Biologia futura最新文献_第5页

Molecular and functional diversity of plant associated microbes: biotechnological potential, genomics and commercialization for climate resilient agriculture. 植物相关微生物的分子和功能多样性：生物技术潜力、基因组学和气候适应型农业的商业化。

IF 2.1 4区生物学

Biologia futura Pub Date : 2026-06-01 Epub Date: 2026-02-24 DOI: 10.1007/s42977-026-00308-3

Divjot Kour, Tanvir Kaur, Sofia Sharief Khan, Damini Maithani, Babita Sharma, Hemant Dasila, Priyanka Mahajan, Tishu Devi, Arshdeep Singh

{"title":"Molecular and functional diversity of plant associated microbes: biotechnological potential, genomics and commercialization for climate resilient agriculture.","authors":"Divjot Kour, Tanvir Kaur, Sofia Sharief Khan, Damini Maithani, Babita Sharma, Hemant Dasila, Priyanka Mahajan, Tishu Devi, Arshdeep Singh","doi":"10.1007/s42977-026-00308-3","DOIUrl":"10.1007/s42977-026-00308-3","url":null,"abstract":"Plants harbor a huge diversity of the microbes in their rhizosphere, internal tissues and aerial parts. The beneficial interactions between the plants and their microbes occur by direct and indirect mechanisms which impact growth and overall health of the plants. These mechanisms include production of phytohormones, solubilization and availability of nutrients for plant uptake, production of hydrolytic enzymes and other compounds for protecting them against the pathogens. These microbes offer benefits of combating the abiotic stresses to which plants are exposed. Looking at the importance of beneficial plant-microbe interactions and need for economical solutions for meeting global healthy food demands, the beneficial microbes could be developed as bio-inoculants for sustainable agricultural biotechnology under changing climate. Depending on their mode of action, inoculants could be used as biofertilizers, biopesticides, and stress alleviators. There is a strong growing market for bio-inoculants so that the application of the chemical fertilizers could be reduced. The present review highlights the functional diversity of the plant associated microbes in major crops, their mechanisms of action and role as biofertilizers, biocontrol agents, in stress mitigation and bioremediation. Overall, exploration of the beneficial plant associated microbes and their use as bio-inoculants holds a potential for sustainable growth.","PeriodicalId":8853,"journal":{"name":"Biologia futura","volume":" ","pages":"277-320"},"PeriodicalIF":2.1,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147282188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined use of proline and microbes on yield attributes, photosynthetic efficiency, water relations, nutrient acquisition and antioxidative defense mechanisms of fennel plants under drought conditions. 干旱条件下脯氨酸和微生物对茴香产量属性、光合效率、水分关系、养分获取和抗氧化防御机制的影响

IF 1.5 4区生物学

Biologia futura Pub Date : 2026-03-01 Epub Date: 2025-11-28 DOI: 10.1007/s42977-025-00293-z

Tabish Ali Virk, Muhammad Zulqurnain Haider, Qasim Ali

{"title":"Combined use of proline and microbes on yield attributes, photosynthetic efficiency, water relations, nutrient acquisition and antioxidative defense mechanisms of fennel plants under drought conditions.","authors":"Tabish Ali Virk, Muhammad Zulqurnain Haider, Qasim Ali","doi":"10.1007/s42977-025-00293-z","DOIUrl":"10.1007/s42977-025-00293-z","url":null,"abstract":"The aim of the study was the induction of water stress tolerance in the fennel (Foeniculum vulgare Mill.) by the combined proline (Pro) and bacterial strain BEB1 (BEB1) application as foliar spray. Arrangement of experiment was done in field as split-split plot in open natural environmental conditions. Deficit water supply significantly adversely affected the growth and yield of fennel plants. Foliar-applied different treatments significantly reduced the water stress adverse effects on the performance of fennel plants regarding growth and yield that found linked to improvement in water status of plants and better net photosynthetic activity of fennel plants in comparison to non-treated plants. Combined application of Pro and BEB1 better maintained the seed yield and growth of fennel plants found associated with reduced lipid peroxidation, improvement in antioxidative defence mechanism, maintenance of plant water relations through better osmotic adjustment by increased accumulation of different metabolites. The maintenance of better yield and biomass production of water-stressed fennel plants also found positively associated with increased uptake as well as translocation of mineral nutrients. The maximum increase (30, 35 and 36, 34%) in shoot and root fresh and dry biomasses respectively and a decrease (24.13, 23.01 and 25.75%) in LRMP, MDA and H2O2 respectively, was found in plants sprayed with 1%Pro + BEB1. An increase of 41.43% in seed yield was recorded in fennel plants sprayed with 1%Pro + BEB1. Combined foliar spray of Pro and BEB1 was found better to increase tolerance against water stress for better yield by improving physio-biochemical mechanisms than their individual applications.","PeriodicalId":8853,"journal":{"name":"Biologia futura","volume":" ","pages":"113-138"},"PeriodicalIF":1.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145628411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nature-based solutions: the potential of bryophytes for snail repellency in lettuce crop production. 基于自然的解决方案：苔藓植物在生菜作物生产中驱螺的潜力。

