Artificial DNA: PNA & XNA最新文献_第3页

Artificial DNA: PNA & XNA Volume 4, Issue 1 Table of Contents 人工DNA: PNA & XNA第4卷，第1期目录

Artificial DNA: PNA & XNA Pub Date : 2013-01-01 DOI: 10.4161/adna.24467

引用次数: 0

Fluorescent triplex-forming DNA oligonucleotides labeled with a thiazole orange dimer unit. 用噻唑橙二聚体标记的荧光三聚体形成的DNA寡核苷酸。

Artificial DNA: PNA & XNA Pub Date : 2013-01-01 DOI: 10.4161/adna.24102

Shuji Ikeda, Hiroyuki Yanagisawa, Mizue Yuki, Akimitsu Okamoto

引用次数: 7

Di-heterometalation of thiol-functionalized peptide nucleic acids. 巯基功能化肽核酸的二氢化。

Artificial DNA: PNA & XNA Pub Date : 2013-01-01 DOI: 10.4161/adna.24019

Tanmaya Joshi, Malay Patra, Leone Spiccia, Gilles Gasser

{"title":"Di-heterometalation of thiol-functionalized peptide nucleic acids.","authors":"Tanmaya Joshi, Malay Patra, Leone Spiccia, Gilles Gasser","doi":"10.4161/adna.24019","DOIUrl":"10.4161/adna.24019","url":null,"abstract":"As a proof-of-principle, two hetero-bimetallic PNA oligomers containing a ruthenium(II) polypyridyl and a cyclopentadienyl manganese tricarbonyl complex have been prepared by serial combination of solid-phase peptide coupling and in-solution thiol chemistry. Solid-phase N-terminus attachment of Ru(II)-polypyridyl carboxylic acid derivative, C1, onto the thiol-functionalized PNA backbone (H-a-a-g-t-c-t-g-c-linker-cys-NH 2) has been performed by standard peptide coupling method. As two parallel approaches, the strong affinity of thiols for maleimide and haloacetyl group has been exploited for subsequent post-SPPS addition of cymantrene-based organometallic cores, C2 and C3. Michael-like addition and thioether ligation of thiol functionalized PNA1 (H-gly-a-a-g-t-c-t-g-c-linker-cys-NH 2) and PNA2 (C1-a-a-g-t-c-t-g-c-linker-cys-NH 2) to cymantrene maleimide and chloroacetyl derivatives, C2 and C3, respectively, has been performed. The synthesized ruthenium(II)-cymantrenyl PNA oligomers have been characterized by mass spectrometry (ESI-MS) and IR spectroscopy. The distinct Mn-CO vibrational IR stretches, between 1,924-2,074 cm (-1) , have been used as markers to confirm the presence of cymantrenyl units in the PNA sequences and the purity of the HPLC-purified PNA thioethers assessed using LC-MS.","PeriodicalId":8444,"journal":{"name":"Artificial DNA: PNA & XNA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654725/pdf/adna-4-11.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31250031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nucleic acid sensing by an orthogonal reporter system based on homo-DNA. 基于同源dna的正交报告系统的核酸传感。

Artificial DNA: PNA & XNA Pub Date : 2013-01-01 DOI: 10.4161/adna.24227

Matthias Stoop, Camille Désiron, Christian J Leumann

引用次数: 4

DNA display of PNA-tagged ligands: a versatile strategy to screen libraries and control geometry of multidentate ligands. rna标记配体的DNA显示:一种多齿配体筛选文库和控制几何形状的通用策略。

Artificial DNA: PNA & XNA Pub Date : 2012-07-01 DOI: 10.4161/adna.21108

Nicolas Winssinger

引用次数: 8

To DNA, all information is equal. 对DNA来说，所有的信息都是平等的。

Artificial DNA: PNA & XNA Pub Date : 2012-07-01 DOI: 10.4161/adna.22671

Lau Sennels, Thomas Bentin

引用次数: 3

Templating effect in DNA proximity ligation enables use of non-bioorthogonal chemistry in biological fluids. DNA接近结扎中的模板效应使得在生物流体中使用非生物正交化学成为可能。

