Nanotechnology and Precision Engineering最新文献_第4页

A novel microfluidic flow-cytometry for counting numbers of single-cell β-actins 一种用于计数单细胞β-肌动蛋白的新型微流控流式细胞术

Nanotechnology and Precision Engineering Pub Date : 2020-09-01 DOI: 10.1016/j.npe.2020.06.001

Lixing Liu , Beiyuan Fan , Hongyu Yang , Deyong Chen , Shuang Zhang , Junbo Wang , Jian Chen

{"title":"A novel microfluidic flow-cytometry for counting numbers of single-cell β-actins","authors":"Lixing Liu , Beiyuan Fan , Hongyu Yang , Deyong Chen , Shuang Zhang , Junbo Wang , Jian Chen","doi":"10.1016/j.npe.2020.06.001","DOIUrl":"10.1016/j.npe.2020.06.001","url":null,"abstract":"<div>As a house keeping protein with stable expressions, β-actin is used as a loading control in normalization of western blotting. However, the actual numbers of β-actins at the single-cell level remain elusive. Based on a home-developed flow cytometry, single-cell numbers of β-actin from 8 cell types (subtypes) and 2 tumour patient samples were quantified as 9.62 ± 4.29 × 105 (A549, Ncell = 14,242), 6.46 ± 3.34 × 105 (Hep G2, Ncell = 35,932), 1.58 ± 0.90 × 106 (MCF 10A, Ncell = 16,650), 1.08 ± 0.48 × 106 (HeLa, Ncell = 26,151), 7.60 ± 4.34 × 105 (PC3, Ncell = 11,922), 1.10 ± 0.72 × 106 (SACC-83, Ncell = 13,616), 8.58 ± 4.54 × 105 (CAL 27, Ncell = 7271), 9.00 ± 4.69 × 105 (CAL 27-LN2, Ncell = 6222), 8.26 ± 4.48 × 105 (Oral Tumour Patient I, Ncell = 359), and 8.19 ± 5.12 × 105 (Oral Tumour Patient II, Ncell = 175), and were analyzed by statistical approaches including one-way analysis of variance, neural network based pattern recognition and Bayesian estimation, with varied expressions of β-actins among different cell types located. The dataset reported in this study may serve as a reference in future studies of quantitative protein analysis.</div>","PeriodicalId":87330,"journal":{"name":"Nanotechnology and Precision Engineering","volume":"3 3","pages":"Pages 156-161"},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.npe.2020.06.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45896622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Study of material removal behavior on R-plane of sapphire during ultra-precision machining based on modified slip-fracture model 基于改进滑移断裂模型的蓝宝石超精密加工r面材料去除行为研究

Nanotechnology and Precision Engineering Pub Date : 2020-09-01 DOI: 10.1016/j.npe.2020.07.001

Suk Bum Kwon , Aditya Nagaraj , Hae-Sung Yoon , Sangkee Min

引用次数: 4

Theoretical fundamentals of short pulse laser–metal interaction: A review 短脉冲激光与金属相互作用的理论基础综述

Nanotechnology and Precision Engineering Pub Date : 2020-09-01 DOI: 10.1016/j.npe.2020.08.001

Xinxin Li , Yingchun Guan

引用次数: 53

Angle measurement technology based on magnetization vector for narrow space applications 基于磁化矢量的窄空间角度测量技术

Nanotechnology and Precision Engineering Pub Date : 2020-09-01 DOI: 10.1016/j.npe.2020.09.001

Yinguo Huang , Shiyu Zhang , Le Gao , Yelong Zheng

{"title":"Angle measurement technology based on magnetization vector for narrow space applications","authors":"Yinguo Huang , Shiyu Zhang , Le Gao , Yelong Zheng","doi":"10.1016/j.npe.2020.09.001","DOIUrl":"10.1016/j.npe.2020.09.001","url":null,"abstract":"<div>With the wide application of laser in the field of skin plastic surgery, micro laser galvanometer scanner has made great progress in this field with its portability. However, the measurement method used to measure the deflection angle of laser galvanometer in the narrow space of scanner with high precision remains to be studied. In this paper, an angle measurement method based on magnetic field is proposed, and the effect of the shapes of permanent magnets (PMs) on the measurement is studied by theoretical and experimental study under the condition that the maximum available space for the PMs is a 10 mm side cube. An angle measuring experimental device is set up, and the contrast experiment is carried out with different PMs which are the same as simulation. The experimental results show that cylindrical PM is more suitable than other PMs, which is consistent with the simulation results, and the maximum nonlinearity error is 0.562%. This method has the advantages of small volume, non-contact measurement, small moment of inertia, good dynamic response and no external excitation for the PMs, so it has a broad application prospect in micro laser galvanometer scanner.</div>","PeriodicalId":87330,"journal":{"name":"Nanotechnology and Precision Engineering","volume":"3 3","pages":"Pages 167-173"},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.npe.2020.09.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47670059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Optimal design scheme based on the segmental transmission line methodology for high-frequency interconnection in 3D micro/nano integration 基于分段传输线方法的三维微纳集成高频互连优化设计方案

Nanotechnology and Precision Engineering Pub Date : 2020-09-01 DOI: 10.1016/j.npe.2020.09.002

