北京大学学报（医学版）最新文献

{"title":"[Expression and regulatory mechanism of miR-34a in neonatal rat model of bron-chopulmonary dysplasia induced by hyperoxia].","authors":"Mengyue Huo, Hua Mei, Yuheng Zhang, Yanbo Zhang, Chunli Liu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the expression and possible regulatory mechanism of miR-34a in the lung tissue of neonatal rat model of bronchopulmonary dysplasia (BPD) induced by hyperoxia.</p><p><strong>Methods: </strong>In the study, 80 newborn SD rats were randomly divided into hyperoxia group (FiO₂=60%) and air group (FiO₂=21%) within 2 hours after birth, 40 rats per group. Lung tissue samples of the SD rats in each group were extracted on the 1st, 7th, 14th and 21st days after birth, and the pathological changes of lung tissue were observed under light microscope after HE staining. The number of radial alveolar counts (RAC) and the mean alveolar diameter (MAD) and the thickness of alveolar septal thickness (AST) were measured to evaluate the development of alveoli. Real-time fluorescence quantitative PCR was used to detect the expression of miR-34a, angiopoietin-1 (<i>Ang</i>-<i>1</i>) and tyrosine kinase receptor-2 (<i>Tie</i>-<i>2</i>) in lung tissue of rats in hyperoxia group and air group at different time points. Enzyme-linked immunosorbent assay (ELISA) was used to detect the proteins expression of Ang-1 and Tie-2 in the lung tissues of the two groups at different time points.</p><p><strong>Results: </strong>The weight of rats in the hyperoxia group on the 7th, 14th and 21st days after birth was significantly lower than that in the air group (<i>P</i> all < 0.05). With the prolongation of oxygen exposure, the number of alveoli decreased, the volume increased, the structure simplified, the alveolar cavity enlarged obviously and the alveolar septum thickened in the hyperoxia group. On the 7th, 14th and 21st days after birth, the RAC in the hyperoxia group was significantly lower than that in the air group (<i>P</i> all < 0.05). Compared with the air group, MAD and AST increased significantly on the 7th, 14th and 21st days after birth in the hyperoxia group, and the difference was statistically significant (<i>P</i> all < 0.05). The expression level of miR-34a in lung tissue of hyperoxia group was significantly higher than that of air group on the 7th, 14th and 21st days after birth, and the difference was statistically significant (<i>P</i> all < 0.05). Compared with the air group at the same time point, the expression levels of Ang-1 and Tie-2 mRNA and protein in the hyperoxia group were lower than those in the air group on the 14th and 21st days after birth (<i>P</i> all < 0.05).</p><p><strong>Conclusion: </strong>The new BPD model of newborn SD rats can be successfully established by continuous exposure to 60% hyperoxia. The expression of miR-34a was up-regulated in the lung tissue of the new BPD model of neonatal rats. MiR-34a may play an important role in the occurrence and development of BPD by regulating Ang-1/Tie-2 signal pathway.</p>","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"57 2","pages":"237-244"},"PeriodicalIF":0.0,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11992441/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Predictive value of coronary microcirculation dysfunction after revascularization in patients with acute myocardial infarction for acute heart failure during hospitalization].","authors":"Lan Wang, Yuliang Ma, Weimin Wang, Tiangang Zhu, Wenying Jin, Hong Zhao, Chengfu Cao, Jing Wang, Bailin Jiang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To study incident and clinical characteristics of the coronary microcirculation dysfunction (CMD) in patients with acute myocardial infarction (AMI) after percutaneous coronary intervention (PCI) by myocardial contrast echocardiography (MCE) and to explore the predictive value of CMD for in-hospital acute heart failure event.</p><p><strong>Methods: </strong>One hundred and forty five patients with AMI who had received PCI and completed MCE during hospitalization in Peking University People' s Hospital from November 2015 to July 2021 were enrolled in our study. The patients were divided into CMD group and normal group according to the coronary microcirculation status detected by MCE. Clinical data and MCE data of the two groups were collected and analyzed. The acute heart failure event during hospitalization was described. A multivariate Logistic regression model was built to analyze the risk of acute heart failure in patients with CMD. A receiver operating characteristic (ROC) curve was drawn to evaluate the value of CMD in predicting acute heart failure event.