Basic and Clinical Andrology最新文献

{"title":"Correction: Complete or partial loss of the Y chromosome in an unselected cohort of 865 non-vasectomized, azoospermic men","authors":"J. Fedder, C. Fagerberg, M. W. Jørgensen, C. H. Gravholt, A. Berglund, U. B. Knudsen, A. Skakkebæk","doi":"10.1186/s12610-023-00218-7","DOIUrl":"https://doi.org/10.1186/s12610-023-00218-7","url":null,"abstract":"<p><b>Correction: Basic Clin. Androl. 33, 37 (2023)</b></p><p><b>https://doi.org/10.1186/s12610-023-00212-z</b></p><p>Following publication of the original article [1], the authors reported a typesetting error in the <b>Discussion</b> section. The heading <b>45,X and 46,XX males</b> was mistakenly written as X45,X and 46,XX males. The publishers apologize for this typesetting error.</p><p>The original article [1] has been corrected.</p><ol data-track-component=\"outbound reference\"><li data-counter=\"1.\"><p>Fedder J, Fagerberg C, Jørgensen M, et al. Complete or partial loss of the Y chromosome in an unselected cohort of 865 non-vasectomized, azoospermic men. Basic Clin Androl. 2023;33:37. https://doi.org/10.1186/s12610-023-00212-z.</p><p>Article CAS PubMed PubMed Central Google Scholar </p></li></ol><p>Download references<svg aria-hidden=\"true\" focusable=\"false\" height=\"16\" role=\"img\" width=\"16\"><use xlink:href=\"#icon-eds-i-download-medium\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"></use></svg></p><h3>Authors and Affiliations</h3><ol><li><p>Centre of Andrology &amp; Fertility Clinic, Odense University Hospital, Kløvervænget 23, 5000, Odense, Denmark</p><p>J. Fedder</p></li><li><p>Department of Clinical Medicine, University of Southern Denmark, Odense, Denmark</p><p>J. Fedder</p></li><li><p>Fertility Clinic, Horsens Hospital, Horsens, Denmark</p><p>J. Fedder &amp; U. B. Knudsen</p></li><li><p>Department of Clinical Genetics, Odense University Hospital, Odense, Denmark</p><p>C. Fagerberg &amp; A. Berglund</p></li><li><p>Department of Clinical Genetics, Lillebaelt Hospital, Vejle, Denmark</p><p>M. W. Jørgensen</p></li><li><p>Department of Molecular Medicine, Aarhus University Hospital, Aarhus, Denmark</p><p>C. H. Gravholt, A. Berglund &amp; A. Skakkebæk</p></li><li><p>Department of Endocrinology, Aarhus University Hospital, Aarhus, Denmark</p><p>C. H. Gravholt</p></li><li><p>Department of Clinical Medicine, Aarhus University, Aarhus, Denmark</p><p>C. H. Gravholt, U. B. Knudsen &amp; A. Skakkebæk</p></li><li><p>Department of Clinical Genetics, Aarhus University Hospital, Aarhus, Denmark</p><p>A. Berglund &amp; A. Skakkebæk</p></li></ol><span>Authors</span><ol><li><span>J. Fedder</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>C. Fagerberg</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>M. W. Jørgensen</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>C. H. Gravholt</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>A. Berglund</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>U. B. Knudsen</span>View author publicati","PeriodicalId":8730,"journal":{"name":"Basic and Clinical Andrology","volume":"52 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139102394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of acupuncture for psychogenic erectile dysfunction: a randomized, sham-controlled trial","authors":"Hao Wang, Xulu Lei, Dongyue Ma, Ziwei Zhao, Anmin Wang, Guanchao Du, Jiwei Zhang, Fu Wang, Jun Guo","doi":"10.1186/s12610-023-00215-w","DOIUrl":"https://doi.org/10.1186/s12610-023-00215-w","url":null,"abstract":"Psychogenic erectile dysfunction (pED) is a common sexual dysfunction often accompanied by psychosomatic factors. Its treatment includes oral medications, psychotherapy, and behavioral therapy. Acupuncture’s effect on erectile function in pED patients remains to be investigated. This randomized study evaluated the effects of acupuncture and sham acupuncture in pED patients. Altogether, 66 men with pED were randomized to the acupuncture (n = 33) or sham acupuncture group (n = 33). Both groups have a 6-week treatment with 18 sessions. Primary outcome was the International Index of Erectile Function-5 (IIEF-5) at 6 weeks. Secondary outcomes were IIEF-5 (weeks 2, 4, and 10), erection hardness score (EHS), sexual encounter profile-2 (SEP-2), SEP-3, self-rating anxiety