Chenye Li , Zhensong Li , Min Miao

{"title":"Optimal design scheme based on the segmental transmission line methodology for high-frequency interconnection in 3D micro/nano integration","authors":"Chenye Li , Zhensong Li , Min Miao","doi":"10.1016/j.npe.2020.09.002","DOIUrl":"10.1016/j.npe.2020.09.002","url":null,"abstract":"<div>With the continuous growth of signal frequency and package density, discontinuity of the high-frequency interconnection in 3D micro/nano integration becomes unavoidable, which results in serious signal integrity problems. Traditional interconnection design schemes, such as termination and shielding/isolation, cannot meet the requirements under the unified constraints of specific cost, space occupancy, and performance. In this study, a transmission line design optimization scheme based on the segmental transmission line (STL) methodology is proposed. The genetic algorithm is used to select the optimal segment structure parameters of the transmission line to construct an STL with satisfying transmission performance or meet the specific signal amplitude adjustment requirements. This scheme can be adapted to various signal transmission scenarios to significantly improve the signal loss caused by reflection or other negative electromagnetic factors and meet the requirements for the modeling of discontinuous transmission lines. The simulation results show that this scheme is effective in the design scenario of performance improvement or equivalent modeling of discontinuous transmission lines and has significant advantages in circuit area reduction.</div>","PeriodicalId":87330,"journal":{"name":"Nanotechnology and Precision Engineering","volume":"3 3","pages":"Pages 174-186"},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.npe.2020.09.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41409157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A biosensor based on a modified S-taper fiber for target protein detection 一种基于改良s锥纤维的靶蛋白检测传感器

Nanotechnology and Precision Engineering Pub Date : 2020-09-01 DOI: 10.1016/j.npe.2020.08.002

Xiaoqi Liu, Yange Liu, Zhi Wang

引用次数: 3

Sweat detection theory and fluid driven methods: A review 汗液检测理论与流体驱动方法综述

Nanotechnology and Precision Engineering Pub Date : 2020-09-01 DOI: 10.1016/j.npe.2020.08.003

Haixia Yu, Jintao Sun

{"title":"Sweat detection theory and fluid driven methods: A review","authors":"Haixia Yu, Jintao Sun","doi":"10.1016/j.npe.2020.08.003","DOIUrl":"10.1016/j.npe.2020.08.003","url":null,"abstract":"<div>In recent years, analyses of sweat have become more popular since it doesn't require invasive sampling procedures. Although blood still remains the golden standards in clinical, analyses of other common body fluids, such as sweat, have become increasingly important. Because the compositions of sweat and blood are osmotically related, the content of certain metabolites in sweat can directly reflect the disease. Sweat detection can be used as an alternative to blood detection and allows continuous monitoring. Increased development of wearable sensors makes it possible for continuous sweat detection. Here, this paper gave a review about the sweat detection methods, such as fluorescence sensing, electrochemical sensing and colorimetric sensing. The advantages and disadvantages of each method and their developing trend in sweat detection were summarized. Then, for the problem of continuous sweat sampling, three methods (capillary force, hydrogel osmotic pump, evaporation-driven micropump) were introduced through different structures of microfluidic chip, and the level of sweat collection and transport achieved by related research was demonstrated. This review aims to provide guidance for future research in sweat detection and stimulate further interest in continuous monitoring of sweat using microfluidic chip.</div>","PeriodicalId":87330,"journal":{"name":"Nanotechnology and Precision Engineering","volume":"3 3","pages":"Pages 126-140"},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.npe.2020.08.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43162716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Molecular dynamics simulation on the effect of dislocation structures on the retention and distribution of helium ions implanted into silicon 位错结构对注入硅中的氦离子保留和分布影响的分子动力学模拟

Nanotechnology and Precision Engineering Pub Date : 2020-06-01 DOI: 10.1016/j.npe.2020.03.003

Li Ji , Lei Liu , Zongwei Xu , Ying Song , Jintong Wu , Rongrong Li , Fengzhou Fang

引用次数: 4

Study of the material removal mechanism of glass-ceramics based on consecutive incremental loading in ductile-regime grinding 基于延性磨削连续增量加载的微晶玻璃材料去除机理研究

Nanotechnology and Precision Engineering Pub Date : 2020-06-01 DOI: 10.1016/j.npe.2020.02.002

Xue Li

{"title":"Study of the material removal mechanism of glass-ceramics based on consecutive incremental loading in ductile-regime grinding","authors":"Xue Li","doi":"10.1016/j.npe.2020.02.002","DOIUrl":"10.1016/j.npe.2020.02.002","url":null,"abstract":"<div>Glass-ceramics have many excellent properties and are widely used in various fields. During the grinding process, the workpiece surface is typically subject to material removal by grit of incremental heights, which has rarely been the focus of research. As such, it is necessary to study the material removal mechanism of glass-ceramics under consecutive incremental loading, which more closely reflects the actual grinding process. In this paper, to analyze the plastic deformation and residual stress of lithium aluminosilicate (LAS) glass-ceramics, a finite element model is established based on the Drucker–Prager yield criterion for ductile regimes. A nano-scratch test was also conducted and the test results show that both the residual depth and residual stress increase with an increase in the number of increments, and that consecutive incremental loading promotes the plastic deformation of glass-ceramics and increases the residual stress of the material in the ductile-regime process. These findings provide guidance for achieving higher dimensional accuracy in the actual grinding of glass-ceramics parts.</div>","PeriodicalId":87330,"journal":{"name":"Nanotechnology and Precision Engineering","volume":"3 2","pages":"Pages 88-95"},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.npe.2020.02.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42415975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

An interdigitated impedance microsensor for detection of moisture content in engine oil 一种用于检测发动机油中水分含量的交叉数字阻抗微传感器

Nanotechnology and Precision Engineering Pub Date : 2020-06-01 DOI: 10.1016/j.npe.2020.04.001

Haitao Liu , Xiecheng Tang , Hao Lu , Wenguang Xie , Yanmin Hu , Qiannan Xue

引用次数: 13