Archives of histology and cytology最新文献

{"title":"Nano-scale analyses of the chromatin decompaction induced by histone acetylation.","authors":"Kohji Hizume,&nbsp;Sumiko Araki,&nbsp;Kosuke Hata,&nbsp;Eloise Prieto,&nbsp;Tapas K Kundu,&nbsp;Kenichi Yoshikawa,&nbsp;Kunio Takeyasu","doi":"10.1679/aohc.73.149","DOIUrl":"https://doi.org/10.1679/aohc.73.149","url":null,"abstract":"<p><p>The acetylation of histone tails is a key factor in the maintenance of chromatin dynamics and cellular homeostasis. The hallmark of active chromatin is the hyper-acetylation of histones, which appears to result in a more open chromatin structure. Although short nucleosomal arrays have been studied, the structural dynamics of relatively long acetylated chromatin remain unclear. We have analyzed in detail the structure of long hyper-acetylated chromatin fibers using atomic force microscopy (AFM). Hyper-acetylated chromatin fibers isolated from nuclei that had been treated with Trichostatin A (TSA), an inhibitor of histone deacetylase, were found to be thinner than those from untreated nuclei. The acetylated chromatin fibers were more easily spread out of nuclei by high-salt treatment, implying that hyper-acetylation facilitates the release of chromatin fibers from compact heterochromatin regions. Chromatin fibers reconstituted in vitro from core histones and linker histone H1 became thinner upon acetylation. AFM imaging indicated that the gyration radius of the nucleosomal fiber increased after acetylation and that the hyper-acetylated nucleosomes did not aggregate at high salt concentrations, in contrast to the behavior of non-acetylated nucleosomal arrays, suggesting that acetylation increases long-range repulsions between nucleosomes. Based on these data, we considered a simple coarse grained model, which underlines the effect of remaining electric charges inside the chromatin fiber.</p>","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":"73 3","pages":"149-63"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.73.149","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30606232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PKH26 is an excellent retrograde and anterograde fluorescent tracer characterized by a small injection site and strong fluorescence emission.","authors":"Koji Kawaguchi,&nbsp;Yu Katsuyama,&nbsp;Satoshi Kikkawa,&nbsp;Tomiyoshi Setsu,&nbsp;Toshio Terashima","doi":"10.1679/aohc.73.65","DOIUrl":"https://doi.org/10.1679/aohc.73.65","url":null,"abstract":"<p><p>The fluorescent dye PKH26, which binds mainly to the cell membrane, has long stability that enables the tracing of PKH26-labeled transplanted cells in host tissue. In the present study, we examined whether this fluorescent dye works as a retrograde or anterograde tracer to label neural networks within the central nervous system of adult and postnatal day 3 (P3) mice. A small injection of the dye into the medullospinal junction resulted in the retrograde labeling of corticospinal tract (CST) neurons in layer V of the sensory-motor cortex both in the adult mice and pups. Injection of the dye into the motor cortex of the P3 pups resulted in the anterograde labeling of CST fibers at a single fiber resolution level, although a similar injection of the dye into the motor cortex of the adult mice failed to stain CST fibers anterogradely. These results suggest that, while PKH26 works as a retrograde or anterograde tracer, anterograde labeling of the adult tracts can not be expected.</p>","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":"73 2","pages":"65-72"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.73.65","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29877859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microvascular anatomy of the large intestine in adult Xenopus laevis: scanning electron microscopy of vascular corrosion casts and correlative light microscopy.","authors":"A Lametschwandtner,&nbsp;H Bartel,&nbsp;U Lametschwandtner,&nbsp;S Tholo,&nbsp;B Minnich","doi":"10.1679/aohc.73.55","DOIUrl":"https://doi.org/10.1679/aohc.73.55","url":null,"abstract":"<p><p>The microvascular anatomy of the large intestine of the adult South African Clawed Toad, Xenopus laevis (Daudin), was studied by scanning electron microscopy (SEM) of vascular corrosion casts (VCCs) and correlative light microscopy. Observations showed the large intestine to be supplied by the haemorrhoidal artery and the posterior mesenteric artery and drain via the posterior haemorrhoidal vein into either the left or right posterior abdominal vein. Both arteries and veins showed a bipinnate supply/draining pattern with branches running circumferentially. Vessels embraced the gut wall while arteries and veins in most cases alternated along the gut length. Many short terminal arterioles arose from the circumferential arteries at almost acute angles and capillarized after a short distance. Capillary lengths were short and continued into numerous postcapillary venules which merged either in a leaf vein-like formation or in a rosette-like formation with up to four draining sites per supplying arteriole. The microvasculature was found to be well adapted 1) to sustain blood flow under different amounts of feces in the gut and 2) to provide optimal conditions for the resorption of water and salts from the gut lumen into the blood vascular system by the high number of venules and their conspiciouos rosette-like and leaf vein-like patterns.