</p><p><strong>Results: </strong>CMD detected by MCE occurred in 87 patients (60%). Compared with normal group, patients with CMD had higher troponin I (TnI) peak level [52.8 (8.1, 84.0) μg/L <i>vs</i>. 18.9 (5.7, 56.1) μg/L, <i>P</i>=0.005], poorer Killip grade on admission (<i>P</i>=0.030), different culprit vessel (<i>P</i> < 0.001) and more patients had thrombolysis in myocardial infarction (TIMI) flow pre-PCI less than grade 3 in culprit vessel (65.1% <i>vs</i>. 43.1%, <i>P</i>=0.025). Meanwhile, patients with CMD had poorer left ventricular ejection fraction (LVEF) [52% (43%, 58%) <i>vs</i>. 61% (54%, 66%)], poorer global longitudinal strain (GLS) [-11.2% (-8.7%, -14.0%) <i>vs</i>.-13.9% (-10.8%, -17.0%)] and worse wall motion score index (WMSI) (1.58±0.36 <i>vs</i>. 1.25± 0.24) (<i>P</i> all < 0.001). Acute left heart failure happened in 13.8% of the CMD patients, which were significant higher than that in the patients with normal coronary microcirculation perfusion (1.7%, <i>P</i>=0.013). After correcting for the culprit vessel, the TIMI flow pre-PCI in the culprit vessel and the peak TnI value, the risk of acute left heart failure in the patients with CMD was still high (<i>OR</i>=9.120, 95%<i>CI</i>: 1.152-72.192, <i>P</i>=0.036). The area under ROC curve (AUC) was 0.677 (95%<i>CI</i>: 0.551-0.804, <i>P</i>=0.035).</p><p><strong>Conclusion: </strong>The incidence of CMD detected by MCE in patients with AMI post-PCI was 60%. Patients with CMD have a higher risk of acute left heart failure during hospitalization.</p>","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"57 2","pages":"267-271"},"PeriodicalIF":0.0,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11992466/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Correlation between urination intermittences and urodynamic parameters in benign prostatic hyperplasia patients].","authors":"Ning Liu, Libo Man, Feng He, Guanglin Huang, Jianpo Zhai","doi":"","DOIUrl":"","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To explore the impact factors and the clinical significance of the urination intermittences in benign prostatic hyperplasia (BPH) patients.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;A retrospective study was performed in BPH patients who underwent urodynamic studies in Beijing Jishuitan Hospital form January 2016 to June 2021. The patients were aged 45 to 84 years with a median age of 63 years, and all the patients had no previous history of neurological disease and had no positive findings in neurological examinations. All the patients had free uroflometry followed by urethral catheterization and urodynamic tests. The voiding work of bladder was calculated using the detrusor power curve method, and the voiding power of bladder and the voiding energy consumption were also calculated. The frequency of urination intermittences generated in uroflometry was also recorded and the patients were divided into different groups according to it. The detrusor pressure at maximal flow rate (P&lt;sub&gt;det&lt;/sub&gt;Q&lt;sub&gt;max&lt;/sub&gt;), the maximal flow rate (Q&lt;sub&gt;max&lt;/sub&gt;), the bladder contractile index (BCI), the bladder outlet obstruction index (BOOI), the voiding work, the voiding power, and the voiding energy consumption were compared among the different groups. Multiva-riate analyses associated with presence of urination intermittences were performed using step-wise Logistic regressions.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;There were 272 patients included in this study, of whom, 179 had no urination intermittence (group A), 46 had urination intermittence for only one time (group B), 22 had urination intermittence for two times (group C), and 25 had urination intermittence for three times and more (group D). The BCI were 113.4±28.2, 101.0±30.2, 83.3±30.2, 81.0±30.5 in groups A, B, C, and D, respectively; The voiding power were (29.2±14.8) mW, (16.4±9.6) mW, (14.5±7.1) mW, (8.5±5.0) mW in groups A, B, C, and D, respectively, and the differences were significant (&lt;i&gt;P&lt;/i&gt; &lt; 0.05). The BOOI were 41.6±29.3, 46.4±31.0, 41.4±29.0, 42.7±22.8 in groups A, B, C, and D, respectively; The voiding energy consumption were (5.41±2.21) J/L, (4.83±2.31) J/L, (5.02±2.54) J/L, (4.39±2.03) J/L in groups A, B, C, and D, respectively, and the differences were insignificant (&lt;i&gt;P&lt;/i&gt;&gt;0.05). Among the patients, 179 cases were negative in presence of urination intermittences and 93 cases were positive. Step-wise Logistic regression analysis showed that bladder power (&lt;i&gt;OR&lt;/i&gt;=0.814, 95%&lt;i&gt;CI&lt;/i&gt;: 0.765-0.866, &lt;i&gt;P&lt;/i&gt; &lt; 0.001), BCI (&lt;i&gt;OR&lt;/i&gt;=1.023, 95%&lt;i&gt;CI&lt;/i&gt;: 1.008-1.038, &lt;i&gt;P&lt;/i&gt;=0.003), and bladder work (&lt;i&gt;OR&lt;/i&gt;=2.232, 95%&lt;i&gt;CI&lt;/i&gt;: 1.191-4.184, &lt;i&gt;P&lt;/i&gt;=0.012) were independent risk factors for urination intermittences in the BPH patients.