{"title":"Light-emitting diode enhances the biomass yield and antioxidant activity of Philippine wild mushroom Lentinus swartzii","authors":"Reyna C. Tiniola","doi":"10.35495/AJAB.2020.08.435","DOIUrl":"https://doi.org/10.35495/AJAB.2020.08.435","url":null,"abstract":"Lentinus swartzii is a basidiomycetous fungus that usually grows on fallen logs during rainy season. In this study, the effects of color light-emitting diode (LED) on the mycelial biomass, fruiting body production, and antioxidant properties of L. swartzii were evaluated. The inoculated culture bottles and fruiting bags were incubated under the three-color LEDs (red, green, and blue), and dark condition (control) in a chamber. Phenolic content and scavenging activity mycelia and fruiting bodies ethanol extracts were also analyzed. In liquid culture, red LED cultures produced the highest mycelial dry weight (0.464 g), while green LED cultures registered the highest number of primordia (6.0). The initiation of primordia was not stimulated under red LED and dark condition. However, in fruiting body production, fruiting bags exposed under red LED showed the shortest period of incubation for mycelial ramification (21.20 days), produced the maximum yield of fruiting body (35.73 g) and biological efficiency (7.14%). All LED mycelial extracts exhibited higher radical scavenging activities (RSA) than catechin, and green LED mycelial extract registered the highest phenolic content (PC) (34.21 mg GAE g sample). The red LED fruiting body extract recorded the highest RSA (28.06%) and PC (26.08 mg GAE g sample). Therefore, cultivation of L. swartzii in red LED chamber is a practical technique for enhancing biomass production and antioxidant properties.","PeriodicalId":8506,"journal":{"name":"Asian Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2021-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42694069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Identification of conserved and novel mature miRNAs in selected crops as future targets for metabolic engineering","authors":"Sidra Aslam","doi":"10.35495/AJAB.2020.12.551","DOIUrl":"https://doi.org/10.35495/AJAB.2020.12.551","url":null,"abstract":"MicroRNAs (miRNAs) are small non-coding RNA molecules, involved in the posttranscriptional gene expression of countless metabolic pathways including plant biomass production. The current work was focused on identification of miRNAs involved in the growth metabolism of Glycine max, Oryza sativa, Zea mays, Sorghum bicolor, Brassica napus, Triticum aestivum. In order to identify conserved miRNA clusters, the miRNA data were collected from miRBase database. Overall, 756, 738, 325, 241, 92, and 125 datasets of the mature miRNA sequences of Glycine max, Oryza sativa, Zea mays, Sorghum bicolor, Brassica napus, Triticum aestivum were collected from miRbase. Using MEGA software, a total of 6, 6, 5, 6, and 3 conserved miRNA clusters were examined in aforementioned crops, respectively, with the aim of studying the conserved miRNA clusters belonging to same gene families. The conserved miRNA clusters were shown to belong to miR166, miR399, miR156, miR171, miR164, miR167, and miR394 families in the selected crops. This study may lead to elucidate the role of these miRNAs and their subsequent exploitation to enhance the biomass production via metabolic pathway engineering.","PeriodicalId":8506,"journal":{"name":"Asian Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2021-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48928795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Performance of Italian ryegrass varieties: Effect of cutting stage on dry matter and total non-structural carbohydrates percentages","authors":"","doi":"10.35495/ajab.2020.02.116","DOIUrl":"https://doi.org/10.35495/ajab.2020.02.116","url":null,"abstract":".","PeriodicalId":8506,"journal":{"name":"Asian Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2021-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41673615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Indika, P. Silva, Makawita, I. Wickramasinghe, I. Wijesekara
{"title":"Using brown seaweed as a biofertilizer in the crop management industry and assessing the nutrient upliftment of crops","authors":"G. Indika, P. Silva, Makawita, I. Wickramasinghe, I. Wijesekara","doi":"10.35495/ajab.2020.04.257","DOIUrl":"https://doi.org/10.35495/ajab.2020.04.257","url":null,"abstract":"Due to the increasing levels of the world population, the demand for agricultural products has also increased over the years. Enhancing the yield and nutrient content of food products is therefore an important aspect in the current context. Experiments were conducted at both lab-scale and field level with extractions from Sargassum sp., to assess the growth of the plant, crop production, and nutrient content of Vigna radiata . A lab-scale experiment was conducted to identify and distinguish the germination percentage and seedling vigor of Sargassum treated and non-treated seeds. This included the application of a concentration series of polysaccharides and a concentration series of crude seaweed extract. The highest germination percentage was observed at 8% polysaccharide content and 15% liquid seaweed extraction (LSE) content resulting in 14% and 8% of germination increment against the control. When increasing the polysaccharide and LSE content exceeding the above-stated values, germination rate and seedling vigor dropped. When applied as foliar applications, an increment of pods against the control showed an increment of 28%, which is