Artificial DNA: PNA & XNA最新文献_第5页

Potent and sustained cellular inhibition of miR-122 by lysine-derivatized peptide nucleic acids (PNA) and phosphorothioate locked nucleic acid (LNA)/2'-O-methyl (OMe) mixmer anti-miRs in the absence of transfection agents. 赖氨酸衍生肽核酸（PNA）和磷酸化锁定核酸(LNA)/2'- o -甲基（OMe）混合抗mir在没有转染剂的情况下有效和持续的细胞抑制miR-122。

Artificial DNA: PNA & XNA Pub Date : 2011-07-01 DOI: 10.4161/adna.17731

Adrian G Torres, Richard N Threlfall, Michael J Gait

{"title":"Potent and sustained cellular inhibition of miR-122 by lysine-derivatized peptide nucleic acids (PNA) and phosphorothioate locked nucleic acid (LNA)/2'-O-methyl (OMe) mixmer anti-miRs in the absence of transfection agents.","authors":"Adrian G Torres, Richard N Threlfall, Michael J Gait","doi":"10.4161/adna.17731","DOIUrl":"10.4161/adna.17731","url":null,"abstract":"Efficient cell delivery of antisense oligonucleotides (ONs) is a key issue for their potential therapeutic use. It has been shown recently that some ONs can be delivered into cells without the use of transfection agents (gymnosis), but this generally requires cell incubation over several days and high amounts of ONs (micromolar concentrations). Here we have targeted microRNA 122 (miR-122), a small non-coding RNA involved in regulation of lipid metabolism and in the replication of hepatitis C virus, with ONs of different chemistries (anti-miRs) by gymnotic delivery in cell culture. Using a sensitive dual-luciferase reporter assay, anti-miRs were screened for their ability to enter liver cells gymnotically and inhibit miR-122 activity. Efficient miR-122 inhibition was obtained with cationic PNAs and 2'-O-methyl (OMe) and Locked Nucleic Acids (LNA)/OMe mixmers containing either phosphodiester (PO) or phosphorothioate (PS) linkages at sub-micromolar concentrations when incubated with cells for just 4 hours. Furthermore, PNA and PS-containing anti-miRs were able to sustain miR-122 inhibitory effects for at least 4 days. LNA/OMe PS anti-miRs were the most potent anti-miR chemistry tested in this study, an ON chemistry that has been little exploited so far as anti-miR agents towards therapeutics.","PeriodicalId":8444,"journal":{"name":"Artificial DNA: PNA & XNA","volume":"2 3","pages":"71-8"},"PeriodicalIF":0.0,"publicationDate":"2011-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3324337/pdf/adna-2-71.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30603156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PNA HyBeacons for analysis of human mutations related to statin-induced myopathy. PNA HyBeacons用于分析与他汀类药物诱导的肌病相关的人类突变。

Artificial DNA: PNA & XNA Pub Date : 2011-07-01 DOI: 10.4161/adna.18179

Nittaya Gale, Petr Kocalka, Charlotte Mardle, Tom Brown

引用次数: 0

Peptide nucleic acid (PNA) cell penetrating peptide (CPP) conjugates as carriers for cellular delivery of antisense oligomers. 肽核酸(PNA)细胞穿透肽(CPP)偶联物作为反义寡聚物的细胞递送载体。

