Basic & Clinical Cancer Research最新文献_第5页

MicroRNA Promoter Methylation in Colorectal Cancer 结直肠癌中的MicroRNA启动子甲基化

Basic & Clinical Cancer Research Pub Date : 2021-03-14 DOI: 10.18502/BCCR.V11I3.5720

Masoud Asefi, Nayebali Rezvani, Mohammad Hasan Soheilifar, M. Saidijam, Ali Mahdavinezhad

引用次数: 0

PCR-HRM for Detecting JAK2V617F Gene Mutation: Is It a Sensitive Assay? PCR-HRM检测JAK2V617F基因突变:是一种敏感的检测方法吗?

Basic & Clinical Cancer Research Pub Date : 2021-03-14 DOI: 10.18502/BCCR.V11I4.5722

M. Rezaei, M. Toutkaboni, Babak Salimi, S. Seifi, F. Sheikholeslami

{"title":"PCR-HRM for Detecting JAK2V617F Gene Mutation: Is It a Sensitive Assay?","authors":"M. Rezaei, M. Toutkaboni, Babak Salimi, S. Seifi, F. Sheikholeslami","doi":"10.18502/BCCR.V11I4.5722","DOIUrl":"https://doi.org/10.18502/BCCR.V11I4.5722","url":null,"abstract":"Background: A substitution of G to T at nucleotide 1849 in exon 14 of the Janus kinase2 (JAK2) gene is well recognized in myeloproliferative neoplastic disorders (MPNs). Based on WHO guidelines, detection of the mutation is very important to confirm the disease in suspected patients. Methods: Eighty-seven patients with different background diseases were tested for JAK2 V617F mutation by four different methods, including polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), amplification refractory mutation system (ARMS), polymerase chain reaction-high resolution melting (PCR-HRM), and two different commercial kits. Results: The mean age of patients was 53.38±17.43 years, 72.4% were males, and 37.6% were females. JAK2 mutation was detected in 16 patients (18.3%). Of those, 7 (43.75%) suffered from PV, 5 (31.25%) from ET, 3 (18.75%) from PMF, and 1 (6.15%) from unclassified neoplastic disorders. The frequency of JAK2 mutation was 71.4% (5/7) in PV, 80% (4/5) in ET, and 66.7% (2/3) in PMF patients. The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and GE of PCR-HRM for detection of the JAK2 mutation was 86.7%, 100%, 100%, 97.3%, and 97.7%, respectively. While the sensitivity, specificity, PPV, NPV, and GE of PCR-RFLP were 93.3%, 80.5%, 50%, 98.3%, and 82.7%, respectively. On the other hand, the sensitivity, specificity, PPV, NPV, and GE of ARMS assays were evaluated by about 80%, 96%, 100%, 96%, and 96.5%, respectively. Conclusion: This study showed that PCR-HRM was a more sensitive assay to detect the JAK2 V617F mutation than the other assays. So, it can be used as a quick, easy, and effective method for screening the JAK2 V617F mutation in patients with MPNs disorders. PCR-RFLP must accompany it as a gold standard method for confirmation of the mutation of JAK2 V617F.","PeriodicalId":8706,"journal":{"name":"Basic & Clinical Cancer Research","volume":"95 1","pages":"173-181"},"PeriodicalIF":0.0,"publicationDate":"2021-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78204419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Benign Multi-Cystic Peritoneal Mesothelioma: A Case Report 良性多囊腹膜间皮瘤1例报告

Basic & Clinical Cancer Research Pub Date : 2019-06-23 DOI: 10.18502/bccr.v11i2.1659

A. Shaghaghi, Roya Yousefian

引用次数: 2

Pectoralis Major Myocutaneous Flap Reconstruction with Deltopectoral Flap Incision in the Surgical Treatment of Oral Cancer 胸三角皮瓣切开重建胸大肌肌皮瓣在口腔癌手术治疗中的应用

Basic & Clinical Cancer Research Pub Date : 2019-06-23 DOI: 10.18502/BCCR.V11I2.1657