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;The presence of urination intermittences in the BPH patients was mainly influenced by bladder contractile functions, and was irrelevant to the degree of bladder outlet obstruction. The increase","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"57 2","pages":"328-333"},"PeriodicalIF":0.0,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11992446/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144062067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Observation of the efficacy of dupilumab for treatment of atopic dermatitis in the elderly].","authors":"Ran Sun, Yuhao Wu, Mei DI, Xiaoyang Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the efficacy and safety of dupilumab in the treatment of atopic dermatitis in the elderly.</p><p><strong>Methods: </strong>In this study, elderly patients with atopic dermatitis treated with dupilumab for at least 16 weeks in the Department of Dermatology and Venereology of Beijing Anzhen Hospital from January 2021 to October 2023 were retrospectively collected. Clinical indicators were compared before, during and after treatment, including pruritus numerical rating score (PNRS), eczema area and severity index (EASI), dermatology life quality index (DLQI) score, and the incidence of adverse events was recorded. The expression of interferon γ (IFN-γ), interleukin-4 (IL-4) and interleukin-6 (IL-6) in peripheral blood were compared before treatment and after 16 weeks of treatment.</p><p><strong>Results: </strong>A total of 90 elder patients with atopic dermatitis were included, EASI, PNRS and DLQI scores all showed a gradual downward trend during the treatment period, which was manifested as a rapid decline in the first 4 weeks after starting treatment, and then the decline gradually leveled off. The results of point-to-point comparison showed that EASI, PNRS and DLQI scores in 4 weeks after treatment were significantly lower than those before treatment (<i>P</i> < 0.001); In the 16th week after treatment, the scores of the above therapeutic indicators were further reduced, and the difference was statistically significant compared with the 4th week (<i>P</i> < 0.01); The EASI score was significantly lower at each time point than the previous time point, indicating that the patients' skin lesions continued to improve significantly. The overall efficacy of dupliumab was evaluated. After 4 weeks of treatment, 62.89% of patients achieved EASI-50 (EASI score decreased by ≥50%), and 74.4% of patients' DLQI score decreased by ≥4 points. After 16 weeks of treatment, 57.8% of the patients achieved EASI-75, 32.2% achieved EASI-90, and the PNRS and DLQI scores of all the patients decreased by ≥4 points. After 16 weeks of treatment, the expression levels of IL-4 and IL-6 were (31.62±6.23) ng/L and (14.36±2.25) ng/L, respectively, which were significantly lower than those before treatment (<i>P</i> < 0.001), and the expression level of IFN-γ was (15.37±3.14) ng/L, which was higher than before treatment (<i>P</i> < 0.001).The main adverse reactions were conjunctivitis (2 cases), injection site reaction (3 cases) and multiple bacterial folliculitis of the back (2 cases), which could be alleviated by symptomatic treatment, and no serious adverse reactions occurred.</p><p><strong>Conclusion: </strong>Dupilumab has shown good efficacy in the treatment of elderly atopic dermatitis, which can effectively improve clinical symptoms such as itching and skin lesions, improve the quality of life of patients, and no serious adverse reactions occurred during treatment, so it is safe and worthy of clinical promotion.