scale (SAS), and self-rating depression scale (SDS). Among the 66 participants, 64 completed the outcome measurements at week 10. Both acupuncture and sham acupuncture groups had improved IIEF-5 and EHS and decreased SAS and SDS post-treatment (p < 0.05). The acupuncture group had significantly better improvement in IIEF-5, EHS, and SEP-3 and significantly reduced SAS and SDS than the sham acupuncture group (p < 0.05). The improvement in SEP-2 post-treatment was not significantly different between the two groups (p > 0.05). There were no serious adverse events. The 6-week acupuncture treatment significantly improved the erectile capacity and psychosomatic status of pED patients. ChiCTR2200064345 (Chinese Clinical Trial Registry) ( https://www.chictr.org.cn/showproj.html?proj=174873 ).","PeriodicalId":8730,"journal":{"name":"Basic and Clinical Andrology","volume":"28 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138826205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nrf2 enhances the therapeutic efficiency of adipose-derived stem cells in the treatment of neurogenic erectile dysfunction in a rat model.","authors":"Shangbin Yang, Wancheng Shi, Qianhui Liu, Yingqiu Song, Jiafeng Fang","doi":"10.1186/s12610-023-00214-x","DOIUrl":"10.1186/s12610-023-00214-x","url":null,"abstract":"<p><strong>Background: </strong>Erectile dysfunction (ED) caused by intraoperative nerve injury is a major complication of pelvic surgery. Adipose-derived stem cells (ADSCs) have presented therapeutic potential in a rat model of bilateral cavernous nerve injury (BCNI), while inadequate in vivo viability has largely limited their application. Nuclear factor-E2-related Factor (Nrf2) is a key transcription factor that regulates cellular anti-oxidative stress. In this work, we investigated the effect of Nrf2 expression regulation on the viability of ADSCs, and explore its repair potential in a BCNI rat model.</p><p><strong>Results: </strong>The survival time of tert-Butylhydroquinone (tBHQ)-ADSCs in BCNI model increased obviously. In addition, the tBHQ-ADSCs group presented better restoration of major pelvic ganglion (MPG) nerve contents and fibers, better improvement of erectile function, and less penile fibrosis than the other groups. Moreover, the expression of Nrf2 and superoxide dismutase 1 (SOD1) were higher than those of other groups.</p><p><strong>Conclusion: </strong>Nrf2 could enhance the anti-oxidative stress ability of ADSCs, so as to improve the therapeutic effect of ADSCs on BCNI rat model.</p>","PeriodicalId":8730,"journal":{"name":"Basic and Clinical Andrology","volume":"33 1","pages":"39"},"PeriodicalIF":2.4,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10731878/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138796267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Which inflammatory marker, between systemic immune-inflammation index and neutrophil to eosinophil ratio, is associated with Peyronie’s disease and are there any implications for a better understanding of its mechanisms?","authors":"Felice Crocetto, Ciro Imbimbo, Biagio Barone, Davide Turchino, Umberto Marcello Bracale, Antonio Peluso, Marco Panagrosso, Alfonso Falcone, Benito Fabio Mirto, Luigi De Luca, Enrico Sicignano, Francesco Del Giudice, Gian Maria Busetto, Giuseppe Lucarelli, Gaetano Giampaglia, Celeste Manfredi, Matteo Ferro, Giovanni Tarantino","doi":"10.1186/s12610-023-00213-y","DOIUrl":"https://doi.org/10.1186/s12610-023-00213-y","url":null,"abstract":"Peyronie’s disease affects up to 9% of men and is often accompanied by pain and/or erectile dysfunction. It is characterized by an inflammatory process that is the grassroots of the subsequent fibrosis stage. There is an unmet need to evaluate its onset and progression. Among the newly proposed biomarkers of inflammation, authors developed a novel systemic immune-inflammation index (SII) based on lymphocyte, neutrophil, and platelet counts. Similarly, a recent study reported that a neutrophil-to-eosinophil ratio (NER) represents systemic inflammation. A 49-patient group with Peyronie’s disease as confronted with 50 well-matched for age and BMI controls. As laboratory evaluation of inflammation, SII, NER and the eosinophil to neutrophil ratio (ENR) were studied. As a likely