Bing du xue bao = Chinese journal of virology最新文献

Detection and Identification of the Bat Circovirus BtCV-DS13. 蝙蝠圆环病毒BtCV-DS13的检测与鉴定

Bing du xue bao = Chinese journal of virology Pub Date : 2017-01-01

Yi Wang, Gufang Shen, Dan Hu, Sitong Qian, Changqiang Zhu, Weilong Tan, Changjun Wang

引用次数: 0

Research on the Mechanism of Cross-species Infection and Transmission for Animal-origin Emerging and Re-emerging Zoonosis Has Won Support by the National Key Research and Development Program of China. 动物源性新发和再发人畜共患病跨物种感染和传播机制研究获得国家重点研发计划支持。

Bing du xue bao = Chinese journal of virology Pub Date : 2017-01-01

Weizheng Yan, Na Zhu, Baoying Huang

引用次数: 0

Comparison of Pathogenicity between PR8F and Different Mutants on the NS1 Locus in Mice. PR8F与NS1基因座不同突变体致病性的比较。

Bing du xue bao = Chinese journal of virology Pub Date : 2017-01-01

Zhuodong Cui, Dan Chai, Changzhan Xie, Shubo Wen, Ping Zhang, Yilong Zhu, Yunxia Liu, Yiquan Li, Mingyao Tian, Ningyi Jin

{"title":"Comparison of Pathogenicity between PR8F and Different Mutants on the NS1 Locus in Mice.","authors":"Zhuodong Cui, Dan Chai, Changzhan Xie, Shubo Wen, Ping Zhang, Yilong Zhu, Yunxia Liu, Yiquan Li, Mingyao Tian, Ningyi Jin","doi":"","DOIUrl":"","url":null,"abstract":"The aim of this study was to explore the influence of mutation of different non-structural (NS) 1 amino-acid residues on the pathogenicity of influenza viruses and the function of NS1 virulence-related sites on the pathogenesis of influenza viruses. We analyzed segments of the NS1 protein gene and key sites related to virulence of influenza viruses based on a literature review. Fragments of the NS1 gene were cloned from the HIN1 subtype PR8F (non-mutated) and preserved by our research team with encoding sequence site-specific mutagenesis at aa42, aa8l, and aa149. Via a reverse genetics system, we rescued the mutant strains PR8F-42, PR8F-81, and PR8F-149, which were inoculated into chick embryos and could replicate stably after five passages. Efficiency of viral replication was measured by testing hemagglutination titers. BALB/c mice were inoculated With mutated or non-mutated PR8F (10(6) TCIDO(50)/100 μl for each mouse), respectively. The typical clinical manifestations (weight change and survival) were recorded. Autopsies, as well as observations of the pathologic features and pulmonary-tissue slices of mice that died after inoculation, were done. RNA of mouse lungs was extracted, and the residual quantity of virus in lungs was detected by quantitative polymerase chain reaction (qPCR). Results showed that mutation of NS1 at aa42 from Ser to Pro did not change the pathogenicity of PR8F in mice, but a low pathogenicity of PR8F occurred after mutation of NS1 at aa8l and aa149. The present study lays the foundation for further investigations of the function of NS1 pathogenicity-related sites in the pathogenesis of influenza viruses.","PeriodicalId":8776,"journal":{"name":"Bing du xue bao = Chinese journal of virology","volume":"33 1","pages":"6-12"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36905070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genotypes of the Hepatitis C Virus in Infected Patients in Hohhot, China. 呼和浩特市丙型肝炎病毒感染患者基因型分析

Bing du xue bao = Chinese journal of virology Pub Date : 2017-01-01

Junying Lang, Peng Sun, Sha Lu, Pengwei Zhao, Chunmei Wang, Caisheng Wang, Bin Li, Xiaoling Shen

