Pulsed voltage induced injection for capillary liquid chromatography†

A new injection method for capillary liquid chromatography named “pulsed voltage induced injection (PVII)” is proposed. In this injection method, effluent is used as a sample solution. The PVII is based on the variation of the capacity factor induced by an application of voltage. The application of pulsed voltage along a column generates a specific zone around the column inlet where the matrix of solution differs from the sample solution. The basic behavior of the pulsed voltage induced injection has been studied by using a cation exchange column (inner diameter 150 μm, packed length 13.6 mm) and the sample solution of 5 mM CuCl₂ aqueous solution containing NaCl and KCl. Peak heights of Na⁺ and K⁺ increased with the increment of the magnitude of the applied voltage. A linear correlation between the concentration of K⁺ in the sample solution and the peak height of K⁺ was obtained by using an internal standard method. © 2000 John Wiley & Sons, Inc. J Micro Sep 12: 285–291, 2000