the maximum increment reached the 10% LSE concentration. With the polysaccharide application, the maximum yield increment was 31% with 10% concentration. Also, the dry weight content of seeds and the number of pods per plant and seed per pod have increased considerably. Further, both polysaccharide and LSE applications have increased the micronutrient and protein content in seeds. Therefore, it is recommended to use the LSE at 15% concentration and polysaccharide at 8% concentration at the germination stage and as a foliar application to increase the yield and nutrient content of the Vigna radiata plant.","PeriodicalId":8506,"journal":{"name":"Asian Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2021-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44483528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A wheat stress induced WRKY transcription factor TaWRKY32 confers drought stress tolerance in Oryza sativa","authors":"E. Darwish","doi":"10.35495/ajab.2020.06.371","DOIUrl":"https://doi.org/10.35495/ajab.2020.06.371","url":null,"abstract":"WRKY transcription factors group play a significant role in stress signalling pathway. In this study, a WRKY member of bread wheat, TaWRKY , was cloned and its function in response to water stress conditions was studied. Expression analysis revealed that TaWRKY32 mainly expressed when plants were subjected to PEG, NaCl, and cold and probably participate in ABA dependent signalling pathways. This study was done to elucidate the role in transgenic breeding, TaWRK32 was transferred to rice plants under CaMV-35S promoter. Overexpression of TaWRK32 lead to enhanced fresh shoot and root weight at seedling stage when compared to wild type (WT). Moreover, transgenic rice lines under water stress, showed greater recovery rate when compared to WT. Higher values of cell membrane stability and lower Chl a/b ratio of transgenic rice lines under water stress conditions markedly indicated the importance of TaWRKY32 under water stress conditions. These results suggested that TaWRK32 has multifunctional and might be an instrumental tool in breeding for transgenic plants for improved water stress tolerance in wheat.","PeriodicalId":8506,"journal":{"name":"Asian Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2021-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69845685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Predicting biogas production using kinetic model for mesophilic and thermophilic temperature regimes: a theoretical approach","authors":"","doi":"10.35495/ajab.2020.03.196","DOIUrl":"https://doi.org/10.35495/ajab.2020.03.196","url":null,"abstract":"Ever since global warming, largely caused by consumption of fossil fuels, has alarmed the scientists and policy makers, biomass has been gaining acceptance and currency as a means of sustainable energy generation. Biogas generation through anaerobic digestion of biomass is a promising technique of converting carbonaceous material into methane and carbon dioxide i.e major components of biogas. This work aims at determination of methane production rate based on kinetic study while taking into consideration temperature, total solids, volatile solids, residence time, and bacterial growth. We used banana waste including stem, fruit stem, peel and leaves as waste material and applied Chen & Hashimoto kinetic model to measure the methane potential. The wet anaerobic digestion process digested the banana waste inside the bioreactor for 15 days of hydraulic retention time on mesophilic temperature regime 35-40°C and 10 days for thermophilic temperature regime 55-60°C. The methane production was 14.6 m 3 /day using 100 kg/day of banana waste. This showed a close accord to the already observed and published data based on total solid, volatile solid, carbon to nitrogen ratio and temperature.","PeriodicalId":8506,"journal":{"name":"Asian Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2021-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43888110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Recent advances in molecular characterization of Sarcocystis species in some meat producing animals: an updated review","authors":"","doi":"10.35495/ajab.2020.09.502","DOIUrl":"https://doi.org/10.35495/ajab.2020.09.502","url":null,"abstract":"Sarcocystosis is a parasitic disease caused by Sarcocystis species that infect humans and animals. It is prevalent in small ruminants like sheep and goats worldwide and causing pathogenic impacts that lead to economic losses owing to carcass condemnation, abortion, and death. Recently, several molecular and phylogenetic analyses have been developed to differentiate Sarcocystis species including, the 18S rRNA, 28S rRNA, 18S rDNA, and ITS-1 region. In recent years, the mitochondrial cytochrome c oxidase subunit 1 (cox-1) was successfully used for this purpose. The DNA barcoding using the cox 1 gene is a reliable tool to distinguish and identify the main Sarcocystis genotypes. Therefore, several studies confirmed that the cox 1 gene is a promising DNA marker for studying the genus Sarcocystis . The current review aims to highlight the molecular methods that exist for the identification of Sarcocystis species . The results showed that the Sarcocystis species of sheep and goats were genetically close related and may be considered as sibling strains, as well as the cross-infection may happen among them. Consequently, the host specificity of several Sarcocystis species is questionable. The findings additional emphasized that experimental transmission investigations within