Blood and genomics最新文献_第3页

Carbonic anhydrases: hematologic relevance and a biosensing perspective 碳酸酐酶:血液学相关性和生物传感的观点

Blood and genomics Pub Date : 2020-01-01 DOI: 10.46701/bg.2020012019132

M. Gheorghiu

引用次数: 0

Electrical impedance assays of blood cells 血细胞的电阻抗测定

Blood and genomics Pub Date : 2020-01-01 DOI: 10.46701/bg.2020012019123

E. Gheorghiu

{"title":"Electrical impedance assays of blood cells","authors":"E. Gheorghiu","doi":"10.46701/bg.2020012019123","DOIUrl":"https://doi.org/10.46701/bg.2020012019123","url":null,"abstract":"In this review, the capability of electrical impedance spectroscopy analysis of blood cells, especially for red blood cells is presented, highlighting its large area of related biomedical relevance. The method is briefly intro-duced and basic theoretical aspects are discussed by considering both phenomenological (e.g. equivalent circuit) and microscopic approaches. The latter include a comparative analysis of the relevance of considering real shape (consistent with microscopic observations) versus spheroidal approximations (prolate and oblate spheroids) with the same surface and volume concentration. We show that while ellipsoidal approximation is fairly good for randomly oriented cells, it is quite poor whenever oriented cells are measured. The voluminous literature on the electrical analysis of blood cells is reviewed to stress the most promising biomedical applications of the method either per se or in combination with complementary e.g. (micro) fluidic approaches. blood sample under test (SUT), geometric factor of the SUT(GESUT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), cardiovascular diseases (CVDs), prothrombin time (PT), partial thromboplastin time (PTT), and thrombin clotting time (TCT), electric impedance microflow cytometry (EIMC), sickle cell disease (SCD), acquired immunodeficiency syndrome (AIDS).","PeriodicalId":87226,"journal":{"name":"Blood and genomics","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81966771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Clinical features and hematological findings of COVID-19 patients with blood transfusion COVID-19输血患者的临床特点及血液学表现

Blood and genomics Pub Date : 2020-01-01 DOI: 10.46701/bg.2020022020125

Yanhong Zhang, Shangen Zheng, Qiangqiang Zhou, Yu Zhang, Yao Zheng, Shun Wang, Lei Liu

{"title":"Clinical features and hematological findings of COVID-19 patients with blood transfusion","authors":"Yanhong Zhang, Shangen Zheng, Qiangqiang Zhou, Yu Zhang, Yao Zheng, Shun Wang, Lei Liu","doi":"10.46701/bg.2020022020125","DOIUrl":"https://doi.org/10.46701/bg.2020022020125","url":null,"abstract":"This paper aims to illustrate the clinical characteristics, hematological findings, and blood transfusion information of Coronavirus disease 2019 (COVID-19) patients. Twenty-three COVID-19 patients were treated and transfused with blood products in Wuhan First Hospital from February 12 to March 20, 2020. The patients were divided into a survivor group and a non-survivor group, respectively, according to whether the patient had been discharged or died. The results demonstrated at the time of initial blood transfusion, that the non-survivor group possessed a lower platelet ( PLT) than that of the survivor group ( P <0.001), and PLT were below the normal range in 6 (85.7%) non-survivor group and in 2 (12.5%) survivor group ( P <0.01). Over half of these patients had abnormalities in fibrinogen (FIB), activated partial thromboplastin time (APTT), prothrombin time (PT), and international normalized ratio (INR), but no significant difference was found between the non-survivor group and survivor group. The non-survivor group had a dramatically higher D-Dimers and disseminated intravascular coagulation (DIC) scores than those of the survivor group ( P <0.01). Six (85.7%) non-survivors but none of the survivors had a DIC score greater than 6 ( P <0.001). Fifteen (93.8%) survivors and 2 (28.6%) non-survivors were transfused with RBC ( P <0.01). The non-survivors (5/7) possessed a higher proportion for using AP than the survivors (2/16). The study suggests that COVID-19 patients who undergo blood transfusion usually possess coagulation dysfunction, and DIC may be closely related to deteriorating clinical outcomes.","PeriodicalId":87226,"journal":{"name":"Blood and genomics","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83108567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The diagnostic value of miRNA-141 in prostate cancer: a systematic review and meta-analysis miRNA-141在前列腺癌中的诊断价值:一项系统综述和荟萃分析

