Biken journal最新文献_第3页

Susceptibility of varicella-zoster virus to thymidine analogues. 水痘-带状疱疹病毒对胸腺嘧啶类似物的敏感性。

Biken journal Pub Date : 1986-03-01

H Machida

引用次数: 0

Susceptibilities of phosphonoacetic acid and acyclovir resistant varicella-zoster virus mutants to 9-beta-arabinofuranosyladenine and 1-beta-arabinofuranosylcytosine. 磷酸乙酸和无环鸟苷耐药水痘-带状疱疹病毒突变体对9- β -阿拉伯糖醛酸和1- β -阿拉伯糖醛酸胞嘧啶的敏感性

Biken journal Pub Date : 1986-03-01

K Shiraki, T Ogino, T Yamamoto, K Yamanishi, M Takahashi

引用次数: 0

Thymidine kinase with altered substrate specificity of acyclovir resistant varicella-zoster virus. 无环鸟苷耐药水痘-带状疱疹病毒底物特异性改变的胸苷激酶

Biken journal Pub Date : 1986-03-01

K Shiraki, T Ogino, K Yamanishi, M Takahashi

引用次数: 0

Activity of recombinant human alpha interferon against influenza virus infection in mice. 重组人α干扰素抗流感病毒感染小鼠的活性研究。

Biken journal Pub Date : 1985-12-01

O Sasaki, T Karaki, J Imanishi

引用次数: 0

Comparative studies on surface antigenicity of Trypanosoma cruzi trypomastigotes from infected mouse blood and infected L-cell cultures. 克氏锥虫感染小鼠血液与感染l细胞培养的锥虫表面抗原性比较研究。

Biken journal Pub Date : 1985-12-01

H Kanbara, T Nakabayashi

{"title":"Comparative studies on surface antigenicity of Trypanosoma cruzi trypomastigotes from infected mouse blood and infected L-cell cultures.","authors":"H Kanbara, T Nakabayashi","doi":"","DOIUrl":"","url":null,"abstract":"Differences in the antigenicities of surface components of blood-form trypomastigotes and trypomastigotes derived from L-cell cultures were studied by agglutination and indirect immunofluorescent tests on living parasites using various antisera from rabbits and mice. Antisera from rabbits immunized with L-cell-derived trypomastigotes and antisera obtained from rabbits infected with L-cell-derived trypomastigotes showed similar titers in both the agglutination and immunofluorescent test. Moreover, both antisera exhibited higher titers against trypomastigotes derived from L-cell cultures than against blood-form trypomastigotes. No detectable agglutination titer against either blood-form or L-cell-derived trypomastigotes was observed with sera from (a) mice infected with blood-form trypomastigotes after previous immunization with blood-form trypomastigotes, (b) mice infected with blood-form trypomastigotes and then treated with Lampit, or (c) mice infected with slightly less virulent trypomastigotes from L-cells. However, detectable and almost equal titers were observed with sera from (a), (b) and (c) in indirect immunofluorescent tests. Mouse sera also exhibited higher titers against trypomastigotes derived from L-cells than against the blood-form type. However, mouse sera showed more pronounced differences than rabbit sera. These results suggest that there may be two types of trypomastigotes in infected animals and that the surface components of blood-form trypomastigotes have lower antigenicity.","PeriodicalId":8767,"journal":{"name":"Biken journal","volume":"28 3-4","pages":"71-7"},"PeriodicalIF":0.0,"publicationDate":"1985-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15030822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Somatic mutations in the variable region of the lambda 2 chain of M315 suppression of antibodies to the idiotype of M315. M315可变区lambda 2链的体细胞突变抑制了M315独特型抗体的产生。

Biken journal Pub Date : 1985-12-01

N Sakato, T Azuma, H Fujio

引用次数: 0

Cell fusion between L-forms and protoplasts of Staphylococcus aureus. 金黄色葡萄球菌l型与原生质体的细胞融合。

