BioChip Journal最新文献_第5页

Towards Nanomaterial-Incorporated Soft Actuators: from Inorganic/Organic Material-Based Soft Robot to Biomaterial-Based Biohybrid Robot 实现纳米材料集成软执行器：从基于无机/有机材料的软机器人到基于生物材料的生物混合机器人

IF 4.3 3区工程技术

BioChip Journal Pub Date : 2023-12-27 DOI: 10.1007/s13206-023-00134-y

Minkyu Shin, Seewoo Kim, Ambrose Ashwin Melvin, Jeong-Woo Choi

{"title":"Towards Nanomaterial-Incorporated Soft Actuators: from Inorganic/Organic Material-Based Soft Robot to Biomaterial-Based Biohybrid Robot","authors":"Minkyu Shin, Seewoo Kim, Ambrose Ashwin Melvin, Jeong-Woo Choi","doi":"10.1007/s13206-023-00134-y","DOIUrl":"https://doi.org/10.1007/s13206-023-00134-y","url":null,"abstract":"Soft actuators have played an indispensable part in the field of biosensors and soft robotics as such systems offer solutions that cannot be addressed with rigid actuators due to the lack of both flexibility and sensitivity. However, soft actuators have certain limitations when it comes to their durability and longevity. In recent years, quite a few versatile fabrication techniques and innovative solutions have been developed that have played an essential role in the development of soft robotics. An exemplary innovation involves the integration of nanomaterials into polymers that act as a host in the fabrication of inorganic/organic actuators. These actuators have shown significant enhancement both in their physical and chemical properties. Consequently, it paves the way for the development of sophisticated soft actuator-based devices that can find broader applications in the field of biomedical sciences. However, biocompatibility has been a matter of concern for inorganic/organic soft actuators. Addressing this issue, studies on the development of biomaterial-based soft actuators that incorporate nanomaterials have been conducted for biohybrid robots. This review aims to provide a comprehensive understanding of diverse stimulus-trigger actuation alongside exploring the influence of nanomaterials in inorganic/organic actuators. Further, it gives valuable insights into the implication of biomaterials in soft actuators for the development of biohybrid robot.","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"18 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139065956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of Truncated DNA Aptamer and Its Application to an Electrochemical Biosensor Consisting of an Aptamer and a MXene Heterolayer for Yellow Fever Virus 截短 DNA 合聚体的合成及其在由合聚体和 MXene 异质层组成的黄热病病毒电化学生物传感器中的应用

IF 4.3 3区工程技术

BioChip Journal Pub Date : 2023-12-18 DOI: 10.1007/s13206-023-00133-z

Nayeon Kwon, Siyun Lee, Moonbong Jang, Jin-Ho Lee, Chulhwan Park, Taek Lee

{"title":"Synthesis of Truncated DNA Aptamer and Its Application to an Electrochemical Biosensor Consisting of an Aptamer and a MXene Heterolayer for Yellow Fever Virus","authors":"Nayeon Kwon, Siyun Lee, Moonbong Jang, Jin-Ho Lee, Chulhwan Park, Taek Lee","doi":"10.1007/s13206-023-00133-z","DOIUrl":"https://doi.org/10.1007/s13206-023-00133-z","url":null,"abstract":"Yellow fever virus (YFV) is an acute infectious virus with high morbidity and mortality risks during the toxic phase. Early diagnosis and suppression are essential because YFV has no precise treatment. With the aim of detecting YFV, we fabricated a highly sensitive electrochemical biosensor comprised with a truncated DNA aptamer/MXene heterolayer. The synthesized DNA aptamer was prepared by systematic evolution of ligands using the exponential enrichment (SELEX) technique, which can specifically detect the YFV NS1 protein. MXenes increase the electrical sensitivity and the possibility of attachment of aptamers by widening the surface area. The aptamer-cutting process which called a truncation process can reduce the production cost of biosensors. The biosensor performance was evaluated using electrochemical methods, cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The limit of detection (LOD) was 2.757 pM for YFV diluted in phosphate-buffered saline (PBS) and 2.366 pM for YFV diluted in 10% human serum, proving that the biosensor specifically binds to YFV through selectivity evaluation. This biosensor can be a valuable tool for the early diagnosis of YFV, enabling timely intervention as well as facilitating the control and prevention of yellow fever outbreaks.","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"6 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138717220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Colorimetric Detection of Staphylococcus aureus Based on Direct Loop-Mediated Isothermal Amplification in Combination with Lateral Flow Assay 基于直接环路介导等温扩增与侧流检测相结合的金黄色葡萄球菌比色检测法

