Biology, Medicine, & Natural Product Chemistry最新文献_第8页

Diversity of Angiospermae Plant Class Liliopsida in Mount Nglanggeran 文章标题兰岗仁山被子植物百合科植物的多样性

Biology, Medicine, & Natural Product Chemistry Pub Date : 2018-10-31 DOI: 10.14421/BIOMEDICH.2018.72.45-49

Bayu Setya Aji Nugraha, W. Widodo, Rendi Yuntara, Normalita Normalita

引用次数: 1

Degradation Study of Biodegradable Plastic Using Nata De Coco as A Filler 椰果为填料的可生物降解塑料降解研究

Biology, Medicine, & Natural Product Chemistry Pub Date : 2018-10-31 DOI: 10.14421/BIOMEDICH.2018.72.33-38

Tiara Nur Elfiana, A. Fitria, E. Sedyadi, S. Y. Prabawati, Irwan Nugraha

{"title":"Degradation Study of Biodegradable Plastic Using Nata De Coco as A Filler","authors":"Tiara Nur Elfiana, A. Fitria, E. Sedyadi, S. Y. Prabawati, Irwan Nugraha","doi":"10.14421/BIOMEDICH.2018.72.33-38","DOIUrl":"https://doi.org/10.14421/BIOMEDICH.2018.72.33-38","url":null,"abstract":"Starch is known as a biodegradable raw material that can be degraded by bacteria and microorganisms in the soil. Starch has cellulose which is kind of plant cellulose. This study shows the biodegradation rates of plastic made from Ganyong Canna (Canna edulis Kerr) as a cellulose source which is added with nata de coco as a filler. The biodegradable plastic functional group was confirmed by using FITR. The results show that the O-H group of Ganyong Canna (Canna edulis Kerr) biodegradable plastic is located at wave number 3298.03 cm-1 and shifted to 3290.32 cm-1 after addition of nata de coco. The C-H bonds functional groups in Canna biodegradable plastics and nata de coco plastics are at wave numbers 2920.01 cm-1 and 2916.16 cm-1. While the C-O bonds functional groups in biodegradable starch plastics and nata de coco is shown at wave numbers 995.05 cm-1. The mechanical properties of biodegradable plastics testing are thickness, tensile strength, and elongation based on the ASTM method. The thickness is about 0.1005 mm, the tensile strength of biodegradable plastic is 4,3244 MPa and the elongation value range about 13.9639% while the WVTR range about 14.20 g/m² hours. The results show that the increase of the plastic degradation made from nata de coco occurs between 5% - 38% per days. It is faster than the plastic made from pure Ganyong Canna (Canna edulis Kerr) starch. These results indicate that nata de coco could be added in biodegradable plastic on packaging materials for better degradation.","PeriodicalId":8882,"journal":{"name":"Biology, Medicine, & Natural Product Chemistry","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73449837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Alizarin Red S-Alcian Blue Staining for Regenerated tail of Common House Gecko (Hemidactylus frenatus) 茜素红s -阿利新蓝染色法对爪壁虎再生尾的影响

Biology, Medicine, & Natural Product Chemistry Pub Date : 2018-10-31 DOI: 10.14421/BIOMEDICH.2018.72.57-59

Rakhmiyati Rakhmiyati, Muhammad Ja’far Luthfi

引用次数: 3

Link of Nasopharyngeal Carcinoma and Epstein-Barr Virus 鼻咽癌与eb病毒的关系

Biology, Medicine, & Natural Product Chemistry Pub Date : 2018-10-31 DOI: 10.14421/BIOMEDICH.2018.72.51-55

S. Sugiyanto, L. Aryati, F. A. Kusumo, M. Hardianti

引用次数: 1

Comparative Anatomy of Labyrinth and Gill of Catfish (Clarias gariepinus) (Burchell, 1822) and Snakehead Fish (Channa striata) (Bloch, 1793) 鲶鱼(Clarias gariepinus) (Burchell, 1822)和黑头鱼(Channa striata) (Bloch, 1793)迷宫和鳃的比较解剖

Biology, Medicine, & Natural Product Chemistry Pub Date : 2018-10-31 DOI: 10.14421/BIOMEDICH.2018.72.39-43

Ina Karlina, Muhammad Ja’far Luthfi

引用次数: 0

Cover, Editorial Board, Guidance for Authors 封面、编委会、作者指南

Biology, Medicine, & Natural Product Chemistry Pub Date : 2018-10-30 DOI: 10.14421/BIOMEDICH.2018.72.%P

R. Riyanto

引用次数: 0

Stability of T-DNA Integration in Phalaenopsis “Sogo Vivien” Transgenic Orchid Carrying 35S::Gal4::AtRKD4::GR 携带35S:：Gal4:：AtRKD4:：GR的蝴蝶兰“Sogo Vivien”转基因兰花T-DNA整合的稳定性

