Arkhiv patologii最新文献

{"title":"[The effect of acetylamide synthetic peptide HLDF-6 on cell differentiation in breast cancer].","authors":"A A Studenikina, E D Mangazeeva, A P Bogachuk, V M Lipkin, A I Autenshlyus","doi":"10.17116/patol2025870215","DOIUrl":"https://doi.org/10.17116/patol2025870215","url":null,"abstract":"<p><p>The development of drugs with the ability to increase differentiation and reduce tumor malignancy is one of the promising directions in the treatment of breast cancer (BC). As such, Human Leukemia Differentiation Factor (HLDF), a protein consisting of 54 amino acids and contributing to an increase in the degree of differentiation of invasive ductal breast carcinoma cells, can be used. The key disadvantage of the full-size HLDF is its rapid biodegradation. In this connection, the acetylamide form of the peptide (HLDF-6) was synthesized to protect against hydrolysis.</p><p><strong>Objective: </strong>To evaluate the effect of HLDF-6 on cell differentiation in various molecular biological subtypes of breast cancer.</p><p><strong>Material and methods: </strong>The relative content of tumor cells of various degrees of differentiation in biopsies of 33 women with breast cancer when exposed to HLDF-6 tumor was evaluated.</p><p><strong>Results: </strong>HLDF-6 significantly increases the relative content of differentiated cells in the luminal B HER2-negative subtype of breast cancer, without significantly affecting the cells of patients with luminal A and basal-like subtypes.</p><p><strong>Conclusion: </strong>The study revealed the prospects of using HLDF-6 as part of differentiating therapy in patients with luminal B HER2-negative subtype of breast cancer.</p>","PeriodicalId":8548,"journal":{"name":"Arkhiv patologii","volume":"87 2","pages":"5-10"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Morphological markers of mitochondrial dysfunction of the basal plate cytotrophoblast of the placenta in preeclampsia of varying severity].","authors":"Z S Khizrieva, L V Kulida, I A Panova","doi":"10.17116/patol20258702124","DOIUrl":"https://doi.org/10.17116/patol20258702124","url":null,"abstract":"<p><p>Pathomorphological changes in preeclampsia develop in all elements of the functional system \"mother-placenta-fetus\" and play a leading role in the course and outcome of pregnancy.</p><p><strong>Objective: </strong>To identify morphological markers of mitochondrial dysfunction of the basal plate cytotrophoblast of the placenta in women with preeclampsia of varying severity.</p><p><strong>Material and methods: </strong>Comprehensive morphological examination of placentas from women with uncomplicated pregnancy (30), moderate (30) and severe (30) preeclampsia included overview histology, morphometry, immunohistochemistry and transmission electron microscopy.</p><p><strong>Results: </strong>Regardless of the severity of preeclampsia, a leading place in the pathomorphological change in the placenta is occupied by a violation of uteroplacental circulation due to a violation of cytotrophoblast invasion. Immunohistochemical parameters of mitochondrial dysfunction of cytotrophoblast cells of the basal lamina have been determined. A significant decrease in the expression of mtDNA transcription and replication proteins (TFAM, POLG) and a violation of the structure of the respiratory chain (NADH+, cytochrome C) were revealed. These changes were combined with ultrastructural rearrangement of mitochondria, which are the basis of ATP synthesis. In moderate preeclampsia, a decrease in the number of mitochondria was revealed, the ratio of oval and rounded forms of mitochondria associated with swelling of the central parts of the crist changes. In severe preeclampsia, the structural basis of the connection of energy metabolism between mitochondria and the granular endoplasmic reticulum is interrupted. The progressive destruction of mitochondrial crysts with the formation of pseudovesicles ends with total necrosis of ultrastructures.</p><p><strong>Conclusion: </strong>In pregnant women with preeclampsia, mitochondrial dysfunction of the cytotrophoblast cells of the basal lamina was diagnosed, characterized by a decrease in the expression of mtDNA transcription and replication proteins in combination with a violation of the I-III enzymatic complexes of the respiratory chain. The identified immunohistochemical markers are pathogenetically associated with ultrastructural rearrangement of mitochondria in preeclampsia of varying severity.