IF 4.3 3区工程技术

BioChip Journal Pub Date : 2023-12-14 DOI: 10.1007/s13206-023-00130-2

Seungjin Lee, Seung Hyeon Reo, Seokjoon Kim, Seokhwan Kim, Eun Sung Lee, Byung Seok Cha, Jiye Shin, Jinjoo Han, So Min Ahn, Han-Seung Shin, Ki Soo Park

{"title":"Colorimetric Detection of Staphylococcus aureus Based on Direct Loop-Mediated Isothermal Amplification in Combination with Lateral Flow Assay","authors":"Seungjin Lee, Seung Hyeon Reo, Seokjoon Kim, Seokhwan Kim, Eun Sung Lee, Byung Seok Cha, Jiye Shin, Jinjoo Han, So Min Ahn, Han-Seung Shin, Ki Soo Park","doi":"10.1007/s13206-023-00130-2","DOIUrl":"https://doi.org/10.1007/s13206-023-00130-2","url":null,"abstract":"In this study, Staphylococcus aureus (S. aureus) was detected using a system that combined direct loop-mediated isothermal amplification (LAMP) and lateral flow assays (LFA). This technology relies on sequence-specific hybridization in LFA; furthermore, it has high specificity and addresses the limitations associated with nonspecific amplification in general colorimetric LAMP. In addition, a direct boiling method was adopted to streamline DNA extraction and enable simple detection. The established technology was used to successfully detect S. aureus at a concentration as low as 102 colony-forming unit/mL, without cross-reactivity with other strains. The practical applicability of this technology was demonstrated by analyzing real samples such as beef jerky, cabbage, and eggshell, which were artificially spiked with S. aureus. This developed system may be beneficial with regard to operational simplicity, short analysis time, and high detection performance, which would enable its application in point-of-care settings and as a novel platform for detecting various pathogens.","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"12 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138632389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasmonic-Multichromatic Sensor for Gallic Acid Assay Utilizing Ag-Coated Au Nanobipyramids 利用银包覆金纳米金字塔检测没食子酸的等离子体多色传感器

IF 4.3 3区工程技术

BioChip Journal Pub Date : 2023-12-06 DOI: 10.1007/s13206-023-00129-9

Mohamed H. Mostafa, Samy M. Shaban, Eslam Hafez, Jihoon Shin, Dong-Hwan Kim

{"title":"Plasmonic-Multichromatic Sensor for Gallic Acid Assay Utilizing Ag-Coated Au Nanobipyramids","authors":"Mohamed H. Mostafa, Samy M. Shaban, Eslam Hafez, Jihoon Shin, Dong-Hwan Kim","doi":"10.1007/s13206-023-00129-9","DOIUrl":"https://doi.org/10.1007/s13206-023-00129-9","url":null,"abstract":"Gallic acid (GA) is known for its valuable properties as an antioxidant, anti-cancer, and anti-mutagenic compound, making its detection in foods and drugs of paramount importance. In this research, we introduce a novel multichromatic sensor for GA detection, which employs the controlled growth of silver nanoparticles (AgNPs) on the surface of gold nanobipyramids (AuNBPs). The sensor exploits GA's capability to reduce AgNO3, resulting in the growth of AgNPs and a subsequent blue shift of localized surface plasmon resonance (LSPR) as large as 195 nm. This unique multicolor response ranges from light gray to green, blue–violet, and pink, allowing for distinctive visual identification of GA concentrations. The sensor's performance demonstrates a wide dynamic range of 0–175 μM, and a detection limit (LOD) as low as 0.139 μM. Notably, the applicability of this multichromic plasmonic probe was successfully tested for GA assay in both Black and Green tea, showcasing highly satisfactory recovery efficiencies and affirming its potential for food quality control applications. The presented multichromatic sensor offers a promising approach for rapid and sensitive GA detection in various food and pharmaceutical products, enhancing the monitoring and assessment of GA content for improved product quality and safety.","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"15 1-2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138514066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combining Ultrasound-Mediated Intracellular Delivery with Microfluidics in Various Applications 超声介导的细胞内递送与微流体在各种应用中的结合