{"title":"Genotypes of the Hepatitis C Virus in Infected Patients in Hohhot, China.","authors":"Junying Lang, Peng Sun, Sha Lu, Pengwei Zhao, Chunmei Wang, Caisheng Wang, Bin Li, Xiaoling Shen","doi":"","DOIUrl":"","url":null,"abstract":"We wished to identify the existing HCV genotypes in diagnosed cases of infection by the hepatitis C virus in Hohhot (China) to offer basic data for the treatment and prevention of HCV infection in this area. Outpatients and inpatients were recruited from hospitals in Hohhot from January 2014 to January 2015. In total, 149 patients with HCV infection confirmed by positive anti-HCV and HCV-RNA tests were selected. First, we extracted HCV RNA. cDNA. was obtained. using reverse transcription, then NS5B regions were amplified using a nested polymerase chain reaction (PCR), which enabled 94 cases to be sequenced. We compared sequences in NCBI BLAST, which revealed the reference sequence of maximum similarity and enabled identification of HCV genotypes. Then, a homologous relationship tree was created using MegAlign clustal W. Finally, the distribution characteristics in HCV genotypes, as well as the relationship between genotypes and host age and sex, were obtained. Genotype lb accounted for 73. 40% (69/94), 2a for 19. 14% (18/94), 3a, 3b, and 6a for 2. 12% (2/94), respectively, and 6u for 1. 06% (1/94). The genotype distribution of men and women showed no significant difference (P>O . 05) in 93 cases for whoni explicit sex-specific data were available. Age data for 90 patients revealed the age of disease onset of the 1and2 types to be significantly higher than that of the 3and6 -types (P<0. 05). Hence, genotype lb was the most prevalent in Hohhot (lb has been reported to be the -predominant genotype in the general population of China, followed by genotype 2a). Other, less prevalent genotypes were 3a, 3b, 6a and 6u. Genotype 4 and genotype 5 were not found.","PeriodicalId":8776,"journal":{"name":"Bing du xue bao = Chinese journal of virology","volume":"33 1","pages":"61-66"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36905074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis on Genotype Distribution and Mutation of Major Hydroponic Region of Hepatitis B Virus in Henan Province. 河南省乙型肝炎病毒主要水培区基因型分布及突变分析。

Bing du xue bao = Chinese journal of virology Pub Date : 2017-01-01

Yonghao Guo, Daxing Feng, Pumei Dong, Shuang Zhang, Jin Xu, Yanyang Zhang, Zhe Wang

引用次数: 0

KNK437 Inhibits Replication and Transcription of the Hepatitis B Virus. KNK437抑制乙型肝炎病毒复制和转录

Bing du xue bao = Chinese journal of virology Pub Date : 2017-01-01

Kanghong Hu, Yayun Huang, Jingfang Mu, Zhikui Cheng, Xiang Zhu

{"title":"KNK437 Inhibits Replication and Transcription of the Hepatitis B Virus.","authors":"Kanghong Hu, Yayun Huang, Jingfang Mu, Zhikui Cheng, Xiang Zhu","doi":"","DOIUrl":"","url":null,"abstract":"During replication of the hepatitis B virus (HBV) in liver cells, the reverse transcription of pre-genomic RNA (pgRNA) is initiated by protein priming at an RNA packaging signal ε located near the 5' end of pgRNA. Heat-shock proteins (Hsps) such as Hsc70, Hsp40, and Hsp90 have been reported to be involved in the reconstitution of HBV polymerase (P protein) and E. The P - E complex initiates the reverse transcription and assembly of nucleocapsids. Hence, blockade of P - ε interactions is an attractive target for drug intervention. We explored the influence of the Hsp inhibitor KNK437 on replication and transcription of the HBV. Three working models were applied: HepG2. 2. 15 cell line; Huh7 cells transfected transiently with the 1. 05 X HBV (pCH9-3091) plasmid; Huh7 cells transfected transiently with the 1. 3 X HBV (pGEM-1. 3 X HBV) plasmid. Cytotoxic effects of KNK437 were detected by the CCK-8 method. Levels of hepatitis B surface antigen (HBsAg) and hepatitis B viral protein (HBeAg) in the media secreted from cells were measured using an ELISA. Intracellular HBV DNAs within nucleocapsids were measured by quantitative polymerase chain reaction (qPCR), and intracellular HBV RNAs by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Transcription of Hsps in cells was determined by qRT-PCR. Data suggested that KNK437 reduced the extracellular secretion of HBsAg and HBeAg in most cases; it downregulated expression of intracellular HBV DNAs within nucleocapsids and RNA transcripts. The lowest rate of viral DNAs in KNK437-treated hepatocytes for all experimental groups was ~1. 5%o (control, 100%), whereas that for RNAs was ~30%. Western blotting revealed KNK437 to inhibit intracellular core expression in HepG2. 2. 15. As a general inhibitor, KNK437 suppressed transcription of hsp70, hsp90b, and hsp4o. These data suggest that KNK437 may be a potent anti-HBV inhibitor for future therapy against chronic hepatitis.","PeriodicalId":8776,"journal":{"name":"Bing du xue bao = Chinese journal of virology","volume":"33 1","pages":"24-35"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36915788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Detection of HCV Genotypes 1b and 2a in China. 反转录环介导的等温扩增法检测中国HCV基因型1b和2a。