risk factor for the presence of Peyronie’s disease, a higher prevalence of hypercholesterolemia, hyperglycemia and hypertension was discovered in the patients compared to controls. A significant difference was found in the median values of the NER between the two selected groups, i.e., 32.5 versus 17.3 (p = 0.0021). As expected, also ENR was significantly different. The receiver operating characteristic curves for SII, ENR and NER were 0.55, 0.32 and 0.67, respectively, highlighting the best performance of NER. The cut-off for NER was 12.1, according to the Youden test. According to our results, any evaluation of circulating eosinophil, evaluated as NER, beyond being a signature of immuno-inflammatory response, help assess tissue homeostasis, since eosinophils are now considered multifunctional leukocytes and give a picture of the inflammatory process and repair process belonging to Peyronie’s disease.","PeriodicalId":8730,"journal":{"name":"Basic and Clinical Andrology","volume":"27 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138743878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Mechanism of Shugan Yidan fang, a Chinese herbal formula, in rat model of premature ejaculation","authors":"Qiang Han, Jun Guo, Renyuan Wang, Jiangminzi Li, Fu Wang, Qinghe Gao, Jiwei Zhang, Hetian Wang, Yin Zeng","doi":"10.1186/s12610-023-00216-9","DOIUrl":"https://doi.org/10.1186/s12610-023-00216-9","url":null,"abstract":"<p><b>Correction: Basic Clin Androl 33, 25 (2023)</b></p><p><b>https://doi.org/10.1186/s12610-023-00200-3</b></p><p>Following publication of the original article [1], the authors reported an error in the title of the article. The original title “Mechanism of Shugan Yidan fan, a Chinese herbal formula, in rat model of premature ejaculation” should be replaced with “Mechanism of Shugan Yidan fang, a Chinese herbal formula, in rat model of premature ejaculation”. Principally, it should be Shugan Yidan fang, not Shugan Yidan fan.</p><p>We sincerely apologize for the error.</p><p>The original article [1] has been corrected.</p><ol data-track-component=\"outbound reference\"><li data-counter=\"1.\"><p>Han Q, Guo J, Wang R, et al. Mechanism of Shugan Yidan fang, a Chinese herbal formula, in rat model of premature ejaculation. Basic Clin Androl. 2023;33:25. https://doi.org/10.1186/s12610-023-00200-3.</p><p>Article PubMed PubMed Central Google Scholar </p></li></ol><p>Download references<svg aria-hidden=\"true\" focusable=\"false\" height=\"16\" role=\"img\" width=\"16\"><use xlink:href=\"#icon-eds-i-download-medium\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"></use></svg></p><h3>Authors and Affiliations</h3><ol><li><p>Department of Andrology, Dongcheng District, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, 23 Art Gallery Back Street, Beijing, China</p><p>Qiang Han, Renyuan Wang, Hetian Wang &amp; Yin Zeng</p></li><li><p>Department of Andrology, Xiyuan Hospital of China Academy of Traditional Chinese Medicine, Beijing, 100089, China</p><p>Jun Guo, Fu Wang, Qinghe Gao &amp; Jiwei Zhang</p></li><li><p>Department of Endocrinology, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing, 100010, China</p><p>Jiangminzi Li</p></li></ol><span>Authors</span><ol><li><span>Qiang Han</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Jun Guo</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Renyuan Wang</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Jiangminzi Li</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Fu Wang</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Qinghe Gao</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Jiwei Zhang</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Hetian Wang</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</","PeriodicalId":8730,"journal":{"name":"Basic and Clinical Andrology","volume":"98 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138715217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complete or partial loss of the Y chromosome in an unselected cohort of 865 non-vasectomized, azoospermic men","authors":"J Fedder, C Fagerberg, MW Jørgensen, CH Gravholt, A Berglund, UB Knudsen, A Skakkebæk","doi":"10.1186/s12610-023-00212-z","DOIUrl":"https://doi.org/10.1186/s12610-023-00212-z","url":null,"abstract":"Structural