BioChip Journal最新文献

{"title":"Functionalized Carbon Quantum Dots Derived from Zelkova serrata Plant Leaves for the Detection of Normetanephrine in Geriatric Plasma Samples and ROS-Induced Antibacterial Applications Using a Plausible Mechanistic Approach","authors":"Yanamala Poojitha, Gayathri Chellasamy, Saravanan Govindaraju, Kyusik Yun","doi":"10.1007/s13206-023-00136-w","DOIUrl":"https://doi.org/10.1007/s13206-023-00136-w","url":null,"abstract":"<p>Functionalized carbon quantum dots with tunable optical properties widely used in sensor applications. In this study, carbon quantum dots were synthesized from <i>Zelkova serrata</i> leaves (ZCQDs) in an aqueous medium via a single-step hydrothermal reaction with essential reactive functional groups. As-synthesized ZCQDs (average size, 3–7 nm) were characterized and confirmed to contain hydroxyl/amine and carboxylic acid functional groups. Photoluminescence spectral analysis revealed that the fluorescence intensity of ZCQDs drastically decreased after the addition of different concentrations of normetanephrine (NM) in deionized water and geriatric plasma samples. Our developed strategy could detect NM with a limit of detection of 7.96 and 86.2 nM in deionized water and geriatric plasma samples, respectively. Interestingly, Fourier transform infrared spectroscopy revealed a new peak at 1660 cm⁻¹, confirming the formation of the keto (C=O) group in NM. In addition, significant peak shifts were observed in the C 1s, and O 1s deconvoluted X-ray photoelectron spectra. Bandgap calculations also revealed significant interactions between NM and ZCQDs. Antibacterial activities of ZCQDs were investigated in <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>, and potent activities were observed in <i>Staphylococcus aureus</i> at a half-maximal inhibitory concentration of 32 μg/mL via the generation of intracellular reactive oxygen species. By enabling specific therapies and improving our understanding of intricate biological processes at the nanoscale, these materials have the potential to completely transform the biomedical field. Our findings suggest the involvement of a working mechanism in transferring electrons between the conductance band of ZCQDs and the acidic protons of N, to produce the oxidized form NM for photoluminescence quenching of ZCQDs.</p>","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"4 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139768755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in Technical Assessment of Spiral Inertial Microfluidic Devices Toward Bioparticle Separation and Profiling: A Critical Review","authors":"Mahsa Bagi, Fatemeh Amjad, Seyed Majid Ghoreishian, Somayeh Sohrabi Shahsavari, Yun Suk Huh, Mostafa Keshavarz Moraveji, Sirivatch Shimpalee","doi":"10.1007/s13206-023-00131-1","DOIUrl":"https://doi.org/10.1007/s13206-023-00131-1","url":null,"abstract":"<p>Separation of micro- and nano-sized bioparticles is essential for efficient diagnostics, chemical and biological analyses, drug development, food and chemical processing, and environmental monitoring. However, most of the currently available bio-separation techniques are based on the membrane filtration approach, whose efficiency is restricted by membrane-related disadvantages, including pore size, surface charge density, and biocompatibility, which results in a reduction in the isolation resolution. To address these issues, till now, many microfluidic devices have been developed for particle/cell profiling due to their excellent sensitivity and specificity, less sample consumption, shortened processing time, and high throughput features. Of the various microfluidic systems, the spiral inertial microfluidic technique has recently attracted attention as an innovative strategy and advanced cutting-edge technology toward bioparticle separation. Depending on the needs of the microfluidic device, the spiral inertial chip can be customized to separate bioparticles owing to their sizes and different shapes. In this review, we discuss the kinematics of microchannel particle separation mechanisms, recent developments in the inertial microfluidic device realm, and their applications for the separation of several types of bioparticles, including blood cells, stem cells, sperm cells, pathogens, and algae. Finally, we highlight challenges and economical perspectives associated with guidelines for further development of spiral inertial microfluidic devices in the future.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>\u0000","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"10 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139517080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intensity Histogram-Based Reliable Image Analysis Method for Bead-Based Fluorescence Immunoassay","authors":"Sanghyun Lee, Juyeong Kim, Pankee Bae, Sangmin Lee, Hojin Kim","doi":"10.1007/s13206-023-00137-9","DOIUrl":"https://doi.org/10.1007/s13206-023-00137-9","url":null,"abstract":"<p>Bead-based fluorescence immunoassay is drawing attention as a next-generation technology in disease diagnosis owing to its high sensitivity and multiplexing capability. Fluorescence imaging of beads is typically used to determine their mean fluorescence intensity. However, the mean intensity can be evaluated