M. M. Davudov, I. Harirchi, Z. Mirzajani

{"title":"Pectoralis Major Myocutaneous Flap Reconstruction with Deltopectoral Flap Incision in the Surgical Treatment of Oral Cancer","authors":"M. M. Davudov, I. Harirchi, Z. Mirzajani","doi":"10.18502/BCCR.V11I2.1657","DOIUrl":"https://doi.org/10.18502/BCCR.V11I2.1657","url":null,"abstract":"Background: One of the main goals of patient management is the reconstruction of tissue defects following surgical resection for oral cancer. A variety of flaps used for this purpose, the most frequent being a Pectoralis Major Myocutaneus Flap (PMMF). This flap is currently widely used for the reconstruction of different defects of the head and neck. Methods: Surgical resection of the primary tumor, as well as neck dissection, performed on 29 patients with oral squamous cell carcinoma between 2011–2017 in City Hospital #1 and Surgical Training Clinic of Azerbaijan Medical University. PMMF used for the reconstruction of defects using a deltopectoral flap incision. Results: In 22 patients (75.8 %) primary healing occurred following a PMMF. Four patients (13.8 %) suffered PMMF necrosis and were took back to the operating room for secondary reconstruction with a deltopectoral flap. In three patients (10.3%) the skin of the flap underwent necrosis, resulting in the development of an orocutaneous fistula. Conclusion: Use of PMMF with deltopectoral flap incision enables deltopectoral flap availability. This flap can then used early on or if PMMF fails.","PeriodicalId":8706,"journal":{"name":"Basic & Clinical Cancer Research","volume":"82 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90292256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Metabolomics in Cancer 癌症代谢组学研究

Basic & Clinical Cancer Research Pub Date : 2019-06-09 DOI: 10.18502/BCCR.V11I2.1654

M. Mobasheri

引用次数: 0

Challenges with the Emergency Departments Use among Cancer Patients; a Mini Review 癌症患者使用急诊科面临的挑战迷你评论

Basic & Clinical Cancer Research Pub Date : 2019-05-26 DOI: 10.18502/BCCR.V11I2.1658

M. Mobasheri, Rahim Behtar, S. Sadighi

引用次数: 0

Treatment of Severe Urinary Incontinence following Radical Prostatectomy: Experience with Bioceram as a Bulking Agent 根治性前列腺切除术后严重尿失禁的治疗:生物胶作为膨胀剂的经验

Basic & Clinical Cancer Research Pub Date : 2019-05-09 DOI: 10.18502/bccr.v11i2.1656

M. Ayati, E. Amini, M. Nowroozi, Seyed Ali Momeni, S. O. Moghadam, R. S. Damavand

{"title":"Treatment of Severe Urinary Incontinence following Radical Prostatectomy: Experience with Bioceram as a Bulking Agent","authors":"M. Ayati, E. Amini, M. Nowroozi, Seyed Ali Momeni, S. O. Moghadam, R. S. Damavand","doi":"10.18502/bccr.v11i2.1656","DOIUrl":"https://doi.org/10.18502/bccr.v11i2.1656","url":null,"abstract":"Background: To evaluate the efficacy of Bioceram injection in men with severe stress urinary incontinence following radical prostatectomy. Methods: A total of 18 patients underwent retrograde injection of Bioceram for severe stress urinary incontinence following radical prostatectomy. Evaluation by pad test, international consultation on the Incontinence Questionnaire - Short Form (ICIQ-SF) and American Urology Association Symptom Score - Quality of Life (AUASS-QOL) was performed before and after injection therapy. Patients were considered cured if they were using no pads or only one safety pad per day. Results: Of 18 patients, 14 had received postoperative external beam radiation therapy. Furthermore, 5 patients required transurethral incision due to simultaneous stricture of the urethrovesical anastomosis. The baseline daily pad count changed from a mean of 6.1± 0.8 to 5.3 ± 1.7 (p = 0.010). None of the patients were cured and only 3 patients showed signs of improvement following injection. Conclusion: In patients with severe urinary incontinence, treatment with bulking agent injection is associated with modest efficacy.","PeriodicalId":8706,"journal":{"name":"Basic & Clinical Cancer Research","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85482216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Estrogen Receptor Alpha Gene Single Nucleotide Polymorphisms, +2464 C/T and -4576A/C, and Breast Cancer Risk, a Hospital-Based Case-Control Study 雌激素受体α基因单核苷酸多态性，+ 2464c /T和-4576A/C与乳腺癌风险，一项基于医院的病例对照研究

