北京大学学报（医学版）最新文献

{"title":"[Research progress in diagnosis and treatment of salivary gland tumors].","authors":"Guangyan Yu, Xin Peng, Min Gao, Peng Ye, Na Ge, Mengqi Jia, Bingyu Li, Zunan Tang, Leihao Hu, Wenbo Zhang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Salivary gland tumor is one of the most common tumors in oral and maxillofacial regions. The diagnosis and treatment of salivary gland tumors had been a clinical characteristic project in Peking University School and Hospital of Stomatology since long time ago. Here we introduced the research progress in diagnosis and treatment of salivary gland tumors during the past 10 years. Among 7 190 cases of salivary gland tumors treated in this institution, 4 654 cases (64.7%) were benign, and 2 536 (35.3%) were malignant, with benign ∶ malignant ratio of 1.84 ∶ 1. Parotid was the most common location, followed by minor salivary gland and submandibular gland, while sublingular gland tumor was seldom seen. The proportion of minor salivary gland tumor was relatively high. Among 1 874 cases with primary malignant tumors, the cases with T3 and stage Ⅲ accounted for only 9.6% and 10.3%, respectively, which indicated that there was shortcoming in the T classification and clinical stage formulated by Union for International Cancer Control (UICC), and further revision was required. The 5, 10, and 15 year survival rates of 1 637 cases with postoperative follow-up were 93.1%, 87.2% and 79.3%, respectively, which were much higher than those we reported 30 years ago. The improvement of treatment results was related to more widely used combined treatment with surgery and postoperative radiotherapy, and the increase in patients with early stage. Adenoid cystic carcinoma was the malignant tumor with high rate of distant metastasis. The 5 and 10 year survival rates of the patients with pulmonary metastasis were 76.2% and 51.8%, respectively, which indicated that the pulmonary metastatic carcinomas developed slowly. Recurrent rate of carcinoma ex pleomorphic adenoma was 46.7% after single treatment of sur-gery, while it decreased to 27.5% after combined theraphy with surgery and radiotherapy, indicating that postoperative radiotheraphy could reduce the recurrent rate effectively. The normal myoepithelial cells had the inhibiting role in the invasion and metastasis of carcinoma ex pleomorphic adenoma. The evaluation of integrity of myoepithelial cells surrounding the tumor mass is helpful to understand the invasiveness of the tumors. The new surgical modalities such as extracapsular resection and partial sialoadenectomy were used in treatment of benign tumors of parotid gland and submandibular gland with advantages of decreased tissue damage and preservation of glandular function. Application of digital surgical techniques such as mixed reality combined with surgical navigation and real-time three-dimensional holograms in the surgical treatment of parotid gland tumors showed the benifits of more safety and precision, and less tissue da-mage.</p>","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"57 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11759788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Development and validation of a clinical automatic diagnosis system based on diagnostic criteria for temporomandibular disorders].","authors":"Yuanyuan Fang, Fan Xu, Jie Lei, Hao Zhang, Wenyu Zhang, Yu Sun, Hongxin Wu, Kaiyuan Fu, Weiyu Mao","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To develop a clinical automated diagnostic system for temporomandibular disorders (TMD) based on the diagnostic criteria for TMD (DC/TMD) to assist dentists in making rapid and accurate clinical diagnosis of TMD.</p><p><strong>Methods: </strong>Clinical and imaging data of 354 patients, who visited the Center for TMD & Orofacial Pain at Peking University Hospital of Stomatology from September 2023 to January 2024, were retrospectively collected. The study developed a clinical automated diagnostic system for TMD using the DC/TMD, built on the. NET Framework platform with branching statements as its internal structure. Further validation of the system on consistency and diagnostic efficacy compared with DC/TMD were also explored. Diagnostic efficacy of the TMD clinical automated diagnostic system for degenerative joint diseases, disc displacement with reduction, disc displacements without reduction with limited mouth opening and disc displacement without reduction without limited mouth opening was evaluated and compared with a specialist in the field of TMD. Accuracy, precision, specificity and the Kappa value were assessed between the TMD clinical automated diagnostic system and the specialist.