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Copy number of tandem direct repeats within the inverted repeats of Marek's disease virus DNA. 马立克氏病病毒DNA反向重复序列中串联直接重复序列的拷贝数。
Biken journal Pub Date : 1986-12-01
A Kanamori, K Nakajima, K Ikuta, S Ueda, S Kato, K Hirai
{"title":"Copy number of tandem direct repeats within the inverted repeats of Marek's disease virus DNA.","authors":"A Kanamori,&nbsp;K Nakajima,&nbsp;K Ikuta,&nbsp;S Ueda,&nbsp;S Kato,&nbsp;K Hirai","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We previously reported that DNA of the oncogenic strain BC-1 of Marek's disease virus serotype 1 (MDV1) contains three units of tandem direct repeats with 132 base pair (bp) repeats within the inverted repeats of the long regions of the MDV1 genome, whereas the attenuated, nononcogenic viral DNA contains multiple units of tandem direct repeats (Maotani et al., 1986). In the present study, the difference in the copy numbers of 132 bp repeats of oncogenic and nononcogenic MDV1 DNAs in other strains of MDV1 was investigated by Southern blot hybridization. The main copy numbers in different oncogenic MDV1 strains differed: those of BC-1, JM and highly oncogenic Md5 were 3, 5 to 12 and 2, respectively. The viral DNA population with two units of repeats was small, but detectable, in cells infected with either the oncogenic BC-1 or JM strain. The MDV1 DNA in various MD cell lines contained either two units or both two and three units of repeats. The significance of the copy number of repeats in oncogenicity of MDV1 is discussed.</p>","PeriodicalId":8767,"journal":{"name":"Biken journal","volume":"29 3-4","pages":"83-9"},"PeriodicalIF":0.0,"publicationDate":"1986-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13587921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antibody responses to early antigens of varicella-zoster virus (VZV) during varicella and zoster. 水痘和带状疱疹期间对水痘-带状疱疹病毒(VZV)早期抗原的抗体反应。
Biken journal Pub Date : 1986-12-01
J Namazue, T Kato, K Yamanishi, M Takahashi, Y Asano, R Muraki
{"title":"Antibody responses to early antigens of varicella-zoster virus (VZV) during varicella and zoster.","authors":"J Namazue,&nbsp;T Kato,&nbsp;K Yamanishi,&nbsp;M Takahashi,&nbsp;Y Asano,&nbsp;R Muraki","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The antibody responses to membrane and early antigens and thymidine kinase of varicella-zoster virus (VZV) were studied in sera during both varicella and zoster by a test with fluorescent antibody to membrane antigen (FAMA), staining the biochemically transformed cells by the immunofluorescent technique and neutralization of virus-specific thymidine kinase activity, respectively. Similar increases in FAMA antibody titers were demonstrated in sera from patients with varicella and zoster. IgM was detected in both groups, but appeared earlier during varicella than during zoster. Furthermore, the antibody titers to early antigens and virus-specific thymidine kinase were higher in patients with zoster than in those with varicella. These data suggest that different types of antibody responses occur during varicella and zoster.</p>","PeriodicalId":8767,"journal":{"name":"Biken journal","volume":"29 3-4","pages":"91-7"},"PeriodicalIF":0.0,"publicationDate":"1986-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14172519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enzyme-linked immunosorbent assay for detection of serum antibody in children vaccinated with Japanese encephalitis vaccine. 酶联免疫吸附法检测乙型脑炎疫苗接种儿童血清抗体。
Biken journal Pub Date : 1986-12-01
Y Okamoto, Y Okuno, A Yamada, K Baba, H Yabuuchi
{"title":"Enzyme-linked immunosorbent assay for detection of serum antibody in children vaccinated with Japanese encephalitis vaccine.","authors":"Y Okamoto,&nbsp;Y Okuno,&nbsp;A Yamada,&nbsp;K Baba,&nbsp;H Yabuuchi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Serum antibodies in children who had been vaccinated with Japanese encephalitis (JE) vaccine were measured by enzyme-linked immunosorbent assay (ELISA) and neutralization (N) and hemagglutination-inhibition (HI) tests. Of 20 serum samples obtained after two shots of JE vaccine in the first year, all but one showed positive titers in the ELISA and N test, but five showed negative titers in the HI test. All 12 serum samples obtained after booster immunization with JE vaccine in the second year showed positive and considerably higher titers in all three tests. Moreover, a high correlation was found between the ELISA, N and HI titers. These results indicate that the ELISA is useful for detecting antibodies in subjects immunized with JE vaccine.</p>","PeriodicalId":8767,"journal":{"name":"Biken journal","volume":"29 3-4","pages":"57-62"},"PeriodicalIF":0.0,"publicationDate":"1986-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14740313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Application of Biken test (modified Elek test) for sampling of heat-stable enterotoxin of Escherichia coli isolated in Bangladesh. Biken试验(改良Elek试验)在孟加拉分离大肠杆菌热稳定性肠毒素取样中的应用。
Biken journal Pub Date : 1986-12-01
S Q Akhtar
