Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology最新文献

[Effects of (-)-epigallocatechin-3-gallate on interleukin-1beta expression in mouse wound healing]. [(-)-表没食子儿茶素-3-没食子酸酯对小鼠伤口愈合中白细胞介素-1 β表达的影响]。

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2009-06-01

Yu Jia Shen, Wei Guo Li, Hui Zhang, Bin Li, Hong Xu Yuan, Jiang Jiang Zhang

引用次数: 0

[bFGF-stimulated MEF-conditioned medium is capable of maintaining human embryonic stem cells]. [bfgf刺激的mef条件培养基能够维持人类胚胎干细胞]。

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2009-06-01

Yi Ping Zhou, Anne Rochat, Antoinette Hatzfeld, Isabelle Peiffer, Romain Barbet, Jacques Hatzfeld, Ma Lin Li

引用次数: 0

[The expression level of FtsZ protein during the dedifferentiation and redifferentiation of explants of Nicotiana tobacum]. [烟草外植体脱分化和再分化过程中 FtsZ 蛋白的表达水平]。

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2009-06-01

Qi Yi Lei, Huan Zhao, Jiang Li Zhang, Yong Hu, Xiang Lin Liu

引用次数: 0

[Oral immunization of Lugurus lugurus with the plasmid nanoemulsion of zona pellucida 3 to enhance infertility effects]. [透明带3质粒纳米乳口服免疫褐家鼠增强不育效果]。

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2009-06-01

Ai Lian Zhang, Shu Zhen Zhuang, Dao Cheng Wu, Yi Jie Li, Fu Chun Zhang

{"title":"[Oral immunization of Lugurus lugurus with the plasmid nanoemulsion of zona pellucida 3 to enhance infertility effects].","authors":"Ai Lian Zhang, Shu Zhen Zhuang, Dao Cheng Wu, Yi Jie Li, Fu Chun Zhang","doi":"","DOIUrl":"","url":null,"abstract":"Immunocontraceptive technology is beginning to attract more attention for the control of wild mouse or other pest animals. Oral vaccination of animals and man, to provide effective mucosal and/or systemic immunity, is a good delivery system, but it remains poor bioavailablity, one of the most promising strategies to improve the oral delivery relies on polymeric nanoparticles carriers to encapsulate vaccine. Nanoemulsion was used as the carriers of oral immunization of Lugurus Lugurus zona pellucida 3, the immune response and antifertility potential of this vaccine is valuated. Nanoemulsion-encapsulated plasmid pcDNA3.0-Aat-COMP-lzp3-C3d3 (pACLC DNA vaccine) was prepared and evaluated the encapsulation efficiency by anion exchange chromatography. The mice were orally delivered with nanoemulsion-encapsulated plasmid, to detect specific IgG in serum and IgA in vaginal fluid by indirect ELISA, and to perform fertility experiment, to do histological analysis of immunized mice ovary. The results showed that the specific IgG and IgA antibody is elicited, the fertility rate is deceased at a certain extent. In conclusion, it is feasible that nanoemulsion is used as the carriers of oral immunization.","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"42 3-4","pages":"186-92"},"PeriodicalIF":0.0,"publicationDate":"2009-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28355399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Clone and development of RAPD and SCAR markers linked to sex determination in the dioecious species Humulus scandens L]. [雌雄异株葎草性别决定相关RAPD和SCAR标记的克隆与发育]。

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2009-06-01

Wu Jun Gao, Fu Cong Sun, Wei Zhi Yin, Yan Ke Ji, Chuan Liang Deng, Long Dou Lu

引用次数: 0

[The expression of MKP-1 and p-ERK(1/2) in primary ovarian epithelial tumor tissues]. [MKP-1和p-ERK(1/2)在卵巢原发上皮肿瘤组织中的表达]。