</p><p><strong>Results: </strong>Diagnoses for various TMD subtypes, including pain-related TMD (arthralgia, myalgia, headache attributed to TMD) and intra-articular TMD (disc displacement with reduction, disc displacement with reduction with intermittent locking, disc displacement without reduction with limited opening, disc displacement without reduction without limited opening, degenerative joint disease and subluxation), using the TMD clinical automated diagnostic system were completely identical to those obtained by the TMD specialist based on DC/TMD. Both the system and the expert showed low sensitivity for diagnosing degenerative joint disease (0.24 and 0.37, respectively), but high specificity (0.96). Both methods achieved high accuracy (> 0.9) for diagnosing disc displacements with reduction and disc displacements without reduction with limited mouth opening. The sensitivity for diagnosing disc displacement without reduction without limited mouth opening was only 0.59 using the automated system, lower than the expert (0.87), while both had high specificity (0.92). The Kappa values for most TMD subtypes were close to 1, except the disc displacement without reduction without limited mouth opening, which had a Kappa value of 0.68.</p><p><strong>Conclusion: </strong>This study developed and validated a reliable clinical automated diagnostic system for TMD based on DC/TMD. The system is designed to facilitate the rapid and accurate diagnosis and classification of TMD, and is expected to be an important tool in clinical scenarios.</p>","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"57 1","pages":"192-201"},"PeriodicalIF":0.0,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11759806/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Evaluation of the accuracy of three-dimensional data acquisition from liquid- interference surfaces assisted by a scanner head with a compressed airflow system].","authors":"Xinkai Xu, Jianjiang Zhao, Sukun Tian, Zhongning Liu, Xiaoyi Zhao, Xiaobo Zhao, Tengfei Jiang, Xiaojun Chen, Chao Ma, Yuchun Sun","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To quantitatively evaluate the accuracy of data obtained from liquid-interference surfaces using an intraoral 3D scanner (IOS) integrated with a compressed airflow system, so as to provide clinical proof of accuracy for the application of the compressed airflow system-based scanning head in improving data quality on liquid-interference surfaces.</p><p><strong>Methods: </strong>The study selected a standard model as the scanning object, adhering to the \"YY/T 1818-2022 Dental Science Intraoral Digital Impression Scanner\" guidelines, a standard that defined parameters for intraoral scanning. To establish a baseline for accuracy, the ATOS Q 12M scanner, known for its high precision, was used to generate true reference values. These true values served as the benchmark for evaluating the IOS performance. Building on the design of an existing scanner, a new scanning head was developed to integrate with a compressed airflow system. This new design aimed to help the IOS capture high-precision data on surfaces where liquid-interference, such as saliva, might otherwise degrade scanning accuracy. The traditional scanning method, without airflow assistance, was employed as a control group for comparison. The study included five groups in total, one control group and four experimental groups, to investigate the effects of scanning lens obstruction, airflow presence, liquid media, and the use of the new scanning head on scanning process and accuracy. Each group underwent 15 scans, generating ample data for a robust statistical comparison. By evaluating trueness and precision in each group, the study assessed the impact of the compressed airflow system on the accuracy of IOS data collected from liquid-interference surfaces. Additionally, we selected Elite and Primescan scanners as references for numerical accuracy values.</p><p><strong>Results: </strong>The scanning accuracy on liquid-interference surfaces was significantly reduced in terms of both trueness and precision [Trueness: 18.5 (6.5) <i>vs</i>. 38.0 (6.7), <i>P</i> < 0.05; Precision: 19.1 (8.5) <i>vs</i>. 31.7 (15.0), <i>P</i> < 0.05]. The use of the new scanning head assisted by the compressed airflow system significantly improved the scanning accuracy [Trueness: 22.3(7.6) <i>vs</i>. 