Korean journal of biological sciences最新文献_第9页

The effect of cortisol on proliferative properties of flounder (Paralychthys Olivaceus) B Lymphocytes 皮质醇对比目鱼B淋巴细胞增殖特性的影响

Korean journal of biological sciences Pub Date : 2003-01-01 DOI: 10.1080/12265071.2003.9647683

Sang-hoon Choi, C. Oh

{"title":"The effect of cortisol on proliferative properties of flounder (Paralychthys Olivaceus) B Lymphocytes","authors":"Sang-hoon Choi, C. Oh","doi":"10.1080/12265071.2003.9647683","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647683","url":null,"abstract":"Flounder B lymphocytes isolated from different tissues were studied in terms of cell proliferation, apoptosis and the effects of cortisol on these processes. B lymphocytes, isolated from the flounder head kidney and spleen, were characterized by higher proliferation and lower intracellular calcium (Ca2*i) response to Ig‐crosslinking compared with peripheral blood B lymphocytes. Cortisol induced high levels of apoptosis (150% of control levels) in peripheral blood B lymphocytes, in combination with a stimulatory LPS signal. Head kidney and to a lesser extent spleen B lymphocytes, although less sensitive than their equivalent in peripheral blood, underwent cortisol‐induced apoptosis irrespective of extra stimulation up to 142% of control levels. Also proliferation with and without LPS stimulation was suppressed by cortisol (compared to plasma values measured during stress conditions) that is effective in inducing a significant increase in apoptosis in all three populations of B‐cells, suggesting that cortisol may be important for immunoregulation in both stressed and non‐stressed conditions. This implies possible severe impact of stress on lymphocyte development and activity. Different sensitivity of B‐cells to the corticosteroid, with respect to developmental stage and activity, may prevent excessive and long lasting depletion of B‐lymphocytes.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"57 - 62"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647683","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Screening of cDNAs encoding secreted and membrane proteins in the nervous system of marine snail Aplysia kurodai 海蜗牛神经系统分泌蛋白和膜蛋白编码cdna的筛选

Korean journal of biological sciences Pub Date : 2003-01-01 DOI: 10.1080/12265071.2003.9647695

Min-Jeong Kim, D. Chang, C. Lim, W. Park, B. Kaang

引用次数: 0

Biochemical characterization of a putative calcium influx factor as a diffusible messenger in Jurkat cells, Xenopus oocytes, and yeast 在Jurkat细胞、爪蟾卵母细胞和酵母中作为扩散信使的假定钙内流因子的生化特性

Korean journal of biological sciences Pub Date : 2003-01-01 DOI: 10.1080/12265071.2003.9647686

Hak Yong Kim

{"title":"Biochemical characterization of a putative calcium influx factor as a diffusible messenger in Jurkat cells, Xenopus oocytes, and yeast","authors":"Hak Yong Kim","doi":"10.1080/12265071.2003.9647686","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647686","url":null,"abstract":"Highly purified high performance thin layer chromatography (HPTLC) fractions containing a putative calcium influx factor (CIF) were prepared from the Jurkat cells and Xenopus oocytes in which Ca2+ stores were depleted by thapsigargin treatment and from the yeast in which intracellular Ca2+ stores were also depleted by genetic means. Micro injection of the fractions has been shown to elicit Ca2+‐dependent currents in Xenopus oocytes. The nature of the membrane currents evoked by the putative CIF appeared to be carried by chloride ions since the current was blocked by the selective chloride channel blocker 1 mM niflumic acid and its reversal potential was about ‐24 mV. Injection of the calcium chelator 1,2‐bis(2‐aminophenoxy)ethane‐N, N,N’,N‘‐tetraacetic acid (BAPTA) eradicated the current activities, suggesting the current responses are entirely Ca2+‐dependent. Moreover, the currents were sensitive to the removal of extracellular calcium, indicating the dependence on calcium entry through the plasma membrane calcium entry channels. CIF activities were insensitive to protease, heat, and acid treatments and to Dische‐reaction whereas the activities were sensitive to nucleotide pyrophosphatase and hydrazynolysis. The fraction might have a sugar because it was sensitive to Molisch test and Seliwaniff's resorcinol reaction. From the above results, CIF as a small and stable molecule seems to have pyrimidine, pyrophosphate, and a sugar moiety.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"75 - 79"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647686","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution pattern of white snakeroot as an invasive alien plant and restoration strategy to inhibit its expansion in Seoripool park, Seoul 外来入侵植物白蛇根的分布格局及抑制其扩张的恢复策略

