Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology最新文献

{"title":"[Cultivation and purification of high proliferative potential endothelial progenitor cells from murine bone marrow].","authors":"Wen Biao Zhu,&nbsp;Hai Lin Zhu,&nbsp;Jing Jing Wu,&nbsp;Bao He Wang,&nbsp;Qi Ru Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The present study was designed to culture and purify high proliferative potential endothelial progenitor cells (HPP-EPCs) from murine bone marrow and their progeny cells using a culture system which has been established by us recently, in order for more intensive researches on these cells. Fresh murine bone marrow cells were preplated to allow the preferential attachment of non-EPCs to tissue culture plates and then unattached cells were repreplated in the culture system containing the bone marrow endothelial cell line-conditioned medium (BMEC-CM). The colonies containing about 2 x 10(4) cells were collected respectively and single colony-derived cells were cultured for their expansion in the above-mentioned culture system. These single colonies were able to yield 8 x 10(6) progeny cells. The endothelial-lineage characteristics of expanded cells were confirmed by immunofluorescent staining for CD31 and vWF, FITC-UEA-1 binding and Dil-Ac-LDL uptake. These data suggest that murine bone marrow HPP-EPCs can proliferate exuberantly so that their progeny cells can be obtained with high yield by using the single colony-derived cell culture in the presence of BMEC-CM.</p>","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"42 3-4","pages":"237-43"},"PeriodicalIF":0.0,"publicationDate":"2009-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28355864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"C/EBPbeta sustains undifferentiated state of murine embryonic stem cells as a mediator of LIF.","authors":"Li Sun,&nbsp;Hai Zhen Wang,&nbsp;Ying Zhang,&nbsp;Ding Gan Liu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>C/EBPbeta (also called NF-IL6) is a multifunctional transcription factor, a major function of which is the enhancement of cellular differentiation. Leukemia inhibitory factor (LIF) is a cytokine playing divergent roles in different cell types: induces differentiation in preadipocytes and, however, inhibits differentiation in pluripotent murine embryonic stem (mES) cells. However, roles of C/EBPbeta in mES cells are obscure. Here, we show for the first time that C/EBPbeta in the presence of LIF plays a role of sustaining undifferentiated state in this cell line, i.e. C/EBPbeta is a target of regulation of LIF, as described as follows. The expression of endogenous C/EBPbeta proteins in mES cells is positively correlated with the LIF added into medium. Even the exogenous C/EBPbeta proteins and their truncated form, LIP, artificially overexpressed in undifferentiated mES cells, do not enhance but inhibit ES cells' differentiation in the presence of LIF, and the long isoforms of C/EBPbeta proteins strongly enhance cell propagation. This enhancement lasts for a certain short time even after LIF removal. In the absence of LIF, C/EBPbeta and LIP induce expression of differentiation-related genes and promote mES cells' differentiation, as anticipated. With LIF, the expression levels of some differentiation-related genes regulated by C/EBPbeta and LIP were significantly lower than that without LIF. Therefore, in pluripotent mES cells C/EBPbeta is regulated by LIF and sustains undifferentiated state of those cells as a mediator of LIF.</p>","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"42 2","pages":"127-36"},"PeriodicalIF":0.0,"publicationDate":"2009-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28327009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A simplified RNA extraction method to study signal transduction in tobacco pollen tube growth.","authors":"Hua Hong Fang, Zhao Wu Ma, Fen Li, Guang Hui Yu","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"42 2","pages":"173-8"},"PeriodicalIF":0.0,"publicationDate":"2009-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28327014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Cytological and SSR analysis of irradiation-induced progenies of Trititrigia substitution line SN0095].","authors":"Yin Guang Bao,&nbsp;Xing Feng Li,&nbsp;Hao Zong,&nbsp;Chun Hua Zhao,&nbsp;Fa Cui,&nbsp;Yu Hai Wang,&nbsp;Hong Gang Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Pollens of Trititrigia substitution line SN0095 were radiated with 60Co-gamma ray and cytological characters of the progenies M1 and M2 were studied. The