IF 4.3 3区工程技术

BioChip Journal Pub Date : 2023-11-28 DOI: 10.1007/s13206-023-00128-w

Guangyong Huang, Lin Lin, Shixiong Wu, Haojie Dang, Xuesong Cheng, Ying Liu, Hui You

{"title":"Combining Ultrasound-Mediated Intracellular Delivery with Microfluidics in Various Applications","authors":"Guangyong Huang, Lin Lin, Shixiong Wu, Haojie Dang, Xuesong Cheng, Ying Liu, Hui You","doi":"10.1007/s13206-023-00128-w","DOIUrl":"https://doi.org/10.1007/s13206-023-00128-w","url":null,"abstract":"Ultrasound-mediated intracellular delivery is one of the popular technologies based on membrane rupture at present. To date, ultrasound directly acts on a large number of cells to achieve cargo delivery and has been widely used in drug delivery, disease therapy and other fields. However, the existing macroscopic methods can no longer meet the requirements of accurate tracking and analysis and are prone to extensive cell damage and even death. With the rapid advancements in microfluidic technologies, the combination of ultrasound and microfluidics (CUM) technology can effectively improve the delivery efficiency and cell survival rates. This new technology has rapidly become a new direction and focus of research. Thus, we analysed the mechanism of sonoporation and the effect of acoustic waves in a microfluidic channel. In addition, we reviewed the application of these new technologies in terms of structure and fabrication of ultrasound transducers and microfluidic devices. As regards our main objective, we hope to help researchers better understand the future developments and the challenges of new technologies. With this review, researchers can promote the development of new technologies to solve the current challenges of intracellular delivery and advance clinical applications.","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"37 1-10","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138514076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Progress in Rapid Biosensor Fabrication Methods: Focus on Electrical Potential Application 快速生物传感器制造方法研究进展:以电势应用为重点

IF 4.3 3区工程技术

BioChip Journal Pub Date : 2023-11-28 DOI: 10.1007/s13206-023-00127-x

Yejin Yoon, Yein Kwon, Hanbin Park, Siyun Lee, Chulhwan Park, Taek Lee

{"title":"Recent Progress in Rapid Biosensor Fabrication Methods: Focus on Electrical Potential Application","authors":"Yejin Yoon, Yein Kwon, Hanbin Park, Siyun Lee, Chulhwan Park, Taek Lee","doi":"10.1007/s13206-023-00127-x","DOIUrl":"https://doi.org/10.1007/s13206-023-00127-x","url":null,"abstract":"The coronavirus disease pandemic has led to an urgent need for rapid and accurate viral diagnosis. Therefore, rapid biosensors, not only for viruses but also for the detection of bacteria, disease diagnosis, and environmental monitoring, have been actively researched. Biosensors analyze the binding of biomolecules and target substances mainly based on electrochemical, electrical, or optical methods. To achieve precise and rapid diagnosis, it is crucial to reduce the time required for biomolecule–target substance binding. Typically, biomolecules reach the target substances through random diffusion, and to overcome the limitations associated herewith, biosensors have been integrated with alternating current (AC) electrokinetics (ACEK) technology. ACEK, through the application of alternating voltages, converts electrical energy into fluid motion, inducing pumping, mixing, concentration, and separation of the fluid. Its low power consumption makes it highly promising as a point-of-care diagnostic device. In this paper, we review the advancements in three ACEK technologies: AC electrothermal flow, AC electro-osmosis, and AC di-electrophoresis, to discuss the development of rapid biosensor fabrication methods based on electrical potential applications.","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"19 7-8","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138514063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid and Ultrasensitive Detection of Staphylococcus aureus Using a Gold-Interdigitated Single-Wave-Shaped Electrode (Au-ISWE) Electrochemical Biosensor 金-互指单波电极(Au-ISWE)电化学生物传感器快速超灵敏检测金黄色葡萄球菌

