The journal of microcolumn separations : JMS最新文献_第10页

Application of capillary electrophoresis for trace ion analysis in rain water† 毛细管电泳在雨水中痕量离子分析中的应用

The journal of microcolumn separations : JMS Pub Date : 2000-06-16 DOI: 10.1002/1520-667X(2000)12:6<337::AID-MCS1>3.0.CO;2-O

Y. S. Fung, H. S. Tung

{"title":"Application of capillary electrophoresis for trace ion analysis in rain water†","authors":"Y. S. Fung, H. S. Tung","doi":"10.1002/1520-667X(2000)12:6<337::AID-MCS1>3.0.CO;2-O","DOIUrl":"10.1002/1520-667X(2000)12:6<337::AID-MCS1>3.0.CO;2-O","url":null,"abstract":"Solving the problem due to transition metals' comigration during electrokinetic injection, the transient isotachophoresis (ITP) procedure was developed to preconcentrate and focus transition metals during electrokinetic injection. It was found to provide a suitable method for trace metals' preconcentration from rainwater at sub-μg/L level from buffer system consisting of 30 mM hydroxylamine hydrochloride, 0.1 mM 1,10-phenanthroline, 1% methanol, 15 mM ammonium chloride, and 0.08 M urea at pH=3.7. Trace metals as Cd2+, Zn2+, Cu2+, Co2+, and Fe2+ (total Fe determined as Fe2+) are determined with working range from 0.5–80 μg/L, repeatability less than 7%, and detection limits from 0.1 to 0.5 μg/L under optimized operation conditions as follows: Injection at 3 kV for 40 s prior to CE run at +15 kV in buffer system developed and with 0.4 mM tetrabutyl ammonium bromide added to rain sample as terminating ion. Leading and terminating ion effect, buffer additive, and operation conditions on performance of ITP procedure were investigated. Procedure developed was applied for trace metals' determination in rain water collected in Hong Kong. It provides fast, efficient, and economic method determining transition metals in rain sample down to sub-μg/L level, reducing sample pretreatment time and eliminating off-column preconcentration steps often leading to contamination problems for transition metals determination at μg/L level. © 2000 John Wiley & Sons, Inc. J Micro Sep 12: 337–344, 2000","PeriodicalId":83120,"journal":{"name":"The journal of microcolumn separations : JMS","volume":"12 6","pages":"337-344"},"PeriodicalIF":0.0,"publicationDate":"2000-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/1520-667X(2000)12:6<337::AID-MCS1>3.0.CO;2-O","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50651224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Microcolumn abstracts 微柱凝集抽象

The journal of microcolumn separations : JMS Pub Date : 2000-06-16 DOI: 10.1002/1520-667X(2000)12:6<378::AID-MCS7>3.0.CO;2-%23

Douglas E. Raynie

引用次数: 0

Quantitative chiral analysis of carbinoxamine, doxylamine, and orphenadrine by capillary zone electrophoresis† 毛细管区带电泳定量手性分析卡比诺胺，多西胺，和奥菲那定

The journal of microcolumn separations : JMS Pub Date : 2000-06-16 DOI: 10.1002/1520-667X(2000)12:6<366::AID-MCS5>3.0.CO;2-C

Yi-Fen Tang, Hsin-Lung Wu, Shou-Mei Wu, Su-Hwei Chen, Hwang-Shang Kou

{"title":"Quantitative chiral analysis of carbinoxamine, doxylamine, and orphenadrine by capillary zone electrophoresis†","authors":"Yi-Fen Tang, Hsin-Lung Wu, Shou-Mei Wu, Su-Hwei Chen, Hwang-Shang Kou","doi":"10.1002/1520-667X(2000)12:6<366::AID-MCS5>3.0.CO;2-C","DOIUrl":"10.1002/1520-667X(2000)12:6<366::AID-MCS5>3.0.CO;2-C","url":null,"abstract":"A simple capillary zone electrophoresis method is described for the simultaneous separation and quantitation of chiral carbinoxamine maleate, doxylamine succinate, and orphenadrine citrate using achiral diphenhydramine⋅HCl as an internal standard. The chiral analysis of these drugs was performed in a Tris buffer (100 mM; pH 4.60) with sulfated β-cyclodextrin (15 mg/mL) as a chiral selector. Several parameters affecting the separation were studied, including the pH of the buffer and the concentrations of buffer and chiral selector. Quantitation of the individual enantiomer (prepared from the related racemate) is attainable at 25–125 μM for carbinoxamine maleate, doxylamine succinate, or orphenadrine citrate. The migration order of the separated enantiomers is compared to that of a structurally related dexchlorpheniramine, an S-enantiomer of chlorpheniramine. © 2000 John Wiley & Sons, Inc. J Micro Sep 12: 366–370, 2000","PeriodicalId":83120,"journal":{"name":"The journal of microcolumn separations : JMS","volume":"12 6","pages":"366-370"},"PeriodicalIF":0.0,"publicationDate":"2000-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/1520-667X(2000)12:6<366::AID-MCS5>3.0.CO;2-C","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50650825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Fast gas chromatography: The effect of fast temperature programming† 快速气相色谱法:快速温度编程的影响

