Biotechnology Progress最新文献_第5页

Online monitored characterization of Phocaeicola vulgatus for organic acid production using anaerobic microtiter plate cultivations. 利用厌氧微孔板培养在线监测 Phocaeicola vulgatus 生产有机酸的特征。

IF 2.5 3区生物学

Biotechnology Progress Pub Date : 2024-12-20 DOI: 10.1002/btpr.3526

Laura Keitel, Benjamin Schick, Gino Pohen, Stanislav Yordanov, Jochen Büchs

{"title":"Online monitored characterization of Phocaeicola vulgatus for organic acid production using anaerobic microtiter plate cultivations.","authors":"Laura Keitel, Benjamin Schick, Gino Pohen, Stanislav Yordanov, Jochen Büchs","doi":"10.1002/btpr.3526","DOIUrl":"https://doi.org/10.1002/btpr.3526","url":null,"abstract":"Phocaeicola vulgatus (formerly Bacteroides vulgatus), an anaerobic gut bacterium, produces several organic acids. Research on P. vulgatus is still in its infancy. However, a detailed understanding of P. vulgatus growth and metabolism is essential for its assessment as an organic acid producer. Media variations, including different initial glucose and NH4Cl concentrations and osmolalities, are significant means to yield higher organic acid titers. Furthermore, examining different nitrogen and carbon sources is important to evaluate the potential of P. vulgatus for growth on renewable resources. Cultivations were performed in an in-house built device for anaerobic online-monitoring of fluorescence and scattered light in microtiter plates. Results revealed that the highest organic acid concentrations were reached while using galactose, glucose, or xylose as a carbon source, high osmolalities, and 0.25 g L-1 NH4Cl. In addition, the organic acid composition changed with changing carbon and nitrogen sources. P. vulgatus was successfully further characterized, thereby contributing to a faster characterization of other anaerobic strains and paving the way for anaerobic organic acid production.","PeriodicalId":8856,"journal":{"name":"Biotechnology Progress","volume":" ","pages":"e3526"},"PeriodicalIF":2.5,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-sensitivity real-time monitoring of pH and respiration activity unveils metabolic dynamics in shake flask cultures. 高灵敏度实时监测pH值和呼吸活动揭示了摇瓶培养的代谢动力学。

IF 2.5 3区生物学

Biotechnology Progress Pub Date : 2024-12-13 DOI: 10.1002/btpr.3525

Burak Sarikaya, Karsten Günster, Luca Antonia Grebe, Eva Forsten, Katharina Hürter, Jochen Büchs, Jørgen Magnus

{"title":"High-sensitivity real-time monitoring of pH and respiration activity unveils metabolic dynamics in shake flask cultures.","authors":"Burak Sarikaya, Karsten Günster, Luca Antonia Grebe, Eva Forsten, Katharina Hürter, Jochen Büchs, Jørgen Magnus","doi":"10.1002/btpr.3525","DOIUrl":"https://doi.org/10.1002/btpr.3525","url":null,"abstract":"Erlenmeyer shake flasks are widely used during the first steps of bioprocess development. Despite their broad application in academia and industry, shake flasks usually lack standardized and user-friendly online monitoring techniques. In this work, the pH and Respiratory Activity MOnitoring System (pH-RAMOS) for the non-invasive online measurement of the oxygen transfer rate (OTR), carbon dioxide transfer rate (CTR), and pH in up to eight parallel shake flasks under sterile conditions is presented. The OTR and CTR are quasi-continuously measured in the headspace of the shake flasks using dedicated oxygen and carbon dioxide sensors, enabling precise respiratory quotient (RQ) evaluation. Self-adhesive pH sensor spots are used for the high-frequent real-time pH monitoring of the culture. These prototype pH sensor spots stand out due to their simple sterilizability and subsequent one-point calibration in the cultivation medium. The long-term stability of the pH sensor spots was assessed in a 28-day long abiotic experiment. The novel pH-RAMOS was validated with different eukaryotic and prokaryotic microorganisms, such as Ogataea polymorpha, Ustilago trichophora, and Vibrio natriegens. The combination of online OTR, CTR, RQ, and pH signals allowed for identifying various metabolic phenomena, such as oxygen limitations, substrate limitations, diauxies, and the production or consumption of specific compounds, based on their degree of reduction or change of pH. The high-frequent and sensitive pH-monitoring was particularly advantageous for registering subtle and transient metabolic phenomena.","PeriodicalId":8856,"journal":{"name":"Biotechnology Progress","volume":" ","pages":"e3525"},"PeriodicalIF":2.5,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrospun gelatin/hyaluronic acid nanofibers as a platform for uric acid delivery to neural tissue. 电纺明胶/透明质酸纳米纤维作为向神经组织输送尿酸的平台。