3区工程技术

BioChip Journal Pub Date : 2023-11-14 DOI: 10.1007/s13206-023-00126-y

My-Van Tieu, Duc Trung Pham, Hien T. Ngoc Le, Thi Xoan Hoang, Sungbo Cho

引用次数: 0

A Controlled Transcription-Driven Light-Up Aptamer Amplification for Nucleoside Triphosphate Detection 三磷酸核苷检测受控转录驱动的点亮适体扩增

3区工程技术

BioChip Journal Pub Date : 2023-10-25 DOI: 10.1007/s13206-023-00124-0

Deok-Gyu Lee, Hye-Jin Lim, Ha-Yeong Lee, Dong-Myung Kim, Kyung-Ho Lee, Ju-Young Byun, Yong-Beom Shin

引用次数: 0

Enhanced Antitumor Effect of the Combination of Bacille Calmette-Guérin and an Immune Checkpoint Inhibitor in Bladder Cancer-On-a-Chip Bacille calmette - gusamrin联合免疫检查点抑制剂对膀胱癌芯片抗肿瘤作用的增强

3区工程技术

BioChip Journal Pub Date : 2023-10-17 DOI: 10.1007/s13206-023-00125-z

Se Young Choi, Mirinae Kim, Su Jeong Kang, Young Wook Choi, Sejung Maeng, Sung-Hwan Kim, In Ho Chang

引用次数: 0

Continuous Isolation of Stem-Cell-Derived Extracellular Vesicles (SC-EVs) by Recycled Magnetic Beads in Microfluidic Channels 微流控通道中再生磁珠连续分离干细胞来源的细胞外囊泡(sc - ev

3区工程技术

BioChip Journal Pub Date : 2023-10-04 DOI: 10.1007/s13206-023-00122-2

Haeun Yu, Jaejeung Kim, Jianning Yu, Kyung-A Hyun, Jae-Yol Lim, Yeo-Jun Yoon, Sunyoung Park, Hyo-Il Jung

{"title":"Continuous Isolation of Stem-Cell-Derived Extracellular Vesicles (SC-EVs) by Recycled Magnetic Beads in Microfluidic Channels","authors":"Haeun Yu, Jaejeung Kim, Jianning Yu, Kyung-A Hyun, Jae-Yol Lim, Yeo-Jun Yoon, Sunyoung Park, Hyo-Il Jung","doi":"10.1007/s13206-023-00122-2","DOIUrl":"https://doi.org/10.1007/s13206-023-00122-2","url":null,"abstract":"Stem cells produce nanosized particles known as extracellular vesicles (SC-EVs), which therapeutically affect stem cells. EVs are more abundantly produced, exhibit better stability, and possess lower immune rejection rates than stem cells. However, the traditional methods of isolating EVs, such as ultracentrifugation, possess limitations that require a complex process and consume more time. Moreover, it is difficult to isolate specific EVs that have target surface proteins that affect regenerative effects. To address these limitations, a new dual-mode horseshoe-shaped orifice micromixer (DM-HOMM) chip that can bind antibody-conjugated micromagnetic beads and SC-EVs and sequentially elute specific SC-EVs on the beads using an eluent was developed. For effective elution from the microbead-SC-EV complex, four types of eluents were used to control pH and ionic strength between antibodies and surface proteins in EVs. In addition, we investigated the reusability of antibody-conjugated micromagnetic beads. The beads indicated identical binding efficiencies between the antibodies and specific SC-EVs for three repeated cycles using the dual-mode chip. CD63+ EVs collected by the chip exhibited higher cell viability and regeneration effects than untreated and total EVs. This SC-EVs’ isolation method possesses the potential for targeted therapeutic applications and enhanced regenerative effects.","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135592564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0