Bing du xue bao = Chinese journal of virology最新文献_第10页

[Proinflammatory Cytokine and Chemokine Production of Mouse Macrophages in Response to EV-A71 Infection]. 小鼠巨噬细胞对EV-A71感染的促炎细胞因子和趋化因子产生[j]。

Bing du xue bao = Chinese journal of virology Pub Date : 2016-05-01

Guangcheng Xie, Nijun Guo, Ying Wang, Yongkang Zhou, Dandi Li, Miao Jin, Lili Pang, Xiaoman Sun, Qing Zhang, Zhaojun Duan

引用次数: 0

[Complete Genome Analysis of a Recombinant GI Norovirus Identified in Huzhou,China]. 中国湖州一株重组胃肠道诺如病毒的全基因组分析

Bing du xue bao = Chinese journal of virology Pub Date : 2016-05-01

Lei Ji, Liping Chen, Xiaofang Wu, Deshun Xu, Jiankang Han

引用次数: 0

[Research Progress in Enveloped Glycoproteins and the Membrane-fusion Mechanism of Nipah Virus]. 尼帕病毒包膜糖蛋白与膜融合机制的研究进展

Bing du xue bao = Chinese journal of virology Pub Date : 2016-05-01

Dong Yan, Zhiyu Wang

引用次数: 0

[Functional Analyses of HIV-1 Specific Cytotoxic T Lymphocyte Clones]. HIV-1特异性细胞毒性T淋巴细胞克隆的功能分析

Bing du xue bao = Chinese journal of virology Pub Date : 2016-05-01

Cong Jin, Ting Tu, Danlei Mou, Qunhui Li, Xing Song, Haixia Zhang, Tao Tang, Tao Li, Hao Wu, Huabiao Chen

{"title":"[Functional Analyses of HIV-1 Specific Cytotoxic T Lymphocyte Clones].","authors":"Cong Jin, Ting Tu, Danlei Mou, Qunhui Li, Xing Song, Haixia Zhang, Tao Tang, Tao Li, Hao Wu, Huabiao Chen","doi":"","DOIUrl":"","url":null,"abstract":"Human immunodeficiency virus type 1(HIV-1)-specific CD8 cytotoxic T-lymphocytes(CTL)are essential components of the protective immunity against HIV-1infection.However,due to heterogeneous responses of CTL to HIV-1,our general understanding of CTL efficacy in the context of HIV-1infection remains limited. To better understand the factors that determine the potency of HIV-1specific CTL responses, this study directly investigated the relationship between different functional attributes associated with CTL response at the single cell level by using HIV-1specific CTL clones isolated in vitro. Twelve selected HIV-1CTL clones with various HLA restriction and specific antigen epitopes were comprehensively evaluated by several functional assays(e.g., killing capacity, degranulation, production of multiple cytokines and polyfunctionality, as well as the expression of lytic granule components and exhaustion molecules).Our principal findings were that the killing capacity of the CTL response was most closely associated with their degranulation capacity. Additionally, the killing and the degranulation capacity of CTL was associated with the levels and polyfunctionality of the cytokines secreted later. These findings implicate that multiple functional CTL responses are coordinately regulated and determined.","PeriodicalId":8776,"journal":{"name":"Bing du xue bao = Chinese journal of virology","volume":"32 3","pages":"331-41"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36276259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Research Progress in Laboratory Diagnostic Methods for HEV Infection]. 【HEV感染实验室诊断方法研究进展】。

Bing du xue bao = Chinese journal of virology Pub Date : 2016-05-01

Wenfang Ji, Guiping Wen, Zimin Tang, Ningshao Xia, Zizheng Zheng

{"title":"[Research Progress in Laboratory Diagnostic Methods for HEV Infection].","authors":"Wenfang Ji, Guiping Wen, Zimin Tang, Ningshao Xia, Zizheng Zheng","doi":"","DOIUrl":"","url":null,"abstract":"Hepatitis E, an acute self-limited disease is caused by hepatitis E virus(HEV)and is a public-health concern for people worldwide. HEV is transmitted primarily via the fecal-oral route while direct evidence for blood-borne transmission has been reported. So the risk of blood transfusion safety caused by HEV has been widely paid attention. Here, we aim to provide some references to HEV screening for blood donors through analyzing the existing diagnostic methods for HEV, which are of great significance for the prevention and control of HEV infection. Currently, the primary detection indexes for HEV primarily include HEV RNA,HEV antigen, anti-HEV IgM, and anti-HEV IgG. HEV RNA testing is considered to be the \"gold standard\" for the detection of HEV infection. This test takes advantage of patients with chronic Hepatitis E, immunosuppressed people, and patients with nonhepatic manifestations of hepatitis E.HEV antigen testing is regarded as a current infection index for HEV, which could be used to detect HEV in blood donors and diagnose acute HEV infection. Anti-HEV IgM is a mark of HEV recent infection, but not a single index to diagnose a current infection with HEV. Anti-HEV IgG indicates that HEV was the previous infection, and it is not suitable to diagnose acute HEV infection. At present, blood donors screening for HEV were mainly based on nucleic acid detection, and an antigen test possibly could cover its’ shortage. To confirm the value of the antigen and antibody tests for blood donor screening, further studies are required in the future.","PeriodicalId":8776,"journal":{"name":"Bing du xue bao = Chinese journal of virology","volume":"32 3","pages":"385-92"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36276198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Expression,Purification,Structure Determination and Immunogenicity Assay of Hepatitis E Virus Capsid Protein p495 Derived from Baculovirus-based Insect Cell]. [基于杆状病毒的昆虫细胞中戊型肝炎病毒衣壳蛋白p495的表达、纯化、结构测定和免疫原性分析]。