</p>","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"57 2","pages":"298-302"},"PeriodicalIF":0.0,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11992437/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143974834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Renal autotransplantation for the treatment of complex renal aneurysm in a child: A case report].","authors":"Lei Yu, Wenbo Yang, Yufan Yang, Qiang Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Renal autotransplantation (RA) offers significant technical advantages for the management of certain complex renal vascular diseases, such as complex renal aneurysms and renal artery malformations. This report describes a case of a 5-year-old child with a complex left renal artery aneurysm combined with multiple aneurysms. The child was admitted to Peking University People's Hospital in December 2023 due to a one-year history of intermittent abdominal pain, with an abdominal mass detected in the past month. Computed tomography angiography(CTA) revealed multiple vascular anomalies, including: (1) a left renal artery aneurysm, (2) an abdominal aortic aneurysm, and (3) a right iliac artery aneurysm. After a comprehensive evaluation of these findings, the surgical team developed a treatment plan that involved the excision of the left renal artery aneurysm, autotransplantation of the left kidney, and resection of the abdominal aortic aneurysm with an artificial vascular catheterization. During surgery, it was discovered that the left renal artery anatomy was highly complex. The artery had two primary branches, along with an additional polar artery located at the lower pole. The aneurysm was identified at the distal end of the renal artery trunk, with a pronounced bulging at the intersection between the main renal artery trunk and its secondary branches. Due to these structural complexities, the team decided to use an <i>ex vivo</i> surgical approach to repair the aneurysm. <i>Ex vivo</i> repair involves temporarily removing the kidney from the body to repair the renal artery aneurysm with enhanced precision, enabling the surgical team to meticulously reconstruct the complex vascular architecture without the constraints of <i>in vivo</i> manipulation. The <i>ex vivo</i> repair of the renal artery aneurysm was successful, allowing for accurate vascular reconstruction and avoiding potential intraoperative complications. Following the reconstruction, the kidney was autotransplanted back into the child's body, and blood flow was effectively restored to the organ. The therapeutic outcome was excellent, with the child experiencing no postoperative complications. The patient recovered well and was discharged from the hospital in stable condition. This case underscores the value of renal autotransplantation combined with <i>ex vivo</i> repair for pediatric patients with complicated renal artery aneurysms. Through this report, we aim to provide insights and considerations for the surgical treatment of similar cases in children with complex renal vascular anatomy.</p>","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"57 2","pages":"396-399"},"PeriodicalIF":0.0,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11992461/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143963910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Personalized mandibular reconstruction assisted by three-dimensional retrieval model based on fully connected neural network and a database of mandibles].","authors":"Shiyu Qiu, Yang Lian, Yifan Kang, Lei Zhang, Yiwang Cai, Xiaofeng Shan, Zhigang Cai","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To propose a new protocol for personalized mandibular reconstruction assisted by three-dimensional (3D) retrieval model based on fully connected neural network (FCNN) and a database of mandibles, and to verify clinical feasibility of the protocol.</p><p><strong>Methods: </strong>A database of mandibles of 300 normal northern Chinese Han people was established. On the basis of cephalometry, the mandible landmarks with good stability were further screened. Mandibular landmarks were selected and geometric features of the mandible were extracted. A 3D retrieval algorithm was developed, which could retrieve the mandible most similar to a given mandible from the database. A FCNN was built to train the algorithm to improve accuracy of the 3D retrieval model. Using Geomagic Control 2014 software, matching accuracy of the 3D retrieval model was based on aforementioned mandible database and algorithm. From December 2019 to March 2021, a total of 5 patients underwent personalized mandibular reconstruction assisted by a 3D retrieval model based on mandible database and FCNN in the Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology. The most similar mandible was retrieved from mandible database through 3D retrieval algorithm. It was used to restore the premorbid morphology of defect area and guide mandibular reconstruction. For the 5 patients, mandible was reconstructed with iliac flap. Virtual surgical plan was transformed using individual surgical guides.