Biology, Medicine, & Natural Product Chemistry Pub Date : 2018-04-30 DOI: 10.14421/BIOMEDICH.2018.71.5-13

E. Semiarti, E. Mursyanti, A. Suyoko, Faiza Senja Widya Perdana, Catharina Tri Widyastuti, A. N. Subchan

{"title":"Stability of T-DNA Integration in Phalaenopsis “Sogo Vivien” Transgenic Orchid Carrying 35S::Gal4::AtRKD4::GR","authors":"E. Semiarti, E. Mursyanti, A. Suyoko, Faiza Senja Widya Perdana, Catharina Tri Widyastuti, A. N. Subchan","doi":"10.14421/BIOMEDICH.2018.71.5-13","DOIUrl":"https://doi.org/10.14421/BIOMEDICH.2018.71.5-13","url":null,"abstract":"Orchid is an elegant ornamental plant and favoured by the society. Phalaenopsis \"Sogo vivien\" is a mini-sized orchid with an interesting white-striped purple petals. This study was aimed to analyze the stability of the integration of embryonic gene carrier T-DNA from Arabidobsis AtRKD4 into the P. \"Sogo vivien\" genome produced in 2016. The study was conducted in 3 stages: 1) Transgenic plant phenotype analysis (1 year old); 2) Examination of T-DNA integration in orchid genotypes using PCR. 3) Analysis of transgenic plant leaf explants’ ability to produce somatic embryo in vitro. In vitro cultures were performed on the base medium of New Phalaenopsis (NP), plus various concentrations of TDZ (0, 1, 2 mg.L -1 ) and IBA (0, 1, 2 mg.L -1 ) or without TDZ and IBA as controls. The transgenic Phalaenopsis ‘Sogo vivien’ were transferred to pot mediums via ex vitro with two treatments: the first leaves were cut as explants for in vitro culture, and the plants were transferred to the mixture of fern medium with shavings of bark. The integration of T-DNA in the genome was detected by DNA genome amplification from the second leaves using the AtRKD4 gene primers and the POH1 gene. The results showed that the highest number of somatic embryo (SE) propagules or protocorm like bodies (PLBs) amounted to 27 were derived from transgenic plant # 2 cultured on NP + 2 mg.L -1 TDZ +1 mg.L -1 IBA medium. The presence of AtRKD4 transgenes were detected with the amplification of 380 bp of the RKD4 gene from the genome of transgenic plant # 2 by using PCR. There were 2 out of 15 plants that positively carry the AtRKD4 gene and produce SE. Thus, the stability of the AtRKD4 carrier T-DNA integration in the genomes of transgenic plants was 13.3%.","PeriodicalId":8882,"journal":{"name":"Biology, Medicine, & Natural Product Chemistry","volume":"7 1","pages":"5-13"},"PeriodicalIF":0.0,"publicationDate":"2018-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48811323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Quercetin: the bioactive compound from Allium cepa L. as anti-inflammation based on in silico screening 槲皮素：洋葱中的一种具有抗炎活性的化合物

Biology, Medicine, & Natural Product Chemistry Pub Date : 2018-04-30 DOI: 10.14421/BIOMEDICH.2018.71.27-31

M. Amin, K. Putra, I. F. Amin, N. Earlia, Dina Maulina, B. Lukiati, U. Lestari

{"title":"Quercetin: the bioactive compound from Allium cepa L. as anti-inflammation based on in silico screening","authors":"M. Amin, K. Putra, I. F. Amin, N. Earlia, Dina Maulina, B. Lukiati, U. Lestari","doi":"10.14421/BIOMEDICH.2018.71.27-31","DOIUrl":"https://doi.org/10.14421/BIOMEDICH.2018.71.27-31","url":null,"abstract":"Inflammation is a tissue injury that occurs due to physical trauma or microbiological substances that involve the activities of many cell types. Inflammation can be prevented using the natural medicines from Allium cepa L. Quercetin is one of the bioactive compounds found in Allium cepa L and has been reported to have anti-inflammatory activity. The natural medicines have been used to minimize non-steroidal anti-inflammatory drugs. This study aims to investigated the modeling structures and the protein receptor from quecertin in inflammation mechanism and their optimization of the effectiveness in the human body. The bioinformatics tools used in this study are the database of quercetin compounds, Pubchem and Swis Target Prediction protein prediction databases, PyRx 0.8 molecular docking software, ligand docking, and binding site analysis with PyMOL and LigPlus software. The results from in silico show that quercetin compounds can interact with Muscleblind-like protein 1 target protein with a Binding Affinity minus value which is not much different from the dexamethasone compound. Dexamethason is a standart because it is a corticosteroid drug that can be used as an anti-inflammatory to reduce inflammation, allergic reactions, arthritis and other inflammatory diseases.","PeriodicalId":8882,"journal":{"name":"Biology, Medicine, & Natural Product Chemistry","volume":"7 1","pages":"27-31"},"PeriodicalIF":0.0,"publicationDate":"2018-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46295150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Leukocytes Description of Mudskipper (Periophthalmodon schlosseri) of Barito River Estuary, Desa Tanipah, Kalimantan Selatan