Biken journal Pub Date : 1985-12-01

Y Hirachi, Y Kato, Y Toda, N Takemasa, S Kotani

{"title":"Cell fusion between L-forms and protoplasts of Staphylococcus aureus.","authors":"Y Hirachi, Y Kato, Y Toda, N Takemasa, S Kotani","doi":"","DOIUrl":"","url":null,"abstract":"Mixtures of various combinations of Lysostaphin protoplasts and stable L-forms of Staphylococcus aureus, which have different markers for drug resistance, were treated with polyethylene glycol (PEG) to examine the development of doubly resistant fusion products (fusants). To recover doubly resistant colonies as L-forms, they were incubated in 4.5% NaCl-brain heart infusion (BHI) broth containing penicillin G (PCG) for enrichment culture and cultured in PCG-4.5% NaCl-BHI agar medium (method 1), while to recover doubly resistant fusants as L-forms and coccal forms, they were grown on reversion medium (R medium) which causes reversion of protoplasts or fusants to parent type cells, and then cultured on assay media, i.e., R medium, BHI agar medium or PCG-4.5% NaCl-BHI agar medium (method 2). Under both experimental conditions, doubly resistant fusants developed as L-form cells by PEG treatment of pairs of protoplasts carrying the chloramphenicol (CP)-resistance plasmid and L-forms having chromosomal resistance to streptomycin (SM). In the reverse combinations, i.e., protoplasts showing chromosomal SM-resistance and L-form cells carrying the CP-resistance plasmid, the first method gave no doubly resistant colonies. By the second method, without enrichment culture on R medium, the latter combination gave doubly resistant fusants as L-form, coccal-type and mixed-type colonial forms, while when the PEG-treated mixture was enriched on R medium, fusants were obtained exclusively as the coccal type on either R medium or BHI agar assay medium. Neither of the methods yielded colonies of doubly resistant fusants on PEG-treatment of pairs of protoplasts and L-forms both of which were chromosomal, but with different drug resistances. These results show that PEG-induced cell fusion between protoplasts and L-forms of S. aureus, unlike the fusion between protoplasts or between L-forms, resulted in transfer of the drug resistance controlled by the plasmid to the fusion products. The fusants obtained were L-forms in method 1, and coccal type in the method 2.","PeriodicalId":8767,"journal":{"name":"Biken journal","volume":"28 3-4","pages":"59-70"},"PeriodicalIF":0.0,"publicationDate":"1985-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14950506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell fusion and cell engineering. 细胞融合和细胞工程。

Biken journal Pub Date : 1985-06-01

Y Okada

引用次数: 0

Three-year survey of the epidemiology of rotavirus, enteric adenovirus, and some small spherical viruses including "Osaka-agent" associated with infantile diarrhea. 与婴儿腹泻有关的轮状病毒、肠腺病毒和包括“大阪因子”在内的一些小球形病毒的三年流行病学调查。

Biken journal Pub Date : 1985-06-01

I Oishi, K Yamazaki, Y Minekawa, H Nishimura, T Kitaura

{"title":"Three-year survey of the epidemiology of rotavirus, enteric adenovirus, and some small spherical viruses including \"Osaka-agent\" associated with infantile diarrhea.","authors":"I Oishi, K Yamazaki, Y Minekawa, H Nishimura, T Kitaura","doi":"","DOIUrl":"","url":null,"abstract":"Studies were made by electron microscopy (EM) on the viruses associated with diarrhea of outpatients at a pediatric clinic in Osaka Prefecture during the three year period from 1980 through 1982. The viruses detected by EM by negative staining with phosphotungstic acid (PTA) were classified morphologically into 6 groups: rotavirus, adenovirus and four kinds of small spherical viruses, calicivirus, astrovirus, picornavirus/parvovirus (P/P)-like agent and Osaka-agent. Osaka-agent seems to be a newly identified small virus. It is 35-40 nm in diameter with a fringe of spike-like structures on its surface. Viruses were detected in 181 of the 395 cases of diarrhea (45.8%). Rotavirus was detected in 122 (30.9%) of the total cases and in 67.4% of the virus-positive cases, while other viruses were detected in 15% of the total cases; adenovirus in 23 (6%) and small agents in 36 (9%). Rotavirus infection showed a distinctive seasonal variation, being mainly restricted to cooler months, but infections with other viruses did not show any seasonal variation. The age distribution of patients suggested that infants of 0 to 2 years old are very susceptible to all viruses. Attempts to cultivate these viruses in vitro were successful with only two isolates of adenovirus type 5.","PeriodicalId":8767,"journal":{"name":"Biken journal","volume":"28 1-2","pages":"9-19"},"PeriodicalIF":0.0,"publicationDate":"1985-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14134227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Limited in vivo susceptibility of the South American monkey -Cebus apella- to type 1 poliovirus: physical and histopathological observations after intraspinal inoculation. 南美猴对1型脊髓灰质炎病毒有限的体内易感性:脊髓内接种后的物理和组织病理学观察。

Biken journal Pub Date : 1985-06-01

S Abe, Y Doi, R S Marchevsky, M D Leal, A Homma

引用次数: 0