results showed that variations with different frequencies and chromosome numbers (2n=41, 2n=40 and 2n=39) were observed in both M1 and M2. Abnormal phenomena, such as univalents, multivalents, chromosome fragments, laggard chromosomes, chromosome bridges and micronucleus, appeared in high frequencies during the meiosis. It suggested that irradiation could promote changes of chromosome number and configuration efficiently, and could lead to rearrangements or translocations between chromosomes. The M2 progenies were examined using BARC159(240), a Thinopyrum intermedium-specific SSR marker of SN0095. Most M2 plants carried the locus BARC159(240), which indicated that the specific segment involving the locus BARC159(240) of Thinopyrum intermedium existed in these plants. However, a band of common wheat YN15 disappeared in a few plants although the locus BARC159(240) existed, which implied that chromosome rearrangements may have occurred in these plants.</p>","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"42 2","pages":"89-94"},"PeriodicalIF":0.0,"publicationDate":"2009-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28251071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Modality and structure of Japanese eel (Anguilla japonica) during spermiogenesis].","authors":"Long Zhen Zhang,&nbsp;Ping Zhuang,&nbsp;Zhen Guo Qiao,&nbsp;Jian Yi Liu,&nbsp;Xiao Rong Huang,&nbsp;Tao Zhang,&nbsp;Guang Peng Feng,&nbsp;Feng Zhao","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The special process and special structure which bring organelle during the spermiogenesis of Japanese eel, Anguilla japonica were observed by scanning electron microscope and transmission electron microscope. The process which spermatoblast became sperm including four special stages, the early stage, the middle stage, the later stage and the spermic stage, then came into being a normal mature sperm. In the early stage, cell nucleus became long form gradually by the oval form. In one side of the cell nucleus, there was a big and special globoid structure dyeing lower, account for 1/3 of cell nucleus cubage. It contains a little of deep dyeing grain form and the lines form material, the outside is wrapped by plasmalemma separated with the cell nucleus, the outside of that structure and cell nucleus still lay a plasmalemma. The spermiogenesis of early stage did not form independent centriolar complex and mitochondria. In the middle stage, the cell nucleus presented a long form with the globoid structure on the top of the nucleus, and the down side had no globoid structure where the flagellum primordium appears. The globoid structure changed with the spermiogenesis. The inner part of the globoid differentiated a centriolar complex and mitochondria step by step. The lysosomes distributed in the medium segment of the cell nucleus obviously. In the late stage, the cell nucleus was similar with the shape of eyebrow or crescent. The centriolar complex released from the globoid structure, then became an independent structure. There were mitochondria which had not become the independent structure still in the globoid structure. Under the karyon, there was flagellum primordium where sent a rather long flagellum. The flagellum formed a typical \"9+0\" microtubular structure at that time. The spermatozoa in this phase has movable ability. In the spermic stage, the cell nucleus was round in shape. The centriolar complex was inside implantation fossa. Mitochondria were under karyon. And under the mitochondria was the central space of the sleeve. The flagellum formed a typical \"9+2\" microtubular structure at that time. The spermatozoa of Japanese eel, A. japonica became complete mature spermatozoa must pass through four phases for abnormality.</p>","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"42 2","pages":"156-64"},"PeriodicalIF":0.0,"publicationDate":"2009-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28327012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The formation and symplastic transport function of ectodesmata-like in garlic during the parenchyma cells declining].","authors":"Yu Dong,&nbsp;Na Liu,&nbsp;Zhong He Wang,&nbsp;Dong Mei Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Using fluorescence and electron microscopy, the ultrastructure and symplastic transport function of Ectodesmata-like (ED-like) were studied in the declining parenchyma cells of garlic during germination period. The results showed that plasmodesmata (PD) were gradually stretched and broke into ED-like. The diameter of PD and the diameter of appressed endoplasmic reticulum (AER) in PD underwent a series of changes. F-actin and myosin might take part in the regulation of the symplastic transport of the ED-like.