</p>","PeriodicalId":8548,"journal":{"name":"Arkhiv patologii","volume":"87 2","pages":"24-29"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Neuroendocrine tumor of the extrahepatic bile ducts. Case report and literature review].","authors":"D E Suraev, S O Zagagov, N A Savelov, A A Ajazova, D Yu Kanner, O V Paklina","doi":"10.17116/patol20258701141","DOIUrl":"10.17116/patol20258701141","url":null,"abstract":"<p><p>Neuroendocrine neoplasms (NENs) are heterogeneous tumors with a common phenotype. There are two fundamentally different groups of NENs: well differentiated with a low proliferative index, called neuroendocrine tumors (NETs) and poorly differentiated with a high proliferative index, called neuroendocrine carcinoma (NEC). Extrahepatic bile duct NETs (EBNETs) account for 0.2% to 2% of all gastrointestinal NETs and up to 2% of all malignant neoplasms of the extrahepatic bile ducts. To date, 114 cases of EBNETs have been described, with disease-free survival ranging from 2 to 240 months. In most cases, the tumors expressed Chromogranin A, Synaptophysin, CD56, and NSE. This report presents a rare case of an EBNET in a 61-year-old patient following resection of the extrahepatic bile ducts. The description includes data from radiological and ultrasound diagnostic methods, macro- and microscopic characteristics of the tumor, and immunohistochemical profile.</p>","PeriodicalId":8548,"journal":{"name":"Arkhiv patologii","volume":"87 1","pages":"41-47"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143405494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Spectrum of morphological changes in chronic spontaneous urticaria and urticarial vasculitis].","authors":"V A Smolyannikova, O Yu Olisova, K S Teplyakova, A V Filatov, N A Larin","doi":"10.17116/patol20258702130","DOIUrl":"https://doi.org/10.17116/patol20258702130","url":null,"abstract":"<p><p>The prevalence of chronic spontaneous urticaria (CSU) in the population is 0.5%-1%. According to modern guidelines for the management of patients with urticaria, the diagnosis of CSU does not require histological examination. However, in controversial clinical setting requiring differential diagnosis with urticarial vasculitis (UV), pathologists discover a wide range of pathomorphological changes in skin preparations in the absence of generally accepted differential diagnostic criteria. In this connection, it is of interest to study the spectrum of morphological changes in chronic spontaneous urticaria and urticarial vasculitis.</p><p><strong>Objective: </strong>To analyze morphological changes in CSU and UV to improve differential diagnosis in clinical practice.</p><p><strong>Material and methods: </strong>The material of 15 patients with urticarial rashes was analyzed. Comparative analysis of the number of neutrophils and mast cells in skin samples from patients with chronic spontaneous urticaria and urticarial vasculitis was performed.</p><p><strong>Results: </strong>The material of 15 patients with urticarial rashes was analyzed. The group of patients with CSU (<i>n</i>=5) was characterized by the absence of signs of leukocytoclastic vasculitis; endothelial cells and mild of moderate swelling were visualized in all samples, the perivascular infiltrate was located in the upper layer of the dermis and was sparse. And when stained with toluidine blue, a large number of mast cells were noted. In the group of patients with UV (<i>n</i>=10), signs of leukocytoclastic vasculitis of varying severity, dense and deep perivascular neutrophilic infiltrates with a small number of mast cells were visualized.</p><p><strong>Conclusion: </strong>Considering the wide range of morphological changes in the skin in CSU and UV, for the differential diagnosis of diseases, a comprehensive histological examination of preparations in combination with an assessment of the number of mast cells in the dermis and an assessment of the composition of the cellular infiltrate for the number of neutrophils can be recommended.