Microphysiological systems最新文献

Erratum to Body-in-a-Cube: a microphysiological system for multi-tissue co-culture with near-physiological amounts of blood surrogate 立方体中的身体勘误表：一个用于与接近生理量的血液替代物进行多组织共培养的微物理系统

Microphysiological systems Pub Date : 2021-06-01 DOI: 10.21037/mps-2021-1

Editorial Office

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引用次数: 1

Microphysiological systems Pub Date : 2021-01-01 Epub Date: 2021-01-26 DOI: 10.21037/mps-20-6

Lucas Humayun, Colin Smith, Wanlu Li, Yu Shrike Zhang, Christine Park, Wuwei Feng, Junjie Yao

引用次数: 0

Recent advancements of human iPSC derived cardiomyocytes in drug screening and tissue regeneration 人iPSC来源的心肌细胞在药物筛选和组织再生中的最新进展

Microphysiological systems Pub Date : 2020-09-01 DOI: 10.21037/MPS-20-3

Yike Huang, Ting-zhong Wang, M. López, Minoru Hirano, A. Hasan, S. Shin

{"title":"Recent advancements of human iPSC derived cardiomyocytes in drug screening and tissue regeneration","authors":"Yike Huang, Ting-zhong Wang, M. López, Minoru Hirano, A. Hasan, S. Shin","doi":"10.21037/MPS-20-3","DOIUrl":"https://doi.org/10.21037/MPS-20-3","url":null,"abstract":": Myocardial infarction together with subsequent heart failures are among the main reasons for death related to cardiovascular diseases (CVD). Restoring cardiac function and replacing scar tissue with healthy regenerated cardiomyocytes (CMs) is a hopeful therapy for heart failure. Human-induced pluripotent stem cell (hiPSC) derived CMs (hiPSC-CMs) offer the advantages of not having significant ethical issues and having negligible immunological rejection compared to other myocardial regeneration methods. hiPSCs can also produce an unlimited number of human CMs, another advantage they have compared with other cell sources for cardiac regeneration. Numerous researchers have focused their work on promoting the functional maturity of hiPSC-CMs, as well as finding out the precise regulatory mechanisms of each differentiation stage together with the economical and practical methods of acquisition and purification. However, the clinical applications of hiPSC-CMs in drug discovery and cardiac regeneration therapy have yet to be achieved. In this review, we present an overview of various methods for improving the differentiation efficiency of hiPSC-CMs and discuss the differences of electrophysiological characteristics between hiPSC-CMs and matured native CMs. We also introduce approaches for obtaining a large quantity of iPSC-CMs, which are needed to achieve biomanufacturing strategies for building biomimetic three-dimensional tissue constructs using combinations of biomaterials and advanced microfabrication techniques. Recent advances in specific iPSC technology-based drug screening platforms and regeneration therapies can suggest future directions for personalized medicine in biomedical applications. 22","PeriodicalId":87327,"journal":{"name":"Microphysiological systems","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42039766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Body-in-a-Cube: a microphysiological system for multi-tissue co-culture with near-physiological amounts of blood surrogate. 立方体中的身体：一种利用接近生理量的血液替代物进行多组织共培养的微观生理系统。

Microphysiological systems Pub Date : 2020-06-01 DOI: 10.21037/mps-19-8

Longyi Chen, Yang Yang, Hidetaka Ueno, Mandy B Esch

{"title":"Body-in-a-Cube: a microphysiological system for multi-tissue co-culture with near-physiological amounts of blood surrogate.","authors":"Longyi Chen, Yang Yang, Hidetaka Ueno, Mandy B Esch","doi":"10.21037/mps-19-8","DOIUrl":"10.21037/mps-19-8","url":null,"abstract":"Background: Decreasing the amount of liquid inside microphysiological systems (MPS) can help uncover the presence of toxic drug metabolites. However, maintaining near-physiological volume ratios among blood surrogate and multiple organ mimics is technically challenging. Here, we developed a body cube and tested its ability to support four human tissues (kidney, GI tract, liver, and bone marrow) scaled down from in vivo functional volumes by a factor of 73,000 with 80 μL of cell culture medium (corresponding to ~1/73000th of in vivo blood volume).Methods: GI tract cells (Caco-2), liver cells (HepG2/C3A), bone marrow cells (Meg-01), and kidney cells (HK-2) were co-cultured inside the body cube with 80 μL of common, recirculating cell culture medium for 72 h. The system was challenged with acetaminophen and troglitazone, and concentrations of aspartate aminotransferase (AST), albumin, and urea were monitored over time.Results: Cell viability analysis showed that 95.5%±3.2% of liver cells, 89.8%±4.7% of bone marrow cells, 82.8%±8.1% of GI tract cells, and 80.1%±11.5% of kidney cells were viable in co-culture for 72 h. Both acetaminophen and troglitazone significantly lowered cell viability in the liver chamber as indicated by viability analysis and a temporary increase of AST in the cell culture medium. Both drugs also lowered urea production in the liver by up to 45%.Conclusions: Cell viability data and the production of urea and albumin indicate that the co-culture of GI tract, liver, bone marrow, and kidney tissues with near-physiological volume ratios of tissues to blood surrogate is possible for up to 72 h. The body-cube was capable of reproducing liver toxicity to HepG2/C3A liver cells via acetaminophen and troglitazone. The developed design provides a viable format for acute toxicity testing with near-physiological blood surrogate to tissue volume ratios.","PeriodicalId":87327,"journal":{"name":"Microphysiological systems","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/67/4c/nihms-1670586.PMC8201523.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39240528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering bone marrow-on-a-chip 芯片工程骨髓

