Korean journal of biological sciences最新文献

Six SIGMA evaluation of 17 biochemistry parameters using bias calculated from internal quality control and external quality assurance data. 利用内部质量控制和外部质量保证数据计算出的偏差，对 17 个生化参数进行六标法评估。

IF 2.5

Korean journal of biological sciences Pub Date : 2024-01-25 DOI: 10.5937/jomb0-43052

Tülay Çevlik, Goncagül Haklar

{"title":"Six SIGMA evaluation of 17 biochemistry parameters using bias calculated from internal quality control and external quality assurance data.","authors":"Tülay Çevlik, Goncagül Haklar","doi":"10.5937/jomb0-43052","DOIUrl":"10.5937/jomb0-43052","url":null,"abstract":"Background: Six Sigma is a popular quality management system that enables continuous monitoring and improvement of analytical performance in the clinical laboratory. We aimed to calculate sigma metrics and quality goal index (QGI) for 17 biochemical analytes and compare the use of bias from internal quality control (IQC) and external quality assurance (EQA) data in the calculation of sigma metrics.Methods: This retrospective study was conducted in Marmara University Pendik E&R Hospital Biochemistry Laboratory. Sigma metrics calculation was performed as (TEa-bias)/CV). CV was calculated from IQC data from June 2018 - February 2019. EQA bias was calculated as the mean of % deviation from the peer group means in the last seven surveys, and IQC bias was calculated as (laboratory control result mean-manufacturer control mean)/ manufacturer control mean) x100. In parameters where sigma metrics were <5; QGI=bias/1.5 CV) score of <0.8 indicated imprecision, >1.2 pointed inaccuracy, and 0.8-1.2 showed both imprecision and inaccuracy.Results: Creatine kinase (both levels), iron and magnesium (pathologic levels) showed an ideal performance with ≥6 sigma level for both bias determinations. Eight of the 17 parameters had different sigma levels when we compared sigma values calculated from EQA and IQC derived bias% while the rest were grouped at the same levels.Conclusions: Sigma metrics is a good quality tool to assess a laboratory's analytical performance and facilitate the comparison of the assay performances in the same manner across multiple systems. However, we might need to design a tight internal quality control protocol for analytes showing poor assay performance.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"43-49"},"PeriodicalIF":2.5,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10943459/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81677584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Ku70/Ku80 as ADD1/SREBP1c interacting proteins Ku70/Ku80为ADD1/SREBP1c互作蛋白的鉴定

Korean journal of biological sciences Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647733

Y. S. Lee, Hae‐Young Koh, S. Park, J. B. Kim

{"title":"Identification of Ku70/Ku80 as ADD1/SREBP1c interacting proteins","authors":"Y. S. Lee, Hae‐Young Koh, S. Park, J. B. Kim","doi":"10.1080/12265071.2004.9647733","DOIUrl":"https://doi.org/10.1080/12265071.2004.9647733","url":null,"abstract":"In vertebrates, multisubunit cofactors regulate gene expression through interacting with cell‐type‐ and gene‐specific DNA‐binding proteins in a chromatin‐selective manner. ADD1/SREBP1c regulates fatty acid metabolism and insulin‐dependent gene expression through binding to SRE and E‐box motif with dual DNA binding specificity. Although its transcriptional and post‐translational regulation has been extensively studied, its regulation by interacting proteins is not well understood. To identify cellular proteins that associate with nuclear form of ADD1/SREBP1c, we employed the GST pull‐down system with Hela cell nuclei extract. In this study, we demonstrated that Ku proteins interact specifically with ADD1/SREBP1c protein. GST pull‐down combined with peptide sequencing analysis revealed that Ku80 binds to ADD1/SREBP1c in vitro. Additionally, western blot analysis showed that Ku70, a heterodimerizing partner of Ku80, also associates with ADD1/SREBP1c. Furthermore, co‐transfection of Ku70/Ku80 with ADD1/SREBP1c enhanced the transcriptional activity of ADD1/SREBP1c. Taken together, these results suggest that the Ku proteins might be involved in the lipogenic and/or adipogenic gene expression through interacting with ADD1/SREBP1c.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"8 1","pages":"49 - 55"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2004.9647733","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Three new species of collocheres (copepoda, siphonostomatoida, asterocheridae) associated with crinoids and ophiuroids from Korea 标题韩国菊科和蛇科菊科三新种(桡足目、单足目、星足科)

