Nittaya Gale, Petr Kocalka, Charlotte Mardle, Tom Brown
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PNA HyBeacons for analysis of human mutations related to statin-induced myopathy.
Aminoalkyl and alkyne-tagged PNA HyBeacons have been synthesized, labeled with fluorescein via conventional amide bond or triazole formation (click chemistry) and used to detect single nucleotide polymorphisms (SNPs) implicated in statin-induced myopathy. The PNA HyBeacons gave much better mismatch/mutant discrimination than conventional DNA HyBeacons but smaller fluorescence changes on melting.
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