用次数: 0

Echinoderms Diversity and Abundance in Gunung Kidul Beach Yogyakarta 日惹Gunung Kidul海滩棘皮动物的多样性和丰度

Biology, Medicine, & Natural Product Chemistry Pub Date : 2019-05-01 DOI: 10.14421/biomedich.2019.81.23-26

Hikmah Supriyati, Setyawati Dwi K, Ahzami Ahzami, Farhani Sodiq, Septiana Khoiriyah

{"title":"Echinoderms Diversity and Abundance in Gunung Kidul Beach Yogyakarta","authors":"Hikmah Supriyati, Setyawati Dwi K, Ahzami Ahzami, Farhani Sodiq, Septiana Khoiriyah","doi":"10.14421/biomedich.2019.81.23-26","DOIUrl":"https://doi.org/10.14421/biomedich.2019.81.23-26","url":null,"abstract":"Echinoderms are one of marine invertebrate animals that have an important role, as a recycle of nutrients in the functioning of ecosystems. Echinoderms usually appear in the intertidal zone of the coast. The existence of echinoderms in the intertidal zone is affected by external and internal factors. This study aims to determine the level of abundance and diversity of Echinoderms in the intertidal zone in the Krakal and Drini coastal areas, Gunung Kidul, Yogyakarta. Data were collected using transect method. The main transect along 50 meters was divided into 5 sampling points, and the distance between sampling points was 10 meters. After that, each point was taken for 3 observation plots with an area of 1x1 meters and the distance between plots is 5 meters. The results showed that, the diversity index on the krakal coast had a higher value (0.139) when compared to the Drini coast (0.129). Calculation of echinonderm abundance index shows that the Drini beach location has a high abundance value when compared to the abundance value at the Krakal beach loc ation. The parameter measurement results can be seen that both the pH and temperature parameters on both the Krakal and Drini coast have values that correspond to the optimum parameter in the waters.","PeriodicalId":8882,"journal":{"name":"Biology, Medicine, & Natural Product Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86212264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2