Korean journal of biological sciences Pub Date : 2003-01-01 DOI: 10.1080/12265071.2003.9647705

Hansol Lee, Hae‐Mi Yoo, Chang-Seok Lee

{"title":"Distribution pattern of white snakeroot as an invasive alien plant and restoration strategy to inhibit its expansion in Seoripool park, Seoul","authors":"Hansol Lee, Hae‐Mi Yoo, Chang-Seok Lee","doi":"10.1080/12265071.2003.9647705","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647705","url":null,"abstract":"White snakeroot (Ageratina altissima (L.) R. King & H. Robinson) as an invasive alien plant appeared more abundantly at lower elevations where frequent artificial interferences prevailed than at higher elevations where such impacts were less. They appeared abundantly in introduced forests such as black locust plantation but they did not appear or were rare in natural forests such as oak forest. But an exceptional phenomenon where white snakeroot did not appear was found in a Korean pine stand with dense cover afforested recently. Appearance status of white snakeroot in each section of trampling path depended on breadth of the path and relative light intensity. Growth of white snakeroot measured as the number of ramet per genet, height, and biomass was better near the trampling path and was reduced toward the forest interior. The growth was proportionate to the relative light intensity measured according to distance from the trampling path. Such results support the fact generally known in relation to invasion and expansion of the invasive alien plants. From this viewpoint, we suggest a management plan that applies ecological restoration principles to address ecosystems infected with white snakeroot by restoring the integral feature of the degraded nature and more thoroughly conserving the remaining nature.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"197 - 205"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647705","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Organization of projections from the medial temporal cortical areas to the ventral striatum in macaque monkeys 猕猴内侧颞皮质区到腹侧纹状体的投射组织

Korean journal of biological sciences Pub Date : 2003-01-01 DOI: 10.1080/12265071.2003.9647710

Y. Jung, Sungwon J. Hong

{"title":"Organization of projections from the medial temporal cortical areas to the ventral striatum in macaque monkeys","authors":"Y. Jung, Sungwon J. Hong","doi":"10.1080/12265071.2003.9647710","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647710","url":null,"abstract":"Recent evidence on behaviors in macaque monkeys indicate that the medial temporal cortical areas such as the entorhinal cortex (EC), perirhinal cortex, and parahippocampal cortex (PHC) are importantly involved in limbic and sensory memory function. Neuroanatomical studies also have demonstrated that the medial temporal cortical areas are connected with the ventral striatum, although comparatively little is known about the precise topography of these connections. We investigated the topographic organization of connections between the medial temporal cortical areas and the ventral striatum by placing retrograde tracers into five different regions of the ventral striatum: the ventromedial caudate nucleus, ventral shell, central shell, dorsal core of the nucleus accumbens (NA), and ventrolateral putamen. We found that the shell of the NA was the main projection site from the medial temporal cortical areas. Within the shell of the NA, there were also differential connections: EC diffusely innervates shell of the NA, while the projections from the perirhinal cortex and PHC concentrate on the ventral shell of the NA. Taken together, it is possible that the ventral shell of the NA is the main integration site of the limbic and sensory memory coming from the EC, perirhinal cortex, and PHC.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"237 - 248"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647710","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Flavonoids from leaves and exocarps of the grape Kyoho 葡萄叶和外果皮中的黄酮类化合物

Korean journal of biological sciences Pub Date : 2003-01-01 DOI: 10.1080/12265071.2003.9647723

Hye-Jeong Park, Hyeon-cheol Cha

引用次数: 35

Alteration in magnesium level in acute myocardial infarction 急性心肌梗死时镁水平的改变

Korean journal of biological sciences Pub Date : 2003-01-01 DOI: 10.1080/12265071.2003.9647701