IF 2.5 3区生物学

Biotechnology Progress Pub Date : 2024-11-10 DOI: 10.1002/btpr.3517

Reva M Street, Frank H Kung, Laura T Beringer, Daniel B Amchin, Bonnie L Firestein, Caroline L Schauer

{"title":"Electrospun gelatin/hyaluronic acid nanofibers as a platform for uric acid delivery to neural tissue.","authors":"Reva M Street, Frank H Kung, Laura T Beringer, Daniel B Amchin, Bonnie L Firestein, Caroline L Schauer","doi":"10.1002/btpr.3517","DOIUrl":"https://doi.org/10.1002/btpr.3517","url":null,"abstract":"Uric acid (UA) is an antioxidant that has been reported to be a neuroprotective compound for injuries and diseases, and specifically, diseases of the central nervous system. However, uric acid is highly insoluble in aqueous solutions, and high levels in the serum lead to gout, which limits its use in humans. Here, we develop a novel drug delivery platform that will release uric acid in a sustained manner for application to neural tissue. We demonstrate that one-step incorporation of UA into an electrospun gelatin/hyaluronic acid nanofiber mat results in controlled release of UA in culture medium. Taking a unique approach, we made solutions of 12% gelatin and 1% hyaluronic acid in a formic acid solvent and added UA for production of nanofiber mats. We then dehydrothermally crosslinked the mats and tested for release of UA into physiological cell culture medium. To test whether the mats have any detrimental effects on healthy nervous system tissue, we cultured spinal cord explants on the mats extended and assessed extensions from the explants. We observed that comparable numbers and lengths of dendrites are extended from the spinal cord tissue, regardless of the amount UA content in the mats. Our results suggest that electrospun gelatin/hyaluronic acid nanofibers can be used as a platform for sustained uric acid delivery to neural tissue without detrimental effects.","PeriodicalId":8856,"journal":{"name":"Biotechnology Progress","volume":" ","pages":"e3517"},"PeriodicalIF":2.5,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142614088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-thermal plasma decontamination of microbes: a state of the art. 微生物的非热等离子净化：最新技术。

IF 2.5 3区生物学

Biotechnology Progress Pub Date : 2024-10-27 DOI: 10.1002/btpr.3511

Yiyi Xu, Amarjeet Bassi

{"title":"Non-thermal plasma decontamination of microbes: a state of the art.","authors":"Yiyi Xu, Amarjeet Bassi","doi":"10.1002/btpr.3511","DOIUrl":"https://doi.org/10.1002/btpr.3511","url":null,"abstract":"Microbial decontamination is a critical concern in various sectors, from healthcare to food processing. Traditional decontamination methods, while effective to a degree, present limitations in terms of environmental impact, efficiency, and potential harm to the target material. This review investigates the emerging realm of non-thermal plasma (NTP) as a promising alternative for microbial decontamination, emphasizing its mechanisms, reactor designs and applications. The mechanism decomposed into physical, chemical and biological effects of plasma, are elaborated upon to provide a foundational understanding of the intrinsic principles of plasma decontamination. Except for the generation type of NTP, reactors and other parameters by which NTP achieves microbial decontamination, emphasizing the design considerations and parameters that influence its efficacy. Moreover, the latest applications of NTP in decontaminating air, water, and surfaces, supported by the latest research findings in each domain are explored. Additionally, the perspectives on the future research tendencies of NTP decontamination and disinfection are highlighted with potential avenues for exploration and innovation. Through this comprehensive review, the aim is to underscore the potential of NTP, particularly DBD plasma, as a versatile, efficient, and environmentally friendly method for microbial decontamination.","PeriodicalId":8856,"journal":{"name":"Biotechnology Progress","volume":" ","pages":"e3511"},"PeriodicalIF":2.5,"publicationDate":"2024-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanistic model of minute virus of mice elution behavior in anion exchange chromatography purification. 阴离子交换色谱纯化过程中小鼠微小病毒洗脱行为的机理模型。

