Postepy higieny i medycyny doswiadczalnej (Online)最新文献_第10页

Molecular diagnostics of periodontitis. 牙周炎的分子诊断。

Postepy higieny i medycyny doswiadczalnej (Online) Pub Date : 2017-01-28 DOI: 10.5604/17322693.1229820

I. Korona-Głowniak, Radosław Siwiec, M. Berger, A. Malm, J. Szymańska

{"title":"Molecular diagnostics of periodontitis.","authors":"I. Korona-Głowniak, Radosław Siwiec, M. Berger, A. Malm, J. Szymańska","doi":"10.5604/17322693.1229820","DOIUrl":"https://doi.org/10.5604/17322693.1229820","url":null,"abstract":"The microorganisms that form dental plaque are the main cause of periodontitis. Their identification and the understanding of the complex relationships and interactions that involve these microorganisms, environmental factors and the host's health status enable improvement in diagnostics and targeted therapy in patients with periodontitis. To this end, molecular diagnostics techniques (both techniques based on the polymerase chain reaction and those involving nucleic acid analysis via hybridization) come increasingly into use. On the basis of a literature review, the following methods are presented: polymerase chain reaction (PCR), real-time polymerase chain reaction (real-time PCR), 16S rRNA-encoding gene sequencing, checkerboard and reverse-capture checkerboard hybridization, microarrays, denaturing gradient gel electrophoresis (DGGE), temperature gradient gel electrophoresis (TGGE), as well as terminal restriction fragment length polymorphism (TRFLP) and next generation sequencing (NGS). The advantages and drawbacks of each method in the examination of periopathogens are indicated. The techniques listed above allow fast detection of even small quantities of pathogen present in diagnostic material and prove particularly useful to detect microorganisms that are difficult or impossible to grow in a laboratory.","PeriodicalId":87132,"journal":{"name":"Postepy higieny i medycyny doswiadczalnej (Online)","volume":"58 1","pages":"47-56"},"PeriodicalIF":0.0,"publicationDate":"2017-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75368946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Visual evoked potentials in diagnostics of optic neuropathy associated with renal failure. 视觉诱发电位在肾衰竭相关视神经病变诊断中的应用。

Postepy higieny i medycyny doswiadczalnej (Online) Pub Date : 2017-01-22 DOI: 10.5604/17322693.1229345

Małgorzata Jurys, Sebastian Sirek, A. Kolonko, D. Pojda-Wilczek

{"title":"Visual evoked potentials in diagnostics of optic neuropathy associated with renal failure.","authors":"Małgorzata Jurys, Sebastian Sirek, A. Kolonko, D. Pojda-Wilczek","doi":"10.5604/17322693.1229345","DOIUrl":"https://doi.org/10.5604/17322693.1229345","url":null,"abstract":"Chronic renal failure is associated with many neurological complications. Due to accumulation of uremic neurotoxins axonal degeneration with its secondary demyelination occurs, which results in development of polineuropathy in 60‑100% of patients with chronic renal failure. One of the most severe peripheral neuropathy is optic neuropathy. It is associated with visual deterioration and reduction in quality of life. Symptoms of the optic neuropathy may appear either before or after dialysis therapy. They often worsen after renal transplant, probably due to immunosuppressive regimen. Early diagnostics of the optic neuropathy became possible by using visual evoked potentials (VEP). This reliable, sensitive and noninvasive technique provides a direct measure of subclinical impairment of visual pathways. Among hemodialysed or immunosupressed patients one can observe abnormal VEP parameters - especially prolonged latency of the P100 component, less often fluctuation of its amplitude. These alterations are pronounced even if clinical examination reveals no abnormalities. This review presents a summary of current use of visual evoked potentials in monitoring of patients with chronic renal failure.","PeriodicalId":87132,"journal":{"name":"Postepy higieny i medycyny doswiadczalnej (Online)","volume":"6 1","pages":"32-39"},"PeriodicalIF":0.0,"publicationDate":"2017-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74310739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Effects of endogenous cardioprotective mechanisms on ischemia-reperfusion injury. 内源性心脏保护机制对缺血再灌注损伤的影响。