Blood and genomics Pub Date : 2020-01-01 DOI: 10.46701/bg.2020012019131

Yun Ye, Xiao-Hua Yuan, Jian-Jun Wang, Yu-Chen Wang, su-liang li

{"title":"The diagnostic value of miRNA-141 in prostate cancer: a systematic review and meta-analysis","authors":"Yun Ye, Xiao-Hua Yuan, Jian-Jun Wang, Yu-Chen Wang, su-liang li","doi":"10.46701/bg.2020012019131","DOIUrl":"https://doi.org/10.46701/bg.2020012019131","url":null,"abstract":"MiR-141 has gradually demonstrated its value in the diagnosis of prostate cancer. However, the diagnostic parameters applied in previous studies were different. This systematic review was conducted to explore the diagnostic value of miR-141 in prostate cancer. A comprehensive search of related literature in PubMed, Medline, the Cochrane Library and Embase databases was performed. Seven studies were included which assessed the diagnostic value of miR-141 in patients with prostate cancer up to October 31, 2019. Meta - disc version 1.4 and STATA software version 12.0 were used to analyze the data. The pooled sensitivity and specificity were 0.70 (95% CI 0.64~0.75) and 0.73 (95% CI 0.64~0.80), respectively. The positive likelihood ratio (PLR) was 2.88 (95% CI 1.40~5.93), and negative likelihood ratio (NLR) was 0.38 (95% CI 0.20~0.71). The pooled diagnostic odds ratio (DOR) of miR-141 for prostate cancer was observed to be 9.94 (95% CI: 2.55~38.80). The summary area under the receiver operating characteristic (ROC) curve was 0.83 (95% CI: 0.79~0.86). The results of meta - regression suggested that heterogeneity was mainly derived from patient age. The Fagan nomogram results showed a significant increase when correlating miR-141 with the diagnosis of prostate cancer. This meta - analysis suggests that miR-141 has a high diagnostic value for prostate cancer. In future, large - scale prospective studies will be done to verify and evaluate this result.","PeriodicalId":87226,"journal":{"name":"Blood and genomics","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87867871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microchip technology applications for blood group analysis 微芯片技术在血型分析中的应用

Blood and genomics Pub Date : 2020-01-01 DOI: 10.46701/bg.2020022020109

Hongjun Gao, G. Misevic

{"title":"Microchip technology applications for blood group analysis","authors":"Hongjun Gao, G. Misevic","doi":"10.46701/bg.2020022020109","DOIUrl":"https://doi.org/10.46701/bg.2020022020109","url":null,"abstract":"Blood group analysis techniques are some of the most in demand immunological applications in clinical transfusion praxis and organ transplantation. In order to aid the advance towards higher throughput and increased sensitivity, analytical solutions dealing with a minimal amount of blood samples and the miniaturization of diagnostic equipment using microchip technologies have been evolving into an optimal solution. Here we review fabrication technologies for various types of microstructure on microchips, related operating procedures, and characterization approaches. Our focus is on examples of microchip technology and instrumentation used for blood group analysis ranging from classical serological methods of glycoprotein detection and solid phase assays, to nucleic acid amplification techniques. Molecular typing using microchip-based techniques is emerging as a supplement to standard serological methods. Microchip technology will play its key role to support blood group analysis at the molecular scale by using microliters of blood samples for extremely sensitive, quantitative, and high throughput analyses. single nucleotide polymorphisms; NAT, nucleic acid amplification techniques; PCR, polymerase chain reaction; LR-PCR, long-range polymerase chain reaction; Rh, Rhesus Macacus","PeriodicalId":87226,"journal":{"name":"Blood and genomics","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89322626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The RHD variants in Chinese population 中国人群中的RHD变异

Blood and genomics Pub Date : 2020-01-01 DOI: 10.46701/bg.2020012020106

Ming-Ze Gao, Yuping Chen

引用次数: 2

Increased SLC4A11 expression is associated with poor prognosis of gastric cancer SLC4A11表达升高与胃癌预后不良相关