IF 2.5 3区生物学

Biotechnology Progress Pub Date : 2024-10-23 DOI: 10.1002/btpr.3516

Ryunosuke Kitamura, Lena Enghauser, Riku Miyamoto, Takahiro Ichikawa, Takaki Aiso, Yumiko Masuda, Daisuke Kajihara, Hirofumi Kakihara, Koichi Nonaka

{"title":"Mechanistic model of minute virus of mice elution behavior in anion exchange chromatography purification.","authors":"Ryunosuke Kitamura, Lena Enghauser, Riku Miyamoto, Takahiro Ichikawa, Takaki Aiso, Yumiko Masuda, Daisuke Kajihara, Hirofumi Kakihara, Koichi Nonaka","doi":"10.1002/btpr.3516","DOIUrl":"https://doi.org/10.1002/btpr.3516","url":null,"abstract":"This study aimed to propose a methodology for developing a mechanistic model for viral clearance of the minute virus of mice (MVM) on flow-through anion exchange (AEX) chromatography. Protein surface analysis was applied to investigate the possibility of molecular interaction between the recombinant biotherapeutic and MVM. The protein product-free Tris buffers were spiked with MVM, and the MVM elution profile from AEX chromatography was quantitatively analyzed using quantitative polymerase chain reaction (qPCR) for pooled fractions. GoSilico™ Chromatography Modeling Software was applied to develop the mechanistic models for MVM species. For evaluating the visual fit of the developed model, the R2 of intact MVM virions and uncoated capsids between the simulated and measured amount in each fraction are 0.880 and 0.948, respectively. Response surface plots of logarithmic reduction values (LRV) against pH and conductivity in loaded sample were generated to show the range for suitable loaded sample conditions for achieving a good LRV. To evaluate the applicability of the developed MVM elution model to a recombinant biotherapeutic, two demonstrations of AEX chromatography purification were performed with a loaded sample of a model monoclonal antibody. The peaks of the MVM species in the elution step of both runs were accurately simulated by the developed model. In addition, to assess the possibility of molecular interaction between the virus and the target protein significantly affecting the MVM elution behavior, the antibody's surface was evaluated in terms of hydrophobicity/hydrophilicity using surface analysis.","PeriodicalId":8856,"journal":{"name":"Biotechnology Progress","volume":" ","pages":"e3516"},"PeriodicalIF":2.5,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparing in silico flowsheet optimization strategies in biopharmaceutical downstream processes. 比较生物制药下游工艺中的硅学流程优化策略。

IF 2.5 3区生物学

Biotechnology Progress Pub Date : 2024-10-18 DOI: 10.1002/btpr.3514

Daphne Keulen, Myrto Apostolidi, Geoffroy Geldhof, Olivier Le Bussy, Martin Pabst, Marcel Ottens