</p>","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"42 2","pages":"165-72"},"PeriodicalIF":0.0,"publicationDate":"2009-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28327013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Analysis of the level and pattern of genomic DNA methylation in different ploidy watermelons by MSAP (Citrullus lanatus)].","authors":"Chun Guo Wang,&nbsp;Yu Gu,&nbsp;Cheng Bin Chen,&nbsp;Ding Liang Jiao,&nbsp;Zhen Yi Xue,&nbsp;Wen Qin Song","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>DNA methylation is one of the major epigenetic modifications. It is very important to the regulation of gene expression. In present study, an autoploidy series (2x, 3x and 4x) in watermelon (Citrullus lanatus) was constructed and MSAP (Methylation-Sensitive Amplification Polymorphism) analysis was conducted to elucidate the level and pattern of DNA methylation at CCGG sites in different ploidy watermelons. Totally, 1883 genetic loci were produced by 23 pairs of selective primers, of which 647, 655 and 581 sites were detected in diploid, autotriploid and autotetraploid, respectively. The methylation sites were 181, 150 and 159, and the corresponding total methylation ratios were 28.0%, 22.9% and 27.4% in 2x, 3x and 4x, respectively, of which the fully methylation sites were 121, 80 and 82, and the corresponding fully methylation ratios were 18.7%, 12.2% and 14.1%. Further analysis of the pattern of DNA methylation suggested that compared 4x with 2x, about half of detected sites (54.4%) shown changes of DNA methylation patterns. Similarly, compared 4x with 3x, 45.4% sites also shown changes of DNA methylation patterns. Moreover, the trend of DNA methylation adjustment mainly involved increase of DNA methylation levels in 4x. However, compared 3x with 2x or 4x, although the changes of DNA methylation pattern also widely occurred, which involved 41.6% (compared 3x with 2x) and 45.4% (compared 3x with 4x) sites, respectively, the trend of DNA methylation adjustment mainly involved decrease of DNA methylation levels in 3x. All these results indicated that DNA methylation events were widely existed in different ploidy watermelons. However, not only based on the total DNA methylation ratio or fully DNA methylation ratio, the results both implied that the DNA methylation levels were not closely associated with the autopolyploidy level in watermelon. Autotriploid watermelon shows obvious low level of DNA methylation. Analysis of DNA methylation patterns also suggested that the adjustment of DNA methylation patterns in autotriploid mainly involved demethylation events, implying the unusual characteristic of DNA methylation status in 3x watermelon. The present results are valuable to further explore the nature of triploid vigor and autopolyploidizaion in watermelon from the view of epigenetics.</p>","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"42 2","pages":"118-26"},"PeriodicalIF":0.0,"publicationDate":"2009-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28251075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The myocardial protective effects of puerarin on STZ-induced diabetic rats.","authors":"Zhen Yu Pan,&nbsp;Zhao Sheng Bao,&nbsp;Zhong Min Wu,&nbsp;Xu Ming Wang,&nbsp;Jing Zhang Zheng,&nbsp;Yue Liang Shen,&nbsp;Xiao Ming Zhang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>To investigate the myocardial protective effects of puerarin on streptozotocin (STZ)-induced diabetic rats and the possible mechanism were involved. 45 Sprague-Dawley male rats were randomly divided into 3 groups as diabetic group (intraperitoneally injected STZ 65 mg/kg), puerarin treatment group (intraperitoneally injected STZ 65 mg/kg, and intraperitoneally injected puerarin 100 mg/kg/day for 4 weeks), and control group (intraperitoneally injected saline 6 ml/kg). Four weeks after the model induction, the myocardial changes were observed by H-E stain and Transmission electron microscopy, the alteration of thrombospondin-1 (TSP-1) protein and mRNA expression in the myocardium were also assessed by immunohistochemistry and real-time PCR. The heart function of three groups' rats was tested by Langendorff isolated in vivo heart perfusion. The differences in the data of weight and blood sugar of diabetic between puerarin treatment and normal groups were significant after 4 weeks (P<0.01). Our results demonstrated that diabetic myocardial ultrastructural