Peptide nucleic acids tagged with four lysine residues for amperometric genosensors.

Chiara Zanardi, Fabio Terzi, Renato Seeber, Clara Baldoli, Emanuela Licandro, Stefano Maiorana
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引用次数: 8

Abstract

A homothymine PNA decamer bearing four lysine residues has been synthesized as a probe for the development of amperometric sensors. On one hand, the four amino groups introduced make this derivative nine times more soluble than the corresponding homothymine PNA decamer and, on the other hand, allow the stable anchoring of this molecule on Au nanostructured surface through the terminal -NH 2 moieties. In particular, XPS and electrochemical investigations performed with hexylamine, as a model molecule, indicate that the stable deposition of primary amine derivatives on such a nanostructured surface is possible and involves the free electron doublet on the nitrogen atom. This finding indicates that this PNA derivative is suitable to act as the probe molecule for the development of amperometric sensors. Thanks to the molecular probe chosen and to the use of a nanostructured surface as the substrate for the sensor assembly, the device proposed makes possible the selective recognition of the target oligonucleotide sequence with very high sensitivity.

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用四个赖氨酸残基标记的肽核酸用于安培基因传感器。
合成了含有四个赖氨酸残基的同胸腺嘧啶PNA十聚体,作为开发安培传感器的探针。一方面,引入的四个氨基使该衍生物的可溶性比相应的同型胸腺嘧啶PNA十聚体高9倍,另一方面,通过末端- nh2部分,该分子可以稳定地锚定在金纳米结构表面上。特别是,以己胺为模型分子进行的XPS和电化学研究表明,伯胺衍生物在这种纳米结构表面上的稳定沉积是可能的,并且涉及氮原子上的自由电子对偶态。这一发现表明该PNA衍生物适合作为探针分子用于开发安培传感器。由于所选择的分子探针和使用纳米结构表面作为传感器组件的衬底,所提出的装置可以以非常高的灵敏度选择性识别目标寡核苷酸序列。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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