Korean journal of biological sciences最新文献_第8页

Distribution and morphology of calretinin‐immunoreactive neurons in the intermediate and deep layers of cat superior colliculus 猫上丘中层和深层calretinin免疫反应神经元的分布和形态

Korean journal of biological sciences Pub Date : 2003-01-01 DOI: 10.1080/12265071.2003.9647698

C. Jeon, Jin-Young Sung, S. Hong

{"title":"Distribution and morphology of calretinin‐immunoreactive neurons in the intermediate and deep layers of cat superior colliculus","authors":"C. Jeon, Jin-Young Sung, S. Hong","doi":"10.1080/12265071.2003.9647698","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647698","url":null,"abstract":"Calretinin is thought to play roles in calcium buffering. Its site of expression has been extensively studied in the central nervous system. We previously reported (Hong et al., 2002, Neurosci. Res., 44:325–335) calretinin expression in the superficial layers of the cat superior colliculus (SC). In the present study, we studied the distribution of calretinin in the intermediate and deep layers by immunocytochemistry. We found striking differences in calretinin immunoreactivity among the superficial, intermediate, and deep layers. In contrast to the superficial layers, the intermediate and deep layers contained many calretinin‐immunoreactive (IR) neurons. They formed two laminar tiers. The first tier, which was very distinctive, was found within the upper intermediate gray layers and formed clusters of labeled neurons in many sections. The second tier of calretinin‐IR neurons was found in the deep gray layer. However, the second tier was not distinctive compared to the first tier and the labeled neurons did not form any clusters. Calretinin‐IR neurons in the intermediate and deep layers varied dramatically in morphology and included vertical fusiform, pyriform, and stellate neurons. Neurons with varicose dendrites were also labeled. Most of the labeled neurons were small to medium in size. Enucleation appeared to have no effect on the distribution of calretinin immunoreactivity in the contralateral intermediate and deep layers of the SC. The results indicate that calretinin is present in various neurons, at different locations. These results should be applicable for better understanding of the functional organization of the SC.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"151 - 157"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647698","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chitosan increases the release of renal dipeptidase from porcine renal proximal tubule cells 壳聚糖增加猪肾近端小管细胞肾二肽酶的释放

Korean journal of biological sciences Pub Date : 2003-01-01 DOI: 10.1080/12265071.2003.9647721

H. Yoon, Y. H. Kim, Sung Wook Park, Hwanghee-Blaise Lee, H. Park

{"title":"Chitosan increases the release of renal dipeptidase from porcine renal proximal tubule cells","authors":"H. Yoon, Y. H. Kim, Sung Wook Park, Hwanghee-Blaise Lee, H. Park","doi":"10.1080/12265071.2003.9647721","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647721","url":null,"abstract":"Renal dipeptidase (RDPase, membrane dipeptidase, dehydropeptidase 1, EC 3.4.13.19) has been widely studied since it was first purified from porcine kidney brush border membrane. It was reported that RDPase activity in urine samples of acute and chronic renal failure patients decreases. Nitric oxide (NO) is a highly reactive free radical involved in a number of physiological and pathological processes. NO is able to act in a dual mode, leading either to induction of apoptosis or to blunted execution of programmed cell death. NO inhibited the RDPase release from porcine renal proximal tubules, which could be blocked by L‐NAME. Chitosan, the linear polymer of D‐glucosamine in β(1?4) linkage, not only reversed the decreased RDPase release by NO but also increased NO production in the proximal tubule cells. The stimulatory effect of NO on RDPase release from proximal tubules in the presence of chitosan must be different from the previously proposed mechanism of RDPase release via NO signaling pathway. Chitosan stimulated the RDPase release in the proximal tubules and increased RDPase activity to 220% and 250% at 0.1% and 1%, respectively. RDPase release was decreased to about 40% in the injured proximal tubules and was recovered in proportion to the increase of chitosan. Chitosan may be useful in recovery of renal function from HgCl2 injury.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"309 - 315"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647721","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Effects of Acute Acid Stress on Hatching and Mortality of Hermaphroditic Teleost, Rivulus marmoratus (Cyprinodontiformes; Rivulidae) 急性酸胁迫对雌雄同体硬骨鱼孵化及死亡率的影响Rivulidae)

Korean journal of biological sciences Pub Date : 2003-01-01 DOI: 10.1080/12265071.2003.9647726

A. Kim, Meoung‐Sook Lee, E. Park

引用次数: 3

A new species of the genus hymeniacidon (Demospongiae: Halichondrida: Halichondriidae) from Korea 文章标题韩国膜螨属一新种(绵螨科:绢螨科)

Korean journal of biological sciences Pub Date : 2003-01-01 DOI: 10.1080/12265071.2003.9647703

C. Sim, K. Lee

引用次数: 6

Difference of early experience of foals according to dominance ranks of their mothers in the Jeju pony (Equus caballus) 母马优势等级对小马驹早期经验的影响