The journal of microcolumn separations : JMS Pub Date : 2000-06-16 DOI: 10.1002/1520-667X(2000)12:6<351::AID-MCS3>3.0.CO;2-Y

H. M. McNair, G. L. Reed

{"title":"Fast gas chromatography: The effect of fast temperature programming†","authors":"H. M. McNair, G. L. Reed","doi":"10.1002/1520-667X(2000)12:6<351::AID-MCS3>3.0.CO;2-Y","DOIUrl":"10.1002/1520-667X(2000)12:6<351::AID-MCS3>3.0.CO;2-Y","url":null,"abstract":"Fast gas chromatography (GC) has been around ever since the introduction of capillary GC columns. M. Golay's patent application for capillary GC (Patent 2,920,478, January 12, 1960) showed five peaks in 15 s in 1957. Desty et al. (Desty, D. H.; Goldup, A.; Swanton, W. T. In Gas Chromatography; Brenner, N.; Caller, J. E.; Weiss, M. D., Eds.; Academic: New York, 1962; p. 105), LeClercq et al. (LeClercq, P. A.; Scherpenzeel, G. J.; Vermeer, E. A. A.; Cramers, C. A. J Chromatogr 1982, 241, 61–71), and Cramers et al. (Schutjes, C.; Vermeer, E.; Rijks, J.; Cramers, C. J Chromatogr 1982, 253, 1–16), among others, followed up this early work on fast GC. The work in this paper is a continuation of fast GC, primarily using fast oven temperature programming. This work is restricted to normal pressures, routinely used on Hewlett Packard 5890 and 6890 model gas chromatographs. The first step in doing fast GC is to reduce the column length, since the retention time is directly proportional to the length, only true for isothermal runs. Columns of 1 to 6 m are used and since this step sometimes sacrifices resolution, 100 μm inner diameter columns with thin films, 0.1 to 0.2 μm, are used with small sample sizes (split ratios of 500:1 to 2000:1). © 2000 John Wiley & Sons, Inc. J Micro Sep 12: 351–355, 2000","PeriodicalId":83120,"journal":{"name":"The journal of microcolumn separations : JMS","volume":"12 6","pages":"351-355"},"PeriodicalIF":0.0,"publicationDate":"2000-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/1520-667X(2000)12:6<351::AID-MCS3>3.0.CO;2-Y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50651271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Features of metal capillary columns† 金属毛细管柱的特点

The journal of microcolumn separations : JMS Pub Date : 2000-06-16 DOI: 10.1002/1520-667X(2000)12:6<345::AID-MCS2>3.0.CO;2-Q

C. Watanabe, M. Morikawa, Y. Takayama

引用次数: 6

Multivariate optimization in micellar electrokinetic capillary chromatography 胶束电动毛细管色谱的多元优化

The journal of microcolumn separations : JMS Pub Date : 2000-06-16 DOI: 10.1002/1520-667X(2000)12:6<356::AID-MCS4>3.0.CO;2-G