38.0 (6.7), <i>P</i> < 0.05; Precision: 25.8 (9.6) <i>vs</i>. 31.7 (15.0), <i>P</i> < 0.05].</p><p><strong>Conclusion: </strong>The scanning head based on the compressed airflow system can assist in improving the accuracy of data obtained from liquid-interference surfaces by the IOS.</p>","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"57 1","pages":"121-127"},"PeriodicalIF":0.0,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11759805/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Microneedle combined with photodynamic therapy in the treatment of oral leukoplakia].","authors":"Ying Han, Pu Zhao, Hongwei Liu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To explore whether microneedle pretreatment can significantly improve the efficacy and safety of 5-aminolevulinic acid (ALA)-photodynamic therapy (PDT) in the treatment of oral leukoplakia.</p><p><strong>Methods: </strong>A non-randomized controlled clinical trial was conducted. Patients with clinical and pathological diagnosis of oral leukoplakia in the Department of Oral Mucosa, Peking University School and Hospital of Stomatology were divided into experimental group and control group. The control group was treated with conventional ALA-PDT, and the experimental group was pretreated with micro- needle buckling under superficial anesthesia with lidocaine before conventional ALA-PDT. The clinical manifestations of the two groups were recorded, the lesion area was measured, the clinical efficacy was evaluated, the number of treatment sessions and treatment unit duration were analyzed, and the pain after treatment was evaluated by visual analogue scale. The above data of the two groups were statistically analyzed.</p><p><strong>Results: </strong>A total of 11 patients were included in the experimental group and 19 patients were included in the control group. The complete remission rate of the experimental group and the control group was 45.5% and 36.8%, the partial remission rate was 54.5% and 57.9%, and the no remission rate was 0% and 5%, respectively. There was no significant difference in the treatment effect between the two groups. Meanwhile, the treatment unit duration of the experimental group and the control group were (9.05±5.74) min/cm² and (21.38±15.44) min/cm², respectively, and the number of treatment sessions were (2.36±0.67) times and (3.58±1.57) times, respectively. These differences between the two groups were statistically significant (<i>t</i>=-3.125, <i>P</i> < 0.05; <i>t</i>=-2.932, <i>P</i> < 0.05). Similarly, multiple linear regression analysis with 7 factors including age, dysplastic pathology, lesion classification, etc., also confirmed that pretreatment could significantly shorten the treatment unit duration (<i>P</i> < 0.05). In addition, there was no significant difference in pain score (visual analogue scale) between the two groups after treatment, and the microneedle puncture pretreatment did not increase the adverse reactions of ALA-PDT treatment.</p><p><strong>Conclusion: </strong>Microneedle pretreatment followed by conventional ALA-PDT shows a good clinical effect on oral leukoplakia, which can significantly shorten the clinical treatment time, reduce the number of visits, and save medical costs.</p>","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"57 1","pages":"91-96"},"PeriodicalIF":0.0,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11759803/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Clinical application and three-dimensional finite element analysis of along-axis extraction method in mandibular mesial and horizontally impacted third molar surgery].","authors":"Fei Wang, Xinyue Zhang, Muqing Liu, Enbo Wang, Denghui Duan","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the clinical application effect of the along-axis extraction method in the extraction of impacted mandibular third molars (IMTM) and to compare the biomechanical characteristics of different root extraction techniques through three-dimensional finite element analysis.</p><p><strong>Methods: </strong>A total of 68 patients requiring IMTM extraction were enrolled and randomly divided into two groups: the experimental group underwent the along-axis extraction method, while the control group underwent the traditional buccal bone removal and root extraction method. The duration of the procedure, intraoperative and postoperative complications were recorded. Three-dimensional finite element analysis further revealed the stress distribution in the tooth root, jawbone, periodontal ligament, and mandibular canal during different root extraction methods.