Bing du xue bao = Chinese journal of virology Pub Date : 2016-05-01

Qingshan Lin, Jie Jiang, Tingting Li, Yuyun Zhang, Zhenyong Zhang, Minghua Zheng, Kaihang Wang, Qingbing Zheng, Hai Yu, Ying Gu, Ningshao Xia, Shaowei Li

{"title":"[Expression,Purification,Structure Determination and Immunogenicity Assay of Hepatitis E Virus Capsid Protein p495 Derived from Baculovirus-based Insect Cell].","authors":"Qingshan Lin, Jie Jiang, Tingting Li, Yuyun Zhang, Zhenyong Zhang, Minghua Zheng, Kaihang Wang, Qingbing Zheng, Hai Yu, Ying Gu, Ningshao Xia, Shaowei Li","doi":"","DOIUrl":"","url":null,"abstract":"Our objective was to establish a robust method for the expression and purification of hepatitis E virus(HEV)p495protein using a baculovirus-based insect cell expression system; to determine the properties and cryo-EM structure of the resulting virus-like particles(VLPs);and to compare their immunogenicity with p239 particles in the commercial hepatitis E vaccine (Hecolin). The sequence spanning HEV ORF2 amino acids 112-606 in the genotype I HEV isolate was cloned into baculovirus to express recombinant p495 protein. ELISA, analytical ultracentrifugation, size-exclusion chromatography and negative-staining transmission electron microscopy(TEM)were carried out to characterize the physicochemical properties of p495.Recombinant p495 VLPs were obtained successfully from the insect cell expression system with purity of>95%and yield of 15mg/L.The recombinant HEV p495 protein was homogeneous in solutions. The 3Dstructure of p495 VLPs was determined by cryo-EM;it was icosahedral with T=1arrangement,and showed good congruency with the crystal structure in the literature(PDB ID:2ZZQ).In mouse vaccination experiments,p495 conferred comparable immunogenicity with that of p239 antigen in Hecolin. Thus, a robust and scalable approach to obtain homogeneous, immunogenic HEV p495 VLPs has been established. This study may assist investigations of HEV receptors, epitope mapping, vaccine improvement and so on.","PeriodicalId":8776,"journal":{"name":"Bing du xue bao = Chinese journal of virology","volume":"32 3","pages":"342-8"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36276261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Preparation,Identification,and Application of Monoclonal Antibody against Orf Virus 118 Protein]. [口蹄疫病毒118蛋白单克隆抗体的制备、鉴定及应用]。

Bing du xue bao = Chinese journal of virology Pub Date : 2016-05-01

Bin Xiao, Wenbo Hao, Zhenglun Du, Xiaoqing Liao, Shuhong Luo

{"title":"[Preparation,Identification,and Application of Monoclonal Antibody against Orf Virus 118 Protein].","authors":"Bin Xiao, Wenbo Hao, Zhenglun Du, Xiaoqing Liao, Shuhong Luo","doi":"","DOIUrl":"","url":null,"abstract":"This study was designed to prepare a monoclonal antibody against Orf virus 118 protein and explore the biological properties of ORFV118 using this antibody. We constructed a recombinant plasmid pET33b-ORFV118 that contained a full-length ORFV118 gene. The plasmid was transformed into E.coli BL21,and the expression of ORFV118 was induced by isopropyl-β-d-thiogalactoside (IPTG).Prokaryotic ORFV118 was purified via Ni-NTA affinity chromatography and was subsequently used as an antigen to immunize mice. An anti-ORFV118 antibody was prepared using hybridoma technology. The titer and specificity of this antibody were tested by an indirect ELISA and Western blot/immunohistochemistry, respectively. We successfully obtained three antibody-secreting hybridomas,1A2,3B5,and 5D10.The titers of the three hybridomas were 1:10000,1:6400,and 1:8000.The monoclonal antibody (mAb),1A2 and the highest titer was selected for further research. The mAb 1A2,an IgG1 type antibody was bonded to its immunizing antigen, both the eukaryotic and natural ORFV118 with high specificity. The immunohistochemical analysis showed that the focal specific staining was restricted to the epidermal layer and subcutaneous tissue, which conformed to the characteristics of an ORFV infection. The mAb 1A2 recognized ORFV118with high specificity. Further study of mAb 1A2 will facilitate our understanding of ORFV118 and provide potentially novel methods for the diagnosis, prevention, and treatment of Orf.","PeriodicalId":8776,"journal":{"name":"Bing du xue bao = Chinese journal of virology","volume":"32 3","pages":"292-7"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36276448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Molecular Identification and Variation Analysis of the NSP2 and ORF5 Genes of PRRSV in Henan Province from 2014 to 2015]. [2014 - 2015年河南省PRRSV NSP2和ORF5基因的分子鉴定及变异分析]。