Artificial DNA: PNA & XNA Pub Date : 2011-07-01 DOI: 10.4161/adna.18739

Takehiko Shiraishi, Peter E Nielsen

{"title":"Peptide nucleic acid (PNA) cell penetrating peptide (CPP) conjugates as carriers for cellular delivery of antisense oligomers.","authors":"Takehiko Shiraishi, Peter E Nielsen","doi":"10.4161/adna.18739","DOIUrl":"https://doi.org/10.4161/adna.18739","url":null,"abstract":"We have explored the merits of a novel delivery strategy for the antisense oligomers based on cell penetrating peptide (CPP) conjugated to a carrier PNA with sequence complementary to part of the antisense oligomer. The effect of these carrier CPP-PNAs was evaluated by using antisense PNA targeting splicing correction of the mutated luciferase gene in the HeLa pLuc705 cell line, reporting cellular (nuclear) uptake of the antisense PNA via luciferase activity measurement. Carrier CPP-PNA constructs were studied in terms of construct modification (with octaarginine and/or decanoic acid) and carrier PNA length (to adjust binding affinity). In general, the carrier CPP-PNA constructs including the ones with decanoyl modification provided significant increase of the activity of unmodified antisense PNA as well as of antisense octaarginine-PNA conjugates. Antisense activity, and by inference cellular delivery, of unmodified antisense PNA was enhanced at least 20-fold at 6 μM upon the complexation with an equimolar amount of nonamer carrier decanoyl-CPP-PNA (Deca-cPNA1(9)-(D-Arg)8). The antisense activity of a CPP-PNA ((D-Arg)8-asPNA) (at 2 μM) was improved 6-fold and 8-fold by a heptamer carrier CPP-PNA (cPNA1(7)-(D-Arg)8) and hexamer carrier decanoyl-CPP-PNA (Deca-cPNA1(6)-(D-Arg)8), respectively, without showing significant additional cellular toxicity. Most interestingly, the activity reached the same level obtained by enhancement with endosomolytic chloroquine (CQ) treatment, suggesting that the carrier might facilitate endosomal escape. Furthermore, 50% downregulation of luciferase expression at 60 nM siRNA was obtained using this carrier CPP-PNA delivery strategy (with CQ co-treatment) for a single stranded antisense RNA targeting normal luciferase mRNA. These results indicated that CPP-PNA carriers may be used as effective cellular delivery vectors for different types of antisense oligomers and also allows use of combinations of (at least two) different CPP ligands.","PeriodicalId":8444,"journal":{"name":"Artificial DNA: PNA & XNA","volume":"2 3","pages":"90-9"},"PeriodicalIF":0.0,"publicationDate":"2011-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/adna.18739","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30603161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 47

RNA-DNA sequence differences spell genetic code ambiguities. RNA-DNA序列的差异导致了遗传密码的模糊性。

Artificial DNA: PNA & XNA Pub Date : 2011-07-01 DOI: 10.4161/adna.17086

Thomas Bentin, Michael L Nielsen

引用次数: 1

Artificial DNA structures. 人工DNA结构。

Artificial DNA: PNA & XNA Pub Date : 2011-04-01 DOI: 10.4161/adna.2.2.17085

Peter E Nielsen

引用次数: 0

Noncovalent binding and fluorogenic response of cyanine dyes to DNA homoquadruplex and PNA-DNA heteroquadruplex structures. 花青素染料对DNA同型四重体和PNA-DNA异型四重体结构的非共价结合和荧光反应。

Artificial DNA: PNA & XNA Pub Date : 2011-04-01 DOI: 10.4161/adna.2.2.16339

Halimatu S Mohammed, Junriz O Delos Santos, Bruce A Armitage

引用次数: 17

A ribozyme transcribed by a ribozyme. 核酶由核酶转录的核酶

Artificial DNA: PNA & XNA Pub Date : 2011-04-01 DOI: 10.4161/adna.2.2.16852

Thomas Bentin

引用次数: 1

Pyrrolidinyl peptide nucleic acid with α/β-peptide backbone: A conformationally constrained PNA with unusual hybridization properties. 具有α/β-肽骨架的吡咯烷基肽核酸:一种具有不同寻常杂交特性的构象约束的PNA。

Artificial DNA: PNA & XNA Pub Date : 2011-04-01 DOI: 10.4161/adna.2.2.16340

Chotima Vilaivan, Choladda Srisuwannaket, Cheeraporn Ananthanawat, Chaturong Suparpprom, Junji Kawakami, Yoshie Yamaguchi, Yuko Tanaka, Tirayut Vilaivan