Basic & Clinical Cancer Research Pub Date : 2019-05-09 DOI: 10.18502/BCCR.V11I2.1655

A. Amirzargar, M. Sadr, Samira Esmaeili Reykande, E. Mohebbi, Mohammad Shirkhoda, M. Mahmoodi

{"title":"Estrogen Receptor Alpha Gene Single Nucleotide Polymorphisms, +2464 C/T and -4576A/C, and Breast Cancer Risk, a Hospital-Based Case-Control Study","authors":"A. Amirzargar, M. Sadr, Samira Esmaeili Reykande, E. Mohebbi, Mohammad Shirkhoda, M. Mahmoodi","doi":"10.18502/BCCR.V11I2.1655","DOIUrl":"https://doi.org/10.18502/BCCR.V11I2.1655","url":null,"abstract":"Background: Estrogen is a risk factor for the development of breast cancer. The effect of estrogen is primarily mediated by estrogen receptor alpha 1 (ESR1). In this study, we investigated the association between breast cancer risk and the frequency of alleles and genotypes for two ESR1 single nucleotide polymorphisms (SNPs) in breast cancer patients and a healthy control group. Methods: A total of 98 female patients with pathologically confirmed breast cancer and 93 age-matched healthy female controls who were selected from the visitors of the general hospital were recruited in the study. Two ESR1 candidate polymorphisms; +2464 C/T (rs3020314) and -4576 A/C (rs1514348) were selected. The frequency of alleles and genotypes was determined using Quantitative Real-Time PCR assay. Linkage disequilibrium (LD) was assessed for each pair of markers. Using logistic regression, genotype frequencies were estimated as odds ratios with 95% confidence intervals. Results: There was no significant difference in the genotype and allele distributions of ESR1 for SNPs +2464 C/T and SNP -4576 A/C between patients and controls. The frequency of the ESR1 +2464 T/T genotype in case and control groups was 31.6% vs 29.0%, (OR TT/TC: 1.13, 95%CI: 0.58, 2.20; P = 0.69). The frequency of the +2464C allele was 33.9% vs 35.2%, (OR C/T: 0.94, 95%CI: 0.60, 1.47; P =0.79). The frequency of the ESR1 -4576C/C genotype in case and control groups was 37.75% vs 33.36%, OR CC/AC: 1.02, 95%CI: 0.51, 1.97; P =0.98). The frequency of the -4576A allele was 36.2% vs 43.6 %, (OR C/A: 0.73, 95%CI: 0.47, 1.13; P =0.14). Conclusion: The results indicated that ESR1 polymorphism does not show any significant association with breast cancer risk among female Iranian adults.","PeriodicalId":8706,"journal":{"name":"Basic & Clinical Cancer Research","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81345023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Direct Medical Care Costs Associated with Gastric Cancer in a Third-level Hospital in Iran 伊朗某三级医院胃癌直接医疗费用分析

Basic & Clinical Cancer Research Pub Date : 2019-01-16 DOI: 10.18502/BCCR.V11I1.1650

S. Mohammadpur, M. Yousefi, H. Ebrahimipur, Touraj Harati-Khalilabad, H. Haghighi, M. Kiani, S. Shahidsales, A. Taghipour