Biology, Medicine, & Natural Product Chemistry Pub Date : 2018-04-30 DOI: 10.14421/BIOMEDICH.2018.71.1-4

Heri Santoso, Hidayaturrahmah Hidayaturrahmah, Muhamat Muhamat

{"title":"Leukocytes Description of Mudskipper (Periophthalmodon schlosseri) of Barito River Estuary, Desa Tanipah, Kalimantan Selatan","authors":"Heri Santoso, Hidayaturrahmah Hidayaturrahmah, Muhamat Muhamat","doi":"10.14421/BIOMEDICH.2018.71.1-4","DOIUrl":"https://doi.org/10.14421/BIOMEDICH.2018.71.1-4","url":null,"abstract":"Leukocytes have an important role in driving away infections from pathogen microorganism by phagocytosis together with macrophages. The aim of this research was to analyze the leukocytes’ differentiation (eosinophil, basophil, neutrophil, lymphocytes, and monocytes) of mudskipper‘s blood (Periophthalmodon schlosseri ). Samples were taken from desa Tanipah Kalimantan Selatan. The sampling locations were decided by purposive or taking the data intentionally according to the consideration of mudskipper‘s location which is not homogenous. The data collection were conducted using Line Transect which means the sample was taken according to how many encounters can be taken. There were 15 mudskippers taken from Desa Tanipah used in this research. The parameter observed wasthe differentiation of leukocytes which consists of the percentages of monocytes, lymphocytes, eosinophil, basophil, and heterophil. The result showed that the percentage of lymphocytes in mudskippers is 62 + 4,1% and monocytes 24,7 + 0,8%, and the neutrophil is 0,5 + 0,1% and eosinophil 0,6 + 0,1% and no basophils were found. According to the result, it can be concluded that lymphocytesis the most dominant one amongst others.","PeriodicalId":8882,"journal":{"name":"Biology, Medicine, & Natural Product Chemistry","volume":"7 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2018-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44578819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of detergent and CTAB method for isolation of DNA from Salak ( Salacca zalacca (Gaert.) Voss. ‘Pondoh’) 洗涤剂和CTAB法分离萨拉克（Salaca zalacca（Gaert.）Voss.）DNA的比较Pondoh'）

Biology, Medicine, & Natural Product Chemistry Pub Date : 2018-04-30 DOI: 10.14421/BIOMEDICH.2018.71.15-20

Namira Nur Arfa, B. Daryono, R. Reflinur

{"title":"Comparison of detergent and CTAB method for isolation of DNA from Salak ( Salacca zalacca (Gaert.) Voss. ‘Pondoh’)","authors":"Namira Nur Arfa, B. Daryono, R. Reflinur","doi":"10.14421/BIOMEDICH.2018.71.15-20","DOIUrl":"https://doi.org/10.14421/BIOMEDICH.2018.71.15-20","url":null,"abstract":"This study conducted in Laboratory of Molecular Biology, Balai Besar Bioteknologi dan Sumberdaya Genetik Pertanian (BB- Biogen) Bogor. The aims of this study are to determine and comparing the quantity, quality and the efficiency of DNA isolation result using detergent method and CTAB method. The parameters observed in this study are the value of DNA concentration, purity, and visualization result using gel electrophoresis. The samples are the leaves of Salak ‘Pondoh’ ( Salacca zalacca (Gaert.) Voss.). Detergent method is a method which was developed by Faculty of Biology UGM, it has simple method and relatively affordable cost. Meanwhile, CTAB method is one of the commonly used method s of DNA isolation protocol with relatively expensive cost. Detergent method used detergent in the cell wall separation and protein removal in the sample. The CTAB method used Cetyltrimethyl ammonium bromide (CTAB) for cell membrane separation in the sample. The research methods included DNA isolation with detergent and CTAB methods, PCR analysis and electrophoresis. Data analysis was done quantitatively using spectrophotometric method and qualitative used electrophoresis method. The result of the study showed that DNA isolation using CTAB method showed higher purity compared with detergent method with the purity values ranging from 1,3- 1,4 . Meanwhile, the concentration of DNA in the detergent method was higher than that of CTAB with the highest concentration of 1730 µg/ml. There is no difference between the quality of genomic DNA isolated by CTAB and detergent methods.","PeriodicalId":8882,"journal":{"name":"Biology, Medicine, & Natural Product Chemistry","volume":"7 1","pages":"15-20"},"PeriodicalIF":0.0,"publicationDate":"2018-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41474177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1