Postepy higieny i medycyny doswiadczalnej (Online) Pub Date : 2017-01-10 DOI: 10.5604/17322693.1228267

Marcin Kunecki, W. Płazak, P. Podolec, K. Golba

{"title":"Effects of endogenous cardioprotective mechanisms on ischemia-reperfusion injury.","authors":"Marcin Kunecki, W. Płazak, P. Podolec, K. Golba","doi":"10.5604/17322693.1228267","DOIUrl":"https://doi.org/10.5604/17322693.1228267","url":null,"abstract":"Ischemic heart disease have been remarked as a leading cause of morbidity and mortality in adults. Early restoration of cardiac perfusion is necessary to restore perfusion of ischemic heart muscle. Effective revascularization reduce mortality by limiting myocardial necrosis at the acute phase of the cardiac infarction. However, reperfusion may induce a cascade of pathophysiological reactions causing the increase of the infarct area of the myocardium This phenomenon known as ischemia-reperfusion injury is responsible for up to 50% of the final infarct size. Sequences of brief episodes of nonlethal ischemia and reperfusion applied before (preconditioning - IPC) or after (postconditioning - POC) the coronary occlusion are well documented to reduce the ischemiareperfusion injury. These phenomena improve cardiac function by mobilizing the molecular and cellular mechanisms limiting reperfusion injury. The mechanisms underlying IPC or POC are still not clarified, but strong experimental evidence suggests that opioids may be the part of the endogenous cardioprotective response to I/R injury. Stimulation of opioid receptors activates related to POC mechanisms affecting protection to the ischemic myocardium, while the use of non-selective opioid receptor antagonist - naloxone reduces this effect. There is no consensus that the subtype of opioid receptor is responsible for the protection of the human heart muscle. Morphine may reduce cardiac preload by peripheral vasodilatation. Numerous studies show a direct cardioprotective effect of the opioid pathway in ischemic conditions. Opioids act via membrane receptors: μ, δ, κ. The predominant subtype in the human cardiac cells are μ- and δ - opioid receptors. It has been hypothetized that opioid receptor activation exerts cardioprotection in human heart muscle pathway what may give insight into the explanation of the protective mechanisms in the acute myocardial infarction.","PeriodicalId":87132,"journal":{"name":"Postepy higieny i medycyny doswiadczalnej (Online)","volume":"1 1","pages":"20-31"},"PeriodicalIF":0.0,"publicationDate":"2017-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89467910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

The role of interleukin 15 in neoplasia. 白细胞介素15在肿瘤中的作用。

Postepy higieny i medycyny doswiadczalnej (Online) Pub Date : 2017-01-10 DOI: 10.5604/17322693.1228266