abnormalities as well as minor variations of the Y chromosome may cause disorders of sex differentiation or, more frequently, azoospermia. This study aimed to determine the prevalence of loss of Y chromosome material within the spectrum ranging from small microdeletions in the azoospermia factor region (AZF) to complete loss of the Y chromosome in azoospermic men. Eleven of 865 azoospermic men (1.3%) collected from 1997 to 2022 were found to have a karyotype including a 45,X cell line. Two had a pure 45,X karyotype and nine had a 45,X/46,XY mosaic karyotype. The AZF region, or part of it, was deleted in eight of the nine men with a structural abnormal Y-chromosome. Seven men had a karyotype with a structural abnormal Y chromosome in a non-mosaic form. In addition, Y chromosome microdeletions were found in 34 men with a structural normal Y chromosome. No congenital malformations were detected by echocardiography and ultrasonography of the kidneys of the 11 men with a 45,X mosaic or non-mosaic cell line. In men with azoospermia, Y chromosome loss ranging from small microdeletions to complete loss of the Y chromosome was found in 6.1% (53/865). Partial AZFb microdeletions may give a milder testicular phenotype compared to complete AZFb microdeletions.","PeriodicalId":8730,"journal":{"name":"Basic and Clinical Andrology","volume":"85 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138630207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient pathway for men fertility preservation in testicular cancer or lymphoma: a cross-sectional study of national 2018 data","authors":"Ségolène Prades, Sarah-Lyne Jos, Jacqueline Saïas-Magnan, Louis Bujan, Florence Eustache, Oxana Blagosklonov, Eric Lechevallier, Florence Brugnon, Vanessa Loup-Cabaniols, Dorian Bosquet, Marie Prades, Bérengère Ducrocq, Céline Chalas, Sandrine Giscard-d’Estaing, Anne Mayeur, Isabelle Koscinsky, Françoise Schmitt, Aline Papaxanthos-Roche, Marius Teletin, Emmanuelle Thibault, Damien Beauvillard, Sophie Mirallie, Béatrice Delepine, Annie Benhaim, Pascale May-Panloup, Ségolène Veau, Cynthia Frapsauce, Patricia Fauque, Régis Costello, Nathalie Rives, Catherine Metzler-Guillemain, Jeanne Perrin","doi":"10.1186/s12610-023-00209-8","DOIUrl":"https://doi.org/10.1186/s12610-023-00209-8","url":null,"abstract":"In 15–49 years-old men, the main cancers are testicular cancer (TC) and lymphomas (L): freezing of ejaculated sperm is primarily used for male fertility preservation (FP) before cancer treatment. Our objective was to analyze the French FP rate in 15–49 years-old men diagnosed with TC or L in 2018. We designed a national descriptive cross-sectional study of sperm banking rate in men with a diagnosis of TC, Hodgkin L (HL) or non-Hodgkin L (NHL). From the French National Cancer Institute (INCa) 2018 data, we extracted the estimated incidence of TC and L in metropolitan France. From the 2018 activity report of CECOS network (Centers for Study and Banking of Eggs and Sperm), we extracted the number of men with TC or L who banked ejaculated sperm. We estimated the proportion of 15–49 years-old men diagnosed with TC or L who banked sperm. Among 15–49 years-old men, INCa estimated 38,048 new cancer diagnoses in metropolitan France in 2018: 2,630 TC and 3,913 L (943 HL and 2,970 NHL). The CECOS network provided data from 26/27 metropolitan centers (96% response rate): 1,079 sperm banking for men with TC, 375 for HL and 211 for NHL. We estimated that the 2018 sperm banking rate in France was 41% for TC, 40% for HL, and 7% for NHL. To our knowledge, our paper is the first cross-sectional study with multicenter and national data analyzing FP rate in cancer men: it suggests an efficient pathway for men to FP before cancer treatment, compared to previously published studies. Although sperm banking rate in 15–49 years-old men could definitely be improved, further studies should evaluate the information given to patients before gonadotoxic treatments, the factors associated with the absence of sperm banking and whether this lack of referral induces a loss of chance for these men.","PeriodicalId":8730,"journal":{"name":"Basic and Clinical Andrology","volume":"20 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138577075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Daily