Detection and Identification of the Bat Circovirus BtCV-DS13.

Abstract

The bat circovirus has been detected from different bat species and regions after detection in Rousettus leschenaultia. To enrich the epidemiologic data of the bat circovirus, a complete sequence named "BtCV-DS13" was obtained by nested polymerase chain reaction and Genome Walking? based on the intestines of Myotis davidii from Zhoushan Island (Zhejiang Province, China). The complete length of BtCV-DS13 was 1873nt, and it had the typical gene structure of a circovirus according to sequencing analyses. The nucleotide sequence identity was 22. 9%, 53. 5%, 24. 7% and 4. 5% for bat 1, bat 2, bat 3, and a bat infected with the cyclovirus, respectively. Phylogenetic analyses based on the full-length sequence strongly supports the suggestion that BtCV-DS13, the bat circovirus, and porcine circovirus should be clustered into the genus Circovirus. These results imply that BtCV-DS13 should be a new bat circovirus.