T. Angeline, K. Ramadevi, R. Aruna, G. Mohan, N. Jeyaraj

{"title":"Alteration in magnesium level in acute myocardial infarction","authors":"T. Angeline, K. Ramadevi, R. Aruna, G. Mohan, N. Jeyaraj","doi":"10.1080/12265071.2003.9647701","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647701","url":null,"abstract":"Sodium, potassium, calcium, zinc and magnesium levels in the serum of 31 patients diagnosed as acute myocardial infarction were analyzed on admission (within 24 Hours) and after 48 hours. The results were compared with those of 26 age matched controls. No significant difference was observed in the mean sodium, potassium, calcium and zinc levels between the cases and controls. Compared to the controls, however, the variation in the level of magnesium is highly significant at the time of admission as well as after 48 hours. When the risk factors like diabetes mellitus, hypertension, smoking and alcohol were considered, it is found that there is no significant difference between the risk groups as well as between the patients. The alteration in magnesium level in acute myocardial infarction is independent of these risk factors. Within the first 24 hours, the significant decrease in serum magnesium (35–51% fall when compared with the control group), correlates with its entry into the cell following ischemia. From this hypo‐magnesemic state, it rises to 9–22 times after 48 hours. This hyper‐magnesemia after 48 hours is probably due to the shift of magnesuim from the intracellular fluid compartment to the extracellular fluid compartment that follows cellular recovery. Therefore, including magnesium in the immediate management of acute myocardial infarction will be beneficial in the early recovery.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"169 - 171"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647701","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cadmium‐induced gene expression is regulated by MTF‐1, a key metal‐responsive transcription factor 镉诱导的基因表达受MTF - 1调控，MTF - 1是一种关键的金属响应转录因子

Korean journal of biological sciences Pub Date : 2003-01-01 DOI: 10.1080/12265071.2003.9647702

Ronojoy Sen Gupta, J. Ahnn

{"title":"Cadmium‐induced gene expression is regulated by MTF‐1, a key metal‐responsive transcription factor","authors":"Ronojoy Sen Gupta, J. Ahnn","doi":"10.1080/12265071.2003.9647702","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647702","url":null,"abstract":"The transition metal cadmium is a serious occupational and environmental toxin. To inhibit cadmium‐induced damage, cells respond by increasing the expression of genes that encode stress‐responsive proteins. The metal‐regulatory transcription factor 1 (MTF‐1) is a key regulator of heavy‐metal induced transcription of metallothionein‐I and II and other genes in mammals and other metazoans. Transcriptional activation of genes by MTF‐1 is mediated through binding to metal‐responsive elements in the target gene promoters. Phosphorylation of MTF‐1 plays a critical role in the cadmium‐inducible transcriptional activation of metallothionein and other responses. Studies using inhibitors indicate that multiple kinases and signal transduction cascades, including those mediated by protein kinase C, tyrosine kinase and casein kinase II, are essential for cadmium‐mediated transcriptional activation. In addition, calcium signaling is also involved in regulating metal‐activated transcription. In several species, cadmium induces heat shock genes. Recently much progress has been made in elucidating the cellular machinery that regulates this metal‐inducible gene expression. This review summarizes these recent advances in understanding the role of some known cadmium‐responsive genes and the molecular mechanisms that activate metal‐responsive transcription factor, MTF‐1.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"173 - 186"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647702","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59656008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Promoter structure and transcriptional activity of human complement receptor type I (CR1) gene 人补体受体ⅰ型(CR1)基因启动子结构及转录活性

Korean journal of biological sciences Pub Date : 2003-01-01 DOI: 10.1080/12265071.2003.9647684

J. H. Kim, Young Ju Lee, Juyeon Nam, H. Shim, S. Choe

引用次数: 1

Morphological differences of immature stages between males and females in a Korean wood‐feeding cockroach (cryptocercus kyebangensis) 韩国木食蜚蠊(cryptocercus kyebangensis)雌雄未成熟阶段的形态差异

Korean journal of biological sciences Pub Date : 2003-01-01 DOI: 10.1080/12265071.2003.9647690

Y. Park, J. Choe

引用次数: 4