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2009-06-01

Jian Wei Zhou, Ning Yue Gan, Wei Jiang Zhang

{"title":"[The expression of MKP-1 and p-ERK(1/2) in primary ovarian epithelial tumor tissues].","authors":"Jian Wei Zhou, Ning Yue Gan, Wei Jiang Zhang","doi":"","DOIUrl":"","url":null,"abstract":"To investigate the expression of mitogen activated protein kinase phosphatase-1 (MKP-1) and phosphorylation extracellular signal-regulated kinases (p-ERK(1/2)) in primary ovarian epithelial tumor tissues, and provide experiment's foundation on the new treatment in ovarian cancer. Expression of MKP-1 and p-ERK(1/2) in tissues from 64 patients with primary ovarian epithelial tumor, 35 patients with ovarian epithelial bordline tumor, 32 patients with ovarian epithelial benign tumor and 26 normal ovarian tissues was detected by immunohistochemistry. Western-blot was also used for detecting the expression of MKP-1 and p-ERK(1/2) protein in these tissues. Immunohistochemistry and Western-blot assay showed that the expression of MKP-1 was gradually decreased in normal ovarian tissues, benign tumor, bordline tumor and carcinoma respectively, and there were significant differences among them (P < 0.01). The MKP-1 expression level in the carcinoma tissues of stage III/IV patients was significantly lower than that of stage I/II patients. However, the expression of p-ERK(1/2) was gradually increased in normal ovarian tissues, benign tumor, bordline tumor and carcinoma respectively, and there were also significant differences among them (P < 0.01), the p-ERK(1/2) expression level in the carcinoma tissues of stage III/IV patients was significantly higher than that of stage I/II patients. Expression of MKP-1 and p-ERK(1/2) in same ovarian carcinoma tissues detected by immunohistochemistry and Western-blot assay showed significant negative correlation (r = -0.90, P < 0.01 and r = -0.78, P < 0.01 respectively). The expression changes of MKP-1 and ERKs may play a role in the development of ovarian carcinoma. The abnormal expression of MKP-1 and p-ERK(1/2) probably assists in promoting the development and progression of ovarian carcinoma.","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"42 3-4","pages":"224-30"},"PeriodicalIF":0.0,"publicationDate":"2009-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28355404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The expression pattern and possible function of microRNA-34a in neurons]. [microRNA-34a在神经元中的表达模式及可能的功能]。

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2009-06-01

Yan Ting Qi, Yu Zhao, Zhi Zhang, Dan Li, Ji Yi Gu, Xiao Nan Fu, Xiao Na Chen, Chong Gang Yuan, Li Hui Lai

引用次数: 0

[Effect of incubation conditions on the nacre secretion capacity of in vitro epithelium from mantle of Hyriopsis cumingii]. [培养条件对三角帆蚌(Hyriopsis cumingii)外膜珠质分泌能力的影响]。

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2009-06-01

Yong Fu Chen, Guo Ying Qian

{"title":"[Effect of incubation conditions on the nacre secretion capacity of in vitro epithelium from mantle of Hyriopsis cumingii].","authors":"Yong Fu Chen, Guo Ying Qian","doi":"","DOIUrl":"","url":null,"abstract":"The effects of different calcium ion concentration in culture medium of epithelim, varient pH, and nucleus rotundus present or absent on the calcium metabolism of epithelim from mantle of Hyriopsis cumingii work were investigated in this study. With the variance of calcium ion concentration and pH, the results obtained by using polarizing microscope showed that there was no significant difference on calcium ion containing of epithelim cells between nucleus rotundus present and absent. Meanwhile, the reproduction of epithlim cell in mantle was occurred after incubation and it was further transferred to form pearl sac. The complete pearl sac is formed when connective tissue cells around epithlim cell of pearl sac. An obvious birefringence was observed by using polarizing microscope and its strength was positively affected by the incubation duration and calcium ion concentration of culture medium. The results demonstrated that with the development of cell and tissue of mantle, the absorption of calcium carbonate from culture medium was occurred and there existed the significant correlation between surrounding calcium ion concentration and absorption or secretion of cultures. The largest quantity of calcium sphere was occurred when the calcium ion concentration was 200 ml/L, which was very significantly higher than that of others (p < 0.01). It was also found that the calcium ion absorption was affected by the culture tissue and the status of culture. Higher calcium ion requirement was in primary culture than that in subculture. The calcium ion absorption and secretion was higher in tissue gobbet than that in single cell. A high significant correlation was observed between calcium ion absorption (Y) and pH (X): Y = 52.34 - 5601.23 X (r = 0.9661, p < 0.05). The calcium ion concentration 200mg/L and pH 7.2-7.5 are suggested for incubation conditions of epithelim primary culture in mantle of Hyriopsis cumingii.","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"42 3-4","pages":"249-55"},"PeriodicalIF":0.0,"publicationDate":"2009-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28355866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Histological and cytological study on the ontogeny of embryoid in pepper anther culture]. 辣椒花药培养胚状体发生的组织学和细胞学研究。