IF 1.5 4区生物学

Biologia futura Pub Date : 2026-03-01 Epub Date: 2025-11-27 DOI: 10.1007/s42977-025-00297-9

Nikolina A Matić, Marija V Vesović, Djordje P Božović, Milorad M Vujičić, Michal Goga, Aneta D Sabovljević, Marko S Sabovljević

{"title":"Nature-based solutions: the potential of bryophytes for snail repellency in lettuce crop production.","authors":"Nikolina A Matić, Marija V Vesović, Djordje P Božović, Milorad M Vujičić, Michal Goga, Aneta D Sabovljević, Marko S Sabovljević","doi":"10.1007/s42977-025-00297-9","DOIUrl":"10.1007/s42977-025-00297-9","url":null,"abstract":"Damage in lettuce production caused by burgundy snails has led to an increased use of molluscicidal chemicals, which persist in both the lettuce and the substrate, and accumulate over time with undesirable outcomes in terms of mollusk pest control. Moreover, these chemicals also harm natural snail populations. In this study, the effects of selected bryophyte species extracts and their application concentrations were tested as natural snail repellents. Twelve bryophyte species, eight mosses, and four liverworts were randomly selected, and their ethanolic extracts were applied as burgundy snail repellents in lettuce. Significant antifeeding effects were observed for the liverworts Bazzania trilobata, Plagiochila asplenioides, and Porella platyphylla, as well as for the mosses Fontinalis antipyretica, Mnium stellare, and Neckera crispa. Among these, B. trilobata exhibited the strongest antifeeding effect without negatively impacting lettuce growth and development, as assessed through seed germination, hypocotyl length, and seedling total chlorophyll content. All bryophyte species with significant antifeeding i.e. repellent potential shared similar chemical profiles, characterized by high phenolic and triterpenoid contents and strong antioxidant capacity. The results obtained offer a nature-based solution in lettuce crop protection as well as an eco-friendly alternative in food production.","PeriodicalId":8853,"journal":{"name":"Biologia futura","volume":" ","pages":"175-188"},"PeriodicalIF":1.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145628403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatial analysis and niche overlap of two lapwing (Genus: Vanellus) species in the confluence of two biodiversity hotspots in South Asia. 南亚两个生物多样性热点汇合处两种田鸡种的空间分析及生态位重叠

IF 2.1 4区生物学

Biologia futura Pub Date : 2026-03-01 Epub Date: 2025-11-20 DOI: 10.1007/s42977-025-00296-w

Langtuk Terang, Vivek Chetry, Aditya Shankar Kataki, Anubhav Bhuyan, Kuladip Sarma

{"title":"Spatial analysis and niche overlap of two lapwing (Genus: Vanellus) species in the confluence of two biodiversity hotspots in South Asia.","authors":"Langtuk Terang, Vivek Chetry, Aditya Shankar Kataki, Anubhav Bhuyan, Kuladip Sarma","doi":"10.1007/s42977-025-00296-w","DOIUrl":"10.1007/s42977-025-00296-w","url":null,"abstract":"Environmental factors play a critical role in shaping species distribution and resource utilization patterns. However, a gap of knowledge exists in understanding the niche dynamics between the two species; the ‘Least Concern’- Vanellus indicus (Red-wattled Lapwing) and the ‘Near Threatened’-Vanellus duvaucelii (River Lapwing), a difference that could prove critical for long-term conservation efforts. Thus, the present study aims to understand whether environmental variables influence their spatial patterns and examine the potential impact of niche overlap. Using primary and secondary species occurrence data, combined with environmental datasets, we modelled suitable habitats using an ensemble approach for each species with the SSDM package in R. Ensemble models performed well, yielding good predictive performance for both Vanellus indicus (AUC = 0.89) and Vanellus duvaucelii (AUC = 0.88). Predicted habitat areas for V. indicus were 28,818.35 km2 (high suitability), 23,101.29 km2 (moderate suitability), and 29,815.65 km2 (low suitability). For V. duvaucelii, these values were 12,429.95 km2, 26,452.24 km2, and 53,775.59 km2, respectively. Niche overlap, measured with Schoener’s D, was 0.55, indicating moderate similarity, with 0.07 and 0.04 representing unique niches for V. duvaucelii and V. indicus, respectively, and 0.96 of the being niches shared. These results underscore the ecological implications of niche sharing between overlapping species and highlight potential risks for V. duvaucelii, as overlapping habitats and limited niche exclusivity could increase vulnerability under rapidly changing environmental conditions. These findings further highlight the ecological consequences of niche sharing between the species and point out possible risks for V. duvaucelii, and thus poor niche separation may increase vulnerability under rapid environmental change.","PeriodicalId":8853,"journal":{"name":"Biologia futura","volume":" ","pages":"165-174"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145562568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Central administration of asprosin reduces baseline brain activity but does not attenuate 4-AP-induced epileptiform discharges: an in vivo electrophysiological study. 一项体内电生理研究表明，阿斯普罗辛的中央管理降低基线脑活动，但不减弱4- ap诱导的癫痫样放电。