Microphysiological systems Pub Date : 2019-12-03 DOI: 10.21037/MPS.2019.02.01

Y. Torisawa

引用次数: 2

Fiber-based microphysiological systems: a powerful tool for high throughput drug screening 基于纤维的微生理系统:高通量药物筛选的有力工具

Microphysiological systems Pub Date : 2019-09-17 DOI: 10.21037/mps.2019.08.01

Tavia Walsh, Lucas Karperien, S. M. H. Dabiri, M. Akbari

{"title":"Fiber-based microphysiological systems: a powerful tool for high throughput drug screening","authors":"Tavia Walsh, Lucas Karperien, S. M. H. Dabiri, M. Akbari","doi":"10.21037/mps.2019.08.01","DOIUrl":"https://doi.org/10.21037/mps.2019.08.01","url":null,"abstract":"The growing demand for improvements in in vitro drug screening, disease modelling, and regenerative tissue therapies poses an urgent clinical need for the continued development of innovative tissue engineering strategies. Several promising biofabrication and aggregation solutions have been developed to overcome these challenges, such as three-dimensional (3D) bioprinting and chip-based physiological models. However, replicating the structural and cytoarchitectural complexities of native tissue types to achieve biomimicry of epithelial, nervous, muscular, and connective tissues remains a significant area of investigation. Innovative research efforts focused on the thread-like morphologies of bodily tissues have been developed to overcome these challenges. This review features the recent and current work done in fiber fabrication methods and the associated textile assembly techniques utilized to create fiber-based microphysiological systems, specifically for applications in disease modelling and high throughput drug testing. The advantages and disadvantages of these different fiber fabrication and assembly approaches are compared. Finally, the applications of these textile-based biofabrication approaches in epithelial, nervous, connective, and muscle tissue engineering are reviewed.","PeriodicalId":87327,"journal":{"name":"Microphysiological systems","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21037/mps.2019.08.01","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42667375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Organs-on-chips: latest developments 芯片上的器官：最新进展

Microphysiological systems Pub Date : 2019-01-04 DOI: 10.21037/MPS.2018.12.01

A. D. Meer

引用次数: 0

Microphysiological systems to study microenvironment-cell nucleus interaction: importance of tissue geometry and heterogeneity 微生理系统研究微环境-细胞核相互作用:组织几何和异质性的重要性

Microphysiological systems Pub Date : 2018-11-12 DOI: 10.21037/mps.2018.11.02

S. Lelièvre, Shirisha Chittiboyina

{"title":"Microphysiological systems to study microenvironment-cell nucleus interaction: importance of tissue geometry and heterogeneity","authors":"S. Lelièvre, Shirisha Chittiboyina","doi":"10.21037/mps.2018.11.02","DOIUrl":"https://doi.org/10.21037/mps.2018.11.02","url":null,"abstract":"Theart of three-dimensional (3D) cell culture is to place cells in an environmentthat facilitates their differentiation into physiologically relevant tissues.The mammary gland was the original model for the development of 3D cellculture. With simple microphysiological systems for this model relying on thepresence of extracellular matrix (ECM) components, we initially showed thatnormal differentiation, illustrated by the formation of a basoapical polarityaxis and cell quiescence, relied on a specific organization of the cellnucleus, including the epigenome, itself under the control of the ECMmicroenvironment. To further explore microenvironment-nucleus interaction, werecently developed two organ-on-a-chip systems. The disease-on-a-chip was used tostudy mechanical influence by creating a model of ductal environment made ofcarved hemichannels. It revealed that the curvature of hemichannels directsnuclear morphometry to such an extent that tumors growing inside this geometry(as they do in vivo ) display distinct sensitivity to anticancer drugs.These findings shed light on previous observation that the expression ofproteins involved in anticancer drug response correlate with nuclearmorphometry and highlight the importance of choosing the appropriate 3D cellculture system for in vitro preclinical screening of drugs. Thegradient-on-a-chip was used to create, via microfluidics, a range ofextracellular concentrations of soluble components within the same culturechamber. With this system, it became apparent that oxidizing molecules presentin the ECM of cancers and suspected to contribute to progression and resistanceto treatment, have an influence on nuclear morphometric features recognized asa sign of aggressiveness; however, oxidizer concentration-mediated phenotypicswitch depends on ECM stiffness. These findings suggest that tissueheterogeneity characterizing cancers is directed by competitive and synergisticinfluences among extracellular factors controlling cell phenotype via an impacton the cell nucleus.","PeriodicalId":87327,"journal":{"name":"Microphysiological systems","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45005305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Microphysiological systems: analysis of the current status, challenges and commercial future. 微生理系统:现状、挑战及商业前景分析。

Microphysiological systems Pub Date : 2018-11-05 eCollection Date: 2018-11-01 DOI: 10.21037/mps.2018.10.01

João Ribas, Joanna Pawlikowska, Jeroen Rouwkema

引用次数: 14

3D bioprinting for modelling vasculature. 用于血管系统建模的3D生物打印。

Microphysiological systems Pub Date : 2018-11-01 Epub Date: 2018-11-05 DOI: 10.21037/mps.2018.10.02

Pranabesh Sasmal, Pallab Datta, Yang Wu, Ibrahim T Ozbolat

引用次数: 46