</p><p><strong>Results: </strong>Through screening, mandibular landmarks with high reproducibility and stability were identified and composed of mandibular landmarker protocols. After training, the average deviation between most similar mandible retrieved from the 300-case mandible database through 3D retrieval model based on FCNN and given mandible was (1.77±0.44) mm. And the root-mean-square deviation between the most similar mandible retrieved from the database and given mandible was (2.58±0.86) mm. The mandibular reconstruction surgery was successful in all the 5 patients. Their facial symmetry and occlusion were restored. All the patients were satisfied with postoperative appearance. The mean deviation between postoperative mandible and preoperative design was (0.98±0.17) mm. The area with a deviation ≤1 mm accounted for 61.34%±14. 13%, ≤2 mm accounted for 83.82%±7.35%, and ≤3 mm accounted for 93.94%± 2.87%.</p><p><strong>Conclusion: </strong>The personalized mandibular reconstruction assisted by 3D retrieval model based on the 300-case mandible database and FCNN is feasible clinically.</p>","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"57 2","pages":"360-368"},"PeriodicalIF":0.0,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11992464/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143974840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Arthroscopic tissue engineering scaffold repair for cartilage injuries].","authors":"Zhenlong Liu, Zhenchen Hou, Xiaoqing Hu, Shuang Ren, Qinwei Guo, Yan Xu, Xi Gong, Yingfang Ao","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To standardize the operative procedure for tissue-engineered cartilage repair, by demonstrating surgical technique of arthroscopic implantation of decalcified cortex-cancellous bone scaffolds, and summarizing the surgical experience of the sports medicine department team at Peking University Third Hospital.</p><p><strong>Methods: </strong>This article elaborates on surgical techniques and skills, focusing on the unabridged implantation technology and surgical procedure of decalcified cortex-cancellous bone scaffolds under arthroscopy: First, the patient was placed in the supine position. After anesthesia had been established, the surgeon established an arthroscope and explored the damaged area under the scope. After confirming the size and location of the injury site, the surgeon cleaned the damaged cartilage, and also trimmed the edges of the cartilage to ensure that the cut surface was smooth and stable. the surgeon performed the micro-fracture surgery in the area of cartilage injury, and then measured the size of the injured area under the scope. Next, the surgeon manually trimmed the tissue-engineered scaffold based on the measurements taken under the arthroscope, and then directly implanted the scaffold using a sleeve. A honeycomb-shaped fixator was used to implant absorbable nails to fix the scaffold. After the scaffold was installed, the knee was repeatedly flexed and extended for 10-20 times to ensure stability and range of motion. Finally, the arthroscope was withdrawn and the wound was closed.</p><p><strong>Results: </strong>Decalcified cortex-cancellous bone scaffolds possessed unparalleled advantages over synthetic materials in terms of morphology and biomechanics. The cancellous bone part of the scaffold provided a three-dimensional, porous space for cell growth, while the cortical bone part offered the necessary mechanical strength. The surgery was performed entirely under arthroscopy to minimize invasiveness to the patient. Absorbable pins were used for fixation to ensure the stability of the scaffold. This technique could effectively improve the prognosis of the patients with cartilage injuries and standardized the surgical procedures for arthroscopic tissue-engineered scaffold operations in the patients with cartilage damage.</p><p><strong>Conclusion: </strong>With the standard arthroscopic tissue-engineered scaffold repair technique, it is possible to successfully repair damaged cartilage, alleviate symptoms in the short term, and provide a more ideal long-term prognosis. The author and their team explain the surgical procedures for tissue-engineered scaffolds under arthroscopy, with the aim of guiding future clinical practice.</p>","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"57 2","pages":"384-387"},"PeriodicalIF":0.0,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11992452/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Comparison of two registration methods for constructing virtual craniodentofacial patients based on cone beam computed tomography images].","authors":"Jiahui Ye, Shimin Wang, Zixuan Wang, Yunsong Liu, Yuchun Sun, Hongqiang Ye, Yongsheng Zhou","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To compare the registration accuracy of cone beam computed tomography (CBCT) images while registering to virtual craniodentofacial patients based on soft tissue and the dentition registration method.