intake of 30 mg duloxetine is effective in decreasing premature ejaculation severity: a prospective randomized placebo-controlled cross over clinical trial.","authors":"Adham Zaazaa, Mohamed Nasr Eldin, Sameh Fayek GamalEl Din, Ashraf Zeidan, Mohamed Yassin Mohamed Saleh, Ahmed Adel, Mohamed Shokr","doi":"10.1186/s12610-023-00210-1","DOIUrl":"10.1186/s12610-023-00210-1","url":null,"abstract":"<p><strong>Background: </strong>Premature ejaculation (PE) is considered to be the most common male sexual disorder affecting 20% to 66% of sexually active men. Most of the patients had already tried on demand dapoxitine with no improvement. We aimed in the current study to assert the efficacy and safety profile of daily intake of 30 mg duloxetine in treating patients with lifelong premature ejaculation (LPE) as well as patients with acquired premature ejaculation (APE).</p><p><strong>Results: </strong>The current study showed significant improvement in intravaginal ejaculatory latency time (IELT) after intake of duloxetine. All participants had a median Arabic index of premature ejaculation (AIPE) of 26, median IELT of 180 s, median male sexual quality of life (SQOL) of 43 after being treated with duloxetine (p value < 0.001 for all). While median AIPE after placebo was 19, median IELT after placebo was 60 s and median male SQOL after placebo was 21. Paired comparison of AIPE, IELT (Secs), inter quartile range (IQR) and male SQOL in group (A) patients at baseline and after duloxetine intake showed statistically significant improvement among treated patients (p values < 0.001 for all). Paired comparison of AIPE, IELT (Secs), IQR and male SQOL in group (A) patients at baseline and after placebo treatment showed no significant improvement of male SQOL. Furthermore, AIPE and IELT returned to baseline scores after discontinuation of duloxetine (p values 0.729; 0.892, respectively). Paired comparison of AIPE, IELT (Secs), IQR and male SQOL in group (B) patients at baseline and after placebo treatment showed almost same scores of patients in group (A) who received placebo for 2 months after a 2 month washout period (p values 1.000 for all). Paired comparison of AIPE, IELT (Secs), IQR and male SQOL in group (B) patients at baseline and after duloxetine treatment showed statistically significant improvement among all treated patients (p values < 0.001 for all).</p><p><strong>Conclusion: </strong>Duloxetine is an effective drug for treatment of LPE and APE patients. Further, larger studies are needed to compare duloxetine to different known therapeutic modalities for PE to assert it's efficacy and superiority.</p>","PeriodicalId":8730,"journal":{"name":"Basic and Clinical Andrology","volume":"33 1","pages":"34"},"PeriodicalIF":2.4,"publicationDate":"2023-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10696770/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138481869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sperm chromatin structure assay (SCSA^®) and flow cytometry-assisted TUNEL assay provide a concordant assessment of sperm DNA fragmentation as a function of age in a large cohort of approximately 10,000 patients.","authors":"Paria Behdarvandian, Ali Nasr-Esfahani, Marziyeh Tavalaee, Kosar Pashaei, Nushin Naderi, Zahra Darmishonnejad, Jorge Hallak, Robert J Aitken, Parviz Gharagozloo, Joël R Drevet, Mohammad Hossein Nasr-Esfahani","doi":"10.1186/s12610-023-00208-9","DOIUrl":"https://doi.org/10.1186/s12610-023-00208-9","url":null,"abstract":"<p><strong>Background: </strong>Sperm DNA integrity is increasingly seen as a critical characteristic determining reproductive success, both in natural reproduction and in assisted reproductive technologies (ART). Despite this awareness, sperm DNA and nuclear integrity tests are still not part of routine examinations for either infertile men or fertile men wishing to assess their reproductive capacity. This is not due to the unavailability of DNA and sperm nuclear integrity tests. On the contrary, several relevant but distinct tests are available and have been used in many clinical trials, which has led to conflicting results and confusion. The reasons for this are mainly the lack of standardization between different clinics and