</p><p><strong>Results: </strong>The duration of root extraction, pain score and swelling on the first postoperative day in the control group were (7.87±3.90) min, 4.62±1.90 and (11.37±5.12) mm, respectively, which were significantly higher than those in the experimental group [(5.74±2.37) min, 3.87±1.19 and (7.22±3.39) mm, respectively]. The root fracture rate and lingual bone plate fracture rate in the control group were significantly higher than those in the experimental group (<i>P</i> < 0.05). The results of finite element analysis showed that the control group ' s lingual al-veolar bone had the higher peak equivalent stress, and lingual bone plate fracture was prone to occur. The periodontal ligament of the experimental group had the higher equivalent stress value, making it more likely to rip and more likely to cause root displacement. When subjected to force, the experimental group' s instantaneous root displacement was higher, but the control group ' s root displacement was more pronounced in the lingual direction.</p><p><strong>Conclusion: </strong>This study suggests that the along-axis extraction me-thod can not only effectively shorten the operative time but also reduce postoperative complications after extraction of impacted mandibular third molars, and enhance the safety of the operation and the patient' s comfort. Three-dimensional finite element analysis shows the biomechanical characteristics of various root extraction techniques visually, serves as a valuable guide for choosing and refining clinical surgical techniques, and confirms that extracting a tooth' s root along its long axis yields better clinical results.</p>","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"57 1","pages":"106-112"},"PeriodicalIF":0.0,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11759807/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of LncRNA SNHG20 on epithelial mesenchymal transition and microtubule formation in human oral squamous cell carcinoma cells through targeted regulation of the miR-520c-3p/<i>RAB22A</i> pathway].","authors":"Minying Ma, Xiaoqin Chao, Yang Zhao, Guoting Zhao","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effects of LncRNA SNHG20 on epithelial mesenchymal transition (EMT) and microtubule formation in human oral squamous cell carcinoma (OSCC) cells through targeted regulation of the miR-520c-3p/<i>RAB22A</i> pathway.</p><p><strong>Methods: </strong>After real-time fluorescence quantitative detection of LncRNA SNHG20, miR-520c-3p, <i>RAB22A</i> mRNA expression levels in OSCC tissues and cells, dual luciferase reporter assay was used to detect the relationship between the three. OSCC cells were randomly separated into control group, sh-NC group, sh-SNHG20 group, sh-SNHG20+anti NC group, and sh-SNHG20+anti miR-520c-3p group. Western blotting was used to detect the expression of N-cadherin, vimentin, and E-cadherin proteins in the OSCC cells. The morphology of HSC-3 cells was observed under microscope. Changes in the number of microtubules formed were detected. The effect of LncRNA SNHG20 on the growth of OSCC tumors and the expression levels of LncRNA SNHG20, miR-520c-3p and RAB22 A in the transplanted tumors were detected by nude mice tumorigenesis experiment.</p><p><strong>Results: </strong>LncRNA SNHG20 and <i>RAB22A</i> mRNA were upregulated in the OSCC tissues and cells, while miR-520c-3p was downregulated (<i>P</i> < 0.05). There were binding sites between LncRNA SNHG20 and miR-520c-3p, RAB22A and miR-520c-3p, which had targeted regulation relationship. Compared with the sh-NC group, the sh-SNHG20 group had fewer stromal like cells, more epithelial like cells, incomplete microtubule structure, and fewer nodules. LncRNA SNHG20, RAB22A, N-Cadherin, and vimentin were downregulated, while miR-520c-3p and E-cadherin were upregulated (<i>P</i> < 0.05). Compared with the sh-SNHG20+anti-NC group, the sh-SNHG20+anti-miR-520c-3p group had a higher number of stromal like cells, a lower number of epithelioid cells, tighter microtubule arrangement, and more microtubule nodules. miR-520c-3p and E-cadherin were downregulated, while RAB22A, N-cadherin, and vimentin were upregulated (<i>P</i> < 0.05). The transplanted tumor of OSCC in sh-SNHG20 group was smaller and lower than that in sh-NC group. The expression levels of LncRNA SNHG20 and RAB22A in the transplanted tumor tissues were lower than those in sh-NC group, and the expression level of miR-520c-3p was higher than that in sh-NC group (<i>P</i> < 0.05).