</p><p><strong>Methods: </strong>Virtual dentofacial patients out of 13 selected participants who needed CBCT scanning were established by impression with a registered-block impression (RBI) based on digital dental images, three-dimensional (3D) facial images and maxillofacial CBCT images. CBCT images were processed in the Mimics software program, establishing the craniofacial virtual patients based on CBCT images (CCTs). Registration between virtual patients from RBI and CCT, using the soft tissue in lower half face (STE) and dentition (DTN) as the reference area, respectively, forming two kinds of virtual craniofacial patients based on digital dental images, 3D facial images and skeletal images of CBCT (hiding the soft tissue and dental casts from CBCT). Three-dimensional deviation analysis was performed in the upper half face and lower half face of facial images from CBCT between two kinds of virtual craniodentofacial patients and compared with 3D facial images from RBI and recorded as root mean square error (RMSE). Paired-<i>t</i> test was used to compare the deviations of RMSEs between the upper and lower half of the face and the upper half of the face of facial images from CCT, respectively, between the two kinds of virtual craniodentofacial patients based on STE and DTN methods.</p><p><strong>Results: </strong>Paired-<i>t</i> tests showed that there was no statistically significant difference between the upper and lower half faces of facial images from CCT between STE and DTN (<i>P</i>>0.05), but the deviation of RMSEs of the upper half face of facial images from CCT in STE was smaller than those in DTN [(1.696±0.420) mm <i>vs</i>. (1.752±0.424) mm, <i>P</i> < 0.01].</p><p><strong>Conclusion: </strong>The registration accuracy of CBCT registered in virtual craniodentofacial patients using soft tissue as the reference area was higher.</p>","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"57 2","pages":"354-359"},"PeriodicalIF":0.0,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11992456/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Clinical outcomes of partial sialoadenectomy for the treatment of benign tumors in the submandibular gland].","authors":"Yuanyuan Yang, Shanshan Zhang, Guangyan Yu, Huijun Yang, Hongyu Yang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the clinical outcomes and explore the application of partial sialoadenectomy for the treatment of benign tumors in the submandibular gland (SMG).</p><p><strong>Methods: </strong>Patients with pleomorphic adenoma of the SMG who underwent surgical treatment in the Department of Oral and Maxillofacial Surgery, Peking University Shenzhen Hospital, from October 2017 to February 2021, were enrolled and assessed in the follow-up. Fifteen patients underwent partial sialoadenectomy (PS group), and 18 patients underwent total sialoadenectomy (TS group). Postoperative salivary secretion, degree of dry mouth, appearance changes of the face and neck, nerve damage, and tumor recurrence were compared between the groups. The volume of the glands on the operated and contralateral sides of the patients in the PS group, the saliva flow rate, and their correlations, were also analyzed.</p><p><strong>Results: </strong>There was no recurrence during the follow-up period. The whole saliva flow rate at rest in the PS group was higher than that in the TS group [(2.15±1.10) g/5 min <i>vs</i>. (1.35±0.97) g/5 min, <i>t</i>=2.208, <i>P</i>=0.035)], while the stimulated saliva flow rate was not significantly different. The objective feeling of dry mouth, evaluated by visual analogue scale (VAS) score, was more obvious in the TS group than in the PS group (<i>Z</i>=-2.244, <i>P</i>=0.025). In the PS group, the resting saliva flow rate of the SMG on the operated side was lower than that on the contralateral side of the same patient [(0.92±0.40) g/5 min <i>vs</i>. (1.18±0.40) g/5 min, <i>t</i>=-2.821, <i>P</i>=0.014], however, in the cases whose remaining SMG was more than 80% of the contralateral side, the saliva flow rate of both sides was not significantly different (<i>t</i>=-0.027, <i>P</i>=0.980). There was no significant difference in the saliva flow rate per unit volume of the gland on either side (<i>t</i>=-0.015, <i>P</i>=0.989), and the saliva flow rate of the operated SMG was positively correlated with the volume of the remaining gland (<i>r</i>=0.750, <i>P</i>=0.012). The VAS scores for neck deformity were not significantly different between the two groups (<i>t</i>=-0.997, <i>P</i>=0.319). No symptoms of nerve injury occurred in either group.