Małgorzata Chłopek, A. Kowalik, S. Góźdź, K. Koziak

{"title":"The role of interleukin 15 in neoplasia.","authors":"Małgorzata Chłopek, A. Kowalik, S. Góźdź, K. Koziak","doi":"10.5604/17322693.1228266","DOIUrl":"https://doi.org/10.5604/17322693.1228266","url":null,"abstract":"Interleukin 15 is a pleiotropic cytokine of the four α helix bundle family. Binding to a heterotrimeric receptor complex, which consists of a unique, high affinity IL‑15Rα‑chain and IL-2/IL-15Rβ and IL‑2Rγ chains, IL‑15 activates signaling pathways leading to activation and proliferation of T and B cells, as well as natural killer cells. At the same time, IL‑15 protects effector cells from T regulatory cells and does not induce immune tolerance. The significant regulatory action of IL‑15 on the immune system provides new opportunities for development of anti‑cancer therapies. As documented in many experiments using different tumor models, IL‑15 enhances antitumor effects. To improve the efficiency of IL‑15, several strategies, including combination with other anti‑cancer therapies such as chemotherapy, additional use of antibodies (anti‑PD‑L1, anti‑CTLA‑4, anti‑CD40), or other cytokines, have been evaluated. Increased anti‑tumor activity can also be obtained by using IL‑15 agonists. However, acting as a growth factor for immune cells but also for tumor cells, IL‑15 may promote their proliferation, survival and dissemination. Of significance seems the role of IL‑15 in the pathogenesis of hematological malignancies, which is due to the involvement in the proliferation and differentiation of NK, T and B cells. Currently, several experimental strategies are available to block biological activity of IL‑15. Among compounds inhibiting the activity of IL‑15 are not only monoclonal antibodies interacting directly with the cytokine or with IL‑15R subunits, but also mutant forms of IL‑15 and protein constructs.","PeriodicalId":87132,"journal":{"name":"Postepy higieny i medycyny doswiadczalnej (Online)","volume":"61 1","pages":"5-19"},"PeriodicalIF":0.0,"publicationDate":"2017-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72820118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Mitochondrial functionality in female reproduction. 线粒体在女性生殖中的功能。

Postepy higieny i medycyny doswiadczalnej (Online) Pub Date : 2017-01-04 DOI: 10.5604/01.3001.0010.3848

Łukasz Gąsior, Regina Daszkiewicz, Mateusz Ogórek, Zbigniew Polański

引用次数: 4

Activity of urine arylsulfatase A in brain‑dead graft donors is a predictor of early and late graft function. 脑死亡移植供者尿芳基硫酸酯酶A的活性是早期和晚期移植功能的预测因子。

Postepy higieny i medycyny doswiadczalnej (Online) Pub Date : 2017-01-04 DOI: 10.5604/17322693.1227823

E. Kwiatkowska, L. Domański, J. Bober, K. Safranow, A. Pawlik, K. Ciechanowski

{"title":"Activity of urine arylsulfatase A in brain‑dead graft donors is a predictor of early and late graft function.","authors":"E. Kwiatkowska, L. Domański, J. Bober, K. Safranow, A. Pawlik, K. Ciechanowski","doi":"10.5604/17322693.1227823","DOIUrl":"https://doi.org/10.5604/17322693.1227823","url":null,"abstract":"OBJECTIVE\u0000Human lysosomal arylsulfatase A (ASA) is a member of the sulfatase family. Arylsulfatase A is required to degrade sulfatides. Sulfatides occur in the myelin sheets of the central and peripheral nervous system. In this study we evaluated the urine activity of lysosomal enzyme arylsulfatase A in brain‑dead donors as a marker and predictor of short - and long‑term renal allograft function.\u0000\u0000\u0000PATIENTS/METHODS\u0000We analyzed data from kidney recipients who received organs from brain‑dead donors. Data from 40 donors and 68 recipients were analyzed.\u0000\u0000\u0000RESULTS\u0000Urine activity of arylsulfatase A in graft donors correlated positively with creatinine clearance in graft recipients after transplantation: significantly after 30 days (Rs=0.38, p=0.004) and after 3 years (Rs=0.38, p=0.03), and with borderline significance after 14 days (Rs=0.25, p=0.08) and after one year (Rs=0.23, p=0.07).\u0000\u0000\u0000CONCLUSIONS\u0000The results of this study suggest that arylsulfatase A has a protective effect on kidney allograft, and the urine activity of this enzyme in kidney donors correlates positively with graft function.","PeriodicalId":87132,"journal":{"name":"Postepy higieny i medycyny doswiadczalnej (Online)","volume":"6 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2017-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85049210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Clinical usefulness of serum free light chains measurement in patients with multiple myeloma: comparative analysis of two different tests. 血清游离轻链检测在多发性骨髓瘤患者中的临床应用:两种不同检测方法的比较分析

Postepy higieny i medycyny doswiadczalnej (Online) Pub Date : 2017-01-04 DOI: 10.5604/17322693.1229346

