Medical laser application : international journal for laser treatment and research最新文献_第8页

25 years Laser- und Medizin-Technologie GmbH, Berlin 柏林激光药发公司25年授权

Medical laser application : international journal for laser treatment and research Pub Date : 2010-08-01 DOI: 10.1016/j.mla.2010.05.005

引用次数: 0

Development of a confocal and two-photon endomicroscope – Preliminary results of qualitative evaluation 共聚焦双光子内窥镜的研制——定性评价的初步结果

Medical laser application : international journal for laser treatment and research Pub Date : 2010-08-01 DOI: 10.1016/j.mla.2010.03.001

Katharina Thomsen, Richard Meier, Herbert Stepp, Ronald Sroka

{"title":"Development of a confocal and two-photon endomicroscope – Preliminary results of qualitative evaluation","authors":"Katharina Thomsen, Richard Meier, Herbert Stepp, Ronald Sroka","doi":"10.1016/j.mla.2010.03.001","DOIUrl":"10.1016/j.mla.2010.03.001","url":null,"abstract":"<div><p>Optical methods, particularly confocal microscopy (CFM) and two-photon microscopy (TPM), have the potential to support or even replace the traditional histological diagnosis of cell-altering diseases. The high axial and lateral resolution of these techniques enables 3D imaging of even intracellular details. Using optical fibers, these systems can be miniaturized to a size sufficiently small to access the surface cells of a hollow organ through the working channel of an endoscope. In contrast to CFM, the TPM technique employs pulsed laser sources in order to nonlinearly stimulated fluorescence confined to the focal spot. Especially in cases of high power density in the optical fiber, and in addition to the dispersion effects, a nonlinear interaction of the light pulses with the fiber material will distort the pulses and therefore reduce the detectable signal. This paper presents the development of a confocal and a two-photon endomicroscope. In the experimental set-up, both a fs- and a cw-laser were coupled into a fiber bundle in order to compare qualitative aspects of confocal and two-photon imaging. It was possible to resolve cellular structure with a high axial resolution using this system. However, further developments are needed to improve the efficiency of the fluorescence excitation.</p></div>","PeriodicalId":88584,"journal":{"name":"Medical laser application : international journal for laser treatment and research","volume":"25 3","pages":"Pages 166-172"},"PeriodicalIF":0.0,"publicationDate":"2010-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mla.2010.03.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54920721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A human skin explant model to study molecular changes in response to fractional photothermolysis: Spatio-temporal expression of HSP70 利用人体皮肤外植体模型研究部分光热分解反应的分子变化:HSP70的时空表达

Medical laser application : international journal for laser treatment and research Pub Date : 2010-08-01 DOI: 10.1016/j.mla.2009.12.002

Doris Helbig, Marc Bodendorf, Ulf Anderegg, Jan C. Simon, Uwe Paasch

{"title":"A human skin explant model to study molecular changes in response to fractional photothermolysis: Spatio-temporal expression of HSP70","authors":"Doris Helbig, Marc Bodendorf, Ulf Anderegg, Jan C. Simon, Uwe Paasch","doi":"10.1016/j.mla.2009.12.002","DOIUrl":"10.1016/j.mla.2009.12.002","url":null,"abstract":"<div><h3>Background and objectives</h3><p>Ablative fractional photothermolysis (AFP) is a new concept to treat aged skin. Despite growing clinical experience, little is known about the molecular changes induced over time by AFP in the targeted skin compartments. This is due, in part, to difficulties in obtaining multiple skin biopsies from AFP-treated individuals. In an attempt to circumvent these limitations, a human skin explant model was designed. In order to test its suitability as a model to study molecular changes induced by AFP, the spatio-temporal expression of heat shock protein 70 (HSP70) has been investigated in response to AFP at different pulse energies.</p></div><div><h3>Materials and methods</h3><p>AFP was performed using a scanned 250μm CO<sub>2</sub>-laser beam. The ablative single pulse energies were set to 50, 64 and 300mJ at 150 ablation zones per cm<sup>2</sup>. The immediate response to AFP, as well as a response 1, 3 or 7 days after AFP, was studied; untreated skin explants served as a control. Additionally, intradermal injections of transforming growth factor β (TGF-β) on day 0 served as a positive control for HSP70 induction in non-laser-treated samples, cultivated over 7 days. A routine pathology workup and immunohistochemistry (HSP70) was performed in all untreated (control group and positive control samples) and AFP-treated samples. In summary, a total of 245 samples were investigated.</p></div><div><h3>Results</h3><p>In this explant model, HSP70 showed a clear time-dependent induction by AFP with expression profile being most prominent in the epidermis, a marked up-regulation at 1h following AFP, peaking between 1 and 24h post-treatment, only then to decline significantly within the following 7 days.</p></div><div><h3>Conclusion</h3><p>The human skin explant model can be used to bridge the gap between <em>in vitro</em> cell culture experiments and <em>in vivo</em> investigations to study the spatio-temporal effects of AFP on human skin. Specifically, CO<sub>2</sub>-laser AFP of skin explants resulted in an energy-dependent, short-lived up-regulation of HSP70 expression predominantly in the epidermal compartment.</p></div>","PeriodicalId":88584,"journal":{"name":"Medical laser application : international journal for laser treatment and research","volume":"25 3","pages":"Pages 173-180"},"PeriodicalIF":0.0,"publicationDate":"2010-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mla.2009.12.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54920597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Bioluminescent light diffusion in a four-layered turbid medium 生物发光光在四层浑浊介质中的扩散