</p>","PeriodicalId":8548,"journal":{"name":"Arkhiv patologii","volume":"87 2","pages":"30-36"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Frequency of MSI, PD-L1 (CPS), HER2 in poorly cohesive gastric carcinomas].","authors":"S N Nered, R O Torosyan, N A Kozlov, H Sun, I G Avdyukhin, P V Kononets, I S Stilidi","doi":"10.17116/patol20258702111","DOIUrl":"https://doi.org/10.17116/patol20258702111","url":null,"abstract":"<p><p>Gastric cancer (GC) is a heterogeneous tumor with various molecular changes. An active search for molecular markers is crucial to determine the effectiveness of drug treatment and prognosis of the disease. Several biomarkers have the greatest clinical significance: HER2, MSI / dMMR, PD-L1 (CPS), EBV and Claudin 18.</p><p><strong>Objective: </strong>To study the frequency of HER2, MSI / dMMR and PD-L1 (CPS) in patients with operable GC depending on the tumor type according to P. Lauren.</p><p><strong>Material and methods: </strong>The study included 600 patients with GC who underwent radical surgical treatment at the N.N. Blokhin National Medical Research Center of Oncology from 2018 to 2023.</p><p><strong>Results: </strong>In the study, the proportion of diffuse GC was 21.5% (120/600). HER2 overexpression was found in 5.2% (5/96) of cases with diffuse GC, 20.4% (37/181) of cases with intestinal GC, 10.1% (7/69) of cases with mixed GC (<i>p</i>=0.0029). The incidence of MSI was 3.3% (4/120) of cases with diffuse GC, 11.2% (28/251) of cases with intestinal GC, 7.3% (7/97) of cases with mixed GC (<i>p</i>=0.0378). PD-L1 expression (CPS> 1) was found in 42.3% (11/26) of cases with diffuse GC, 59.4% (35/59) of cases with intestinal GC, 27.3% (9/33) of cases with mixed GC (<i>p</i>=0.006). In poorly cohesive/signet ring cell cancer MSI occurred in 2.5% (2/79) of cases; HER2 overexpression - in 2.9% (2/68) of cases; PD-L1 (CPS> 1) - in 42.1% (8/19) of cases.</p><p><strong>Conclusion: </strong>Our study demonstrated that in diffuse and poorly cohesive/signet ring cell GC the frequency of occurrence of the above clinically significant tumor biomarkers is lower compared to intestinal/mixed GC.</p>","PeriodicalId":8548,"journal":{"name":"Arkhiv patologii","volume":"87 2","pages":"11-17"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Pathogenesis of fibrosis development in ovarian endometriosis].","authors":"O I Patsap, O V Bratchikova, A V Babkina, M B Khabarova, S A Mikhalev, L M Mikhaleva","doi":"10.17116/patol20258702173","DOIUrl":"https://doi.org/10.17116/patol20258702173","url":null,"abstract":"<p><p>Endometriosis-associated fibrosis is a complex phenomenon, and the underlying mechanisms are still not fully understood. Fibrosis is invariably present in all forms of the disease and contributes to the classic endometriosis-associated symptoms such as chronic pelvic pain and infertility. The purpose of this literature review was to study the role of various cell populations, biological mechanisms and signaling pathways in inducing fibrogenesis of endometriosis lesions. PubMed and MEDLINE searched for studies published in English over the past 5 years that studied fibrosis in ovarian endometriosis.</p>","PeriodicalId":8548,"journal":{"name":"Arkhiv patologii","volume":"87 2","pages":"73-78"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143961828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Microsatellite instability as a possible diagnostic marker of the gastric mucosa dysplasia].","authors":"A V Kononov, V A Rubtsov, M N Parygina, E V Demidova, S I Mozgovoi, E G Pomorgailo, A G Shimanskaya, M V Markelova","doi":"10.17116/patol2025870315","DOIUrl":"https://doi.org/10.17116/patol2025870315","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the MMR system proteins and the MSI status of regenerative (indefinite for dysplasia) and pronounced (epithelial dysplasia) gastric mucosa precancerous lesions in the comparison with cancer to determine their possible potential as a diagnostic markers of gastric mucosa dysplasia.</p><p><strong>Material and methods: </strong>The study included 2 groups of gastric mucosa specimens: (1) 150 biopsy gastric mucosa specimens: 43 with low-grade dysplasia, 32 with high-grade dysplasia, 75 - indefinite for dysplasia; (2) 155 cancer tissue specimens from resected stomachs. Gastric mucosa specimens were examined histologically, immunohistochemically using mouse monoclonal antibodies to the MMR system proteins: MLH-1, MSH2, MSH6, PMS2 (Diagnostic BioSystems, USA). MSI was studied with multiplex PCR evaluation of DNA microsatellites (NR-21, NR-24, NR-27, BAT-25, BAT-26) from paraffin sections, their analysis with capillary electrophoresis. The obtained data were processed with the Statistica 10.0 (StatSoft, USA), presented using descriptive, analytical statistics.