differently depending on the analysis methods [such as the shape and size of the region of interest (ROI)]. To address these problems, this study proposes a highly reliable and reproducible image analysis method utilizing a fluorescence intensity-based effective pixel extraction technique. Various potential sources of defective signals (e.g., fluorescence aggregation, non-specific antigen–antibody reactions, and bead defects) can be prevented from contributing to the average value by selectively extracting pixels representing the specific reactions of antigens and antibodies in the ROI. In this study, we fabricated a microfluidic chip composed of multiple bead-based detection lines, performed fluorescence immunoassay, and then compared the mean fluorescence intensity calculated from the fluorescence images with that of a conventional analysis method. Using the conventional method, the evaluated average mean intensity value of beads varied significantly based on the size of the ROI with the coefficients of variation ranging from approximately 29–95%. In contrast, the effective pixel extraction method resulted in a coefficient of variation of approximately 3–7% under varying ROI size. Furthermore, the coefficients of variation for four detection lines containing various types of defective signals significantly decreased from approximately 7.1% to 2.6%. The proposed technique will help in minimizing the analysis deviation caused by different ROI selections or defective signals in fluorescent image-based immunoassays.</p>","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"3 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139470303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Numerical-Simulation-Based Buffer Design for Microchip Electrophoresis with Capacitively Coupled Contactless Conductivity Detection","authors":"","doi":"10.1007/s13206-023-00135-x","DOIUrl":"https://doi.org/10.1007/s13206-023-00135-x","url":null,"abstract":"<h3>Abstract</h3> <p>We present a numerical simulation method for designing a buffer system in microchip electrophoresis (MCE) equipped with capacitively-coupled-contactless-conductivity detection (C4D). One of the key design considerations for MCE-C4D is background electrolyte (BGE). This is because a C4D typically exhibits low sensitivity, and optimizing BGE conditions (e.g., base and acid species, pH, and ionic strength) can improve its sensitivity. However, BGE has been traditionally designed through experience or trial and error, which is time- and reagent-consuming. In this study, we employ Simul 5, an open-source electrophoresis simulation software, for rational BGE design. Four BGEs including trimethylamine (TEA)/acetic acid (AcOH, pH 10.6), MES/His (pH 6.1), MES/TRIS (pH 8.1), and TRIS/HCl (pH 7.4), previously used in electrophoresis-C4D of amino acids and protein, were selected for evaluation of our numerical method. Glutamic acid (Glu) was selected as a model analyte for initial simulation verification. Our numerical simulation revealed that the best achievable detection sensitivity was 1.046 × 10^–5 S/(m µM) in the TRIS/HCl buffer because anionic Glu species with a low mobility (27 × 10^–9 m²/Vs) replaced Cl⁻ co-ion of a high mobility (79.1 × 10^–9 m²/Vs) in the analyte zone, leading to a significant negative conductivity peak. TEA/AcOH, MES/His, and MES/TRIS buffers exhibited progressively lower sensitivity. After the initial evaluation, trypsin inhibitor (TI), a more complex proteinous analyte was tested in the MES/His and MES/TRIS BGEs. The best detection sensitivity was 1.032 × 10^–4 S/(m µM) in the MES/TRIS buffer because counter-ionic species TRIS⁺ of a high mobility (29.5 × 10^–9 m²/Vs) was replaced by the ionic TI, characterized by a large charge (− 11.5) and a low mobility (8.08 × 10^–9 m²/Vs), resulting in a strong negative peak. Based on a comprehensive analysis of the impacts of compositional changes in each ionic species of the analyte zone on conductivity-peak height, we propose a BGE design guideline for enhanced sensitivity. Subsequent MCE-C4D confirmation experiments demonstrated excellent qualitative agreement with the simulation results for the Glu and TI analytes. We anticipate that our numerical analysis method will find wide application in designing BGEs for portable MCE-C4D systems by enhancing sensitivity.</p>","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"8 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139093258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Label-Free and Real-Time Electrical Impedance Monitoring of Macrophage Polarization of THP-1 Monocytes on Indium Tin Oxide Electrode","authors":"Duc-Trung Pham, Hoang Lan Pham, Hien T. Ngoc Le, My-Van Tieu, Daesoo Kim, Jae Young Kim, Sungbo Cho","doi":"10.1007/s13206-023-00132-0","DOIUrl":"https://doi.org/10.1007/s13206-023-00132-0","url":null,"abstract":"<p>Macrophages are immune cells that play important roles in the human body’s initial immune responses against pathogens and tumor cells. We investigated the use of electrical impedance monitoring to assess the differentiation of THP-1 monocyte into macrophages, which is necessary for immunotherapy research conducted. The change in resistance at 1 kHz and capacitance at 100 kHz measured were proportionally increased