Artificial DNA: PNA & XNA Pub Date : 2012-07-01 DOI: 10.4161/adna.23842

Nicholas G Spiropulos, Jennifer M Heemstra

{"title":"Templating effect in DNA proximity ligation enables use of non-bioorthogonal chemistry in biological fluids.","authors":"Nicholas G Spiropulos, Jennifer M Heemstra","doi":"10.4161/adna.23842","DOIUrl":"https://doi.org/10.4161/adna.23842","url":null,"abstract":"Here we describe the first example of selective reductive amination in biological fluids using split aptamer proximity ligation (StAPL). Utilizing the cocaine split aptamer, we demonstrate small-molecule-dependent ligation that is dose-dependent over a wide range of target concentrations in buffer, human blood serum and artificial urine medium. We explore the substrate binding preferences of the split aptamer and find that the cinchona alkaloids quinine and quinidine bind to the aptamer with higher affinity than cocaine. This increased affinity leads to improved detection limits for these small-molecule targets. We also demonstrate that linker length and hydrophobicity impact the efficiency of split aptamer ligation. The ability to carry out selective chemical transformations using non-bioorthogonal chemistry in media where competing reactive groups are present highlights the power of the increased effective molarity provided by DNA assembly. Obviating the need for bioorthogonal chemistry would dramatically expand the repertoire of chemical transformations available for use in templated reactions such as proximity ligation assays, in turn enabling the development of novel methods for biomolecule detection.","PeriodicalId":8444,"journal":{"name":"Artificial DNA: PNA & XNA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/adna.23842","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31297490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Peptide nucleic acids in materials science. 材料科学中的肽核酸。

Artificial DNA: PNA & XNA Pub Date : 2012-07-01 DOI: 10.4161/adna.21941

Davide Bonifazi, Laure-Elie Carloni, Valentina Corvaglia, Arnaud Delforge

引用次数: 17

Peptide nucleic acids tagged with four lysine residues for amperometric genosensors. 用四个赖氨酸残基标记的肽核酸用于安培基因传感器。

Artificial DNA: PNA & XNA Pub Date : 2012-04-01 DOI: 10.4161/adna.20777

Chiara Zanardi, Fabio Terzi, Renato Seeber, Clara Baldoli, Emanuela Licandro, Stefano Maiorana

{"title":"Peptide nucleic acids tagged with four lysine residues for amperometric genosensors.","authors":"Chiara Zanardi, Fabio Terzi, Renato Seeber, Clara Baldoli, Emanuela Licandro, Stefano Maiorana","doi":"10.4161/adna.20777","DOIUrl":"https://doi.org/10.4161/adna.20777","url":null,"abstract":"A homothymine PNA decamer bearing four lysine residues has been synthesized as a probe for the development of amperometric sensors. On one hand, the four amino groups introduced make this derivative nine times more soluble than the corresponding homothymine PNA decamer and, on the other hand, allow the stable anchoring of this molecule on Au nanostructured surface through the terminal -NH 2 moieties. In particular, XPS and electrochemical investigations performed with hexylamine, as a model molecule, indicate that the stable deposition of primary amine derivatives on such a nanostructured surface is possible and involves the free electron doublet on the nitrogen atom. This finding indicates that this PNA derivative is suitable to act as the probe molecule for the development of amperometric sensors. Thanks to the molecular probe chosen and to the use of a nanostructured surface as the substrate for the sensor assembly, the device proposed makes possible the selective recognition of the target oligonucleotide sequence with very high sensitivity.","PeriodicalId":8444,"journal":{"name":"Artificial DNA: PNA & XNA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/adna.20777","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30745286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Helix control in polymers: case of peptide nucleic acids (PNAs). 聚合物中的螺旋控制：肽核酸 (PNA) 案例。

Artificial DNA: PNA & XNA Pub Date : 2012-04-01 DOI: 10.4161/adna.20572

Filbert Totsingan, Vipul Jain, Mark M Green

{"title":"Helix control in polymers: case of peptide nucleic acids (PNAs).","authors":"Filbert Totsingan, Vipul Jain, Mark M Green","doi":"10.4161/adna.20572","DOIUrl":"10.4161/adna.20572","url":null,"abstract":"The helix is a critical conformation exhibited by biological macromolecules and plays a key role in fundamental biological processes. Biological helical polymers exist in a single helical sense arising from the chiral effect of their primary units-for example, DNA and proteins adopt predominantly a right-handed helix conformation in response to the asymmetric conformational propensity of D-sugars and L-amino acids, respectively. In using these homochiral systems, nature blocks our observations of some fascinating aspects of the cooperativity in helical systems, although when useful for a specific purpose, \"wrong\" enantiomers may be incorporated in specific places. In synthetic helical systems, on the contrary, incorporation of non-racemic chirality is an additional burden, and the findings discussed in this review show that this burden may be considerably alleviated by taking advantage of the amplification of chirality, in which small chiral influences lead to large consequences. Peptide nucleic acid (PNA), which is a non-chiral synthetic DNA mimic, shows a cooperative response to a small chiral effect induced by a chiral amino acid, which is limited, however, due to the highly flexible nature of this oligomeric chimera. The lack of internal stereochemical bias is an important factor which makes PNA an ideal system to understand some cooperative features that are not directly accessible from DNA.","PeriodicalId":8444,"journal":{"name":"Artificial DNA: PNA & XNA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/72/95/adna-3-31.PMC3429529.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30745227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0