</p>","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":"73 1","pages":"55-64"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.73.55","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29797052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scale and tooth phenotypes in medaka with a mutated ectodysplasin-A receptor: implications for the evolutionary origin of oral and pharyngeal teeth.","authors":"A D S Atukorala,&nbsp;Keiji Inohaya,&nbsp;Otto Baba,&nbsp;Makoto J Tabata,&nbsp;R A R K Ratnayake,&nbsp;Dawud Abduweli,&nbsp;Shohei Kasugai,&nbsp;Hiroshi Mitani,&nbsp;Yoshiro Takano","doi":"10.1679/aohc.73.139","DOIUrl":"https://doi.org/10.1679/aohc.73.139","url":null,"abstract":"<p><p>Ectodermal contribution to the induction of pharyngeal teeth that form in the endodermal territory of the oropharyngeal cavity in some teleost fishes has been a matter of considerable debate. To determine the role of ectodermal cell signaling in scale and tooth formation and thereby to gain insights in evolutionary origin of teeth, we analyzed scales and teeth in rs-3 medaka mutants characterized by reduced scale numbers due to aberrant splicing of the ectodysplasin-A receptor (edar). Current data show that, in addition to a loss of scales (83% reduction), a drastic loss of teeth occurred in both oral (43.5% reduction) and pharyngeal (73.5% reduction) dentitions in rs-3. The remaining scales of rs-3 were irregular in shape and nearly 3 times larger in size relative to those of the wild-type. In contrast, there was no abnormality in size and shape in the remaining teeth of rs-3. In wild-type medaka embryos, there was a direct contact between the surface ectoderm and rostral endoderm in pharyngeal regions before the onset of pharyngeal tooth formation. However, there was no sign of ectodermal cell migration in the pharyngeal endoderm and hence no direct evidence of any ectodermal contribution to pharyngeal odontogenesis. These data suggest differential roles for Eda-Edar signaling in the induction and growth of scales and teeth and support the intrinsic odontogenic competence of the rostral endoderm in medaka.</p>","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":"73 3","pages":"139-48"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.73.139","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30606231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recruited peripheral blood monocytes participate in the liver extramedullary hematopoietic milieu.","authors":"Ayako Nakamura-Ishizu,&nbsp;Shunichi Morikawa,&nbsp;Kazuhiko Shimizu,&nbsp;Taichi Ezaki","doi":"10.1679/aohc.73.127","DOIUrl":"https://doi.org/10.1679/aohc.73.127","url":null,"abstract":"<p><p>The hematopoietic microenvironment has been investigated and well defined in the bone marrow. However, there is a lack of studies on the extramedullary hematopoietic milieu such as in the liver, to which hematopoietic stem cells migrate and there commence hematopoiesis under pathological conditions such as bone marrow failure. We induced extramedullary hematopoiesis by phenylhydrazine in the adult mouse liver and investigated the immunohistochemical, ultrastructural, and molecular changes within this organ. Using an intravital lectin injection technique, we found numerous monocytes attached to the central vein prior to hematopoietic foci formation. These cells were later incorporated into the hematopoietic foci. An increase in the mRNA expressions of the monocyte attracting chemokine CCL-2 (MCP-1) was noted in the central vein region as well as in cells within the hematopoietic foci. Together with local liver components, we regard these monocytes as components of the extramedullary hematopoietic milieu. We conclude that the recruitment of extra-hepatic monocytes is an important event during extramedullary hematopoiesis in the liver and that these monocytes participate in the liver hematopoietic microenvironment.