{"title":"The Direct Medical Care Costs Associated with Gastric Cancer in a Third-level Hospital in Iran","authors":"S. Mohammadpur, M. Yousefi, H. Ebrahimipur, Touraj Harati-Khalilabad, H. Haghighi, M. Kiani, S. Shahidsales, A. Taghipour","doi":"10.18502/BCCR.V11I1.1650","DOIUrl":"https://doi.org/10.18502/BCCR.V11I1.1650","url":null,"abstract":"Background: Approximately 20 million individuals are afflicted with cancer worldwide. Gastric cancer is the fourth leading cause of death in the world today. The aim of this study was to evaluate the direct medical care costs of gastric cancer patients in a tertiary teaching hospital in Iran. Methods: The present study is descriptive-analytical, carried out in two main stages. In the first stage we designed a form based on valid international guidelines. The second step identified the costs of diagnosis and treatment of gastric cancer. To analyze the cost data, descriptive statistics such as mean and standard deviation were utilized. We used nonparametric statistical tests such as Mann-Whitney, Wilcoxon in SPSS 16 software for data analysis. Results: In this study, the records of 449 gastric cancer patients who had referred to Omid tertiary teaching hospital of Mashhad between the years 2005 and 2015 were studied. According to the results, the highest average costs were related to patient hospitalization costs. Based on the significance level of the Mann Whitney test, no remarkable difference could be seen in the total costs of metastatic and non-metastatic patients (P-value: P> 0.05). Conclusion: Organizations such as: insurance agencies, charities and financial institutions need to adopt new policies to reduce patients’ expenditures, remove financial barriers and prevent patients from facing catastrophic costs.","PeriodicalId":8706,"journal":{"name":"Basic & Clinical Cancer Research","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79725548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutation Analysis of Exon 23 of the PTCH Tumor Suppressor Gene in Multiple Basal Cell Carcinoma Patients with a History of Radiodermatitis 有放射性皮炎病史的多发性基底细胞癌患者PTCH肿瘤抑制基因23外显子突变分析

Basic & Clinical Cancer Research Pub Date : 2019-01-14 DOI: 10.18502/BCCR.V11I1.1651

Sara Mirhadi, A. Abbasi, M. Mobasheri, H. Moslehi, M. Modarressi

{"title":"Mutation Analysis of Exon 23 of the PTCH Tumor Suppressor Gene in Multiple Basal Cell Carcinoma Patients with a History of Radiodermatitis","authors":"Sara Mirhadi, A. Abbasi, M. Mobasheri, H. Moslehi, M. Modarressi","doi":"10.18502/BCCR.V11I1.1651","DOIUrl":"https://doi.org/10.18502/BCCR.V11I1.1651","url":null,"abstract":"Background: Basal Cell Carcinoma (BCC) with its slow-growing and rarely metastatic nature is the most common human neoplasm. Multiple BCCs mostly result from germline mutations in the tumor suppressor gene, PTCH with a genetic transmission pattern. Multiple BCCs may also originate from radiodermatitis which is a significant side effect of ionizing radiation exposure delivered to the skin in various skin treatments. PTCH is a critical member of the Sonic Hedgehog signalling pathway and mutations in this gene have been reported in as many as 40-80% of skin cancers. Exon number 23 is a critical exon in the function of the PTCH protein. Mutations have been reported in codon 1315 of PTCH in non-melanoma skin cancers. Methods: We assessed mutations in exon 23 of the PTCH gene by polymerase chain reaction and direct sequencing in the peripheral blood cells of 10 patients with multiple BCCs. All of the subjects were selected from among patients with a history of radiation exposure and subsequent radiodermatitis. Results: Direct sequencing revealed a Cytosine to Thymine mutation in codon 1315 of the PTCH gene in 60% of patients, 50% of which were heterozygotes, possessing both the C and T allele, and 10% were homozygotes for the T allele in the same position. Four subjects (40%) were normal homozygotes of the C allele, similar to the normal population. Conclusion: Mutations with ID: rs 3575564 were detected in codon 1315 which transform the proline amino-acid to leucine in the PTCH protein. This transformation may affect the normal function of the PTCH protein, as reported previously. Patients with multiple BCCs who had a history of radiation exposure show a transformation from cytosine to thymine in codon number 1315 of the PTCH gene in their peripheral blood cells. Subsequent assessment of BCC tissues will clarify the somatic mutagenesis effects of radiation.","PeriodicalId":8706,"journal":{"name":"Basic & Clinical Cancer Research","volume":"87 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89028890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0