T. Kubicki, D. Dytfeld, A. Baszczuk, E. Wysocka, M. Komarnicki, K. Lewandowski

{"title":"Clinical usefulness of serum free light chains measurement in patients with multiple myeloma: comparative analysis of two different tests.","authors":"T. Kubicki, D. Dytfeld, A. Baszczuk, E. Wysocka, M. Komarnicki, K. Lewandowski","doi":"10.5604/17322693.1229346","DOIUrl":"https://doi.org/10.5604/17322693.1229346","url":null,"abstract":"INTRODUCTION\u0000There are two commercially available tests for measurement of serum free light chains (sFLC) in multiple myeloma (MM) patients - Freelite and N Latex FLC. The aim of this study was to perform an assessment and direct comparison of the usefulness of the methods in routine clinical practice.\u0000\u0000\u0000METHODS\u000040 refractory/relapsed MM patients underwent routine disease activity assessment studies, along with sFLC analysis using both assays. Correlation and concordance between the tests and sensitivity of studied methods of sFLC assessment were established. Special attention was focused on sFLC results in patients finally evaluated after completing the treatment.\u0000\u0000\u0000RESULTS\u0000A weak correlation for the measurement of both κ [Passing-Bablok slope (PB) = 0.7681] and λ chains [(PB) = 1.542] was found. Using Bland-Altman plots, a bias of 0.0467 (κ) and -0.2133 (λ) between the measurements was documented. The concordance coefficient equaled 0.87 for κ, 0.62 for λ and 0.52 for κ/λ ratio. Ten patients had an abnormal Freelite assay κ/λ ratio and normal N Latex FLC κ/λ ratio. Three of these patients had negative serum protein electrophoresis results and fulfilled diagnostic criteria of stringent complete remission (sCR) according to N Latex FLC (but not according to Freelite). When the κ/λ ratio obtained by both methods was compared to patients' serum/urine protein electrophoresis and immunofixation results, sensitivity of Freelite and N Latex FLC was established to be 62.5% and 41%, respectively.\u0000\u0000\u0000CONCLUSIONS\u0000There was no strong correlation or concordance between the two assays, and the sensitivity in terms of sFLC detection was different. This may cause problems when diagnosis of sCR is considered.","PeriodicalId":87132,"journal":{"name":"Postepy higieny i medycyny doswiadczalnej (Online)","volume":"16 1","pages":"40-46"},"PeriodicalIF":0.0,"publicationDate":"2017-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73654476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Analysis of the relationship between single nucleotide polymorphism of the CD209, IL-10, IL-28 and CCR5 D32 genes with the human predisposition to developing tick-borne encephalitis. CD209、IL-10、IL-28 和 CCR5 D32 基因的单核苷酸多态性与人类易患蜱传脑炎的关系分析。

Postepy higieny i medycyny doswiadczalnej (Online) Pub Date : 2017-01-04 DOI: 10.5604/01.3001.0010.3856

Piotr Czupryna, Miłosz Parczewski, Sambor Grygorczuk, Sławomir Pancewicz, Joanna Zajkowska, Justyna Dunaj, Maciej Kondrusik, Katarzyna Krawczuk, Anna Moniuszko-Malinowska