Medical laser application : international journal for laser treatment and research Pub Date : 2010-08-01 DOI: 10.1016/j.mla.2010.05.003

André Liemert, Alwin Kienle

引用次数: 1

Present and alternative dosimetry concept for laser exposure limits 当前和替代剂量学概念的激光照射限值

Medical laser application : international journal for laser treatment and research Pub Date : 2010-04-01 DOI: 10.1016/j.mla.2010.02.003

Karl Schulmeister

{"title":"Present and alternative dosimetry concept for laser exposure limits","authors":"Karl Schulmeister","doi":"10.1016/j.mla.2010.02.003","DOIUrl":"10.1016/j.mla.2010.02.003","url":null,"abstract":"<div><p>In order to perform a quantitative laser safety analysis, it is necessary to compare the exposure limit (EL) for the eye or the skin with the expected exposure level in terms of irradiance or radiant exposure. The exposure level, however, is not necessarily the actual physical irradiance or radiant exposure, but is a value that is averaged over an aperture with a defined diameter. When the laser beam is smaller than the averaging aperture, the resulting “biologically effective” irradiance or radiant exposure value is much smaller than the actual value.</p><p>The background of the averaging aperture sizes that are specified is discussed together with the ELs for laser radiation. For the wavelength range where the retina is at risk (400–1400nm) the diameter of the averaging aperture is 7mm. This aperture is be used to average the irradiance that is incident at the level of the cornea. Since the EL in this wavelength range is also given as irradiance and referenced to the position of the cornea, the concept of averaging apertures is cohesive; however, it is not intuitive and it is difficult to convey in training courses, and is often the reason for miscalculation.</p><p>An alternative, more straightforward dosimetry concept is proposed, where the EL is transformed into a “power” value by multiplication by the area of the averaging aperture. This procedure results in values which are identical with the accessible emission limits for Class 1 of IEC 60825-1. For the safety analysis, this EL (for instance 1mW) is compared to the power that passes through an aperture with a diameter of 7mm. This alternative concept is mathematically equivalent to the currently defined concept. However, in contrast to the present dosimetry concept, it is intuitive because the exposure value that is compared to the EL can be understood as “power that passes through the pupil of the eye”.</p></div>","PeriodicalId":88584,"journal":{"name":"Medical laser application : international journal for laser treatment and research","volume":"25 2","pages":"Pages 111-117"},"PeriodicalIF":0.0,"publicationDate":"2010-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mla.2010.02.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54920709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Content 内容

Medical laser application : international journal for laser treatment and research Pub Date : 2010-04-01 DOI: 10.1016/S1615-1615(10)00033-5

引用次数: 0

Vorschriften und Informationsmaterial zur Unfallverhütung beim Umgang mit Laserstrahlung und IPL in Deutschland, Österreich und der Schweiz 德国、奥地利和瑞士关于预防激光辐射的规章和资料材料