{"title":"Comparing in silico flowsheet optimization strategies in biopharmaceutical downstream processes.","authors":"Daphne Keulen, Myrto Apostolidi, Geoffroy Geldhof, Olivier Le Bussy, Martin Pabst, Marcel Ottens","doi":"10.1002/btpr.3514","DOIUrl":"https://doi.org/10.1002/btpr.3514","url":null,"abstract":"The challenging task of designing biopharmaceutical downstream processes is initially to select the type of unit operations, followed by optimizing their operating conditions. For complex flowsheet optimizations, the strategy becomes crucial in terms of duration and outcome. In this study, we compared three optimization strategies, namely, simultaneous, top-to-bottom, and superstructure decomposition. Moreover, all strategies were evaluated by either using chromatographic Mechanistic Models (MMs) or Artificial Neural Networks (ANNs). An overall evaluation of 39 flowsheets was performed, including a buffer-exchange step between the chromatography operations. All strategies identified orthogonal structures to be optimal, and the weighted overall performance values were generally consistent between the MMs and ANNs. In terms of time-efficiency, the decomposition method with MMs stands out when utilizing multiple cores on a multiprocessing system for simulations. This study analyses the influence of different optimization strategies on flowsheet optimization and advices on suitable strategies and modeling techniques for specific scenarios.","PeriodicalId":8856,"journal":{"name":"Biotechnology Progress","volume":" ","pages":"e3514"},"PeriodicalIF":2.5,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

General strategies for IgG-like bispecific antibody purification. IgG 样双特异性抗体纯化的一般策略。

IF 2.5 3区生物学

Biotechnology Progress Pub Date : 2024-10-15 DOI: 10.1002/btpr.3515

Yifeng Li

{"title":"General strategies for IgG-like bispecific antibody purification.","authors":"Yifeng Li","doi":"10.1002/btpr.3515","DOIUrl":"https://doi.org/10.1002/btpr.3515","url":null,"abstract":"Bispecific antibodies (bsAbs) can simultaneously bind two different antigens or epitopes. Their dual-targeting capability enables novel mechanisms of action, gaining therapeutic advantages over conventional monospecific mAbs. In recent years, the number of bsAbs grows rapidly and bsAbs under development are available in diverse formats. In particular, Fc-containing IgG-like bsAbs, which represent the major group, can be constructed in asymmetric or symmetric format. For asymmetric ones, whose assembly requires multiple distinct chains, although numerous strategies have been developed to promote desired chain pairing, product-related variants such as free chains, half molecules and mispaired species are usually present at various levels. For symmetric ones, increased level of aggregates and truncating variants is often associated with their production. In general, bsAbs pose greater challenges to the downstream team than regular mAbs. In the past few years, our team successfully developed the downstream process for over 70 bsAbs in greater than 30 different formats and accumulated substantial experience. This review introduces general strategies that we have used while purifying these challenging molecules.","PeriodicalId":8856,"journal":{"name":"Biotechnology Progress","volume":" ","pages":"e3515"},"PeriodicalIF":2.5,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the biocatalysis of psilocybin and other tryptamines: Enzymatic pathways, synthetic strategies, and industrial implications. 探索迷幻药和其他色胺的生物催化：酶途径、合成策略和工业影响。

IF 2.5 3区生物学

Biotechnology Progress Pub Date : 2024-10-04 DOI: 10.1002/btpr.3513

Lucas Henrique Junges, Marcelo Müller-Santos

引用次数: 0

From protein structure to an optimized chromatographic capture step using multiscale modeling 利用多尺度建模从蛋白质结构到优化色谱捕获步骤。

IF 2.5 3区生物学

Biotechnology Progress Pub Date : 2024-09-29 DOI: 10.1002/btpr.3505

Daphne Keulen, Tim Neijenhuis, Adamantia Lazopoulou, Roxana Disela, Geoffroy Geldhof, Olivier Le Bussy, Marieke E. Klijn, Marcel Ottens