</p><p><strong>Conclusion: </strong>LncRNA SNHG20 promotes epithelial-mesenchymal transition and microtubule formation in human oral squamous cell carcinoma cells by targeting the miR-520c-3p/<i>RAB22A</i> pathway. Inhibiting the expression of LncRNA SNHG20 can target and regulate the miR-520c-3p/<i>RAB22A</i> pathway to inhibit EMT and microtubule formation in OSCC cells.</p>","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"57 1","pages":"26-32"},"PeriodicalIF":0.0,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11759804/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Factors associated with spontaneous re-eruption of traumatically intruded permanent anterior teeth in children and adolescents].","authors":"Minting Deng, Nan Wang, Bin Xia, Yuming Zhao, Junxia Zhu","doi":"","DOIUrl":"","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To analyze the factors related to spontaneous re-eruption after intruded injury in permanent anterior teeth in children and adolescents.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Clinical data from 5- to 17-year-old patients who sustained intrusive luxation of permanent anterior teeth and treated in the Department of Pedia-tric Dentistry of Peking University School and Hospital of Stomatology from June 2015 to August 2024 were reviewed. Information of age, gender, degree of intrusion, direction of intrusion, tooth development, concomitant injuries, luxation and post-osteoclastic eruption of the adjacent teeth were recorded. The patients were divided into two groups based on whether they showed spontaneous re-eruption during advised observation after intrusion. Univariate and multifactor analysis were performed using Logistic regression.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Data from 170 teeth in 139 patients whose age ranging from 5.3-16.3 years [mean age (9.0± 2.1) years] were examined. A gender disparity was observed among the patients, with 84 being male and 55 being female. Among the 170 teeth, 112 were categorized as successfully spontaneous re-eruption during advised observation after intrusion, while 58 were not. In terms of the degree of intrusion, 45 teeth (26.47%) had intrusion less than 3 mm, 102 teeth (60.00%) experienced intrusion between 3-7 mm, and 23 teeth (13.53%) were faced with intrusion exceeding 7 mm. As for the direction of intrusion, 117 teeth (68.82%) were straight intrusion while mesial-distal and buccal-lingual intrusion respectively accounting for 17 (10.00%) and 23 (13.53%). Multivariate Logistic regression analysis showed that mesial-distal intrusion (&lt;i&gt;OR&lt;/i&gt;=0.167, 95%&lt;i&gt;CI&lt;/i&gt;: 0.031-0.9048, &lt;i&gt;P&lt;/i&gt;=0.038), intrusion of &gt;7 mm (&lt;i&gt;OR&lt;/i&gt;=0.065, 95%&lt;i&gt;CI&lt;/i&gt;: 0.014-0.299, &lt;i&gt;P&lt;/i&gt; &lt; 0.001) and luxation of adjacent teeth (&lt;i&gt;OR&lt;/i&gt;=0.369, 95%&lt;i&gt;CI&lt;/i&gt;: 0.144-0.944, &lt;i&gt;P&lt;/i&gt;=0.037) were independent risk factors for spontaneous re-eruption of traumatically intruded permanent anterior teeth in children and adolescents during advised observation after intrusion, while intrusion of &lt; 3 mm (&lt;i&gt;OR&lt;/i&gt;=9.860, 95%&lt;i&gt;CI&lt;/i&gt;: 2.430-40.009, &lt;i&gt;P&lt;/i&gt;=0.001) and post-osteoclastic eruption of adjacent teeth (&lt;i&gt;OR&lt;/i&gt;=4.712, 95%&lt;i&gt;CI&lt;/i&gt;: 1.528-14.531, &lt;i&gt;P&lt;/i&gt;=0.007) were independent protective factors. The possibility of spontaneous re-eruption in permanent anterior teeth during advised observation after intrusion was decreased by 61.1% with the increase of root development using Cvek' s classification (&lt;i&gt;OR&lt;/i&gt;=0.611, 95%&lt;i&gt;CI&lt;/i&gt;: 0.408-0.914, &lt;i&gt;P&lt;/i&gt;=0.017). Age (&lt;i&gt;OR&lt;/i&gt;=1.077, 95%&lt;i&gt;CI&lt;/i&gt;: 0.763-1.521, &lt;i&gt;P&lt;/i&gt;=0.673) and laceration of gingival (&lt;i&gt;OR&lt;/i&gt;=0.865, 95%&lt;i&gt;CI&lt;/i&gt;: 0.290-2.578, &lt;i&gt;P&lt;/i&gt;=0.794) didn't significantly affect the spontaneous re-eruption during advised observation after intrusion.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;In this study, mesial-distal intrusion, intrusion of &gt;7 mm and luxat","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"57 1","pages":"148-153"},"PeriodicalIF":0.0,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11759786/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Knockdown of <i>NPTX1</i> promotes osteogenic differentiation of human bone marrow mesenchymal stem cells].","authors":"Ting Shuai, Yanyan Guo, Chunping Lin, Xiaomei Hou, Chanyuan Jin","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To initially investigate the function of neuronal pentraxin 1 (<i>NPTX1</i>) gene on osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).