Blood and genomics Pub Date : 2020-01-01 DOI: 10.46701/bg.2020012019119

Mengjing Sun, Y. Qi, Q. Jin, Huijun Zhu, Yao-Xian Wang, Xiaohui Jiang

{"title":"Increased SLC4A11 expression is associated with poor prognosis of gastric cancer","authors":"Mengjing Sun, Y. Qi, Q. Jin, Huijun Zhu, Yao-Xian Wang, Xiaohui Jiang","doi":"10.46701/bg.2020012019119","DOIUrl":"https://doi.org/10.46701/bg.2020012019119","url":null,"abstract":"Solute carrier family 4 member 11 (SLC4A11) is a member of the SLC4 family of bicarbonate transporters, which have been reported to encode sodium-coupled bicarbonate transporters. In this study, SLC4A11 mRNA and protein expression in stomach tissue of 441 patients and 5 cell lines were detected by quantitative reverse transcription-polymerase chain reaction, Western blot, and immunohistochemistry. SLC4A11 expression was predictively analyzed using Oncomine online resources. The results revealed that SLC4A11 mRNA and protein levels were both significantly higher in gastric cancer than those in normal gastric tissues (both P<0.001). Statistical analysis revealed that SLC4A11 high expression was associated with Her-2 expression (P=0.018), N stage (P=0.031), TNM stage (P=0.015), and preoperative CEA levels (P=0.017). In univariate and multivariate analyses, SLC4A11 overexpression and advanced N stage showed a great prognostic value for 5-year survival. Our results suggest that SLC4A11 plays an important role in gastric carcinoma development and progression and that SLC4A11 could be a significant prognostic marker for gastric cancer patients.","PeriodicalId":87226,"journal":{"name":"Blood and genomics","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85449872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of the ABO blood group with certain human diseases ABO血型与某些人类疾病的关系

Blood and genomics Pub Date : 2020-01-01 DOI: 10.46701/bg.2020022020108

Surui Liu, Xiaoya Yang, Gengyin Wang

引用次数: 0

Association between polymorphism of the cyclin E1 gene and susceptibility to hepatocellular carcinoma in Chinese Han population of Hubei 细胞周期蛋白E1基因多态性与湖北汉族肝癌易感性的关系

Blood and genomics Pub Date : 2019-01-01 DOI: 10.46701/apjbg.2019022019118

Qing Liu, Jia-Yan Ling, Qilong Song, Xiaodong Li, Chizhi Zhang, M. Xiao, Jie Huang, Shuxian Han, Ling Liu, Xiao-Mei Peng, Jie-xiong Tan, Yu Wang, Ouyang Li, Youyun Zhao, Jianhua Wu

{"title":"Association between polymorphism of the cyclin E1 gene and susceptibility to hepatocellular carcinoma in Chinese Han population of Hubei","authors":"Qing Liu, Jia-Yan Ling, Qilong Song, Xiaodong Li, Chizhi Zhang, M. Xiao, Jie Huang, Shuxian Han, Ling Liu, Xiao-Mei Peng, Jie-xiong Tan, Yu Wang, Ouyang Li, Youyun Zhao, Jianhua Wu","doi":"10.46701/apjbg.2019022019118","DOIUrl":"https://doi.org/10.46701/apjbg.2019022019118","url":null,"abstract":"This study aimed to explore the relationship between CCNE1 gene single nucleotide polymorphisms (SNP rs1406 and rs3218038) and the incidence of hepatitis B virus-related hepatocellular carcinoma (HCC) in the Chinese Han population in Hubei. A total of 663 subjects, including 173 HCC patients, 172 HBV-related liver cirrhosis (LC) patients, 162 asymptomatic HBV carriers (AsC), and 156 healthy controls, participated in the study. Genotyping of CCNE1 rs1406 and rs3218038 polymorphisms was done by illumina second generation sequence method.Our findings showed that rs1406 G>T variant decreased the risk of HCC (OR 0.710, P=0.035 G vs T), and no significant differences were found between rs3218038 SNP and HCC risk using the χ test. Furthermore, stratified analysis revealed that differences in genotype frequencies were related to gender. Women who carried the CCNE1 GT genotype were significantly associated with a decreased risk of HCC, compared with healthy controls carrying the GG genotype (additive model, OR 0.378,P=0.030).The results suggest that the rs1406 G allele and CCNE1 rs1406 polymorphism produce an increased the risk of HCC in comparison with the T allele. Whereas, the GT genotype is a protective factor in the development of HCC in female patients.","PeriodicalId":87226,"journal":{"name":"Blood and genomics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82399281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization and performance validation for the fully automatic blood type analysis system 全自动血型分析系统的优化与性能验证

Blood and genomics Pub Date : 2019-01-01 DOI: 10.46701/apjbg.2019012019112

Rongrong Pang, Libo Zhang, Yong Wang, Wenping Han, Min Huang, Qiang Fu

引用次数: 0