Ruijiang Lee, Zhen Liu, Hanfa Zou, Jianyi Ni, Yukui Zhang

{"title":"Multivariate optimization in micellar electrokinetic capillary chromatography","authors":"Ruijiang Lee, Zhen Liu, Hanfa Zou, Jianyi Ni, Yukui Zhang","doi":"10.1002/1520-667X(2000)12:6<356::AID-MCS4>3.0.CO;2-G","DOIUrl":"10.1002/1520-667X(2000)12:6<356::AID-MCS4>3.0.CO;2-G","url":null,"abstract":"The optimization of the organic modifier concentration in micellar electrokinetic capillary chromatography (MECC) has been achieved by a uniform design and iterative optimization method, which has been developed for the optimization of composition of the mobile phase in high performance liquid chromatography. According to the proposed method, the uniform design technique has been applied to design the starting experiments, which can reduce the number of experiments compared with traditional simultaneous methods, such as the orthano design. The hierarchical chromatographic response function has been modified to evaluate the separation quality of a chromatogram in MECC. An iterative procedure has been adopted to search the optimal concentration of organic modifiers for improving the accuracy of retention predicted and the quality of the chromatogram. Validity of the optimization method has been proved by the separation of 31 aromatic compounds in MECC. © 2000 John Wiley & Sons, Inc. J Micro Sep 12: 356–365, 2000","PeriodicalId":83120,"journal":{"name":"The journal of microcolumn separations : JMS","volume":"12 6","pages":"356-365"},"PeriodicalIF":0.0,"publicationDate":"2000-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/1520-667X(2000)12:6<356::AID-MCS4>3.0.CO;2-G","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50650816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Evaluation of solid-phase microextraction in combination with gas chromatography (SPME-GC) as a tool for quantitative bioanalysis 固相微萃取和气相色谱(SPME-GC)联用作为定量生物分析工具的评价

The journal of microcolumn separations : JMS Pub Date : 2000-04-19 DOI: 10.1002/(SICI)1520-667X(2000)12:5<308::AID-MCS5>3.0.CO;2-F

Mohamed Abdel-Rehim, Margareta Bielenstein, Torbjörn Arvidsson

{"title":"Evaluation of solid-phase microextraction in combination with gas chromatography (SPME-GC) as a tool for quantitative bioanalysis","authors":"Mohamed Abdel-Rehim, Margareta Bielenstein, Torbjörn Arvidsson","doi":"10.1002/(SICI)1520-667X(2000)12:5<308::AID-MCS5>3.0.CO;2-F","DOIUrl":"10.1002/(SICI)1520-667X(2000)12:5<308::AID-MCS5>3.0.CO;2-F","url":null,"abstract":"Solid-phase microextraction in combination with capillary gas chromatography and a nitrogen–phosphorus detector as a bioanalysis tool was investigated. Lidocaine and three of its metabolites were used as model compounds, and human plasma and urine samples were used in this evaluation. Carbowax–divinylbenzene, polyacrylate, and polydimethylsiloxane fibers were tested. Absorption times were studied for all analytes separately. Carbowax–divinylbenzene fiber gave highest recovery in plasma samples compared to other fibers. Effects of temperature, addition of salt and agitation of the sample were studied. Recovery from plasma was improved by 2–4 times at pH 9 compared to pH 3. This is due to analytes not charged at high pH. Recovery from water was 2–4 times higher than from plasma using Carbowax–divinylbenzene coated fiber. This is due to protein binding of analytes in plasma. Chromatographic selectivity was high and all metabolites were well separated. Calibration curves were linear for all metabolites in human plasma and urine in the range 0.035–7.7 μM for lidocaine and 2,6-xylidine while 0.1–3.5 μM for glycinexylidide (GX) and monoethylglycinexylidide (MEGX). Precision, measures as relative standard deviation, was less than 15% and accuracy was in the range 80–115%. Limits of quantitation using plasma were 0.035 μM (8 ng/mL), 0.035 μM (4 ng/mL), 0.100 μM (18 ng/mL), and 0.100 μM (21 ng/mL) for lidocaine, 2,6-xylidine, GX, and MEGX, respectively. © 2000 John Wiley & Sons, Inc. J Micro Sep 12: 308–315, 2000","PeriodicalId":83120,"journal":{"name":"The journal of microcolumn separations : JMS","volume":"12 5","pages":"308-315"},"PeriodicalIF":0.0,"publicationDate":"2000-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1520-667X(2000)12:5<308::AID-MCS5>3.0.CO;2-F","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50646355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 35

On-line solid-phase extraction in microcolumn- liquid chromatography coupled to UV or MS detection: Application to the analysis of cyanobacterial toxins 微柱液相色谱联用紫外或质谱在线固相萃取:在蓝藻毒素分析中的应用

The journal of microcolumn separations : JMS Pub Date : 2000-04-19 DOI: 10.1002/(SICI)1520-667X(2000)12:5<323::AID-MCS7>3.0.CO;2-M