</p><p><strong>Conclusion: </strong>Partial sialoadenectomy in the SMG can safely remove benign tumors while preserving glandular secretory function, with fewer complications and improved quality of life.</p>","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"57 2","pages":"334-339"},"PeriodicalIF":0.0,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11992459/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143961425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Gene silencing of Nemo-like kinase promotes neuralized tissue engineered bone regeneration].","authors":"Mengdi Li, Lei Lei, Zhongning Liu, Jian Li, Ting Jiang","doi":"","DOIUrl":"","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To identify the role of gene silencing or overexpression of Nemo-like kinase (&lt;i&gt;NLK&lt;/i&gt;) during the process of neural differentiation of human mesenchymal stem cells (hBMSCs), and to explore the effect of &lt;i&gt;NLK&lt;/i&gt; downregulation by transfection of small interfering RNA (siRNA) on promoting neuralized tissue engineered bone regeneration.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;&lt;i&gt;NLK&lt;/i&gt;-knockdown hBMSCs were established by transfection of siRNA (the experimental group was transfected with siRNA silencing the &lt;i&gt;NLK&lt;/i&gt; gene, the control group was transfected with control siRNA and labeled as negative control group), and &lt;i&gt;NLK&lt;/i&gt;-overexpression hBMSCs were established using lentivirus vector transfection technique (the experimental group was infected with lentivirus overexpressing the &lt;i&gt;NLK&lt;/i&gt; gene, the control group was infected with an empty vector lentivirus and labeled as the empty vector group). After neurogenic induction, quantitative real-time polymerase chain reaction (qPCR) was used to detect the expression of neural-related gene, and Western blot as well as immunofluorescence staining about several specific neural markers were used to evaluate the neural differentiation ability of hBMSCs.6-week-old male nude mice were divided into 4 groups: ① β-tricalcium phosphate (β-TCP) group, ② β-TCP+ osteogenic induced hBMSCs group, ③ β-TCP+ siRNA-negative control (siRNA-NC) transfection hBMSCs group, ④ β-TCP+ siRNA-&lt;i&gt;NLK&lt;/i&gt; transfection hBMSCs group. Four weeks after the subcutaneous ectopic osteogenesis models were established, the osteogenesis and neurogenesis were detected by hematoxylin-eosin (HE) staining, Masson staining and tissue immunofluorescence assay. Statistical analysis was conducted by independent sample &lt;i&gt;t&lt;/i&gt; test.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;After gene silencing of &lt;i&gt;NLK&lt;/i&gt; by siRNA in hBMSCs, neural-related genes, including the class Ⅲ β-tubulin (&lt;i&gt;TUBB3&lt;/i&gt;), microtubule association protein-2 (&lt;i&gt;MAP2&lt;/i&gt;), soluble protein-100 (&lt;i&gt;S100&lt;/i&gt;), nestin (&lt;i&gt;NES&lt;/i&gt;), NG2 proteoglycan (&lt;i&gt;NG2&lt;/i&gt;) and calcitonin gene-related peptide (&lt;i&gt;CGRP&lt;/i&gt;), were increased significantly in &lt;i&gt;NLK&lt;/i&gt;-knockdown hBMSCs compared with the negative control group(&lt;i&gt;P&lt;/i&gt; &lt; 0.05), and the expression levels of TUBB3 and MAP2 of the &lt;i&gt;NLK&lt;/i&gt; silencing group were also increased. Oppositely, after &lt;i&gt;NLK&lt;/i&gt; was overexpressed using lentivirus vector transfection technique, &lt;i&gt;TUBB3&lt;/i&gt;, &lt;i&gt;MAP2&lt;/i&gt;, &lt;i&gt;S100&lt;/i&gt; and &lt;i&gt;NG2&lt;/i&gt; were significantly decreased in &lt;i&gt;NLK&lt;/i&gt;-overexpression hBMSCs compared with the empty vector group (&lt;i&gt;P&lt;/i&gt; &lt; 0.05), and the expression level of TUBB3 was also decreased. 4 weeks after the subcutaneous ectopic osteogenesis model was established, more mineralized tissues were formed in the β-TCP+ siRNA-&lt;i&gt;NLK&lt;/i&gt; transfection hBMSCs group compared with the other three groups, and the expression of BMP2 and S100 was higher in the β-TCP+ siRNA-&lt;i&gt;NLK&lt;/i&gt; transfection hBMSCs group than in th","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"57 2","pages":"227-236"},"PeriodicalIF":0.0,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11992439/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143973526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}