IF 1.5 4区生物学

Biologia futura Pub Date : 2026-03-01 Epub Date: 2025-11-12 DOI: 10.1007/s42977-025-00295-x

Ömer Faruk Kalkan, Abdulhamit Yildirim, Osman Aktaş, Zafer Şahin, Selcen Aydin Abidin, Ali Faruk Özyaşar, Abdullah Kaan Kurt, Samet Soner Küçükosman, İbrahim Uzun, İsmail Abidin

{"title":"Central administration of asprosin reduces baseline brain activity but does not attenuate 4-AP-induced epileptiform discharges: an in vivo electrophysiological study.","authors":"Ömer Faruk Kalkan, Abdulhamit Yildirim, Osman Aktaş, Zafer Şahin, Selcen Aydin Abidin, Ali Faruk Özyaşar, Abdullah Kaan Kurt, Samet Soner Küçükosman, İbrahim Uzun, İsmail Abidin","doi":"10.1007/s42977-025-00295-x","DOIUrl":"10.1007/s42977-025-00295-x","url":null,"abstract":"In the current study, we investigated the effects of central asprosin administration on baseline brain activity and 4-aminopyridine (4-AP) induced epileptiform activity. Additionally, we examined the effects of asprosin on electrocorticography (ECoG) band powers. Forty-two male Wistar rats were divided into six groups as follows: sham, 4-AP (2.5 mg/kg i.p.), asprosin 20 nmol (i.c.v.), asprosin 50 nmol (i.c.v.), asprosin 50 nmol (i.c.v.) posttreatment, and asprosin 50 nmol (i.c.v.) pre-treatment. Recordings lasting 60-70 min were conducted for all groups under ketamine/xylazine (90/10 mg/kg) anesthesia. In the posttreatment group, asprosin was injected 20 min after the induction of epileptiform activity. In the pre-treatment group, asprosin was injected after baseline recordings, and following a 20-min pre-treatment period, 4-AP was administered. 4-AP alone induced epileptiform activity in all animals, peaking at approximately the 30th minute. Asprosin significantly reduced ECoG power at doses of 20 nmol and 50 nmol. Furthermore, both doses of asprosin reduced alpha, beta, delta, theta, and gamma band activity in ECoG recordings at various time points. However, asprosin pre- and posttreatment had no significant effect on 4-AP-induced epileptiform activity. These findings suggest that asprosin modulates cortical excitability under physiological conditions but is ineffective in attenuating induced epileptiform activity.","PeriodicalId":8853,"journal":{"name":"Biologia futura","volume":" ","pages":"155-164"},"PeriodicalIF":1.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145494355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PI3K/PKB signaling network as a host target to enhance bacterial ingestion and clearance. PI3K/PKB信号网络作为宿主靶点增强细菌摄入和清除。

IF 1.5 4区生物学

Biologia futura Pub Date : 2026-03-01 Epub Date: 2025-11-26 DOI: 10.1007/s42977-025-00298-8