Bing du xue bao = Chinese journal of virology Pub Date : 2017-01-01

Na Zhao, Jinxia Liu, Dianxing Sun

{"title":"A Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Detection of HCV Genotypes 1b and 2a in China.","authors":"Na Zhao, Jinxia Liu, Dianxing Sun","doi":"","DOIUrl":"","url":null,"abstract":"Reverse transcription loop-mediated isothermal amplification (RT-LAMP) was used to detect hepatitis C virus (HCV) lb and 2a (the major genotypes in China). First, clinical samples were collected, and the genotypes and viral load determined. Second, RT-LAMP primers of HCV lb and 2a were designed according to the 5' untranslated region of the HCV. Third, the specificity of RT-LAMP was tested by non-lb and non-2a HCV viruses, and the products analyzed by endonuclease. Fourth, the limit of detection was tested using diluted samples, and the results determined by calcein/Mn(2+) -dependent visual methods. Finally, the consistency of RT-LAMP and real-time fluorescent polymerase chain reaction (PCR) was compared to evaluate the clinical practicality. Results showed that RT-LAMP primers had good specificity because there was no cross-reactivity and the target sequences were identified correctly by endonuclease. The limit of detection was 100 IU/mL and calcein/Mn(Z+)-dependent visual methods were easier to use compared with electrophoresis. After analyses of all amplification results by statistical software; we found no significant difference between RT-LAMP and real-time fluorescent PCR (P> 0. 05). In conclusion, RT-LAMP requires only simple apparatus and simple operation, which is very helpful in primary-health-care institutions.","PeriodicalId":8776,"journal":{"name":"Bing du xue bao = Chinese journal of virology","volume":"33 1","pages":"49-55"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36905072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Infectious Salmon Anaemia Virus from Imported Iced Atlantic Salmons. 进口大西洋冰鲑鱼传染性鲑鱼贫血病毒的鉴定。

Bing du xue bao = Chinese journal of virology Pub Date : 2017-01-01

Ying Liu, Peng Jia, Jinjin Wang, Xiujie Shi, Li Yu, Xiaocong Zheng, Junqiang He, Wensheng Lan, Hong Liu

引用次数: 0

Droplet Digital Polymerase Chain Reaction Method for Absolute Quantification of Influenza A Viruses. 液滴数字聚合酶链反应法绝对定量甲型流感病毒。

Bing du xue bao = Chinese journal of virology Pub Date : 2017-01-01

Zhaomin Feng, Xiang Zhao, Xiaohui Zou, Wenfei Zhu, Yongkun Chen, Dayan Wang, Yuelong Shu

引用次数: 0

How the Canine Distemper Virus Infects Human Cells at the Molecular Level in Vitro. 犬瘟热病毒如何在体外分子水平上感染人类细胞。

Bing du xue bao = Chinese journal of virology Pub Date : 2017-01-01

Xuefeng Cao, Yinan Tian, Xiangming Huang, Wei Qiu, Yanyi Yang, Chao Gong, Kuixing Yang, Bo Yuan, Guangneng Peng, Zhijun Zhong

引用次数: 0