Medical laser application : international journal for laser treatment and research Pub Date : 2010-04-01 DOI: 10.1016/j.mla.2010.02.004

引用次数: 0

Light-assisted therapy in dermatology: The use of intense pulsed light (IPL) 皮肤病学中的光辅助治疗:强脉冲光(IPL)的应用

Medical laser application : international journal for laser treatment and research Pub Date : 2010-04-01 DOI: 10.1016/j.mla.2010.01.001

Philipp Babilas

{"title":"Light-assisted therapy in dermatology: The use of intense pulsed light (IPL)","authors":"Philipp Babilas","doi":"10.1016/j.mla.2010.01.001","DOIUrl":"10.1016/j.mla.2010.01.001","url":null,"abstract":"<div><p>Light is electromagnetic radiation and human beings encounter it on a number of levels. The versatility of the cutaneous biological effects allows the medical utilization of light to be used as a potential diagnostic and therapeutic tool. In almost all cases the light used is restricted to certain wavelengths and defined photophysical parameters. In terms of diagnostic purposes photo testing, Wood's light diagnostics and fluorescence diagnostics are frequently used tools. The therapeutic use of incoherent light can be divided in four main groups: (1) UV phototherapy, (2) photodynamic therapy (PDT), (3) treatment with light-emitting diodes (LEDs), and (4) treatment with intense pulsed light (IPL). Intense pulsed light (IPL) devices use flashlamps and bandpass filters to emit polychromatic, incoherent, high-intensity pulsed light of a determined wavelength spectrum, fluence, and pulse duration. Similar to lasers, the basic principle of IPL devices is the selective thermal damage of the target structure. This review summarizes the practical use of IPL devices and the current literature on IPL with regard to the treatment of unwanted hair growth, vascular lesions, pigmented lesions, acne vulgaris, and as a light source for PDT.</p></div>","PeriodicalId":88584,"journal":{"name":"Medical laser application : international journal for laser treatment and research","volume":"25 2","pages":"Pages 61-69"},"PeriodicalIF":0.0,"publicationDate":"2010-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mla.2010.01.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54920611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Eye protection for short and ultra-short pulsed laser systems 短脉冲和超短脉冲激光系统的眼部保护

Medical laser application : international journal for laser treatment and research Pub Date : 2010-04-01 DOI: 10.1016/j.mla.2010.01.004

Alfred Schirmacher

引用次数: 2

Endotracheal tube fires still happen – A short overview 气管内管起火仍然会发生-简要概述

Medical laser application : international journal for laser treatment and research Pub Date : 2010-04-01 DOI: 10.1016/j.mla.2010.01.006

Wolfgang Wöllmer , Götz Schade , Gerhard Kessler

{"title":"Endotracheal tube fires still happen – A short overview","authors":"Wolfgang Wöllmer , Götz Schade , Gerhard Kessler","doi":"10.1016/j.mla.2010.01.006","DOIUrl":"10.1016/j.mla.2010.01.006","url":null,"abstract":"<div><p>Laser generated operating room fires have been reported ever since lasers have been used for therapeutic indications. This occurs because, when an ignition source such as the laser, is used in the vicinity of high oxygen concentrations, and materials, such as the endotracheal tube (ETT), can then be easily ignited. This paper shows examples of ETTs after such a fire, together with the severe injuries incurred. Even though over the years a great deal of experience has been collected in the management and handling of these patients, accidents with ETT fires still occur.</p><p>Many different materials have been tested for ETTs with regard to their incendiary characteristics, and special constructions of ETTs for use with lasers have been developed accordingly. Whereas wrapping the ETT with metal foil gives a false sense of safety, the so-called ‘laser-tubes’ exhibit an increased resistance to damage by laser radiation. However, even using these, ETT fires have still occurred due to contamination with blood or because the laser has hit the connection of the cuff with the shaft of the tube. These particular aspects have recently been investigated, and international standards are being prepared, which will hopefully promote the development of proven laser-suited ETTs in an effort to reduce the frequency of these severe accidents.</p></div>","PeriodicalId":88584,"journal":{"name":"Medical laser application : international journal for laser treatment and research","volume":"25 2","pages":"Pages 118-125"},"PeriodicalIF":0.0,"publicationDate":"2010-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mla.2010.01.006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54920685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7