{"title":"Application of Biken test (modified Elek test) for sampling of heat-stable enterotoxin of Escherichia coli isolated in Bangladesh.","authors":"S Q Akhtar","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The usefulness of Biken Agar 2 as a source of heat-stable toxin for the suckling mouse assay was examined. Escherichia coli (E. coli) strains isolated in Bangladesh from patients with gastroenteritis found to produce heat-stable toxin (n = 152), both heat-stable and heat-labile toxin (n = 60) and not to produce heat-stable or heat-labile toxin (n = 25) by standard suckling mice assay using broth culture were tested. Sampling from Biken Agar 2 gave comparable results to those obtained using standard broth cultures. This is the first field survey of evaluation of the Biken test for sampling heat-stable toxin of E. coli. The result further clarifies the applicability of the Biken test for sampling heat-stable toxin, and the usefulness of the Biken test for detections of heat-labile and heat-stable toxin produced by E. coli.</p>","PeriodicalId":8767,"journal":{"name":"Biken journal","volume":"29 3-4","pages":"73-5"},"PeriodicalIF":0.0,"publicationDate":"1986-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14433060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Attenuation of the virulent RH strain of Toxoplasma gondii by passages in mice immunized with Toxoplasma lysate antigens. 弓形虫裂解物抗原免疫小鼠后对弓形虫RH毒株的抑制作用。
Biken journal Pub Date : 1986-06-01
K Yano, T Nakabayashi
{"title":"Attenuation of the virulent RH strain of Toxoplasma gondii by passages in mice immunized with Toxoplasma lysate antigens.","authors":"K Yano,&nbsp;T Nakabayashi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The virulent RH strain of Toxoplasma gondii was attenuated after a few passages or just one long passage in mice immunized twice with a four-week interval between immunizations with an emulsion of Toxoplasma lysate antigens and complete Freund's adjuvant. Three avirulent strains, RH-cyst III, IV and VIII were established from the RH strain. The RH-cyst III strain was effective for vaccination against challenge with the original, virulent RH strain. The attenuation of T. gondii is an expression of the innate attributes of this parasite necessary to maintain its parasitic life cycle in nature.</p>","PeriodicalId":8767,"journal":{"name":"Biken journal","volume":"29 2","pages":"31-7"},"PeriodicalIF":0.0,"publicationDate":"1986-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14930256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Prophage type of recombinants produced by cell fusion between various combinations of lysogenic or non-lysogenic substrains from two Staphylococcus aureus L-forms. 两种金黄色葡萄球菌l型溶原性或非溶原性亚株的不同组合通过细胞融合产生的噬菌体型重组体。
Biken journal Pub Date : 1986-06-01
Y Hirachi, N Takemasa, Y Kato, Y Toda, S Kotani
{"title":"Prophage type of recombinants produced by cell fusion between various combinations of lysogenic or non-lysogenic substrains from two Staphylococcus aureus L-forms.","authors":"Y Hirachi,&nbsp;N Takemasa,&nbsp;Y Kato,&nbsp;Y Toda,&nbsp;S Kotani","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Streptomycin (SM)- or erythromycin (EM)-resistant lysogenic and non-lysogenic substrains were produced from two Staphylococcus aureus L-form strains lysogenic for different prophages, namely, EMT-L (prophage alpha) and 209P (prophage beta). Cells of these L-form substrains were fused in various combinations using polyethylene glycol (PEG), and the frequency of recombinants selected as double resistance to both SM and EM and the prophage types of these recombinants were examined. In all the combinations, the frequency of recombinants was greater when the cells were treated with PEG than when they were not, and the difference was statistically significant (p less than 0.01) in 13 combinations. Combination between the lysogenic SM-resistant EMT-L substrain [EMT(Smr-alpha)] and lysogenic EM-resistant 209P-L substrain [209P(Emr-beta)] and the reverse combination, between 209P(Smr-beta) and EMT(Emr-alpha), resulted in a majority of recombinants harboring prophage beta. The former combination yielded recombinants that all held both prophage alpha and beta.</p>","PeriodicalId":8767,"journal":{"name":"Biken journal","volume":"29 2","pages":"45-9"},"PeriodicalIF":0.0,"publicationDate":"1986-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14085852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of the composition of reversion medium on change of Staphylococcus aureus lysostaphin protoplasts to coccal forms and L-forms. 还原培养基组成对金黄色葡萄球菌溶葡萄蛋白原生质体向球菌型和l型转变的影响。
Biken journal Pub Date : 1986-06-01
Y Kato, Y Hirachi, Y Toda, N Takemasa, S Kotani