</p><p><strong>Results: </strong>MSI was detected by immunohistochemistry in 8% (<i>n</i>=6) of the studied cases of low/high grade dysplasia, which does not have statistically significant differences from the distribution of MSI in the gastric cancer group (12% of microsatellite-unstable cases (<i>n</i>=18)) (<i>p</i>=0.49). MSI was not detected in any indefinite for dysplasia case.</p><p><strong>Conclusion: </strong>Detection of microsatellite instability in gastric mucosa dysplasia indicates the likelihood of its occurrence at the early stages of the carcinogenesis cascade and makes it possible to use it to assess the risk of gastric cancer associated with microsatellite instability. The absence of instability in cases indefinite for dysplasia determines the possibility of its use for differential diagnosis of truly neoplastic and regenerative/reactive changes in the gastric mucosa.</p>","PeriodicalId":8548,"journal":{"name":"Arkhiv patologii","volume":"87 3","pages":"5-16"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Pathomorphological quality assessment of complete mesocolonectomy in right colon cancer].","authors":"I P Reznik, V A Avdeenko, A A Nevolskikh, R F Zibirov, V N Grinevich, S A Ivanov, A D Kaprin","doi":"10.17116/patol20258703162","DOIUrl":"https://doi.org/10.17116/patol20258703162","url":null,"abstract":"<p><p>Complete mesocolonectomy (CME) is the current standard of treatment for colon cancer patients. At the same time, there are currently no clear standards for the pathomorphological assessment of CME quality, allowing for a comprehensive and independent assessment of the quality of surgical treatment.</p><p><strong>Objective: </strong>Creation of a standardized system of pathomorphological assessment of the quality of TMCE based on the developed set of universal criteria.</p><p><strong>Material and methods: </strong>The prospective study included the results of treatment of patients with adenocarcinomas of the right half of the colon, who underwent surgical interventions in the volume of right-sided hemicolectomy (RSHE) in the period from 2022 to 2024. The method of pathomorphological examination included mandatory photo documentation, as well as mesocolonectomy quality assessment using the classification of N. West et al., visual assessment of CME quality using the classification of S. Benz et al., standard microscopic examination to determine the presence of metastatic lymph nodes (LN) lesions, dividing them into groups according to the Japanese classification.</p><p><strong>Results: </strong>The study included 142 patients, 116 (81.7%) of whom underwent laparoscopic interventions, while 105 (73.9%) had D3 lymphodissection. According to the pathomorphological study, the most common (65 cases - 45.8%) tumors were located in the ascending section of the transverse colon, multicentric tumor growth within the colon was detected in 3 (2.1%) cases. The overwhelming majority of patients had stage III (92 patients - 64.9%) and IV (25 patients - 17.6%) clinical stages of the disease.</p><p><p>The median of the studied LN was 48 (12-225), affected - 3 (1-51) LN. LN lesion was detected in 79 patients (55.6%). Damage to the apical LN was found in 9 (6.3%) cases. Unfavorable prognosis factors (perineural growth, lympho- and angiovascular invasion, the presence of tumor deposits and metastases in the lung) were identified in 92 (64.7%) patients.</p><p><p>Good quality of mesocolic fascia isolation (Grade 3) according to West was found in 101 cases (71.1%), true CME (Type 0 according to Benz) was performed in 74 cases (52.1%).</p><p><strong>Conclusion: </strong>The quality assessment of the removed specimen after RSHE should be based on a detailed examination of all removed lymph nodes with division into groups in accordance with the Japanese Clinical Classification, assessment of the plane of intestinal resection according to N. West and the quality of CME according to S. Benz.