[The Expression and Purification of P[4],P[6] and P[8] Rotavirus VP8 * core Proteins].

Abstract

P[4], P[6] and P[8] rotaviruses (RVs) are the most prevalent RV genotypes in the population. In order to further investigate the receptor binding and structural character of P[4], P[6] and P[8] RVs, VP8 * core proteins of the P[4], P[6] and P[8] RV strains isolated directly in the stool samples in China were expressed and purified with the GST and His-tag respectively. The GST-fusion protein was approximately 46 kDa while the His-tag proteins approximately 20 kDa. In conclusion, the recombinant plasmids of PGEX4T-1-VP8 * core and pET30a-VP8 * core were constructed and the VP8 * core proteins were successfully expressed in the soluble form by using E.coli expression system. These findings provide the basis for the futhure functional and structural studies of VP8 * proteins.