{"title":"Pyrrolidinyl peptide nucleic acid with α/β-peptide backbone: A conformationally constrained PNA with unusual hybridization properties.","authors":"Chotima Vilaivan, Choladda Srisuwannaket, Cheeraporn Ananthanawat, Chaturong Suparpprom, Junji Kawakami, Yoshie Yamaguchi, Yuko Tanaka, Tirayut Vilaivan","doi":"10.4161/adna.2.2.16340","DOIUrl":"https://doi.org/10.4161/adna.2.2.16340","url":null,"abstract":"We describe herein a new conformationally constrained analog of PNA carrying an alternating α/β amino acid backbone consisting of (2'R,4'R)-nucleobase-subtituted proline and (1S,2S)-2-aminocyclopentanecarboxylic acid (acpcPNA). The acpcPNA has been synthesized and evaluated for DNA, RNA and self-pairing properties by thermal denaturation experiments. It can form antiparallel hybrids with complementary DNA with high affinity and sequence specificity. Unlike other PNA systems, the thermal stability of acpcPNA·DNA hybrid is largely independent of G+C contents, and is generally higher than that of acpcPNA·RNA hybrid with the same sequence. Thermodynamic parameters analysis suggest that the A·T base pairs in the acpcPNA·DNA hybrids are enthalpically stabilized over G·C pairs. The acpcPNA also shows a hitherto unreported behavior, namely the inability to form self-pairing hybrids. These unusual properties should make the new acpcPNA a potentially useful candidate for various applications including microarray probes and antigene agents.","PeriodicalId":8444,"journal":{"name":"Artificial DNA: PNA & XNA","volume":"2 2","pages":"50-59"},"PeriodicalIF":0.0,"publicationDate":"2011-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/adna.2.2.16340","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29991668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 35

Sensitive detection of nucleic acids by PNA hybridization directed co-localization of fluorescent beads. 利用PNA杂交定向荧光珠共定位的核酸敏感检测方法。

Artificial DNA: PNA & XNA Pub Date : 2011-04-01 DOI: 10.4161/adna.2.2.16562

Takehiko Shiraishi, Stijn Deborggraeve, Philippe Büscher, Peter E Nielsen

{"title":"Sensitive detection of nucleic acids by PNA hybridization directed co-localization of fluorescent beads.","authors":"Takehiko Shiraishi, Stijn Deborggraeve, Philippe Büscher, Peter E Nielsen","doi":"10.4161/adna.2.2.16562","DOIUrl":"https://doi.org/10.4161/adna.2.2.16562","url":null,"abstract":"We have designed a pair of biotinylated peptide nucleic acid (PNA) probes targeting two sequences in 18S rRNA (from the parasite Trypanosoma brucei) at a distance of 191 nt (corresponding to maximum distance of ca. 60 nm) from each other. The PNA probes were individually bound to (strept)avidin-coated fluorescent beads, differing in size and color [green beads (1 µm) and red beads (5.9 µm)], thereby allowing distinct detection of each PNA probe by conventional fluorescence microscopy. These two PNA beads showed easily detectable co-localization when simultaneously hybridizing to a target nucleic acid. The assay detected the parasite 18S rRNA down to 1.6 fmol while there was no such co-localization visible with human 18S rRNA not containing the PNA targets. Furthermore, the assay showed positive detection with 1.6 ng of total RNA (corresponding to RNA from ca. 300 parasites). Upon further optimization this method may provide a new tool for a diagnosis of Human African Trypanosomiasis (HAT) and it may more generally have applications within diagnostics for (neglected) infectious diseases.","PeriodicalId":8444,"journal":{"name":"Artificial DNA: PNA & XNA","volume":"2 2","pages":"60-66"},"PeriodicalIF":0.0,"publicationDate":"2011-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/adna.2.2.16562","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29991669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

A novel pseudo-complementary PNA G-C base pair. 一种新型伪互补PNA G-C碱基对。

Artificial DNA: PNA & XNA Pub Date : 2011-01-01 DOI: 10.4161/adna.2.1.15554

Anne G Olsen, Otto Dahl, Asger B Petersen, John Nielsen, Peter E Nielsen

引用次数: 13