Korean journal of biological sciences Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647760

Sook Shin, Il-Hoi Kim

引用次数: 1

Ventral striatal connections of unimodal and multimodal cortex of the superior temporal sulcus in macaque monkeys(macacca nemestrina) 猕猴颞上沟皮层单峰和多峰的腹侧纹状体连接

Korean journal of biological sciences Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647767

Y. Jung, Sungwon J. Hong

{"title":"Ventral striatal connections of unimodal and multimodal cortex of the superior temporal sulcus in macaque monkeys(macacca nemestrina)","authors":"Y. Jung, Sungwon J. Hong","doi":"10.1080/12265071.2004.9647767","DOIUrl":"https://doi.org/10.1080/12265071.2004.9647767","url":null,"abstract":"Extrinsic connections between the cortex of the superior temporal sulcus (STS) and the ventral striatum in pigtail macaque monkeys (Macacca nemestrina) were studied by injection of retrograde tracers into the ventromedial caudate nucleus, the ventral and central shells of the nucleus accumbens (NA), the dorsal core of the NA, and the ventrolateral putamen. In the present study, we demonstrate that the projections from the unimodal (area TAa, IPa, TEa, and TEm) and the multimodal (area TPO and PGa) sensory association areas in the STS mainly terminate in the ventromedial caudate nucleus as well as in the ventral and central shells of the NA. However, there are only few projections to the dorsal core of the NA and the ventrolateral putamen from the sensory association cortex in the STS. Based on these differential neural connections between the subterritories of the ventral striatum and the sensory association areas, the ventromedial caudate nucleus and the shells of NA appear to be major integration sites for sensory input from the STS and functionally different from the dorsal core of NA and the ventrolateral putamen.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"8 1","pages":"319 - 328"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2004.9647767","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59656640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Tra2α and hnRNP K might be functional partners of Rbm for regulation of RNA processes during spermatogenesis Tra2α和hnRNP K可能是Rbm在精子发生过程中调控RNA过程的功能伙伴

Korean journal of biological sciences Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647735

Jungmin Lee, Euisu Kim, S. Jang, K. Rhee

引用次数: 1

Copepodid stages of Ergasilus hypomesi Yamaguti (Copepoda, Poecilostomatoida, Ergasilidae) from a brackish lake in Korea 韩国某咸淡湖泊中拟沙螽桡足期(桡足目，拟沙螽科，拟沙螽科)

Korean journal of biological sciences Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647727

Il-Hoi Kim

引用次数: 5

Taxonomic notes on some Korean species of the genus ampelisco (amphipoda, ampeliscidae) 韩国蛇足属若干种的分类学注释(片足目，蛇足科)

Korean journal of biological sciences Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647759

Young Hyo Kim, Y. Eun, Kyung-Sook Lee

引用次数: 1

Effect of host egg color dimorphism on interactions between the vinous‐throated parrotbill (Paradoxornis webbianus) and common cuckoo (Cuculus canorus) 寄主卵颜色二态性对毒喉鹦鹉与普通杜鹃相互作用的影响

Korean journal of biological sciences Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647737

Jin‐Won Lee, Jeong-Chil Yoo

{"title":"Effect of host egg color dimorphism on interactions between the vinous‐throated parrotbill (Paradoxornis webbianus) and common cuckoo (Cuculus canorus)","authors":"Jin‐Won Lee, Jeong-Chil Yoo","doi":"10.1080/12265071.2004.9647737","DOIUrl":"https://doi.org/10.1080/12265071.2004.9647737","url":null,"abstract":"To investigate the effect of host egg color dimorphism on the vinous‐throated parrotbill (Paradoxornis webbianus) – common cuckoo (Cuculus canorus) interactions, we monitored breeding nests of vinous‐throated parrotbills, and conducted model egg experiments, using two colors: white and blue. Of the 190 nests examined in this study, cuckoo parasitism occurred at 10 nests (8 blue and 2 white egg clutches, respectively), and only blue cuckoo eggs were found. This frequency was similar to the egg‐color ratio of all host nests found (151 blue and 39 white egg clutches). Vinous‐throated parrotbills showed high rejection rate towards both cuckoo eggs and model ones. There was a significant difference in rejection rates towards mimetic (blue) and non‐mimetic (white) eggs in blue egg clutches. Mimetic eggs put in nests took significantly longer to be rejected than non‐mimetic ones. The most common rejection method used by the hosts was egg ejection (puncture‐ejection). The costs of ejecting non‐mimetic eggs tended to be lower than those of ejecting mimetic eggs. These results indicate that egg‐color dimorphism in this species favors the individuals having white egg clutches in terms of higher rejection rate and lower ejection costs of the parasitic eggs. This study also suggests that egg‐color dimorphism of the vinous‐throated parrotbill decreases the effect of cuckoo parasitism on host populations.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"8 1","pages":"77 - 80"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2004.9647737","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59656331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Relationships between methane production and sulfate reduction in reclaimed rice field soils 复垦稻田土壤甲烷产量与硫酸盐还原的关系

