Arkhiv patologii最新文献_第5页

[Exosomes' role in intercellular interactions in different variants of lung injury in fatal cases of COVID-19]. [外泌体在 COVID-19 死亡病例肺损伤不同变体中的细胞间相互作用中的作用]。

Arkhiv patologii Pub Date : 2024-01-01 DOI: 10.17116/patol20248602122

E A Zarubin, E A Kogan, N V Zharkov, A M Avdalyan, D N Procenko

{"title":"[Exosomes' role in intercellular interactions in different variants of lung injury in fatal cases of COVID-19].","authors":"E A Zarubin, E A Kogan, N V Zharkov, A M Avdalyan, D N Procenko","doi":"10.17116/patol20248602122","DOIUrl":"https://doi.org/10.17116/patol20248602122","url":null,"abstract":"Background: Extracellular vesicles are surrounded by a phospholipid bilayer, carrying various active biomolecules and participating in many physiological and pathological processes, including infectious ones.Objective: To research the role of exosomes in intercellular interactions in the pathogenesis of various types of lung damage in fatal cases of COVID-19.Material and methods: We conducted a clinical and morphological analysis of 118 fatal cases caused by coronavirus infection in Moscow. We selected 32 cases with morphological signs of various types of lung lesions for immunohistochemical reaction (IHC) with antibodies against tetraspanin proteins (CD63, CD81), which are involved in the assembly of exosomes, as well as with antibodies against viral proteins: nucleocapsid and spike protein. We determined the main producing cells of extracellular vesicles and cells containing viral proteins, carried out their comparison and quantitative analysis.Results: IHC reaction with antibodies against CD63 showed cytoplasmic granular uniform and subapical staining of cells, as well as granular extracellular staining. We determined similar staining using antibodies against viral proteins. Extracellular vesicles were found in the same cells as viral proteins. The main producing cells of vesicles and cells containing viral proteins were found to be macrophages, type II pneumocytes, and endothelial cells.Conclusion: Taking into account the results of the literature, the localization of viral proteins and extracellular vesicles in the same cells indicates the key role of vesicles in the pathogenesis of various forms of lung damage by the SARS-CoV-2 virus, in the dissemination of the pathogen in the organism, which leads to interaction with the adaptive immune system and the formation of immunity.","PeriodicalId":8548,"journal":{"name":"Arkhiv patologii","volume":"86 2","pages":"22-29"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140854255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The algorithm for morphological assessment of malignant potential of adrenocortical tumors using mathematical modeling method]. [利用数学建模方法对肾上腺皮质肿瘤恶性潜能进行形态学评估的算法]。

Arkhiv patologii Pub Date : 2024-01-01 DOI: 10.17116/patol20248603121

L S Urusova, N V Pachuashvili, E E Porubayeva, A R Elfimova, D G Beltsevich, A Chevais, T A Demura, N G Mokrysheva

{"title":"[The algorithm for morphological assessment of malignant potential of adrenocortical tumors using mathematical modeling method].","authors":"L S Urusova, N V Pachuashvili, E E Porubayeva, A R Elfimova, D G Beltsevich, A Chevais, T A Demura, N G Mokrysheva","doi":"10.17116/patol20248603121","DOIUrl":"https://doi.org/10.17116/patol20248603121","url":null,"abstract":"Objective: To develop the mathematical model with high sensitivity and specificity to assess the malignant potential of adrenal cortical tumors, which can be used to diagnose adrenocortical carcinoma (ACC) in adults.Material and methods: Pathomorphological examination of surgical and consultative material of adrenocortical neoplasms was carried out. All cases were verified according to the WHO Classification of adrenal gland tumors (5th ed., 2022), the tumor's histogenesis was confirmed by immunohistochemical examination. Statistical analysis of the histological and immunohistochemical factors in terms of their value in relation to the diagnosis of ACC was carried out on Python 3.1 in the Google Colab environment. ROC analysis was used to identify critical values of predictors. The cut-off point was selected according to the Youden`s index. Logistic regression analysis using l1-regularisation was performed. To validate the model, the initial sample was divided into training and test groups in the ratio of 9:1, respectively.Results: The study included 143 patients divided into training (128 patients) and test (15 patients) samples. A prognostic algorithm was developed, which represent a diagnostically significant set of indicators of the currently used Weiss scale. The diagnosis is carried out in 3 stages. This mathematical model showed 100% accuracy (95% CI: 96-100%) on the training and test samples.Conclusion: The developed algorithm could solve the problem of subjectivity and complexity in the interpretation of some of the criteria of current diagnostic algorithms. The new model is unique in that, unlike others, it allows verification of all morphological variants of ACC.","PeriodicalId":8548,"journal":{"name":"Arkhiv patologii","volume":"86 3","pages":"21-29"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141330261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The role of immunohistochemical examination in the differential diagnosis of atypical tumors and carcinomas of parathyroid glands]. [免疫组化检查在甲状旁腺非典型肿瘤和癌的鉴别诊断中的作用]。