</p>","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":"73 3","pages":"127-37"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.73.127","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30606230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondria of human Leydig cells as seen by high resolution scanning electron microscopy.","authors":"Alessandro Riva,&nbsp;Bernard Tandler,&nbsp;Tatsuo Ushiki,&nbsp;Paolo Usai,&nbsp;Raffaella Isola,&nbsp;Gabriele Conti,&nbsp;Francesco Loy,&nbsp;Charles L Hoppel","doi":"10.1679/aohc.73.37","DOIUrl":"https://doi.org/10.1679/aohc.73.37","url":null,"abstract":"<p><p>The three-dimensional ultrastructure of over 1000 mitochondria in human Leydig cells (from twelve sexually mature patients) was examined by high resolution scanning electron microscopy (HRSEM) of osmium-macerated specimens, as well as by transmission electron microscopy of conventional ultrathin sections. The stereo-pair imaging of the osmium-macerated specimens by HRSEM is also very useful for investigating the three-dimensional structure of cytoplasmic membranous organelles with great clarity. The mitochondria, which mainly are elongated (although some are ovate), possess cristae that are almost exclusively tubular and that occasionally display constrictions and terminal bulbules. Lamelliform cristae are quite rare. Occasionally, the tubular cristae are joined together to form a simple network. Classic crista junctions could not be identified with certainty, although the base of the tubular cristae might correspond functionally to such junctions. As a whole, in line with the identical and common embryological origin of adrenal cortex and gonads, mitochondria of human Leydig cell closely resemble those of steroidogenic cells of human suprarenal cortex treated by the same maceration method.</p>","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":"73 1","pages":"37-44"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.73.37","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29797050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liver reconstruction on the chorioallantoic membrane of the chick embryo.","authors":"Akina Chiba,&nbsp;Chisato Yui,&nbsp;Shigeki Hirano","doi":"10.1679/aohc.73.45","DOIUrl":"https://doi.org/10.1679/aohc.73.45","url":null,"abstract":"<p><p>The liver from a 6-day-old chick embryo was transplanted on the chorioallantoic membrane of a 9-day-old chick embryo to observe the process of liver regeneration histologically. When a piece of the liver was implanted on the chorioallantoic membrane, only cells in the superficial zone of the graft adhering to the chorioallantoic membrane survived. Eventually, these surviving cells in the superficial zone proliferated with hematopoiesis, resulting in the formation of clusters of blood cells surrounded by the hepatocytes (or hepatic parenchymal cells). Semi-thin serial sections showed that these clusters of blood cells were confined to the space formed by hepatocytes. Furthermore, structures similar to the hepatic cord, sinusoid, central vein, and bile duct appeared in the reconstructed liver eleven days after transplantation. Meanwhile, when a pellet of the dissociated liver cells was transplanted onto the chorioallantoic membrane, two types of liver like structures were reconstructed: one was clusters of hepatocytes accompanied by sinusoids after hematopoiesis, and the other was a simple accumulation of hepatocytes without any sinusoids or hematopoiesis. The sinusoids found in the former type became clear following the connection between the space in the transplant and vessels of the chorioallantoic membrane. These findings indicated that the reconstructed liver was primarily produced by the accumulation of hepatocytes accompanied by hematopoietic cells, followed by the formation of sinusoidal spaces. We therefore consider that hematopoiesis is important for liver regeneration with a normal structure. Transplantation of the liver in the chorioallantoic membrane could be also useful for research into liver regeneration.</p>","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":"73 1","pages":"45-53"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.73.45","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29797051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The initial process of enamel prism arrangement and its relation to the Hunter-Schreger bands in dog teeth.","authors":"Yoshinori Hanaizumi,&nbsp;Rumi Yokota,&nbsp;Takanori Domon,&nbsp;Minoru Wakita,&nbsp;Yukisige Kozawa","doi":"10.1679/aohc.73.23","DOIUrl":"https://doi.org/10.1679/aohc.73.23","url":null,"abstract":"<p><p>The three-dimensional architecture of enamel prisms at early stages of enamel formation and its spatial relationship to the Hunter-Schreger bands were examined in canine tooth germs by light and electron microscopy. In serial semithin sections of demineralized tooth germs tangential to the enamel-dentin junction, a straight row of enamel prisms was depicted along the longitudinal tooth axis at the level of the enamel-dentin junction and then their three-dimensional arrangement was reconstructed using computer software. The spatial arrangement of the groups of enamel rods oriented in specific sideward directions was also reconstructed in deep layers of the enamel. Initially, all enamel prisms were parallel to perpendicular toward the enamel-dentin junction, but at 10µm from the enamel-dentin junction, some small specks, or groups of enamel prisms--tilting to the right or the left--emerged as small islands. In each speck of enamel prism, the inclined prisms were uniformly oriented in a sideward direction and gradually expanded their boundary until merging with