between the tests themselves. In addition, the small number of samples analyzed in these trials has often weakened the value of the analyses performed. In the present work, we used a large cohort of semen samples, covering a wide age range, which were simultaneously evaluated for sperm DNA fragmentation (SDF) using two of the most frequently used SDF assays, namely the TUNEL assay and the sperm chromatin structure assay (SCSA®). At the same time, as standard seminal parameters (sperm motility, sperm morphology, sperm count) were available for these samples, correlations between age, SDF and conventional seminal parameters were analyzed.</p><p><strong>Results: </strong>We show that the SCSA® and TUNEL assessments of SDF produce concordant data. However, the SDF assessed by TUNEL is systematically lower than that assessed by SCSA®. Regardless of the test used, the SDF increases steadily during aging, while the HDS parameter (High DNA stainability assessed via SCSA®) remains unchanged. In the cohort analyzed, conventional sperm parameters do not seem to discriminate with aging. Only sperm volume and motility were significantly lower in the oldest age group analyzed [50-59 years of age].</p><p><strong>Conclusions: </strong>In the large cohort analyzed, SDF is an age-dependent parameter, increasing linearly with aging. The SCSA® assessment of SDF and the flow cytometry-assisted TUNEL assessment are well correlated, although TUNEL is less sensitive than SCSA®. This difference in sensitivity should be taken into account in the final assessment of the true level of fragmentation of the sperm nucleus of a given sample. The classical sperm parameters (motility, morphology, sperm count) do not change dramatically with age, making them inadequate to assess the fertility potential of an individual.</p>","PeriodicalId":8730,"journal":{"name":"Basic and Clinical Andrology","volume":"33 1","pages":"33"},"PeriodicalIF":2.4,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10688019/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138457555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"External quality assessment scheme for sperm DNA fragmentation: a pilot study in China.","authors":"Yan Zheng, Ying-Bi Wu, Ye-Lin Jia, Li-Juan Ying, Ting-Ting Yang, Qing-Yuan Cheng, Jiao Qin, Chen Luo, Lin Yu, Fu-Ping Li","doi":"10.1186/s12610-023-00211-0","DOIUrl":"10.1186/s12610-023-00211-0","url":null,"abstract":"<p><strong>Background: </strong>The aim of this article is to establish an external quality assessment (EQA) scheme for sperm Deoxyribonucleic acid (DNA) fragmentation (SDF) detection, and to assess the feasibility of the scheme. In addition, this article provides some case analysis of abnormal results in order to really help improve the performance of the laboratory.</p><p><strong>Results: </strong>In 2021 and 2022, 10 and 28 laboratories in China volunteered to participate in the EQA program respectively. Two samples were selected for EQA each year, a large spread of results was obtained for the four samples, and the highest values were 13.7, 4.2, 8.0 and 4.0 times the lowest respectively. The coefficients of variation (CVs) were very high for the four samples, at 46.6%, 30.1%, 26.7% and 30.3%, respectively. The CVs of the samples with high SDF values were lower than those of the samples with low SDF values. There was no significant difference between the results of sperm chromatin structure assay (SCSA) and sperm chromatin dispersion (SCD). For the 10 laboratories that participated in EQA in 2021 and 2022, the CVs of low SDF value samples and high SDF value samples decreased from 46.6% and 30.1% in 2021 to 32.5% and 22.7% in 2022, respectively.</p><p><strong>Conclusion: </strong>This is the first study to evaluate the EQA program on SDF, which involved a number of laboratories and was demonstrated to be feasible. It is recommended that all laboratories participate in the EQA of SDF to ensure the accuracy of the results.</p>","PeriodicalId":8730,"journal":{"name":"Basic and Clinical Andrology","volume":"33 1","pages":"36"},"PeriodicalIF":2.4,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10683090/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138443623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}