Arkhiv patologii Pub Date : 2024-01-01 DOI: 10.17116/patol2024860415

E I Kim, A A Lavreniuk, L S Urusova, A K Eremkina, A R Elfimova, N G Mokrysheva

{"title":"[The role of immunohistochemical examination in the differential diagnosis of atypical tumors and carcinomas of parathyroid glands].","authors":"E I Kim, A A Lavreniuk, L S Urusova, A K Eremkina, A R Elfimova, N G Mokrysheva","doi":"10.17116/patol2024860415","DOIUrl":"https://doi.org/10.17116/patol2024860415","url":null,"abstract":"Differential diagnosis of atypical parathyroid tumors (APT) and parathyroid carcinomas (PC) is important in determining further management and prognosis. Morphologic diagnosis is sometimes difficult, in which case it is supplemented by immunohistochemical (IHC) examination.Objective: Studying the role of IHC analysis in the differential diagnosis of APT and PC.Material and methods: The study included 44 patients with morphologic diagnosis of the APT established after surgical treatment for primary hyperparathyroidism on the basis of Endocrinology Research Centre during 2018-2023. All cases underwent IHC examination with evaluation of CD31/CD34 and parathormone (PTH) expression for identification of vascular invasion, Ki-67, parafibromin.Results: According to the results of IHC analysis in 8/44 patients (18.2%) the diagnosis of APT was revised in favor of the PC: in 7 of them vascular invasion was detected; in 1 patient the additional series of slices in the surrounding fatty tissue revealed foci of tumor growth, confirmed by positive reaction with antibodies to PTH. According to IHC results, the material was divided into 2 groups: APT and PC. There were no differences in clinical and morphological characteristics, Ki-67% level and parafibromin expression between the groups.Conclusion: Assessment of clinical and laboratory-instrumental data at the preoperative stage does not allow differentiating APT from PC. In case of APT diagnosis and detection of suspicious morphological features, it is necessary to perform IHC examination to exclude PC.","PeriodicalId":8548,"journal":{"name":"Arkhiv patologii","volume":"86 4","pages":"5-12"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141787162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Clinical and laboratory parameters and pathomorphological features of the lungs in patients who have had COVID-19 viral pneumonia]. [COVID-19病毒性肺炎患者肺部的临床和实验室参数及病理形态特征]。

Arkhiv patologii Pub Date : 2024-01-01 DOI: 10.17116/patol20248601127

A S Kontorshchikov, L M Mikhaleva, M A Sharafetdinova, O A Vasyukova, I S Kozlov, R A Vandysheva, L V Kakturskiy

引用次数: 0

[Papillary renal neoplasm with reverse polarity]. [极性相反的乳头状肾肿瘤]。

Arkhiv patologii Pub Date : 2024-01-01 DOI: 10.17116/patol20248601144

E A Dubova, V N Vysotskaya

引用次数: 0

[Patterns of CAF expression in tumors of the genitourinary system]. [泌尿生殖系统肿瘤中 CAF 的表达模式]。

Arkhiv patologii Pub Date : 2024-01-01 DOI: 10.17116/patol20248606128

N A Oleynikova, I N Sycheva, I A Mikhailov, I A Zhestkov, M G Varenzov, P G Malkov