{"title":"Analysis of the relationship between single nucleotide polymorphism of the CD209, IL-10, IL-28 and CCR5 D32 genes with the human predisposition to developing tick-borne encephalitis.","authors":"Piotr Czupryna, Miłosz Parczewski, Sambor Grygorczuk, Sławomir Pancewicz, Joanna Zajkowska, Justyna Dunaj, Maciej Kondrusik, Katarzyna Krawczuk, Anna Moniuszko-Malinowska","doi":"10.5604/01.3001.0010.3856","DOIUrl":"10.5604/01.3001.0010.3856","url":null,"abstract":"Introduction: It is known that in the pathogenesis of tick-borne encephalitis (TBE) various molecules play a significant role. The most prominent factors include IL-10, IL-28B, CD-209 and CCR5. It is reasonable to search for genetic predispositions to the development of various clinical forms of TBE related to the genetic variation of IL-10, IL-28B, CD-209 and CCR5. In this study we aimed to search for the relationship between single nucleotide polymorphism in the promoter region of the CD209, IL-10, IL-28 and 32 base pair deletion in CCR5 coding region (Δ 32) with the human predisposition to development of various clinical presentations of TBE. We tried to assess the relation between the presence of particular alleles and genotypes with laboratory and clinical parameters. Material/Methods 59 patients with TBE and 57 people, bitten by a tick who never developed TBE (Polish cohort), were included in the study. To assess the distribution of single nucleotide polymorphisms, TaqMan SNP genotyping assays were used for IL10: rs1800872 and rs1800896, for CD 209 rs4804803 and rs2287886, rs12979860 for IL 28B SNPs according to the manufacturer's protocol using real-time PCR technology on the StepOne thermal cycler. Results Comparison between TBE patients and CG showed that in SNP rs2287886 CD 209 AG heterozygotes were more frequent in the TBE group, while homozygotes GG were more frequent in the CG group. Conclusions SNP rs2287886 CD 209 AG heterozygotes predispose humans to develop TBE. Single nucleotide polymorphism in the promoter region of the CD209, IL-10, IL-28 and CCR5 D32 genes does not correlate with the severity of TBE.","PeriodicalId":87132,"journal":{"name":"Postepy higieny i medycyny doswiadczalnej (Online)","volume":"71 1","pages":"788-796"},"PeriodicalIF":0.0,"publicationDate":"2017-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35501562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

The role of trophic factors and inflammatory processes in physical activity-induced neuroprotection in Parkinson's disease. 营养因子和炎症过程在体育活动诱导的帕金森病神经保护中的作用

Postepy higieny i medycyny doswiadczalnej (Online) Pub Date : 2017-01-04 DOI: 10.5604/01.3001.0010.3850

Ewelina Pałasz, Agnieszka Bąk, Anna Gąsiorowska, Grażyna Niewiadomska

引用次数: 12

Antioxidant and antimicrobial properties of bioactive phytochemicals from cranberry. 蔓越莓生物活性植物化学物质的抗氧化和抗菌特性。

Postepy higieny i medycyny doswiadczalnej (Online) Pub Date : 2016-12-31 DOI: 10.5604/17322693.1227896

M. Baranowska, A. Bartoszek

{"title":"Antioxidant and antimicrobial properties of bioactive phytochemicals from cranberry.","authors":"M. Baranowska, A. Bartoszek","doi":"10.5604/17322693.1227896","DOIUrl":"https://doi.org/10.5604/17322693.1227896","url":null,"abstract":"In the rational human diet, the important role of fruits and vegetables, which are a source of bioactive phytochemicals, is emphasized. Among fruits particular attention, due to a number of documented health-promoting properties, is focused on cranberry. This fruit is characterized by the high content of antioxidant phenolic compounds, which may support the natural antioxidant defense system of the body in the prevention of damage caused by oxidative stress induced by reactive oxygen species (ROS). Therefore, cranberry is suggested for the prevention of civilization diseases such as atherosclerosis, hypertension and cancer, whose etiology is associated directly with oxidative stress. The health-promoting potential of cranberry is also associated with its antibacterial activity resulting from the presence of proanthocyanidins (PAC) type A with documented anti-adherence properties. The best-established medical applications of cranberry fruits are prevention and treatment of bacterial infections of the urinary tract (UTI), infections of gastric mucosa, and infections of the oral cavity. Due to the widespread use of cranberry and pharmaceutical preparations containing PACs in treating UTI, it is very important to evaluate the absorption, bioavailability and metabolism of these compounds in the human body.","PeriodicalId":87132,"journal":{"name":"Postepy higieny i medycyny doswiadczalnej (Online)","volume":"34 1","pages":"1460-1468"},"PeriodicalIF":0.0,"publicationDate":"2016-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74646306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23