{"title":"Immune enhancing activity of garlic polysaccharide, selenizing Codonopsis pilosula compounds in chickens","authors":"","doi":"10.35495/ajab.2023.149","DOIUrl":"https://doi.org/10.35495/ajab.2023.149","url":null,"abstract":"Codonopsis pilosula polysaccharide is a main ingredient of Codonopsis. The key component in Condoposis is identified as Codonopsis pilosula . This Codonopsis has multiple functions including stimulation of spleen, lungs and tonifying the middle Jiao and Qi. Allium sativum has functions of regulating vital energy, appetizing, detoxification, and disinfection. Polysaccharide of the bulbous is key component to perform the functions of this bulbous. In current study, the immune enhancing activity of GP, sCPP and GP-sCPP through in-vivo and in-vitro trials was evaluated. For in-vivo trials, a total of 150 one day old white roman chicks were randomly divided into five groups, namely immune control (VC), blank control (BC), GP-sCPP, sCPP and GP groups. Chicks in group GP-sCPP, sCPP and GP was intramuscularly treated with 0.5 mg, 0.5 mg and 4.5 mg of polysaccharide, respectively, in all experimental days, and chicks in VC and BC were treated with equal volume of normal saline injections daily. All chicks were bred according to the standards for the rearing of laying hens. For in-vitro trials, lymphocyte cells were cultured in a 12-well plate along with different concentrations of GP-sCPP, sCPP and GP were supplemented in the cells and incubated at 37.5℃. After that, MTT was employed to measure the cytotoxicity of those polysaccharides. The results showed that GP, sCPP and GP-sCPP could increase immune-enhancing activities by releasing cytokines of IL-2 and IFN-γ. In-vivo experiments in chicks showed significantly higher serum antibody titer, T lymphocyte proliferation, and IFN-γ, IL-2 and IL-6 levels in GP-sCPP group. These results demonstrated that GP-sCPP can significantly promote immune enhancing activity, which could be recognized as a novel candidate for new type of immune modulator.","PeriodicalId":8506,"journal":{"name":"Asian Journal of Agriculture and Biology","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135704685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"β -glucan and antioxidant activities of four edible mushroom extracts from\u0000Thailand","authors":"","doi":"10.35495/ajab.2021.07.285","DOIUrl":"https://doi.org/10.35495/ajab.2021.07.285","url":null,"abstract":"The commercial mushroom extracts from Thailand , Lentinus squarrosulus , Pleurotus sajor-caju , Pleurotus ostreatus, and Volvariella volvacea , could be sources of β-glucan and antioxidant. The objective of this research was to evaluate the β-glucan content and antioxidant activities of the mushroom extracts prepared from different extraction conditions. Various solvents, including water, ethanol, acid, and alkali were used for the mushroom extraction. The mushroom extracts were evaluated for their β-glucan content, total phenolic compounds, and antioxidant properties. Among all extraction conditions, the alkaline extracted (BE) P . ostreatus and P. sajor-caju showed high level of β-glucan content with 25.82 ± 3.87% w/w and 23.08 ± 0.56% w/w, respectively. Large amounts of total phenolic compounds were obtained from V. volvacea and L . squarrosulus extracted with water at 60 o C (W60) as 38.07 ± 2.53 and 34.78 ± 5.69 mg gallic acid equivalent/g extract, respectively. Excellent scavenging of ABTS radicals and FRAP assay were also observed in W60 of V. volvacea as 67.12 ± 4.41 and 36.46 ± 3.44 mg trolox equivalent/g extract, respectively. The metal chelating effect of P. ostreatus was the highest at 66.13 ± 1.63 mg EDTA equivalent/g extract but was not significantly different from V. volvacea (63.76 ± 0.51 mg EDTA equivalent/g extract) ( p > 0.05). In conclusion, alkaline extraction was the most suitable for β -glucan and metal chelating activity extracts whereas water extraction was suitable for antioxidant extracts. These simple extraction conditions could be applied in producing high bioactive compounds and antioxidant active ingredients from local mushrooms for further addition in food and health products.","PeriodicalId":8506,"journal":{"name":"Asian Journal of Agriculture and Biology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49430284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}