{"title":"[Patterns of CAF expression in tumors of the genitourinary system].","authors":"N A Oleynikova, I N Sycheva, I A Mikhailov, I A Zhestkov, M G Varenzov, P G Malkov","doi":"10.17116/patol20248606128","DOIUrl":"https://doi.org/10.17116/patol20248606128","url":null,"abstract":"Cancer-associated fibroblasts (CAF), as the most important component of the tumor microenvironment, have been the subject of targeted study in recent years. There is increasing evidence of CAF heterogeneity and their different roles in tumor progression. However, there is no single marker identifying CAF, and there is also no understanding of the similarity of CAF markers in various tumors of the genitourinary system.Objective: To evaluate the response of CAF markers - FAP and PDGFRα+β in bladder cancer and prostate cancer.Material and methods: An immunohistochemical study with antibodies to FAP and PDGFRα+β was conducted on material from 34 patients with prostate cancer and 44 patients with bladder cancer in order to identify statistical relationships with stage, morphological characteristics and survival, as well as to understand the applicability of the same markers for cancer of different localizations.Results: An increased FAP reaction was shown in prostate cancer with metastases (p=0.0239), a significantly higher number of recurrences in the group with a high PDGFRa+β (3+) reaction (p=0.0018); the presence of FAP reaction only in invasive bladder cancers (p=0.0094) and bladder cancers with high grade dysplasia (p=0.0000234), the absence of a relationship between the level of PDGFRa+β and clinical and morphological characteristics in bladder cancer.Conclusion: Our results confirm the heterogeneity of CAF in both bladder and prostate tumors and indicate that FAP is one of CAF subpopulation marker.Considering that FAP is expressed in a population of CAFs that lead to resistance to treatment with immune checkpoint inhibitors in melanoma, the FAP marker may also be diagnostically significant for tumors of the genitourinary system, especially given the fact that bladder tumors are treated with the BCG vaccine.","PeriodicalId":8548,"journal":{"name":"Arkhiv patologii","volume":"86 6","pages":"28-35"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Nosological aspects of the petrification and ossification processes in the lungs]. [肺部石化和骨化过程的组织学方面]。

Arkhiv patologii Pub Date : 2024-01-01 DOI: 10.17116/patol20248606163

I V Dvorakovskaya, I S Platonova, T A Novickaya, E M Nepomnyashchaya, S N Bugrov, B M Ariel

引用次数: 0

[Breast granular cell tumor]. [乳腺颗粒细胞瘤]。

Arkhiv patologii Pub Date : 2024-01-01 DOI: 10.17116/patol20248604148

S A Povzun, N Yu Novitskaya

引用次数: 0

[Pyloric gland adenoma - a rare tumor of the upper gastrointestinal tract with a high risk of malignancy]. [幽门腺腺瘤--一种罕见的上消化道肿瘤，恶变风险高]。

Arkhiv patologii Pub Date : 2024-01-01 DOI: 10.17116/patol20248602130

A S Tertychnyy, N V Pachuashvili, D D Protsenko, S T Avraamova, D P Nagornaya, P V Pavlov, A P Kiryukhin, A A Fedorenko

{"title":"[Pyloric gland adenoma - a rare tumor of the upper gastrointestinal tract with a high risk of malignancy].","authors":"A S Tertychnyy, N V Pachuashvili, D D Protsenko, S T Avraamova, D P Nagornaya, P V Pavlov, A P Kiryukhin, A A Fedorenko","doi":"10.17116/patol20248602130","DOIUrl":"https://doi.org/10.17116/patol20248602130","url":null,"abstract":"Background: Pyloric gland adenomas (PGA) are rare neoplasms of the gastrointestinal tract. According to the literature, these lesions may be underdiagnosed, and their true frequency of occurrence is underestimated.Objective: Clinical and morphological analysis of eight PGA cases of the upper gastrointestinal tract.Material and methods: The study included 8 cases of detection of PGA. In 7 out of 8 cases, the tumor was diagnosed by examining endoscopic biopsies, in 1 case, PGA was an accidental finding in the surgical material after proximal gastric resection.Results: 6 out of 8 patients were female, the median age was 65 years (minimum 36 years and maximum 78 years). In 6 cases, PDA was localized in the stomach, in 1 - in the esophagus and in 1 - in the duodenum The size of the tumors ranged from 0.6 cm to 7.5 cm. 4 out of 6 stomach tumors appeared on the background of confirmed autoimmune gastritis, 1 - on the background of lymphocytic gastritis. 4 tumors were found in the body of the stomach, 1 - in the cardia, 1 - in the bottom of the stomach. In 2 out of 8 cases, there were signs of malignancy of the tumor with the transition to a highly differentiated adenocarcinoma. According to the results of the IHC study, the absence of a p53 mutation was noted in these cases.Conclusion: PGA should be considered as neoplasms with a high risk of transformation into invasive adenocarcinoma. Increasing the recognition of PGA among pathologists and further understanding of the molecular mechanisms involved in their neoplastic transformation will improve the diagnosis and treatment of this pathology.","PeriodicalId":8548,"journal":{"name":"Arkhiv patologii","volume":"86 2","pages":"30-36"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140847855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Pathology of the kidneys in malignant tumors of various localizations and antitumor therapy]. [不同部位恶性肿瘤的肾脏病理学和抗肿瘤治疗]。

Arkhiv patologii Pub Date : 2024-01-01 DOI: 10.17116/patol20248603159

T A Garkusha, E S Stolyarevich, V A Khorzhevskii, S V Ivliev, M A Firsov

引用次数: 0