the proposed definitive host are required to confirm the characteristics and host ranges of the Sarcocystis spp. in sheep and goats. The current review represents updated knowledge about molecular discrimination of Sarcocystis species in small ruminants by reviewing and analyzing the recent articles in this aspect.","PeriodicalId":8506,"journal":{"name":"Asian Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2021-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49644633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of organic fertilization on quality and yield of oriental tobacco (Nicotiana tabacum L.) under Mediterranean conditions","authors":"Ioanna Tabaxi","doi":"10.35495/ajab.2020.05.274","DOIUrl":"https://doi.org/10.35495/ajab.2020.05.274","url":null,"abstract":"Greece is a country that primarily cultivates tobacco. Over the years and the increase of inputs to conventional tobacco cultivation and the groundwater nitrate pollution, producers and industry began to look at organic farming. However, for the field crop of tobacco knowledge is limited to inorganic fertilization only and it is necessary now to find out the effect of organic fertilization on soil, yield and quality of tobacco. Therefore, two-year field experiments were carried out during 2017-2018 in Agricultural University of Athens. Three oriental tobacco varieties were cultivated (Basmas Xanthi 81, Katerini S53 and Myrodata Agriniou 30Α) in split-plot design, with four fertilization treatments (Control, Compost, Manure and NPK). According to the results, the yield was significantly affected only by the variety and the highest yield was 3.488 (kg ha-1) in Myrodata Agriniou variety. The plant height was significantly affected by variety and ranged from 110,77 cm (Basmas Xanthi) to 121.42 cm (Myrodata Agriniou). The number of leaves was significantly affected only by the variety; Katerini S53 had the highest number. The nicotine content was significantly affected by variety and year and ranged between 2.52% (Myrodata Agriniou) and 4.22 % (Basmas Xanthi). As nicotine increases, sugars finally decrease with the application of organic fertilizers. Summarizing, most of the soil characteristics significantly affected by the fertilization and showed generally improvement. The plant growth and the final yield was determined by the variety. With organic tobacco cultivation, the quality and yields of oriental varieties have improved significantly.","PeriodicalId":8506,"journal":{"name":"Asian Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2021-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48624926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Nutritional and physical requirements for mycelial growth and basidiocarp production of Trametes elegans from the Philippines","authors":"R. M. Dulay","doi":"10.35495/ajab.2020.06.339","DOIUrl":"https://doi.org/10.35495/ajab.2020.06.339","url":null,"abstract":"This paper highlights the rescue and optimal cultural conditions of the naturally-occurring Trametes elegans . This mushroom has white fan-like leathery fruiting body that commonly grows singly on deadwood. The secondary mycelial culture has off-white, velvety texture","PeriodicalId":8506,"journal":{"name":"Asian Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2021-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69845968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Exposure to heavy metals causes histopathological changes and alters antioxidant enzymes in fresh water fish (Oreochromis niloticus)","authors":"Tuba Rehman","doi":"10.35495/ajab.2020.03.143","DOIUrl":"https://doi.org/10.35495/ajab.2020.03.143","url":null,"abstract":"Expansion in technology, development in industries and extensive applications of synthetic chemical have become major regulatory issues toward the public health, wildlife and aquatic ecosystems. Therefore, the monitoring and investigation of environmental contaminants in order to mitigate the adverse impacts is crucial to save public health and aquatic wildlife. The present study describes the deleterious effects of heavy metals at low levels in fresh water fish. A total of 120 fish of same age and body mass were arbitrarily distributed and allocated into eight groups (A-H). Group A was run parallel to other groups and maintained as control group. Collected data was analyzed by using Tukey’s Test. Results indicated various clinical ailments including erratic swimming and jerking movement. Microscopic observation of gills, liver and brain tissues showed different histopathological changes in fish exposed to various heavy metals. The antioxidant enzymes activities were increased or decreased in a concentration and enzyme dependent manner. Exposure of treatments showed significant increase in peroxidase and glutathione-s-transferase but decreased catalase and superoxide dismutase activity at higher exposure to heavy metals. Regarding overall toxicity, nickel was least sensitive towards the fish, but tertiary metal mixture was more toxic to fish (Oreochromis niloticus) followed by binary mixture. In conclusion, results of our study suggested that the exposure to different heavy metals even at low concentrations poses serious concern towards their potential danger to the survival and growth of fish and induces deleterious effects in gills, brain and liver tissues.","PeriodicalId":8506,"journal":{"name":"Asian Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2021-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49533155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}