the neighboring specks inclined in the same direction. Consequently, at 50µm from the enamel-dentin junction, the group of enamel prisms oriented either to the right or the left formed alternately arranged horizontal belt-like zones, corresponding to the parazone or the diazone of the Hunter-Schreger bands. Reversed images of scanning electron-micrographs of the exposed surfaces of the developing enamel revealed round and bulb-like profiles of Tomes' processes at early amelogenesis and its changes into a characteristic structure combined with flat secretory and enclosing nonsecretory faces that dictated the orientation of corresponding enamel prisms. The results suggest that the groups of enamel prisms oriented in sideward directions first appear as small island-like specks near the enamel-dentin junction, which later merge and form alternating horizontal belt-like zones as a consequence of morphological changes of the Tomes' processes. However, the mechanisms whereby the functional grouping of secretory ameloblasts with similarly oriented Tomes' processes is induced are yet to be determined.</p>","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":"73 1","pages":"23-36"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.73.23","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29798174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid three-dimensional analysis of renal biopsy sections by low vacuum scanning electron microscopy.","authors":"Sumire Inaga,&nbsp;Masako Kato,&nbsp;Sayuri Hirashima,&nbsp;Chishio Munemura,&nbsp;Sinichi Okada,&nbsp;Toshio Kameie,&nbsp;Tetsuo Katsumoto,&nbsp;Hironobu Nakane,&nbsp;Keiichi Tanaka,&nbsp;Kazuhiko Hayashi,&nbsp;Tomonori Naguro","doi":"10.1679/aohc.73.113","DOIUrl":"https://doi.org/10.1679/aohc.73.113","url":null,"abstract":"<p><p>Renal biopsy paraffin sections were examined by low vacuum scanning electron microscopy (LVSEM) in the backscattered electron (BSE) mode, a novel method for rapid pathological analysis which allowed detailed and efficient three-dimensional observations of glomeruli. Renal samples that had been already diagnosed by light microscopy (LM) as exhibiting IgA nephropathy, minor glomerular abnormalities, and membranous glomerulonephritis (GN) were rapidly processed in the present study. Unstained paraffin sections of biopsy samples on glass slides were deparaffinized, stained with platinum blue (Pt-blue) or periodic acid silver-methenamine (PAM), and directly observed with a LVSEM. Overviews of whole sections and detailed observations of individual glomeruli were immediately performed at arbitrary magnifications between ×50 to ×18,000. Cut surface views and surface views of glomeruli were demonstrated at the same time. On Pt-blue-stained sections, podocytes, endothelia, mesangium, and glomerular basement membranes (GBMs) could be distinguished due to the different yields of BSE signals, and pathological features were investigated in every sample. The abnormal surface appearances of podocytes with foot processes and the varying thicknesses of GBM were revealed three-dimensionally, features difficult to observe under LM and transmission electron microscopy. PAM-positive GBM alterations in membranous GN were distinctly visualized through overlying cells without cell removal under LVSEM at high magnification. Not only prominent spike formation but also slight protrusions were clearly revealed in the side views of GBM. Crater-like or hole-like structures were shown in the en face views of GBM. Accordingly, LVSEM is expected to provide a novel approach to the pathological diagnosis of human glomerular diseases using conventional renal biopsy sections.</p>","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":"73 3","pages":"113-25"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.73.113","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30606229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fixation conditions affect the immunoreactivity of gustducin in rat vallate taste buds.","authors":"Maiko Haga,&nbsp;Sumio Yoshie","doi":"10.1679/aohc.73.91","DOIUrl":"https://doi.org/10.1679/aohc.73.91","url":null,"abstract":"<p><p>This study aimed to examine gustducin immunoreactivities when adopting various immunostaining conditions in rat vallate taste buds. The occurrence and intensity of the immunoreactivities exhibited specific patterns in accordance with the fixation time. The immunoreactions were localized to only taste hairs, the upper part of the taste bud, after short fixation periods but then to the cell-body cytoplasm excluding the taste hairs after long fixation periods. These immunohistochemical data suggest that the staining protocols, especially the fixation time, cause discrepancies in gustducin immunoreactivities.</p>","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":"73 2","pages":"91-3"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.73.91","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29877862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}