[Cytological and SSR analysis of irradiation-induced progenies of Trititrigia substitution line SN0095].

Abstract

Pollens of Trititrigia substitution line SN0095 were radiated with 60Co-gamma ray and cytological characters of the progenies M1 and M2 were studied. The results showed that variations with different frequencies and chromosome numbers (2n=41, 2n=40 and 2n=39) were observed in both M1 and M2. Abnormal phenomena, such as univalents, multivalents, chromosome fragments, laggard chromosomes, chromosome bridges and micronucleus, appeared in high frequencies during the meiosis. It suggested that irradiation could promote changes of chromosome number and configuration efficiently, and could lead to rearrangements or translocations between chromosomes. The M2 progenies were examined using BARC159(240), a Thinopyrum intermedium-specific SSR marker of SN0095. Most M2 plants carried the locus BARC159(240), which indicated that the specific segment involving the locus BARC159(240) of Thinopyrum intermedium existed in these plants. However, a band of common wheat YN15 disappeared in a few plants although the locus BARC159(240) existed, which implied that chromosome rearrangements may have occurred in these plants.