Lou W Kim, Victor Castillo, Alejandro Barbieri

{"title":"PI3K/PKB signaling network as a host target to enhance bacterial ingestion and clearance.","authors":"Lou W Kim, Victor Castillo, Alejandro Barbieri","doi":"10.1007/s42977-025-00298-8","DOIUrl":"10.1007/s42977-025-00298-8","url":null,"abstract":"Antibiotic resistance, driven by the misuse of antibiotics and the slow pace of new drug development, has led to a global rise in multidrug-resistant bacteria (MDRB), posing a major public health challenge. Host-directed therapies that enhance innate immune responses offer promising alternatives to traditional antibiotics. This review focuses on the central role of the PI3K/PKB (Akt) signaling pathway in phagocytosis and bacterial inactivation across both primitive phagocytes, such as Dictyostelium discoideum, and mammalian immune cells. In Dictyostelium, PI3K/PKB signaling coordinates the maturation of phagosomes and the fusion of phagolysosomes, processes essential for bacterial killing. Similarly, in immune cells, this pathway regulates cytoskeletal remodeling, vesicle trafficking, and the degradation of microbes. Specific pathogens, including Mycobacterium tuberculosis and Salmonella, subvert PI3K/PKB to evade immune responses, highlighting the pathway's dual role in host defense and pathogen survival. Targeting PI3K/PKB signaling or its inhibitory regulators may enhance phagocytic efficiency and restore immune function. Thus, PI3K/PKB represents a critical module in innate immunity and a compelling target for next-generation antimicrobial strategies.","PeriodicalId":8853,"journal":{"name":"Biologia futura","volume":" ","pages":"29-37"},"PeriodicalIF":1.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145602027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photobiomodulation of tissue regeneration: low-power light therapy accelerates wound healing in earthworms. 组织再生的光生物调节：低功率光疗加速蚯蚓伤口愈合。

IF 2.1 4区生物学

Biologia futura Pub Date : 2026-03-01 Epub Date: 2025-12-11 DOI: 10.1007/s42977-025-00300-3

Saravanakumar Venkatachalam, Johnson Retnaraj Samuel Selvan Christyraj, R Kamaraj Kennedy, Jackson Durairaj Selvan Christyraj, Beryl Vedha Yesudhason

{"title":"Photobiomodulation of tissue regeneration: low-power light therapy accelerates wound healing in earthworms.","authors":"Saravanakumar Venkatachalam, Johnson Retnaraj Samuel Selvan Christyraj, R Kamaraj Kennedy, Jackson Durairaj Selvan Christyraj, Beryl Vedha Yesudhason","doi":"10.1007/s42977-025-00300-3","DOIUrl":"10.1007/s42977-025-00300-3","url":null,"abstract":"Low-power light therapy also referred to as Photobiomodulation (PBM) has emerged as a promising approach for enhancing tissue repair, yet its molecular mechanisms remain underexplored. This study investigates the effects of green, red, yellow, and white light (2-hour, 6-hour, and 24-hour exposures) on regeneration kinetics and molecular pathways in an earthworm (Eudrilus eugeniae) amputation model. Quantitative analysis revealed that 2-hour red (650 nm) and white (400–700 nm) light exposure significantly accelerated regeneration growth rates compared to other wavelengths and durations. Gene expression profiling through real-time qPCR and western blotting showed distinct pathway activation patterns: TCTP (Translationally Controlled Tumor Protein) and arrestin expression increased 2.3-fold and 1.8-fold respectively in green (525 nm) and red light groups; WNT3a signaling pathway components were upregulated 2.1-fold in white and yellow (590 nm) exposure cohorts. Notably, prolonged 24-hour exposure regimes demonstrated inhibitory effects across all wavelengths, with H2AX DNA repair markers showing elevated activity. These findings establish that brief photostimulation (particularly 2-hour red/white light) optimally activates pro-regenerative pathways through TCTP-mediated cell proliferation and WNT3a-dependent morphogenetic signaling. These results suggest wavelength-specific therapeutic windows for photobiomodulation, providing a mechanistic foundation for developing targeted light-based wound healing strategies in regenerative medicine.","PeriodicalId":8853,"journal":{"name":"Biologia futura","volume":" ","pages":"189-202"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145720927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic and genomic resources of Vigna species for their utility in advanced breeding. 葡萄属植物遗传和基因组资源及其在高级育种中的应用。

IF 2.1 4区生物学

Biologia futura Pub Date : 2026-03-01 Epub Date: 2026-02-02 DOI: 10.1007/s42977-025-00299-7

Kuldeep Kumar, Sudhir Kumar Jha, B Manu, Adarsh Kumar, Vaibhav Kumar, Khela Ram Soren, Meenal Rathore, Sandhya Sharma, Kishor Gaikwad

引用次数: 0

The future of food: seaweed farming, biotechnology, and global sustainability. 食品的未来：海藻养殖、生物技术和全球可持续性。