according to not only the increase in the density of resting macrophages differentiated by Phorbol-12-myristate-13-acetate treatment but also the initial number of THP-1 cells seeded on the electrode. Additionally, real-time impedance data from THP-1 cells after 48 h of cultivation demonstrated greater recognition of the resting macrophage phenotypes (adhesion cells) covered microelectrode surface with a significant increase of impedance signal in comparison with monocytes phenotypes (suspended cells). Furthermore, during the polarization phase of macrophages, the alternatively activated macrophage phenotype was larger and flatter than that of classically activated macrophage and resting macrophage phenotypes, indicating a correlation with a higher resistance and lower capacitance impedances at 1 kHz and 100 kHz of alternatively activated macrophages (4750 Ω and – 3.5 nF) than that of classically activated macrophages (2000 Ω and – 1.5 nF) and resting macrophages (3500 Ω and – 2.0 nF), respectively. The study’s findings demonstrated that the impedance measurement system is high sensitivity and confidence in monitoring macrophages differentiation and polarization. The electrical impedance, which has significance for each macrophage phenotype, is compatible with macrophages characteristic features observed using flow cytometry and a microscope.</p>","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"11 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139053523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Towards Nanomaterial-Incorporated Soft Actuators: from Inorganic/Organic Material-Based Soft Robot to Biomaterial-Based Biohybrid Robot","authors":"Minkyu Shin, Seewoo Kim, Ambrose Ashwin Melvin, Jeong-Woo Choi","doi":"10.1007/s13206-023-00134-y","DOIUrl":"https://doi.org/10.1007/s13206-023-00134-y","url":null,"abstract":"<p>Soft actuators have played an indispensable part in the field of biosensors and soft robotics as such systems offer solutions that cannot be addressed with rigid actuators due to the lack of both flexibility and sensitivity. However, soft actuators have certain limitations when it comes to their durability and longevity. In recent years, quite a few versatile fabrication techniques and innovative solutions have been developed that have played an essential role in the development of soft robotics. An exemplary innovation involves the integration of nanomaterials into polymers that act as a host in the fabrication of inorganic/organic actuators. These actuators have shown significant enhancement both in their physical and chemical properties. Consequently, it paves the way for the development of sophisticated soft actuator-based devices that can find broader applications in the field of biomedical sciences. However, biocompatibility has been a matter of concern for inorganic/organic soft actuators. Addressing this issue, studies on the development of biomaterial-based soft actuators that incorporate nanomaterials have been conducted for biohybrid robots. This review aims to provide a comprehensive understanding of diverse stimulus-trigger actuation alongside exploring the influence of nanomaterials in inorganic/organic actuators. Further, it gives valuable insights into the implication of biomaterials in soft actuators for the development of biohybrid robot.</p>","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"18 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139065956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of Truncated DNA Aptamer and Its Application to an Electrochemical Biosensor Consisting of an Aptamer and a MXene Heterolayer for Yellow Fever Virus","authors":"Nayeon Kwon, Siyun Lee, Moonbong Jang, Jin-Ho Lee, Chulhwan Park, Taek Lee","doi":"10.1007/s13206-023-00133-z","DOIUrl":"https://doi.org/10.1007/s13206-023-00133-z","url":null,"abstract":"<p>Yellow fever virus (YFV) is an acute infectious virus with high morbidity and mortality risks during the toxic phase. Early diagnosis and suppression are essential because YFV has no precise treatment. With the aim of detecting YFV, we fabricated a highly sensitive electrochemical biosensor comprised with a truncated DNA aptamer/MXene heterolayer. The synthesized DNA aptamer was prepared by systematic evolution of ligands using the exponential enrichment (SELEX) technique, which can specifically detect the YFV NS1 protein. MXenes increase the electrical sensitivity and the possibility of attachment of aptamers by widening the surface area. The aptamer-cutting process which called a truncation process can reduce the production cost of biosensors. The biosensor performance was evaluated using electrochemical methods, cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The limit of detection (LOD) was 2.757 pM for YFV diluted in phosphate-buffered saline (PBS) and 2.366 pM for YFV diluted in 10% human serum, proving that the biosensor specifically binds to YFV through selectivity evaluation. This biosensor can be a valuable tool for the early diagnosis of YFV, enabling timely intervention as well as facilitating the control and prevention of yellow fever outbreaks.