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2009-06-01

Ju Ping Zhang, Zhen Hui Gong, Xing Zhi Zhang, Jun E Wang

{"title":"[Histological and cytological study on the ontogeny of embryoid in pepper anther culture].","authors":"Ju Ping Zhang, Zhen Hui Gong, Xing Zhi Zhang, Jun E Wang","doi":"","DOIUrl":"","url":null,"abstract":"The characteristics of histology and cytology of embryogenesis in pepper anther culture were examined with fluorescence microscopy, scanning microscopy, and electron microscopy. Pepper was characterized by a strong asynchrony of pollen development within a single anther. With the change of culture period, the proportion of dead pollen increased drastically from 2 day after culture. Microspores that were cultured at the late-uninucleate stage followed one of two developmental pathways. In the more common route, the first sporophytic division was asymmetric and produced what appeared to be typical bicellular pollen. Embryogenic pollen was formed by repeated divisions of the vegetative nuclei. An exine with its specific pattern had already been formed, when microspores were released from tetrads. During subsequent pollen development, microspores increased in size and continued to strengthen the exine. After 24 h in culture, the microspores had increased in size. Thereafter, embryogenesis was indicated in some microspores by two different morphological changes. One featured an expansion in volume of the cell cluster around the germination aperture, the other showed cell cluster volume expansion over the entire microspore surface. Morphogenesis of microspore-derived embryos has been analyzed, at both light and electron microscopical levels. The changes in cell organization after embryogenesis induction, and the characterization of the time sequence of a set of structural events, had been also explained. These changes mainly affected the plastids, the vacuolar compartment, the cell wall and the nucleus. Further differentiation processes mimicked that of the zygotic development.","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"42 3-4","pages":"200-10"},"PeriodicalIF":0.0,"publicationDate":"2009-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28355401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Identification of a novel male-specific DNA marker in loach (Misgurnus anguillicaudatus)]. [泥鳅(Misgurnus anguillicaudatus)雄性特异性DNA标记的鉴定]。

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2009-06-01

Jin Hua Zhi, Shao Le Kang, Xiao Hua Xia, Qi Yan Du, Zhong Jie Chang

{"title":"[Identification of a novel male-specific DNA marker in loach (Misgurnus anguillicaudatus)].","authors":"Jin Hua Zhi, Shao Le Kang, Xiao Hua Xia, Qi Yan Du, Zhong Jie Chang","doi":"","DOIUrl":"","url":null,"abstract":"The loach is widely distributed in China and cultivated enormously with the price increased in recent years. Its sex determination type and sex chromosome has not been recognized. In the present study, we report a novel sex-specific DNA marker in loach Misgurnus anguillicaudatus. The animals used in our experiments were collected from a wild population in wetland on the ancient Yellow river, Yanjin Country, Henan Province. 220 random primers were used for random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) with the genomic DNA from two sex individuals as template. When using the S2111 primer, a novel 1040 bp sex-specific PCR product was amplified from known male fish. This fragment of DNA was cloned and sequenced. Two primers (MAmf1-P1 and -P2) were designed from the cloned sex-specific sequence to amplify the male-specific fragment using PCR for sexing, with the house-keeping gene beta-actin as positive control. Sex-specific bands in the gel were represented in the males but none were found in the females. The PCR products in the gel were then transferred onto nylon membranes and hybridized with a DIG-labeled probe of the cloned male-specific DNA fragment. Clear hybridization signals were found only in the male loach. The same result was obtained from dot blotting hybridization. The fragment is a repetitive DNA, and presents its closely related species Paramisgurnus dabryanus but not linked to sex. The cloning of the male-specific DNA laid a good foundation for the isolation of gender-specific chromosome segments with chromosomal walking or gene library, and improved the mechanism exploration of sex chromosome evolution in fish.","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"42 3-4","pages":"231-6"},"PeriodicalIF":0.0,"publicationDate":"2009-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28355405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0