</p><p><strong>Methods: </strong>hBMSCs were induced to undergo osteogenic differentiation, and then RNA was collected at different time points, namely 0, 3, 7, 10 and 14 d. The mRNA expression levels of key genes related with osteogenic differentiation, including runt-related transcription factor 2 (<i>RUNX2</i>), alkaline phosphatase (<i>ALP</i>), osteocalcin (<i>OCN</i>), and <i>NPTX1</i>, were detected on the basis of quantitative real-time polymerase chain reaction (qPCR) technology. In order to establish a stable <i>NPTX1</i>-knockdown hBMSCs cell line, <i>NPTX1</i> shRNA lentivirus was constructed and used to infect hBMSCs. ALP staining, alizarin red (AR) staining, and qPCR were employed to assess the impact of <i>NPTX1</i>-knockdown on the osteogenic differentiation ability of hBMSCs.</p><p><strong>Results: </strong>The results showed that during the osteogenic differentiation of hBMSCs <i>in vitro</i>, the mRNA expression levels of osteogenic genes <i>RUNX2</i>, <i>ALP</i> and <i>OCN</i> significantly increased compared with 0 d, while <i>NPTX1</i> expression decreased markedly (<i>P</i> < 0.01) as the osteogenic induction period exten-ded. At 72 h post-infection with lentivirus, the result of qPCR indicated that the knockdown efficiency of <i>NPTX1</i> was over 60%. After knocking down <i>NPTX1</i> in hBMSCs, RNA was extracted from both the <i>NPTX1</i>-knockdown group (sh <i>NPTX1</i> group) and the control group (shNC group) cultured in regular proliferation medium. The results of qPCR showed that the expression levels of osteogenic-related genes <i>RUNX2</i> and osterix (<i>OSX</i>) were significantly higher in the sh <i>NPTX1</i> group compared with the shNC group (<i>P</i> < 0.01). ALP staining revealed a significantly deeper coloration in the sh <i>NPTX1</i> group than in the shNC group at the end of 7 d of osteogenic induction. AR staining demonstrated a marked increase in mineralized nodules in the sh <i>NPTX1</i> group compared with the shNC group at the end of 14 d of osteogenic induction.</p><p><strong>Conclusion: </strong><i>NPTX1</i> exerts a modulatory role in the osteogenic differentiation of hBMSCs, and its knockdown has been found to enhance the osteogenic differentiation of hBMSCs. This finding implies that <i>NPTX1</i> could potentially serve as a therapeutic target for the treatment of osteogenic abnormalities, including osteoporosis.</p>","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"57 1","pages":"7-12"},"PeriodicalIF":0.0,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11759797/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Severe malnutrition during pregnancy complicated with acute pyelonephritis causing sepsis, refractory septic shock and multiple organ failure: A case report].","authors":"Fangfei Xie, Hong Qiao, Boya Li, Cui Yuan, Fang Wang, Yu Sun, Shuangling Li","doi":"","DOIUrl":"","url":null,"abstract":"&lt;p&gt;&lt;p&gt;This study reports the diagnosis and treatment of a 26-year-old pregnant woman with severe malnutrition combined with acute pyelonephritis causing sepsis, refractory septic shock and multiple organ failure. A female patient, 26 years old, was admitted to hospital mainly due to \"menelipsis for more than 19 weeks, nausea and vomiting for 20 days, fever with fatigue for 3 days\". At the end of 19 weeks of intrauterine pregnancy, the patient presented with fever accompanied by urinary tract irritation. Laboratory tests showed elevated inflammatory indicators, and ultrasonography showed bilateral pelvicalyceal dilation. She was diagnosed with acute pyelonephritis, sepsis, acute kidney injury (AKI) and severe malnutrition. After a whole-hospital consultation, the patient was treated with meropenem and vancomycin as antimicrobial therapy, and bilateral nephrostomy drainage was performed simultaneously. After that, the patient suffered a sudden decrease in blood pressure, blood oxygen saturation, and rapid heart rate. Septic shock with multiple organ dysfunction was considered, and she was transferred to intensive care unit (ICU) immediately. After the patient was transferred to ICU, emergency tracheal intubation and ventilator-assisted ventilation were performed. Rapid fluid resuscitation was administered for the patient. While pulse indicator continuous cardiac output (PICCO) monitoring was performed, norepinephrine, terlipressin, and