Korean journal of biological sciences Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647761

Ju-hwan Lee, Kang-Hyun Cho

{"title":"Relationships between methane production and sulfate reduction in reclaimed rice field soils","authors":"Ju-hwan Lee, Kang-Hyun Cho","doi":"10.1080/12265071.2004.9647761","DOIUrl":"https://doi.org/10.1080/12265071.2004.9647761","url":null,"abstract":"The change in relationships between methane production and sulfate reduction was investigated in reclaimed rice field soils at different time points after reclamation of tidal flat in Korea. Sulfate concentrations of soils in the ca. 60‐year‐old and 26‐year‐old reclaimed rice fields were much lower than that in a natural tidal flat. During 60 d of anaerobic incubation, total methane production and sulfate consumption of the soil slurries were 7.0 μmol CH4/g and 8.2 μnol SO4 2‐/g in the 60‐year‐old rice field, 5.6 μmol CH4/g and 12.7 μmol SO4 2‐/g in the 26‐year‐old rice field, and ca. 0 μmol CH4/g and 22.4 μmol SO4 2‐/g in a natural tidal flat. Relative percent electron flow through sulfate reduction in the 60‐year‐old rice field was much lower (50.8%) compared with the 26‐year‐old rice field (69.3%) and the tidal flat (99.9%). The addition of an inhibitor of methanogenesis (2‐bromoethanesulfonate) had no effect on sulfate reduction in the soil slurries of the reclaimed rice fields. However, instant stimulation of methane production was achieved with addition of an inhibitor of sulfate reduction (molybdate) in the soil slurries from the 26‐year‐old reclaimed rice field. The specific inhibitor experiments suggest that the relationship of methanogenesis and sulfate reduction might become mutually exclusive or syntrophic depending on sulfate content in the soil after reclamation. Sulfate, thus sulfate reduction activity of sulfate‐reducing bacteria, would be an important environmental factor that inhibits methane production and determines the major pathway of electron and carbon flow in anaerobic carbon mineralization of reclaimed rice field soils.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"8 1","pages":"281 - 288"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2004.9647761","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59656446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reproductive cycle and spawning rhythm of the ascidian, Halocynthia hilgendorfi ritteri 海鞘的生殖周期和产卵节律

Korean journal of biological sciences Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647731

Young-jin Choi, Chi‐Hoon Lee, S. Rho, Young-Don Lee

{"title":"Reproductive cycle and spawning rhythm of the ascidian, Halocynthia hilgendorfi ritteri","authors":"Young-jin Choi, Chi‐Hoon Lee, S. Rho, Young-Don Lee","doi":"10.1080/12265071.2004.9647731","DOIUrl":"https://doi.org/10.1080/12265071.2004.9647731","url":null,"abstract":"Reproductive cycle and spawning rhythm with lunar cycle of the ascidian, Halocynthia hilgendorfi ritteri were investigated by histological examination. The specimens were sampled in the coastal waters of Yongdam, northwest of Jeju Island, Korea, from November 2001 to January 2003. H. hilgendorfi ritteri is a synchronous hermaphrodite; the gonads are located in the mantle. The reproductive cycle can be grouped into the following successive stages in the ovary: growth (February to June), vitellogenesis (April to September), mature (July to December), spent (November to February), and recovery (December to April). Likewise, in the testis, the stages observed were: growth (October), mature (October to December), spent (November to February), and resting (January to September). Major spawning probably occurs between November and January, when water temperatures decrease. The histological observations of the gonads suggested that this species is a multiple spawner during the spawning period. Spawning occurred between the new moon and full moon, and again between the full moon and new moon, suggesting that the spawning rhythm is influenced by the lunar cycle.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"8 1","pages":"33 - 40"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2004.9647731","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7