changes included myofibrillar disarrangements and mitochondria disruption. These damages were significantly less severe in the puerarin treatment group compared with the diabetic group. A significant decrease of TSP-1 expression was observed in the puerarin treated rats' myocardium compared to the diabetic rats (P<0.01). Left ventricular systolic end pressure (LVSEP) and left ventricular developed pressure (LVDP) of puerarin treatment group were also significantly increased compared to diabetic group (P<0.01). Altogether puerarin could improve the left ventricular function of diabetic rats and showed protective effects of myocardium by decreasing the TSP-1 expression in myocardium of diabetic rats.</p>","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"42 2","pages":"137-44"},"PeriodicalIF":0.0,"publicationDate":"2009-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28327010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gene expression profiling of mice liver tissue after intragastric administration of Chinese nutgall extract.","authors":"Feng Mei Han,&nbsp;Xiao Ming Zhang,&nbsp;Qi Song Xia,&nbsp;Jun Jun Wang,&nbsp;Yong Chen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Kunming mice (male, weight 20 +/- 2g) were daily intragastric administration of Chinese nutgall extract (0.2 mL/10 g body weight, equal to 8 g Chinese nutgall material/1 kg body weight) for 30 days. Liver tissue mRNA were extracted from normal control group and Chinese nutgall treated group respectively, then were reversely transcribed to cDNA with dUTP labeled by different fluorescence (Cy3, Cy5) as hybridization probes. Both of the cDNA probes were mixed equally in 20 microL of hybridization solution and hybridized with mice complete genome oligonucleotide microarray. The fluorescent signals were acquired by laser scanner and analyzed by GenePix Pro 4.0 software. The biological function analysis and the pathway analysis of differentially expressed genes were performed according to Gene Ontology database. As a result, there were 461 genes differentially expressed in Chinese nutgall treated group, in which 373 genes were function-known and the others were function-unknown. Among the 461 genes, 267 genes were up-regulated and the others were down-regulated. The differentially expressed genes were involved in metabolism, DNA binding and transcription, protein synthesis and modification, cell cytoskeleton and cell adhesion, cell cycle and differentiation, ion channels and transporters, signal transduction, immune response and apoptosis of liver cell. The presented work might be quite important for understanding the pathogenic mechanisms of liver injury induced by Chinese nutgall.</p>","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"42 2","pages":"101-8"},"PeriodicalIF":0.0,"publicationDate":"2009-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28251073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[NO may function in the downstream of Ca2+ in ethylene induced stomatal closure in Vicia faba L].","authors":"Guo Hua Liu,&nbsp;Jing Liu,&nbsp;Li Xia Hou,&nbsp;Jing Tang,&nbsp;Xin Liu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Through pharmacological combined with laser scanning confocal microscope (LSCM) and spectrophotography to study the role of Ca2+ and NO in signaling during Vicia faba L. stomatal movement response to ethylene (Eth). The results showed that treatment with ethephon (0.004%, 0.04%, 0.4%) resulted in a time- and dose-dependent stomatal closure under light. NO scavenger cPTIO, nitrate reductase inhibitor NaN3, or extracellular Ca2+ chelation EGTA reduced ethylene-induced stomatal closure. Moreover, ethylene was shown to enhance nitric oxide levels and, corresponding, nitrate reductase activity. Inhibition of the nitrate reductase diminished ethylene-induced NO production in both stomatal guard cell and leaf. Finally, ethylene-induced NO levels and nitrate reductase activity decreased when Ca2+ was compromised. On the basis of biochemical and pharmacological experimental results, we can conclude that Ca2+ and NO were involved in the signal transduction pathway of ethylene induced stomatal closure. Nitrate reductase-derived NO may represents a novel downstream component of Ca2+ signaling cascade during ethylene-induced stomatal movement in Vicia faba L.</p>","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"42 2","pages":"145-55"},"PeriodicalIF":0.0,"publicationDate":"2009-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28327011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}