Korean journal of biological sciences Pub Date : 2003-01-01 DOI: 10.1080/12265071.2003.9647692

J. Rho, J. Choe

引用次数: 0

Trigger factor interacts with DnaA protein to stimulate its interaction with DnaA box 触发因子与dna蛋白相互作用，刺激其与dna盒子相互作用

Korean journal of biological sciences Pub Date : 2003-01-01 DOI: 10.1080/12265071.2003.9647687

Yong Sun Lee, June-Chul Lee, H. Kim, Sukhyun Kang, Joo Seok Han, J. B. Kim, D. Hwang

引用次数: 0

Life history and population dynamics of Korean woodroach (Cryptocercus kyebangensis) populations 韩国木泥鳅(Cryptocercus kyebangensis)种群生活史和种群动态

Korean journal of biological sciences Pub Date : 2003-01-01 DOI: 10.1080/12265071.2003.9647691

Y. Park, J. Choe

引用次数: 2

Structural characterization for N‐terminal domain of caveolin‐1 caveolin - 1 N末端结构域的结构表征

Korean journal of biological sciences Pub Date : 2003-01-01 DOI: 10.1080/12265071.2003.9647706

Jongmin Kim, Jaeyoung Shin, Heonyong Park

{"title":"Structural characterization for N‐terminal domain of caveolin‐1","authors":"Jongmin Kim, Jaeyoung Shin, Heonyong Park","doi":"10.1080/12265071.2003.9647706","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647706","url":null,"abstract":"Caveolin‐1 is a principal protein in the plasma membrane microdomains called caveolae. Caveolae play an important role in the transcytosis and pinocytosis. Therefore, caveolin‐1 is most likely to work for the membrane dynamic events. In addition, caveolin‐1 interacts with various signaling molecules. Although caveolin‐1 possesses a variety of physiological functions, its structural properties were little construed. Here we analyzed the structural dynamics of the N‐terminal caveolin‐1 (residues 1–101), in order to better understand the structural properties in terms of its versatile functionality. We first analyzed its oligomeric form using GST‐fused N‐terminal domain, revealing that it equilibrates between a dimer and monomers in a concentration‐dependent manner. The N‐terminal domain of caveolin‐1 was previously found to form a heptamer, so that our data suggest the dimeric form as an intermediate structure for the heptamer formation. Then, we obtained the folding profile, which indicated that AGH2o is about 0.5 ±0.03 kcal/mol. The stability of N‐terminal domain is relatively low, indicating that N‐terminal domain may not be crystalline. Conclusively, the dynamic and flexible structure of N‐terminal domain appears more favorable to maintain the versatile functions of caveolin‐1.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"207 - 211"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647706","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Cellular changes of phenotype and collagenase‐1 expression in healing corneal stromal cells 愈合后角膜基质细胞表型和胶原酶- 1表达的细胞变化

Korean journal of biological sciences Pub Date : 2003-01-01 DOI: 10.1080/12265071.2003.9647715

Jae‐Chang Jung

{"title":"Cellular changes of phenotype and collagenase‐1 expression in healing corneal stromal cells","authors":"Jae‐Chang Jung","doi":"10.1080/12265071.2003.9647715","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647715","url":null,"abstract":"Matrix metalloproteinases (MMPs) have been implicated in corneal diseases of the eye. Among the MMPs, collagenase‐1 (MMP‐1) participates in remodeling the extracellular matrix of corneal stroma during wound healing. Here the temporal expression patterns of both IL‐1α and collagenase‐1 were examined during regenerative corneal wound healing. IL‐1α was highly expressed in the healing epithelium at 3 d after epithelial abrasion wound, thereafter its expression returned to normal level. Primary cultured quiescent stromal cells (keratocytes) from uninjured normal cornea were round‐shaped and did not expresse collagenase‐1. However, freshly‐cultured stromal cells isolated 3d after epithelial abrasion wound (abrasion wound) underwent marked morphological changes, and a certain percentage of cell populations was transformed into fibroblasts. By immunofluorescence, collagenase‐1 expression was detected only in spindle‐shaped fibroblasts. Interestingly, most freshly‐isolated cells from remodeling corneal repair tissue 10 d after abrasion injury were found to have fibroblastic morphology, and also constitutively expressed a high level of collagenase‐1 in vitro primary culture. However, its expression was not found in the cells isolated 14 d after wound and cells seemed to have returned to normal quiescent stromal cells. Taken together, these results suggest that differentially expressed IL‐1α in the epithelium might play important roles in wound healing, and important signalling pathways are altered as cells take on the fibroblastic morphology.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"271 - 277"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647715","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening of cDNAs encoding secreted and membrane proteins in the nervous system of marine snail Aplysia kurodai 海蜗牛神经系统分泌蛋白和膜蛋白编码cdna的筛选

Korean journal of biological sciences Pub Date : 2003-01-01 DOI: 10.1080/12265071.2003.9647695

Min-Jeong Kim, D. Chang, C. Lim, W. Park, B. Kaang

引用次数: 0