</p>","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"6 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138717220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colorimetric Detection of Staphylococcus aureus Based on Direct Loop-Mediated Isothermal Amplification in Combination with Lateral Flow Assay","authors":"Seungjin Lee, Seung Hyeon Reo, Seokjoon Kim, Seokhwan Kim, Eun Sung Lee, Byung Seok Cha, Jiye Shin, Jinjoo Han, So Min Ahn, Han-Seung Shin, Ki Soo Park","doi":"10.1007/s13206-023-00130-2","DOIUrl":"https://doi.org/10.1007/s13206-023-00130-2","url":null,"abstract":"<p>In this study, <i>Staphylococcus aureus</i> (<i>S. aureus</i>) was detected using a system that combined direct loop-mediated isothermal amplification (LAMP) and lateral flow assays (LFA). This technology relies on sequence-specific hybridization in LFA; furthermore, it has high specificity and addresses the limitations associated with nonspecific amplification in general colorimetric LAMP. In addition, a direct boiling method was adopted to streamline DNA extraction and enable simple detection. The established technology was used to successfully detect <i>S. aureus</i> at a concentration as low as 10² colony-forming unit/mL, without cross-reactivity with other strains. The practical applicability of this technology was demonstrated by analyzing real samples such as beef jerky, cabbage, and eggshell, which were artificially spiked with <i>S. aureus</i>. This developed system may be beneficial with regard to operational simplicity, short analysis time, and high detection performance, which would enable its application in point-of-care settings and as a novel platform for detecting various pathogens.</p>","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"12 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138632389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasmonic-Multichromatic Sensor for Gallic Acid Assay Utilizing Ag-Coated Au Nanobipyramids","authors":"Mohamed H. Mostafa, Samy M. Shaban, Eslam Hafez, Jihoon Shin, Dong-Hwan Kim","doi":"10.1007/s13206-023-00129-9","DOIUrl":"https://doi.org/10.1007/s13206-023-00129-9","url":null,"abstract":"<p>Gallic acid (GA) is known for its valuable properties as an antioxidant, anti-cancer, and anti-mutagenic compound, making its detection in foods and drugs of paramount importance. In this research, we introduce a novel multichromatic sensor for GA detection, which employs the controlled growth of silver nanoparticles (AgNPs) on the surface of gold nanobipyramids (AuNBPs). The sensor exploits GA's capability to reduce AgNO₃, resulting in the growth of AgNPs and a subsequent blue shift of localized surface plasmon resonance (LSPR) as large as 195 nm. This unique multicolor response ranges from light gray to green, blue–violet, and pink, allowing for distinctive visual identification of GA concentrations. The sensor's performance demonstrates a wide dynamic range of 0–175 μM, and a detection limit (LOD) as low as 0.139 μM. Notably, the applicability of this multichromic plasmonic probe was successfully tested for GA assay in both Black and Green tea, showcasing highly satisfactory recovery efficiencies and affirming its potential for food quality control applications. The presented multichromatic sensor offers a promising approach for rapid and sensitive GA detection in various food and pharmaceutical products, enhancing the monitoring and assessment of GA content for improved product quality and safety.</p>","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"15 1-2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138514066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining Ultrasound-Mediated Intracellular Delivery with Microfluidics in Various Applications","authors":"Guangyong Huang, Lin Lin, Shixiong Wu, Haojie Dang, Xuesong Cheng, Ying Liu, Hui You","doi":"10.1007/s13206-023-00128-w","DOIUrl":"https://doi.org/10.1007/s13206-023-00128-w","url":null,"abstract":"<p>Ultrasound-mediated intracellular delivery is one of the popular technologies based on membrane rupture at present. To date, ultrasound directly acts on a large number of cells to achieve cargo delivery and has been widely used in drug delivery, disease therapy and other fields. However, the existing macroscopic methods can no longer meet the requirements of accurate tracking and analysis and are prone to extensive cell damage and even death. With the rapid advancements in microfluidic technologies, the combination of ultrasound and microfluidics (CUM) technology can effectively improve the delivery efficiency and cell survival rates. This new technology has rapidly become a new direction and focus of research. Thus, we analysed the mechanism of sonoporation and the effect of acoustic waves in a microfluidic channel. In addition, we reviewed the application of these new technologies in terms of structure and fabrication of ultrasound transducers and microfluidic devices. As regards our main objective, we hope to help researchers better understand the future developments and the challenges of new technologies. With this review, researchers can promote the development of new technologies to solve the current challenges of intracellular delivery and advance clinical applications.</p>","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"37 1-10","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138514076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}