</p>","PeriodicalId":8548,"journal":{"name":"Arkhiv patologii","volume":"87 3","pages":"62-70"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Gastric tumors with GLI1 gene alterations (plexiform fibromyxoma and gastroblastoma). Case report and literature review].","authors":"I V Sidorov, A S Sharlai, D M Konovalov","doi":"10.17116/patol20258701137","DOIUrl":"10.17116/patol20258701137","url":null,"abstract":"<p><p>Plexiform fibromyxoma (PFM) and gastroblastoma (GB) are rare gastric tumors with a specific <i>MALAT1::GLI1</i> rearrangement, included in the conditional spectrum of neoplasms with alterations of the <i>GLI1</i> gene. The article presents a clinical case of PFM in a 6-year-old girl and a literature review highlighting current data on the morphology, immunophenotype and molecular genetic characteristics of PFM and GB. Despite the common genetic anomaly, differences in the morphology and clinical course of these tumors indicate the need for further research to clarify their relationship and potential reclassification in the light of new data on tumors with <i>GLI1</i> gene abnormalities. Integrating the accumulated knowledge about tumors with <i>GLI1</i> gene alterations into diagnostic algorithms and therapeutic approaches will help improve the treatment outcomes of patients with these rare neoplasms.</p>","PeriodicalId":8548,"journal":{"name":"Arkhiv patologii","volume":"87 1","pages":"37-40"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143405541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Immunohistochemical method of megakaryocytic lineage staining in bone marrow biopsy specimens as an additional pathomorphological differential diagnostic sign of primary myelofibrosis and essential thrombocythemia].","authors":"Z P Asaulenko, Yu A Krivolapov","doi":"10.17116/patol20258701122","DOIUrl":"10.17116/patol20258701122","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate and compare morphometric and histotopographic characteristics of megakaryocytic lineage in preparations stained with H&E or antibodies to CD42b in diagnostic trepanobioptates of bone marrow of patients with primary myelofibrosis and essential thrombocythemia with <i>JAK2</i> or <i>CALR</i> mutation. Analyze the dimensions and quantity of CD42b-positive megakaryocytes in 1 mm² area of section and assess suitability of these parameters as an additional differential pathomorphological criterion.</p><p><strong>Material and methods: </strong>108 trephine biopsies of the bone marrow from patients with primary myelofibrosis (N=53) and essential thrombocythemia (N=55) with <i>JAK2</i> or <i>CALR</i> mutation were selected. Digitized bone marrow slides stained with H&E or antibodies to CD42b (clone EP409) were the object of study. In every sample the average values of perimeter and area of megakaryocytes were analyzed, as well as the average number of megakaryocytes in 1 mm² area of myeloid tissue section. Logistic regression analysis was used to describe the relationship between CD42b-positive megakaryocyte characteristics and disease (primary myelofibrosis or essential thrombocythemia).</p><p><strong>Results: </strong>Immunohistochemical examination of bone marrow biopsy specimens using antibodies to CD42b in comparison with H&E staining allows to multiply the number of identifiable megakaryocytes in myeloid tissue by 3.5-4 times (<i>p</i><0.0001). Statistically significant differences in the mean values of the number of megakaryocytes in 1 mm² of the section area and megakaryocyte perimeter in patients with primary myelofibrosis and essential thrombocythemia have been demonstrated. ROC analysis (AUC=0.84, 95% CI 0.7782-0.9199) justifies the inclusion of the average perimeter size of CD42b-positive megakaryocytes and their number in 1 mm² of the section area in the differential diagnostic panel as an additional pathomorphological criterion.</p><p><strong>Conclusion: </strong>The revealed statistically significant differences in quantitative and geometric characteristics of megakaryocytes allowed to calculate differential threshold values of characteristics of megakaryocytic lineage of myeloid tissue in diagnostic trepanobioptates of bone marrow from patients with primary myelofibrosis and essential thrombocythemia. Counting the number of CD42b-positive megakaryocytes in one field of view at a magnification of 400 times was proposed as an additional pathomorphological differential-diagnostic sign.</p>","PeriodicalId":8548,"journal":{"name":"Arkhiv patologii","volume":"87 1","pages":"22-27"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143405484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}