C. Rivasseau, G. Vanhoenacker, P. Sandra, M.-C. Hennion

{"title":"On-line solid-phase extraction in microcolumn- liquid chromatography coupled to UV or MS detection: Application to the analysis of cyanobacterial toxins","authors":"C. Rivasseau, G. Vanhoenacker, P. Sandra, M.-C. Hennion","doi":"10.1002/(SICI)1520-667X(2000)12:5<323::AID-MCS7>3.0.CO;2-M","DOIUrl":"10.1002/(SICI)1520-667X(2000)12:5<323::AID-MCS7>3.0.CO;2-M","url":null,"abstract":"An on-line technique coupling preconcentration via a microprecolumn with microcolumn-liquid chromatography (micro-LC) on a 1-mm inner diameter (i.d.) analytical column was investigated. The system was found to be very simple, fast, and efficient for the trace-level determination of some natural hepatotoxins synthesized by Cyanobacteria, namely, microcystins, in environmental water. Particular attention was paid to band broadening, and a good quality coupling was obtained. A cleanup was introduced in the solid-phase extraction (SPE) sequence to eliminate part of the interferents co- extracted during the sample percolation. The technique was reproducible, with relative standard deviations ranging from 3 to 8%, linear in the range 0.05–50 μg/L. Microcystins could be detected below the 0.04 μg/L level in drinking and surface water using only 5-mL sample volumes, with UV detection. The very small sample volume, resulting from lower detection limits, in absolute amounts, obtained in micro-LC represents a definite advantage over on-line classical chromatography. The suitability of on-line micro-SPE–micro-LC–MS was also evaluated. Application to the determination of microcystins in environmental and drinking water samples are presented. © 2000 John Wiley & Sons, Inc. J Micro Sep 12: 323–332, 2000","PeriodicalId":83120,"journal":{"name":"The journal of microcolumn separations : JMS","volume":"12 5","pages":"323-332"},"PeriodicalIF":0.0,"publicationDate":"2000-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1520-667X(2000)12:5<323::AID-MCS7>3.0.CO;2-M","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50646410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Pulsed voltage induced injection for capillary liquid chromatography† 脉冲电压诱导注射毛细管液相色谱法†

The journal of microcolumn separations : JMS Pub Date : 2000-04-19 DOI: 10.1002/(SICI)1520-667X(2000)12:5<285::AID-MCS2>3.0.CO;2-S

Shinya Kitagawa, Takao Tsuda

{"title":"Pulsed voltage induced injection for capillary liquid chromatography†","authors":"Shinya Kitagawa, Takao Tsuda","doi":"10.1002/(SICI)1520-667X(2000)12:5<285::AID-MCS2>3.0.CO;2-S","DOIUrl":"10.1002/(SICI)1520-667X(2000)12:5<285::AID-MCS2>3.0.CO;2-S","url":null,"abstract":"A new injection method for capillary liquid chromatography named “pulsed voltage induced injection (PVII)” is proposed. In this injection method, effluent is used as a sample solution. The PVII is based on the variation of the capacity factor induced by an application of voltage. The application of pulsed voltage along a column generates a specific zone around the column inlet where the matrix of solution differs from the sample solution. The basic behavior of the pulsed voltage induced injection has been studied by using a cation exchange column (inner diameter 150 μm, packed length 13.6 mm) and the sample solution of 5 mM CuCl2 aqueous solution containing NaCl and KCl. Peak heights of Na+ and K+ increased with the increment of the magnitude of the applied voltage. A linear correlation between the concentration of K+ in the sample solution and the peak height of K+ was obtained by using an internal standard method. © 2000 John Wiley & Sons, Inc. J Micro Sep 12: 285–291, 2000","PeriodicalId":83120,"journal":{"name":"The journal of microcolumn separations : JMS","volume":"12 5","pages":"285-291"},"PeriodicalIF":0.0,"publicationDate":"2000-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1520-667X(2000)12:5<285::AID-MCS2>3.0.CO;2-S","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50646716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Activity of different proteases in a complex mixture and in vitro study of their reciprocal interferences by micellar electrokinetic chromatography 不同蛋白酶在复杂混合物中的活性及其相互干扰的体外胶束电动色谱研究

The journal of microcolumn separations : JMS Pub Date : 2000-04-19 DOI: 10.1002/(SICI)1520-667X(2000)12:5<302::AID-MCS4>3.0.CO;2-%23

S. Viglio, A. Lupi, M. Luisetti, G. Zanaboni, G. Cetta, P. Iadarola

引用次数: 0