methylene blue were administered to maintain peripheral vascular resistance. Since the patient developed septic cardiomyopathy and cardiogenic shock later, levosimendan and epinephrine were admi-nistered to improve cardiac function. While etiological specimens were delivered, meropenem, teicoplanin and caspofungin were given as initial empiric antimicrobial therapy. Unfortunately, the intrauterine fetal death occurred on the night of admission to ICU. On the 3rd day of ICU admission, a still-born child was delivered vaginally with 1/5 defect of the fetal membrane. On the 6th day of ICU admission, the patient had fever again with elevated inflammatory indicators. After excluding infection in other parts, intrau-terine infection caused by incomplete delivery of fetal membrane was considered. Then emergency uterine curettage was performed and the infection gradually improved. Later the laboratory results showed that the nephrostomy drainage was cultured for &lt;i&gt;Escherichia coli&lt;/i&gt; and uterine, cervical and vaginal secretions were cultured for &lt;i&gt;Candida albicans&lt;/i&gt;. Due to severe infection and intrauterine incomplete abortion, the patient developed disseminated intravascular coagulation (DIC). Active antimicrobial therapy and blood product supplement were given. However, the patient was critically ill with significant decrease in hemoglobin and platelets combined with multiple organ failure. Thrombotic microangiopathy (TMA) was not excluded yet, so plasma exchange was performed for the patient in order not to delay tre","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"57 1","pages":"202-207"},"PeriodicalIF":0.0,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11759789/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Therapeutic effect of concentrated growth factors combined with self-curing calcium phosphate cement on periodontal intrabony defects: Clinical and radiographic evaluation].","authors":"Xinying Wang, Xueyuan Cheng, Yong Zhang, Fei Li, Jinyu Duan, Jing Qiao","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To clarify the role of concentrated growth factors (CGF) in the treatment of periodontal cement defects using calcium phosphate cement (CPC) with self-curing properties.</p><p><strong>Methods: </strong>Thirty-six intrabony defects were randomly divided into two groups. The experimental group received CGF+CPC treatment (<i>n</i>=18), while the control group received CPC treatment alone (<i>n</i>=18). The probing depth, clinical attachment loss, and hard tissue filling as measured by cone beam CT (CBCT) were evaluated at baseline and 1 year postoperatively in both groups, and the levels of major growth factors in CGF and serum were compared [platelet-derived growth factor-BB (PDGF-BB), transforming growth factor-β1 (TGF-β1), insulin-like growth factor-1 (IGF-1), and vascular endothelial growth factor (VEGF)].</p><p><strong>Results: </strong>At baseline, there were no statistically significant differences in probing depth, clinical attachment loss and CBCT measurements between the two groups (<i>P</i>>0.05). At 1 year postoperatively, significant improvements were observed in parameters mentioned above in both groups (<i>P</i> < 0.05). The CGF+CPC group seemed more effective compared with the CPC group in reduction of probing depth [(4.5±1.3) mm <i>vs.</i> (3.2±1.1) mm] and clinical attachment gain [(3.8±0.9) mm <i>vs.</i> (2.0±0.5) mm, <i>P</i> < 0.05]. Compared with the group treated with CPC alone, the hard tissue filling degree shown by CBCT in the CGF+CPC group was significantly increased [the reduction of the depth of the intrabony defects was (3.9±1.2) mm <i>vs.</i> (2.1±0.7) mm, respectively, <i>P</i> < 0.01]. At 1 year post-operatively, the volume of the intrabony defects shown by CBCT in the CGF+CPC group was reduced by (0.031 8±0.004 1) mL, which was significantly more than that in the CPC group [(0.019 7±0.001 2) mL, <i>P</i> < 0.05]. In addition, the concentration of the main growth factors (PDGF-BB, TGF-β1, IGF-1, and VEGF) in CGF were higher than those in serum (<i>P</i> < 0.001).</p><p><strong>Conclusion: </strong>After 1 year of follow-up, the results of the present study indicated that CGF could significantly improve the clinical and radiological effects of CPC on the treatment of periodontal intrabony defects.</p>","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"57 1","pages":"42-50"},"PeriodicalIF":0.0,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11759798/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}