IF 1.5 4区生物学

Biologia futura Pub Date : 2026-03-01 Epub Date: 2025-12-29 DOI: 10.1007/s42977-025-00301-2

Poulomi Sen, Sahely Kanthal, Jayita Hore, Soham Hazra, Ria Mukhopadhyay, Ranjit Singh Gujjar, Rajeev Kumar, Avishek Chatterjee

{"title":"The future of food: seaweed farming, biotechnology, and global sustainability.","authors":"Poulomi Sen, Sahely Kanthal, Jayita Hore, Soham Hazra, Ria Mukhopadhyay, Ranjit Singh Gujjar, Rajeev Kumar, Avishek Chatterjee","doi":"10.1007/s42977-025-00301-2","DOIUrl":"10.1007/s42977-025-00301-2","url":null,"abstract":"Seaweeds, diverse marine macroalgae, are vital for ecosystems, offering significant environmental, nutritional, and economic advantages. Categorized as green, red, or brown based on pigments, they are widely used in food, medicine, and cosmetics. China leads in cultivating approximately 18 species, and innovative farming methods, like integrated multi-trophic aquaculture and advanced offshore systems, are rapidly expanding global seaweed production. This expansion considerably boosts sustainable agriculture, bioenergy, and food security. While seaweeds deliver high-value proteins, vitamins, dietary fibers, and bioactive compounds, and enhance crucial ecosystem services, their sustainable cultivation faces challenges from environmental stressors like ocean warming and pollution. Addressing these requires adaptive strategies, such as selecting resilient species and implementing effective monitoring and management. Biotechnological advancements, including tissue culture, mutation and selective breeding, and genetic engineering techniques like promoter engineering and CRISPR-based gene editing, offer promising avenues to improve yield, stress tolerance, and biochemical profiles. This comprehensive review uniquely integrates seaweed farming practices, nutritional benefits, environmental impacts, and biotechnological innovations with technological applications such as remote sensing, computational fluid dynamics, and numerical modeling for environmental monitoring and agricultural management. By synthesizing these elements, this review promotes sustainable seaweed utilization to address global challenges in food, health, and climate change.","PeriodicalId":8853,"journal":{"name":"Biologia futura","volume":" ","pages":"59-83"},"PeriodicalIF":1.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145848673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An optimized in situ hybridization protocol to study early developmental patterns and signaling pathways in paradise fish (Macropodus opercularis). 研究天堂鱼早期发育模式和信号通路的原位杂交优化方案。

IF 1.5 4区生物学

Biologia futura Pub Date : 2026-03-01 Epub Date: 2025-10-19 DOI: 10.1007/s42977-025-00290-2

Nóra Szabó, Erika Fodor, Kata Szabó, Virág Tarcsa, Zsolt Gyulai, Soma Jakab, Erika Gelei, Dávid Czimer, Ádám Miklósi, Máté Varga

{"title":"An optimized in situ hybridization protocol to study early developmental patterns and signaling pathways in paradise fish (Macropodus opercularis).","authors":"Nóra Szabó, Erika Fodor, Kata Szabó, Virág Tarcsa, Zsolt Gyulai, Soma Jakab, Erika Gelei, Dávid Czimer, Ádám Miklósi, Máté Varga","doi":"10.1007/s42977-025-00290-2","DOIUrl":"10.1007/s42977-025-00290-2","url":null,"abstract":"The Chinese paradise fish (Macropodus opercularis) is an obligate air-breathing species native to the hypoxic freshwater environments of Southeast Asia. While its ethological aspects have been extensively investigated since the 1970 s, the molecular biology of this species remains largely unexplored. Nevertheless, it offers significant potential for research in fields such as behavioral genetics, molecular evolution, and developmental biology. This study aims to optimize the in situ hybridization protocol, a widely used technique in developmental biology proven effective in zebrafish, and adapt it to paradise fish. We applied our optimized protocol to compare the expression of several conserved developmental genes, including chordin (chd), goosecoid (gsc), myogenic differentiation 1 (myod1), T box transcription factor Ta (tbxta), paired box 2a (pax2a), and retinal homebox gene 3 (rx3), in zebrafish and paradise fish embryos. Furthermore, we examined the role of key conserved signaling pathways during early development in both species using small molecular agonists and antagonists. Through these molecular analyses, we can gain deeper insights into the evolutionary conservation of early developmental programs.","PeriodicalId":8853,"journal":{"name":"Biologia futura","volume":" ","pages":"85-99"},"PeriodicalIF":1.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145318177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0