{"title":"From protein structure to an optimized chromatographic capture step using multiscale modeling","authors":"Daphne Keulen, Tim Neijenhuis, Adamantia Lazopoulou, Roxana Disela, Geoffroy Geldhof, Olivier Le Bussy, Marieke E. Klijn, Marcel Ottens","doi":"10.1002/btpr.3505","DOIUrl":"10.1002/btpr.3505","url":null,"abstract":"Optimizing a biopharmaceutical chromatographic purification process is currently the greatest challenge during process development. A lack of process understanding calls for extensive experimental efforts in pursuit of an optimal process. In silico techniques, such as mechanistic or data driven modeling, enhance the understanding, allowing more cost-effective and time efficient process optimization. This work presents a modeling strategy integrating quantitative structure property relationship (QSPR) models and chromatographic mechanistic models (MM) to optimize a cation exchange (CEX) capture step, limiting experiments. In QSPR, structural characteristics obtained from the protein structure are used to describe physicochemical behavior. This QSPR information can be applied in MM to predict the chromatogram and optimize the entire process. To validate this approach, retention profiles of six proteins were determined experimentally from mixtures, at different pH (3.5, 4.3, 5.0, and 7.0). Four proteins at different pH's were used to train QSPR models predicting the retention volumes and characteristic charge, subsequently the equilibrium constant was determined. For an unseen protein knowing only the protein structure, the retention peak difference between the modeled and experimental peaks was 0.2% relative to the gradient length (60 column volume). Next, the CEX capture step was optimized, demonstrating a consistent result in both the experimental and QSPR-based methods. The impact of model parameter confidence on the final optimization revealed two viable process conditions, one of which is similar to the optimization achieved using experimentally obtained parameters. The multiscale modeling approach reduces the required experimental effort by identification of initial process conditions, which can be optimized.","PeriodicalId":8856,"journal":{"name":"Biotechnology Progress","volume":"41 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/btpr.3505","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CHO stable pool fed-batch process development of SARS-CoV-2 spike protein production: Impact of aeration conditions and feeding strategies 生产 SARS-CoV-2 穗状病毒蛋白的 CHO 稳定池喂料批次工艺开发：通气条件和喂料策略的影响。

IF 2.5 3区生物学

Biotechnology Progress Pub Date : 2024-09-27 DOI: 10.1002/btpr.3507

Sebastian-Juan Reyes, Phuong Lan Pham, Yves Durocher, Olivier Henry

{"title":"CHO stable pool fed-batch process development of SARS-CoV-2 spike protein production: Impact of aeration conditions and feeding strategies","authors":"Sebastian-Juan Reyes, Phuong Lan Pham, Yves Durocher, Olivier Henry","doi":"10.1002/btpr.3507","DOIUrl":"10.1002/btpr.3507","url":null,"abstract":"Technology scale-up and transfer are a fundamental and critical part of process development in biomanufacturing. Important bioreactor hydrodynamic characteristics such as working volume, overhead gas flow rate, volumetric power input (P/V), impeller type, agitation regimen, sparging aeration strategy, sparger type, and kLa must be selected based on key performance indicators (KPI) to ensure a smooth and seamless process scale-up and transfer. Finding suitable operational setpoints and developing an efficient feeding regimen to ensure process efficacy and consistency are instrumental. In this investigation, process development of a cumate inducible Chinese hamster ovary (CHO) stable pool expressing trimeric SARS-CoV-2 spike protein in 1.8 L benchtop stirred-tank bioreactors is detailed. Various dissolved oxygen levels and aeration air caps were studied to determine their impact on cell growth and metabolism, culture longevity, and endpoint product titers. Once hydrodynamic conditions were tuned to an optimal zone, various feeding strategies were explored to increase culture performance. Dynamic feedings such as feeding based on current culture volume, viable cell density (VCD), oxygen uptake rate (OUR), and bio-capacitance signals were tested and compared to standard bolus addition. Increases in integral of viable cell concentration (IVCC) (1.25-fold) and protein yield (2.52-fold), as well as greater culture longevity (extension of 5 days) were observed in dynamic feeding strategies when compared to periodic bolus feeding. Our study emphasizes the benefits of designing feeding strategies around metabolically relevant signals such as OUR and bio-capacitance signals.","PeriodicalId":8856,"journal":{"name":"Biotechnology Progress","volume":"41 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/btpr.3507","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0