Bing du xue bao = Chinese journal of virology Pub Date : 2016-05-01

Tianzhun Guo, Hongtao Chang, Dandan Cui, Xingang Wang, Feng Zhou, Jun Zhao, Xia Yang, Yongtao Li, Lu Chen, Chuanqing Wang

{"title":"[Molecular Identification and Variation Analysis of the NSP2 and ORF5 Genes of PRRSV in Henan Province from 2014 to 2015].","authors":"Tianzhun Guo, Hongtao Chang, Dandan Cui, Xingang Wang, Feng Zhou, Jun Zhao, Xia Yang, Yongtao Li, Lu Chen, Chuanqing Wang","doi":"","DOIUrl":"","url":null,"abstract":"This study aimed at investigating the molecular epidemiology and genetic variation of PRRSV based on the detection of 250 clinical samples collected from 118 farms from 2014 to 2015in different regions of Henan Province by RT-PCR. The NSP2 and ORF5genes of the PRRSV-positive samples were sequenced and analyzed. The results showed that 58 samples were positive for PRRSV, with a positive rate of 23.2%.A total of 29NSP2 and 31ORF5genes were obtained. The phylogenetic analysis revealed that most of the prevalent strains belonged to the North American genotype. Among the 58 positive samples,14 strains were highly homologous with HP-PRRSV, another 15 samples were highly homologous with the North American prevalent strain, and NADC30 contained a discontinuous deletion of 131 amino acids in Nsp2,which had been recently reported in China and Korea. This study showed that the HP-PRRSV and NADC30-Like strains are presently the dominant strains in Henan,particularly in comparison with the results from 2012 to 2013.Moreover,the NADC30-Like strains accounted for a higher percentage. In addition, both the NSP2 and ORF5genes had significant variations, suggesting that more attention should be continuously paid to monitor the pathogenic epidemiology and genetic variation of PRRSV. Furthermore, additional research should be conducted regarding the mechanism of pathogenicity and immunological suppression of PRRSV to provide a reference for the research and development of vaccines and antiviral drugs.","PeriodicalId":8776,"journal":{"name":"Bing du xue bao = Chinese journal of virology","volume":"32 3","pages":"298-307"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36276453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The Preparation of Epitope-based Recombinant Rubella Virus Diagnostic Antigen]. 基于表位的重组风疹病毒诊断抗原的制备

Bing du xue bao = Chinese journal of virology Pub Date : 2016-05-01

Qiudong Su, Minzhuo Guo, Feng Qiu, Zhiyuan Jia, Xuexin Lu, Qingling Meng, Ruiguang Tian, Shengli Bi, Yao Yi

{"title":"[The Preparation of Epitope-based Recombinant Rubella Virus Diagnostic Antigen].","authors":"Qiudong Su, Minzhuo Guo, Feng Qiu, Zhiyuan Jia, Xuexin Lu, Qingling Meng, Ruiguang Tian, Shengli Bi, Yao Yi","doi":"","DOIUrl":"","url":null,"abstract":"To prepare an epitope-based recombinant Rubella virus (RV) recombinant diagnostic antigen(designated ‘H29’) and preliminarily evaluate its antigenicity. With Glutathione S-Transferase (GST) located at the N-terminal, and the His tag at the C-terminal, the epitope-based RV recombinant diagnostic antigen (designated‘H29’) was expressed in Escherichia coli (E.coli) and purified by affinity and anion exchange chromatography. Based on the antigenicity of H29 identified by Western blot (WB), we constructed and evaluated a novel early diagnostic ELISA for RV infection. The soluble H29 protein with a high homogeneity was obtained; the WB analysis demonstrated that the H29 protein could bind to a monoclonal antibody for RV-E1 and GST antigens, as well as detect RV acute-phase serum. Using the novel ELISA, the serum from 48 cases with positive RV infection,48 cases with negative RV infection, and 48 healthy people was detected, displaying the excellent consistency. Using prokaryotic expression and chromatography purification, the epitope-based recombinant RV-IgM diagnostic antigen was obtained with excellent antigenicity, which could be applied for the serological detection of the early infection with RV.","PeriodicalId":8776,"journal":{"name":"Bing du xue bao = Chinese journal of virology","volume":"32 3","pages":"249-55"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36274666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The Expression and Purification of P[4],P[6] and P[8] Rotavirus VP8 * core Proteins]. P[4]、P[6]、P[8]轮状病毒VP8 *核心蛋白的表达与纯化[j]。

Bing du xue bao = Chinese journal of virology Pub Date : 2016-05-01

Xiaoman Sun, Nijun Guo, Dandi Li, Xin Ma, Zhaojun Duan

引用次数: 0