{"title":"Effect of the composition of reversion medium on change of Staphylococcus aureus lysostaphin protoplasts to coccal forms and L-forms.","authors":"Y Kato,&nbsp;Y Hirachi,&nbsp;Y Toda,&nbsp;N Takemasa,&nbsp;S Kotani","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The experimental conditions under which protoplasts of Staphylococcus aureus strain MS353 (pCp) are converted to the coccal or L-form were investigated. Protoplasts prepared by treating coccal MS353 (pCp) strain with Lysostaphin formed various types of colonies (coccal form, L-form and mixed types) in about 50% yield when they were plated on reversion (R) medium consisting of 2% brain heart infusion, 0.5M sodium succinate, 0.01% bovine serum albumin, 20 mM MgCl2 and 0.6% agar. The L-form type colonies with a typical fried-egg appearance that developed on the R medium at an early stage gradually reverted to the coccal form through a mixed type stage in which a high density area first appeared in the periphery of the colony and then spread throughout the colony. The use of modified R medium without MgCl2 or R medium in which 0.5M sodium succinate as an osmotic stabilizer was replaced by 7.5% NaCl resulted in marked delay in the appearance of reverted cells. R medium without bovine serum albumin yielded atypical L-form type colonies, which contained masses of coccal cells with very irregular margins. On the other hand, R medium without MgCl2 but with penicillin G supported development of L-form type colonies at high rate (13-15%) from the inoculated protoplasts.</p>","PeriodicalId":8767,"journal":{"name":"Biken journal","volume":"29 2","pages":"39-44"},"PeriodicalIF":0.0,"publicationDate":"1986-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14930257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antibody-mediated enhancement of infection by dengue virus of the P815 murine mastocytoma cell line. 抗体介导的登革热病毒对P815小鼠肥大细胞瘤细胞系感染的增强作用。
Biken journal Pub Date : 1986-06-01
L F Legrand, H Hotta, S Hotta, M Homma
{"title":"Antibody-mediated enhancement of infection by dengue virus of the P815 murine mastocytoma cell line.","authors":"L F Legrand,&nbsp;H Hotta,&nbsp;S Hotta,&nbsp;M Homma","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Dengue type 2 virus (DEN 2) could replicate only to a limited extent in a murine mastocytoma cell line, P815. The viral multiplication was enhanced 10- to 100-fold by mouse anti-DEN 2 antiserum or anti-DEN 2 type-specific monoclonal antibody diluted beyond their neutralizing titers. Cells incubated with virus-antibody mixtures changed morphologically, developing a mature mast cell-like appearance, 4-5 days after infection. The indirect fluorescent antibody technique showed that the enhancement of infection was caused by an increase in the number of DEN 2-infected cells. This is the first report that cells of mast cell lineage support dengue virus multiplication, and that virus production is enhanced in the presence of anti-dengue antibodies.</p>","PeriodicalId":8767,"journal":{"name":"Biken journal","volume":"29 2","pages":"51-5"},"PeriodicalIF":0.0,"publicationDate":"1986-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14233243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of a micro-neutralization test for chikungunya virus. 基孔肯雅病毒微中和试验的研制。
Biken journal Pub Date : 1986-03-01
E Raharjo, M Tadano, Y Okamoto, Y Okuno
{"title":"Development of a micro-neutralization test for chikungunya virus.","authors":"E Raharjo,&nbsp;M Tadano,&nbsp;Y Okamoto,&nbsp;Y Okuno","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A rapid, micro-scale focus reduction neutralization test for chikungunya virus was developed. In the test, cell monolayers are prepared in a 96-well tissue culture plate and the PAP (peroxidase-antiperoxidase) staining technique is used for detection of foci of chikungunya virus infected cells. This test is suitable for rapid diagnosis and epidemiological studies of the virus.</p>","PeriodicalId":8767,"journal":{"name":"Biken journal","volume":"29 1","pages":"27-30"},"PeriodicalIF":0.0,"publicationDate":"1986-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14155484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A long-term follow-up study on the efficacy of further attenuated live measles vaccine, Biken CAM vaccine. 进一步减毒麻疹活疫苗Biken CAM疫苗疗效的长期随访研究
Biken journal Pub Date : 1986-03-01
S Isomura, T Morishima, K Nishikawa, N Hanada, M Rahman, M Terashima, S Kido, S Ueda, M Takahashi
{"title":"A long-term follow-up study on the efficacy of further attenuated live measles vaccine, Biken CAM vaccine.","authors":"S Isomura,&nbsp;T Morishima,&nbsp;K Nishikawa,&nbsp;N Hanada,&nbsp;M Rahman,&nbsp;M Terashima,&nbsp;S Kido,&nbsp;S Ueda,&nbsp;M Takahashi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Antibody persistence was measured in 39 children in an open community 12-13 years after immunization against measles with further attenuated live vaccine, Biken CAM. Serum samples of the children taken every two or three years after vaccination had higher, lower, or the same HI antibody titers as those in samples taken 6 weeks after vaccination. These differences reflected a decrease in the titer in some children and subclinical natural reinfection in others. However, all the children still retained detectable antibody in 12 or 13 years after vaccination, indicating long-term persistence of immunity after immunization with Biken CAM vaccine. For evaluation of the protective efficacy of the vaccine, matched controls were studied during the same period. Serological examination revealed that 97.5% of the controls were infected with measles and contracted the disease. In contrast, none of the vaccinees developed clinical infection after close contact with measles patients.</p>","PeriodicalId":8767,"journal":{"name":"Biken journal","volume":"29 1","pages":"19-26"},"PeriodicalIF":0.0,"publicationDate":"1986-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14896455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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